FastQCFastQC Report
Wed 25 May 2016
SRR2049498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6033543
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194630.32257995012217533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193150.32012699669166195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119780.1985234877749276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118150.056.6568681
GTATCAA207750.039.2005161
ATCAACG262200.030.5773723
TCAACGC264250.030.3579644
TATCAAC269950.029.821532
CAACGCA273900.029.3597985
GTGGTAT51200.028.318321
AACGCAG287550.028.0798056
TGGTATC51800.025.88682
ACGCAGA321250.024.9726077
CGCAGAG320550.024.7778978
GTACATG154800.022.9595171
GCAGAGT346050.022.5853569
TACATGG155550.022.0203722
GAGTACT252850.021.57027412-13
ACATGGG155800.020.8978633
AGAGTAC319400.020.835410-11
CAGAGTA336950.020.69167110-11
TATAACG4600.020.4566922
ATAACGC4700.020.0214443
Produced by FastQC (version 0.11.5)