##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049498_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6033543
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669249560332958	31.0	31.0	34.0	30.0	34.0
2	31.94752187893581	33.0	31.0	34.0	30.0	34.0
3	31.926995133705024	33.0	31.0	34.0	30.0	34.0
4	35.5940526155196	37.0	35.0	37.0	33.0	37.0
5	35.72893488950025	37.0	35.0	37.0	33.0	37.0
6	35.81111181274419	37.0	35.0	37.0	35.0	37.0
7	35.79634453587221	37.0	35.0	37.0	35.0	37.0
8	35.784097171429785	37.0	35.0	37.0	33.0	37.0
9	37.51439494174484	39.0	37.0	39.0	35.0	39.0
10-11	37.47374950340124	39.0	37.0	39.0	34.5	39.0
12-13	37.4623431870793	39.0	37.0	39.0	34.5	39.0
14-15	38.72868080993207	40.0	38.0	41.0	34.5	41.0
16-17	38.733028752757704	40.0	38.0	41.0	34.5	41.0
18-19	38.8109178968311	40.0	38.0	41.0	35.0	41.0
20-21	38.84185063403045	40.0	38.0	41.0	35.0	41.0
22-23	38.81288382298759	40.0	38.0	41.0	35.0	41.0
24-25	38.78197677550321	40.0	38.0	41.0	35.0	41.0
26-27	38.690212450628096	40.0	38.0	41.0	34.5	41.0
28-29	38.604186213639316	40.0	38.0	41.0	34.0	41.0
30-31	38.487989478155704	40.0	38.0	41.0	34.0	41.0
32-33	38.340559518677495	40.0	38.0	41.0	34.0	41.0
34-35	38.23788618064046	40.0	38.0	41.0	34.0	41.0
36-37	38.10240798814229	40.0	38.0	41.0	33.5	41.0
38-39	38.01496566776768	40.0	38.0	41.0	33.0	41.0
40-41	37.898313561368504	40.0	37.5	41.0	33.0	41.0
42-43	37.76340385740186	40.0	37.0	41.0	33.0	41.0
44-45	37.581852321264634	40.0	37.0	41.0	33.0	41.0
46-47	37.332306656304596	40.0	37.0	41.0	32.5	41.0
48-49	37.09505219404254	40.0	37.0	41.0	31.5	41.0
50-51	36.891739066084384	40.0	37.0	41.0	31.0	41.0
52-53	36.79904295038587	40.0	36.0	41.0	31.0	41.0
54-55	36.67844755560705	40.0	36.0	41.0	31.0	41.0
56-57	36.51831585521144	39.5	36.0	41.0	31.0	41.0
58-59	36.359758271383825	39.0	35.0	41.0	30.0	41.0
60-61	36.18616027100495	39.0	35.0	41.0	30.0	41.0
62-63	35.99686701495291	39.0	35.0	41.0	30.0	41.0
64-65	35.73415089608211	38.5	35.0	40.5	29.0	41.0
66-67	35.48739049344639	38.0	35.0	40.0	29.0	41.0
68-69	35.18458673452729	37.0	35.0	40.0	29.0	41.0
70-71	34.89855164701735	37.0	34.0	40.0	29.0	41.0
72-73	34.58695181255856	36.5	34.0	39.0	28.5	41.0
74-75	34.24560850896397	36.0	34.0	39.0	28.0	41.0
76-77	33.94668804050953	35.5	34.0	38.5	28.0	40.0
78-79	33.61041091444943	35.0	34.0	37.5	28.0	39.5
80-81	33.292624002182464	35.0	34.0	37.0	27.5	39.0
82-83	32.98850708447756	35.0	34.0	36.5	27.0	39.0
84-85	32.700933017300116	35.0	33.5	36.0	27.0	37.5
86-87	32.422717630420465	35.0	33.0	36.0	26.0	37.0
88-89	32.19376575256031	35.0	33.0	35.5	26.0	37.0
90-91	32.03051730964708	35.0	33.0	35.0	26.0	36.0
92-93	31.86647563463126	35.0	33.0	35.0	26.0	36.0
94-95	31.679614200147412	35.0	33.0	35.0	25.5	36.0
96-97	31.505541022911416	35.0	33.0	35.0	25.0	36.0
98-99	31.323831370721976	35.0	33.0	35.0	25.0	35.5
100	31.213462305646946	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47769925088099185
1101	2	0.49325139357351944
1101	3	0.5706361721598974
1101	4	0.42109419339854526
1101	5	0.3599667010378482
1101	6	0.3390647189766014
1101	7	0.338845480647727
1101	8	0.3399916903028597
1101	9	0.39052713516350224
1101	10-11	0.39658551933445807
1101	12-13	0.3729840116766212
1101	14-15	0.5107345341960254
1101	16-17	0.5425954421524821
1101	18-19	0.5266337125173592
1101	20-21	0.47993596286625007
1101	22-23	0.4619484174761439
1101	24-25	0.522666325128263
1101	26-27	0.5560404217447541
1101	28-29	0.5510242492205606
1101	30-31	0.5651463100154217
1101	32-33	0.5548878416025076
1101	34-35	0.5640090692038129
1101	36-37	0.5515802013460842
1101	38-39	0.6037557967963139
1101	40-41	0.6002714943554182
1101	42-43	0.5683100779378094
1101	44-45	0.5925761689461808
1101	46-47	0.6301939248664752
1101	48-49	0.5901272286774741
1101	50-51	0.6468513453759641
1101	52-53	0.6320323247666693
1101	54-55	0.591482780408576
1101	56-57	0.5393071678099091
1101	58-59	0.5375390951276131
1101	60-61	0.5663843947288782
1101	62-63	0.5857449278477347
1101	64-65	0.5827452642595219
1101	66-67	0.5816449230667686
1101	68-69	0.5698574842288124
1101	70-71	0.5607909415159114
1101	72-73	0.5944666716215323
1101	74-75	0.5418432819205208
1101	76-77	0.4778025081913171
1101	78-79	0.43687489739111385
1101	80-81	0.48795755439738997
1101	82-83	0.4630826353044526
1101	84-85	0.4903570297949713
1101	86-87	0.5435155824748605
1101	88-89	0.5092439771580999
1101	90-91	0.5553774313401192
1101	92-93	0.5226199770519067
1101	94-95	0.5207763767314262
1101	96-97	0.5488722910002579
1101	98-99	0.6294724943280485
1101	100	0.676526683380569
1102	1	0.393112864158514
1102	2	0.4208243039145465
1102	3	0.49460388273848466
1102	4	0.4149422120758999
1102	5	0.36754476245867096
1102	6	0.302930418473224
1102	7	0.34778503506184677
1102	8	0.3562029741864663
1102	9	0.43370079455606003
1102	10-11	0.38155257276110177
1102	12-13	0.346388781684567
1102	14-15	0.4670813175357651
1102	16-17	0.5074626787109722
1102	18-19	0.5290187482650879
1102	20-21	0.49010410224601486
1102	22-23	0.49867329674672334
1102	24-25	0.46425555082340964
1102	26-27	0.46060530803173094
1102	28-29	0.48378716640321073
1102	30-31	0.5032966497292932
1102	32-33	0.5270646621882733
1102	34-35	0.578923208870215
1102	36-37	0.5643156582843574
1102	38-39	0.5753531209040901
1102	40-41	0.5920648010022731
1102	42-43	0.5810412052362111
1102	44-45	0.6579121426448538
1102	46-47	0.6899406512615229
1102	48-49	0.7347090776195913
1102	50-51	0.7523503296186149
1102	52-53	0.7151222052304718
1102	54-55	0.6421609370655901
1102	56-57	0.6704834983916541
1102	58-59	0.6655882340774326
1102	60-61	0.6667531529373107
1102	62-63	0.6550489171858516
1102	64-65	0.6458652170513588
1102	66-67	0.6638614079203364
1102	68-69	0.6362604559665144
1102	70-71	0.6297632168854577
1102	72-73	0.6769969163407197
1102	74-75	0.607682844374267
1102	76-77	0.5943410413506314
1102	78-79	0.5773119701668818
1102	80-81	0.5598944414355742
1102	82-83	0.5806311264765966
1102	84-85	0.587085077249732
1102	86-87	0.5578891527006604
1102	88-89	0.5503143848878338
1102	90-91	0.5769645788081306
1102	92-93	0.6044899318298071
1102	94-95	0.5664042666667441
1102	96-97	0.5858474552326314
1102	98-99	0.659900488365377
1102	100	0.6956196802317685
1103	1	0.34542109821046196
1103	2	0.3427637955873699
1103	3	0.44785755624083023
1103	4	0.34786500428166534
1103	5	0.27086593374632884
1103	6	0.30751964060007
1103	7	0.3039534109507045
1103	8	0.3102698788937346
1103	9	0.40348704971437854
1103	10-11	0.3405775016666013
1103	12-13	0.3214659592279716
1103	14-15	0.4164129311248743
1103	16-17	0.4261167689260148
1103	18-19	0.4133085717054925
1103	20-21	0.4072664549831728
1103	22-23	0.38911270060110326
1103	24-25	0.41839385860642864
1103	26-27	0.45367950055728556
1103	28-29	0.4386014005393335
1103	30-31	0.4500199137669938
1103	32-33	0.43731439792354365
1103	34-35	0.4932821498809048
1103	36-37	0.5443056355486817
1103	38-39	0.5132234026353828
1103	40-41	0.5368894590263125
1103	42-43	0.48996244665721633
1103	44-45	0.5087494986296832
1103	46-47	0.45542177646674986
1103	48-49	0.4744352676910921
1103	50-51	0.4668327627926203
1103	52-53	0.47530359332448313
1103	54-55	0.44011704362457493
1103	56-57	0.4998627883936706
1103	58-59	0.468024384096438
1103	60-61	0.398596714084654
1103	62-63	0.44099911380609313
1103	64-65	0.4606226848569932
1103	66-67	0.43872490486712934
1103	68-69	0.4179609934396993
1103	70-71	0.43332136645766894
1103	72-73	0.4846058309451706
1103	74-75	0.4420888046770912
1103	76-77	0.3666535413759249
1103	78-79	0.35607614151484057
1103	80-81	0.3636355250302472
1103	82-83	0.37549294799308797
1103	84-85	0.38741586091403946
1103	86-87	0.37170097870924934
1103	88-89	0.3975006990099672
1103	90-91	0.3643204144047161
1103	92-93	0.3112455430037109
1103	94-95	0.3393365924351386
1103	96-97	0.38765382658245073
1103	98-99	0.41844944482321367
1103	100	0.4332038412640955
1104	1	0.3655590443314587
1104	2	0.34376392495046915
1104	3	0.4318304859225357
1104	4	0.3289550749129475
1104	5	0.28995199315257736
1104	6	0.30906124173730376
1104	7	0.20413350182464995
1104	8	0.2559210985406466
1104	9	0.3203650821162043
1104	10-11	0.27701355075287637
1104	12-13	0.29387740728069645
1104	14-15	0.3823701720183479
1104	16-17	0.36750008438107784
1104	18-19	0.38567674126127116
1104	20-21	0.3728980533490329
1104	22-23	0.41216922597613603
1104	24-25	0.3577934612146194
1104	26-27	0.41410727452362295
1104	28-29	0.42123187921186656
1104	30-31	0.4046117586591862
1104	32-33	0.38404607196923735
1104	34-35	0.36316882514270077
1104	36-37	0.3475676992631591
1104	38-39	0.38658196298400327
1104	40-41	0.38955950487996205
1104	42-43	0.42887118414121517
1104	44-45	0.3956135603014772
1104	46-47	0.4302953506867624
1104	48-49	0.34850163071785545
1104	50-51	0.39376822489988683
1104	52-53	0.36298662151594385
1104	54-55	0.3395639073689054
1104	56-57	0.38987832528928124
1104	58-59	0.396504741981623
1104	60-61	0.3206348442210256
1104	62-63	0.34954372808604006
1104	64-65	0.35842018727709757
1104	66-67	0.39171360424958124
1104	68-69	0.30643319103286615
1104	70-71	0.35640680472724995
1104	72-73	0.3482653082698022
1104	74-75	0.3908391244510412
1104	76-77	0.3388262127675006
1104	78-79	0.360768248863927
1104	80-81	0.37689013704269314
1104	82-83	0.38252354163888214
1104	84-85	0.4437455500356222
1104	86-87	0.4349579405757211
1104	88-89	0.38983919378554077
1104	90-91	0.4257181208987433
1104	92-93	0.4291043245293089
1104	94-95	0.4217894936085216
1104	96-97	0.5033227197963619
1104	98-99	0.5367464680669016
1104	100	0.5774144065953664
1105	1	0.31103751565165894
1105	2	0.2802181202692289
1105	3	0.37545190003780604
1105	4	0.3081200076797188
1105	5	0.21460451610625597
1105	6	0.24334686533538985
1105	7	0.22908580752132934
1105	8	0.2311815430187636
1105	9	0.24709235768116145
1105	10-11	0.2493999914890992
1105	12-13	0.2565671783497976
1105	14-15	0.34998463966153537
1105	16-17	0.3071838666344675
1105	18-19	0.29209130282878704
1105	20-21	0.3181060275173877
1105	22-23	0.36505945954713326
1105	24-25	0.333173099802373
1105	26-27	0.34532122540765187
1105	28-29	0.369204956810087
1105	30-31	0.3550877021243153
1105	32-33	0.38541625183712824
1105	34-35	0.3771963602621824
1105	36-37	0.36727574471650115
1105	38-39	0.35822558434550444
1105	40-41	0.3687509349690288
1105	42-43	0.35468671332571944
1105	44-45	0.36782428749076246
1105	46-47	0.4297381703946712
1105	48-49	0.39015991065759437
1105	50-51	0.44013866167431104
1105	52-53	0.45064167513049824
1105	54-55	0.4163020992694868
1105	56-57	0.4221149220019669
1105	58-59	0.4249024887096624
1105	60-61	0.38902334582112275
1105	62-63	0.3861309361047418
1105	64-65	0.36897888796485745
1105	66-67	0.40542410092444214
1105	68-69	0.41760781855784757
1105	70-71	0.4180126055890909
1105	72-73	0.3952255077485205
1105	74-75	0.4085682180023724
1105	76-77	0.3833785404195993
1105	78-79	0.34793642514143386
1105	80-81	0.372251632592949
1105	82-83	0.38310923592572266
1105	84-85	0.35684699409585363
1105	86-87	0.4237174643113448
1105	88-89	0.372825738371624
1105	90-91	0.3658452028280763
1105	92-93	0.37543467336278624
1105	94-95	0.38908525686029094
1105	96-97	0.3487611207118988
1105	98-99	0.4142038228976155
1105	100	0.47032176960675187
1106	1	0.26108663050667147
1106	2	0.3001950015081114
1106	3	0.4112199962087466
1106	4	0.31033995622684074
1106	5	0.25090487287713614
1106	6	0.2569391729184787
1106	7	0.24506618895330945
1106	8	0.23928720497026035
1106	9	0.24207699403154948
1106	10-11	0.25259120582873607
1106	12-13	0.2881351551578959
1106	14-15	0.318550162105808
1106	16-17	0.35861468343028235
1106	18-19	0.3591627776339408
1106	20-21	0.3274269208041005
1106	22-23	0.29455127273357107
1106	24-25	0.32649772861673654
1106	26-27	0.320586275558739
1106	28-29	0.36884603074058475
1106	30-31	0.3590484523227033
1106	32-33	0.4001999406594621
1106	34-35	0.3475351654305925
1106	36-37	0.3775774540482786
1106	38-39	0.4307513769219824
1106	40-41	0.41278459598327544
1106	42-43	0.4639812336154989
1106	44-45	0.3902439905504309
1106	46-47	0.37153111744633804
1106	48-49	0.4478277062968985
1106	50-51	0.5098298677915452
1106	52-53	0.4975853032070958
1106	54-55	0.46693421874018526
1106	56-57	0.4849641637578799
1106	58-59	0.44706939096624865
1106	60-61	0.4879121254183545
1106	62-63	0.47284917004957805
1106	64-65	0.4822457515403684
1106	66-67	0.46448950197324734
1106	68-69	0.468481962878208
1106	70-71	0.49766181408764965
1106	72-73	0.48436945477291715
1106	74-75	0.4474479090276944
1106	76-77	0.47515830607989784
1106	78-79	0.43018835835258784
1106	80-81	0.381352461503802
1106	82-83	0.37940057153824824
1106	84-85	0.4139624072127077
1106	86-87	0.502165792388972
1106	88-89	0.5438627436085355
1106	90-91	0.5131501368766891
1106	92-93	0.5196087077684162
1106	94-95	0.5137609951433362
1106	96-97	0.5561013426883648
1106	98-99	0.6116751100888251
1106	100	0.7018307187041088
1107	1	0.24230192992019184
1107	2	0.2347668587300653
1107	3	0.3041229559337104
1107	4	0.22828125891719253
1107	5	0.17421755145323914
1107	6	0.1601513003975441
1107	7	0.15862579321344583
1107	8	0.1837542474362266
1107	9	0.19021461740640433
1107	10-11	0.20017536806246028
1107	12-13	0.1699339934308597
1107	14-15	0.21757053042276908
1107	16-17	0.17839690207523518
1107	18-19	0.21035718985343976
1107	20-21	0.20918273178185842
1107	22-23	0.24169693401773884
1107	24-25	0.2809231148997
1107	26-27	0.29867472067214607
1107	28-29	0.302650018779822
1107	30-31	0.3002228223320742
1107	32-33	0.31140703293510796
1107	34-35	0.2663609199172683
1107	36-37	0.28911689451597056
1107	38-39	0.2636908590230149
1107	40-41	0.2899344004036024
1107	42-43	0.28542537532923973
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2216	6	-1.0800007040079649
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2216	54-55	-1.8443572894192997
2216	56-57	-1.8105941657855738
2216	58-59	-1.7781841556399627
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2216	64-65	-1.8922502803753432
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2216	74-75	-1.771995813772655
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2216	80-81	-1.7158170248049807
2216	82-83	-1.8680383704848111
2216	84-85	-1.9380106435826931
2216	86-87	-2.0777932110403015
2216	88-89	-1.867025889520903
2216	90-91	-1.7331806872113162
2216	92-93	-1.8224138591900783
2216	94-95	-2.001727203769967
2216	96-97	-2.0838014511542298
2216	98-99	-2.118646833405105
2216	100	-2.155495274341039
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	374.0
3	52.0
4	63.0
5	135.0
6	363.0
7	1006.0
8	2120.0
9	3351.0
10	4971.0
11	7073.0
12	10533.0
13	15178.0
14	17283.0
15	22129.0
16	29013.0
17	41399.0
18	42011.0
19	22181.0
20	16180.0
21	15539.0
22	15804.0
23	17763.0
24	20402.0
25	25077.0
26	32562.0
27	41722.0
28	55023.0
29	72452.0
30	93878.0
31	121537.0
32	155921.0
33	202622.0
34	275409.0
35	395768.0
36	633694.0
37	1179516.0
38	1721425.0
39	719449.0
40	2565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24957754065145	21.015839364952807	15.081210036340625	22.653373058055116
2	15.421965670477153	21.145768211081947	40.09471081908351	23.33755529935739
3	17.89747748545092	25.7172444117826	31.305851304946366	25.07942679782012
4	12.503250311756132	17.91055772611757	38.57467503105116	31.01151693107514
5	12.186478245469868	37.81742500496815	35.8602639658052	14.135832783756777
6	29.57431567209276	38.9296616622231	17.566302993417686	13.929719672266458
7	25.479457095109787	33.431716654708524	22.95483764680222	18.133988603379475
8	24.230704910862492	36.53571375889755	20.78208442369599	18.451496906543966
9	24.16380308929903	16.903517787327733	22.4068071514271	36.52587197194614
10-11	23.127828872687243	27.465727848463168	29.15541498585491	20.25102829299468
12-13	24.110800237936484	25.020978552734274	29.5188581568077	21.349363052521543
14-15	21.28300777271589	27.08657320800505	27.5035339943824	24.12688502489666
16-17	19.975543391337393	30.32153744491421	28.23789438477525	21.465024778973152
18-19	19.778751887572525	28.48108482859905	31.466304292519336	20.273858991309087
20-21	20.946970958854525	27.74274584601452	31.262195363487095	20.048087831643862
22-23	20.558202701132654	27.65102527652492	31.384055769553644	20.406716252788783
24-25	20.450695387436536	27.729262889151528	31.37124406008211	20.44879766332982
26-27	20.270179561163314	27.598593396947695	31.46021334396722	20.67101369792177
28-29	20.29388748732397	27.654806259052013	31.557983393976347	20.493322859647666
30-31	20.672942816716386	28.06592957454916	31.07861087598982	20.182516732744634
32-33	20.2362857113971	27.810309796416465	31.509620466780465	20.443784025405968
34-35	20.401835206942255	27.53570331727146	31.45008662406152	20.612374851724766
36-37	20.787711299977477	27.886641397931534	30.901039405868158	20.42460789622283
38-39	20.65332922960854	27.971168847226245	30.999745920431828	20.375756002733386
40-41	20.512184963296026	27.785796836120998	31.055790934116157	20.64622726646682
42-43	20.48014740261236	27.838510473862538	30.883338363545263	20.79800375997983
44-45	20.78445450707818	27.99204381240011	30.579992551640057	20.643509128881654
46-47	20.821165938487553	28.284981146235307	30.116417501292357	20.777435413984787
48-49	20.847941251102377	28.16738854765765	29.98709050387144	20.99757969736853
50-51	20.953948616923753	28.16949344688519	29.706940018493277	21.169617917697778
52-53	20.935501744165908	28.311764745854965	29.61855082494647	21.134182685032656
54-55	21.059384179411666	28.641512955157527	29.445493303022786	20.853609562408025
56-57	20.878777196085284	28.34572489165984	29.470047698342416	21.305450213912454
58-59	20.67746553759887	28.008494858401345	29.87749961382487	21.43653999017491
60-61	20.961481504316783	28.280307275509596	29.546478743915472	21.211732476258145
62-63	20.7990562095936	28.275583682754892	29.682932565492614	21.242427542158897
64-65	20.721282669237628	28.048552898355076	29.861807564808935	21.368356867598358
66-67	20.66188970560084	28.09598771401812	29.63630158929836	21.605820991082687
68-69	21.115810395318306	28.245675882313257	29.41066302171046	21.22785070065797
70-71	21.031216649984927	28.005551630277598	29.47592318476888	21.48730853496859
72-73	20.82608013235341	28.1624494927773	29.634718771375297	21.376751603494
74-75	20.915712376625144	28.17709263031688	29.410290106492987	21.49690488656499
76-77	20.9623184918049	28.21820446129248	29.21605100021662	21.603426046686003
78-79	21.04947684522493	28.250830213343264	29.34988657984256	21.349806361589245
80-81	20.916166758058875	28.09035422857256	29.37976748065536	21.613711532713204
82-83	20.767338586808933	28.105017137978688	29.550108274103902	21.57753600110847
84-85	20.818671550032875	28.05417148763173	29.35001872034392	21.77713824199148
86-87	20.925501063429984	28.176274551807666	29.208512246329583	21.68971213843277
88-89	20.736087001667926	27.966386894458882	29.328290632720645	21.969235471152544
90-91	20.573035209769525	27.909782957380646	29.33386436499908	22.183317467850745
92-93	20.686100026717458	28.08205442008198	29.18402528015211	22.047820273048448
94-95	20.532084108380854	27.852054719142334	29.34502704151371	22.270834130963102
96-97	20.491740922373474	27.88514145005679	29.32485108666666	22.298266540903082
98-99	20.464037657491485	27.844339488563968	29.26855301625969	22.423069837684857
100	20.54885111004005	27.81855766062548	29.156791209578024	22.475800019756438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	985.0
1	801.0
2	1400.5
3	2263.5
4	1502.0
5	784.0
6	1725.0
7	4531.5
8	7377.5
9	8295.5
10	6600.0
11	4314.5
12	3984.5
13	4463.0
14	4908.5
15	5483.5
16	6347.5
17	7227.0
18	8145.5
19	9257.5
20	11035.5
21	13422.0
22	17166.5
23	23123.0
24	30246.0
25	39294.5
26	52224.0
27	68350.5
28	87418.0
29	107269.0
30	124792.5
31	141092.5
32	156442.0
33	169724.0
34	182654.0
35	194820.0
36	203638.0
37	211652.5
38	218042.5
39	220070.5
40	220413.0
41	218287.5
42	213820.5
43	209922.0
44	205111.5
45	199830.0
46	194847.0
47	189092.5
48	182880.5
49	177899.0
50	173051.5
51	164805.0
52	153904.5
53	144957.0
54	137899.5
55	129972.5
56	119666.0
57	108054.5
58	97210.0
59	86827.0
60	74773.5
61	61020.0
62	49392.0
63	39365.0
64	30666.0
65	23738.0
66	18095.0
67	13506.5
68	9691.0
69	7014.5
70	4974.5
71	3530.5
72	2538.5
73	1671.5
74	1091.5
75	709.5
76	410.5
77	225.5
78	133.0
79	67.5
80	36.0
81	23.0
82	16.0
83	6.5
84	2.0
85	1.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.5
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05597043064083574
2	3.149061836469882E-4
3	0.0
4	0.11929972157321163
5	0.001823141063219405
6	0.0017402710148912504
7	0.0
8	0.0
9	9.944405799378574E-5
10-11	0.0
12-13	0.0
14-15	3.3148019331261914E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.574530918234941E-4
30-31	2.3203613531883342E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8231410632194053E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	5.8009033829708355E-5
80-81	3.8948922714232754E-4
82-83	2.2374913048601793E-4
84-85	0.0
86-87	8.287004832815479E-6
88-89	5.8009033829708355E-5
90-91	7.45830434953393E-5
92-93	5.800903382970835E-4
94-95	6.961084059565002E-4
96-97	0.0
98-99	4.1435024164077395E-5
100	0.0011104586475972741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6033543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.17329649888894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29604608577803	51.028114262390844
2	13.821000421371338	18.015203167466062
3	3.818664885575403	7.466249364525046
4	1.5191041591803174	3.960201031158169
5	0.7560489902850357	2.4637102505766117
6	0.4322212404009979	1.6901569832263077
7	0.2809823718702752	1.281878320300376
8	0.19595367761566512	1.0216757705034751
9	0.13978485524678688	0.8199215835347801
>10	0.6951430890421207	7.812703191615557
>50	0.03162685952304364	1.3950809404247588
>100	0.012277996337263316	1.4177003695559773
>500	6.872298858212878E-4	0.2939969209389527
>1k	3.5632946848141925E-4	0.4002346795081664
>5k	2.5452104849211652E-5	0.09123386667221639
>10k+	7.635631454763495E-5	0.84193929760273
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19463	0.32257995012217533	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19315	0.32012699669166195	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11978	0.1985234877749276	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6574009665630957E-5	0.0	0.0	0.0
3	0.0	1.6574009665630957E-5	0.0	0.0	0.0
4	0.0	1.6574009665630957E-5	0.0	0.0	0.0
5	0.0	3.3148019331261914E-5	0.0	0.0	0.0
6	0.0	3.3148019331261914E-5	0.0	0.0	0.0
7	0.0	3.3148019331261914E-5	0.0	0.0	0.0
8	0.0	3.3148019331261914E-5	0.0	0.0	0.0
9	0.0	3.3148019331261914E-5	0.0	0.0	0.0
10-11	0.0	3.3148019331261914E-5	0.0	0.0	0.0
12-13	0.0	4.1435024164077395E-5	0.0	8.287004832815479E-6	0.0
14-15	0.0	9.944405799378574E-5	0.0	1.6574009665630957E-5	0.0
16-17	0.0	1.6574009665630958E-4	0.0	1.6574009665630957E-5	0.0
18-19	0.0	1.90601111154756E-4	0.0	3.3148019331261914E-5	0.0
20-21	0.0	1.9888811598757147E-4	0.0	5.8009033829708355E-5	0.0
22-23	0.0	1.9888811598757147E-4	0.0	2.1546212565320245E-4	0.0
24-25	0.0	1.9888811598757147E-4	0.0	5.386553141330061E-4	0.0
26-27	0.0	2.0717512082038696E-4	0.0	9.944405799378574E-4	0.0
28-29	0.0	2.1546212565320245E-4	0.0	0.0027098505803306618	0.0
30-31	0.0	2.1546212565320245E-4	0.0	0.007938950629837228	0.0
32-33	0.0	2.320361353188334E-4	0.0	0.019805941550428995	0.0
34-35	0.0	2.4861014498446434E-4	0.0	0.03921410686888284	0.0
36-37	0.0	2.651841546500953E-4	0.0	0.07010806088561894	0.0
38-39	0.0	2.817581643157263E-4	0.0	0.11206516635416372	0.0
40-41	8.287004832815479E-6	2.817581643157263E-4	0.0	0.16081761578561715	0.0
42-43	2.4861014498446434E-5	2.817581643157263E-4	0.0	0.2122219067635716	0.0
44-45	3.3148019331261914E-5	2.817581643157263E-4	0.0	0.2743330079855236	0.0
46-47	3.3148019331261914E-5	2.983321739813572E-4	0.0	0.3533164510470879	0.0
48-49	3.3148019331261914E-5	3.3148019331261916E-4	0.0	0.43680802473770386	0.0
50-51	3.3148019331261914E-5	4.0606323680795846E-4	0.0	0.5221641745157033	0.0
52-53	3.3148019331261914E-5	4.143502416407739E-4	0.0	0.6095755014922409	0.0
54-55	3.3148019331261914E-5	4.143502416407739E-4	0.0	0.7132210709362641	0.0
56-57	3.3148019331261914E-5	4.143502416407739E-4	0.0	0.8398879398058487	0.0
58-59	3.3148019331261914E-5	4.143502416407739E-4	0.0	0.9801703576157492	0.0
60-61	4.972202899689287E-5	4.143502416407739E-4	0.0	1.125955346634639	0.0
62-63	4.972202899689287E-5	4.143502416407739E-4	0.0	1.2771931848335216	0.0
64-65	4.972202899689287E-5	4.143502416407739E-4	0.0	1.4478971973846875	0.0
66-67	4.972202899689287E-5	4.226372464735894E-4	0.0	1.6457411507633242	0.0
68-69	4.972202899689287E-5	4.309242513064049E-4	0.0	1.8682389435195872	0.0
70-71	4.972202899689287E-5	4.3921125613922035E-4	0.0	2.112730447102142	0.0
72-73	4.972202899689287E-5	4.8064628030329776E-4	0.0	2.3736882292875014	0.0
74-75	4.972202899689287E-5	4.889332851361133E-4	0.0	2.6405049238896616	0.0
76-77	4.972202899689287E-5	4.972202899689287E-4	0.0	2.9364255794646694	0.0
78-79	4.972202899689287E-5	4.972202899689287E-4	0.0	3.2712122877055823	0.0
80-81	4.972202899689287E-5	5.137942996345597E-4	0.0	3.639022710205264	0.0
82-83	6.629603866252383E-5	5.137942996345597E-4	0.0	4.02106192000289	0.0
84-85	6.629603866252383E-5	5.303683093001906E-4	0.0	4.406217043617655	0.0
86-87	6.629603866252383E-5	5.469423189658216E-4	0.0	4.823766069123896	0.0
88	6.629603866252383E-5	5.635163286314526E-4	0.0	5.160599667558514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11815	0.0	56.656868	1
GTATCAA	20775	0.0	39.200516	1
ATCAACG	26220	0.0	30.577372	3
TCAACGC	26425	0.0	30.357964	4
TATCAAC	26995	0.0	29.82153	2
CAACGCA	27390	0.0	29.359798	5
GTGGTAT	5120	0.0	28.31832	1
AACGCAG	28755	0.0	28.079805	6
TGGTATC	5180	0.0	25.8868	2
ACGCAGA	32125	0.0	24.972607	7
CGCAGAG	32055	0.0	24.777897	8
GTACATG	15480	0.0	22.959517	1
GCAGAGT	34605	0.0	22.585356	9
TACATGG	15555	0.0	22.020372	2
GAGTACT	25285	0.0	21.570274	12-13
ACATGGG	15580	0.0	20.897863	3
AGAGTAC	31940	0.0	20.8354	10-11
CAGAGTA	33695	0.0	20.691671	10-11
TATAACG	460	0.0	20.456692	2
ATAACGC	470	0.0	20.021444	3
>>END_MODULE