FastQCFastQC Report
Wed 25 May 2016
SRR2049497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6981462
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69050.044.228371
GTATCAA108000.036.248331
TCAACGC114900.033.0839734
ATCAACG116000.032.726083
CAACGCA123500.030.8631155
TATCAAC132800.028.7017672
AACGCAG135300.028.2469316
GTACATG198700.024.060361
ACGCAGA160950.023.0765157
TACATGG205350.022.503272
CGCAGAG163000.022.1367878
ACATGGG208600.021.4653
CATGGGG144800.018.221014
GCAGAGT187500.018.1349289
GAGTACA153050.017.8783021
GTGGTAT42500.017.7233871
CAGAGTA173100.016.23466510-11
AGAGTAC156600.015.64972810-11
GTATAGA28500.015.6420171
AGTACAT163900.015.5975132
Produced by FastQC (version 0.11.5)