##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049497_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6981462 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.19743930426034 31.0 30.0 34.0 27.0 34.0 2 30.364622624888597 31.0 31.0 34.0 27.0 34.0 3 30.450611920540425 31.0 31.0 34.0 27.0 34.0 4 34.11172000936194 37.0 35.0 37.0 30.0 37.0 5 34.13376395946866 37.0 35.0 37.0 30.0 37.0 6 34.236063305937925 37.0 35.0 37.0 30.0 37.0 7 30.383749707439502 36.0 32.0 37.0 2.0 37.0 8 32.09502436595658 36.0 32.0 37.0 17.0 37.0 9 34.83367953589091 38.0 33.0 39.0 28.0 39.0 10-11 35.359409819891596 38.5 35.0 39.0 29.5 39.0 12-13 35.51580406797315 39.0 35.0 39.0 30.0 39.0 14-15 36.64567120754936 40.0 37.0 41.0 30.0 41.0 16-17 36.648995654491856 40.0 37.0 41.0 30.0 41.0 18-19 36.63483529667568 40.0 36.5 41.0 30.0 41.0 20-21 36.69008819356175 40.0 37.0 41.0 30.0 41.0 22-23 36.6463281616372 40.0 37.0 41.0 30.0 41.0 24-25 36.61862357769762 40.0 37.0 41.0 30.0 41.0 26-27 36.62801258532955 40.0 37.0 41.0 30.0 41.0 28-29 36.60690941238383 40.0 36.0 41.0 30.0 41.0 30-31 36.5261059932719 40.0 36.0 41.0 30.0 41.0 32-33 36.48469983794226 40.0 36.0 41.0 30.0 41.0 34-35 36.42842767030745 40.0 36.0 41.0 30.0 41.0 36-37 36.2800360440263 40.0 36.0 41.0 29.5 41.0 38-39 36.16084782814832 40.0 36.0 41.0 29.0 41.0 40-41 36.06995461695559 40.0 35.5 41.0 28.5 41.0 42-43 35.94332576758278 39.5 35.0 41.0 28.0 41.0 44-45 35.815595644579886 39.0 35.0 41.0 28.0 41.0 46-47 35.657037021758484 39.0 35.0 41.0 27.0 41.0 48-49 35.572250482778536 39.0 35.0 41.0 27.0 41.0 50-51 35.437548396023644 39.0 35.0 41.0 27.0 41.0 52-53 35.30690520123149 39.0 35.0 41.0 27.0 41.0 54-55 35.173948737384805 39.0 34.5 40.0 27.0 41.0 56-57 34.9634889511681 39.0 34.0 40.0 26.0 41.0 58-59 34.770409192229366 38.0 34.0 40.0 26.0 41.0 60-61 34.541489232484544 38.0 34.0 40.0 26.0 41.0 62-63 34.29001898169753 38.0 34.0 40.0 25.0 41.0 64-65 33.93258911958556 37.0 33.5 40.0 24.0 41.0 66-67 30.540256467771364 36.0 29.0 39.5 6.5 41.0 68-69 30.86997150453587 35.5 29.0 39.0 10.0 41.0 70-71 31.01805710609039 35.0 29.0 39.0 12.0 41.0 72-73 32.18821150927986 35.0 31.5 39.0 20.0 40.0 74-75 32.252179629424326 35.0 32.0 38.0 20.0 40.0 76-77 31.950285627852736 35.0 32.0 37.0 19.5 39.5 78-79 28.644166150299178 35.0 27.0 36.5 6.0 39.0 80-81 30.379487491301965 35.0 29.0 36.0 12.0 38.5 82-83 30.76719775886483 35.0 31.0 36.0 12.0 37.0 84-85 29.0048901648394 34.5 28.5 35.5 6.0 37.0 86-87 28.64515176047653 34.0 26.0 35.0 7.0 37.0 88-89 29.821065917138846 34.0 30.0 35.0 2.0 36.0 90-91 29.959062371176696 34.0 31.0 35.0 2.0 36.0 92-93 29.808105093173893 34.0 31.0 35.0 2.0 36.0 94-95 29.592758293320223 34.0 31.0 35.0 2.0 35.5 96-97 29.390352264325152 34.0 31.0 35.0 2.0 35.0 98-99 29.182481047665945 34.0 31.0 35.0 2.0 35.0 100 28.958709794596032 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36793214393776097 1101 2 0.35853858371275393 1101 3 0.3789761752482974 1101 4 0.43441811912203576 1101 5 0.4371754623665325 1101 6 0.4681501221433706 1101 7 -11.907156968283164 1101 8 -6.3253498440600495 1101 9 -2.0634302893307392 1101 10-11 -0.042539929585629466 1101 12-13 0.4506518715369623 1101 14-15 0.669283409765626 1101 16-17 0.7373222339397643 1101 18-19 0.7008506196763946 1101 20-21 0.6816965306189573 1101 22-23 0.6539518138667262 1101 24-25 0.7034271736913453 1101 26-27 0.6706296142271526 1101 28-29 0.6568692739548254 1101 30-31 0.7028180705350451 1101 32-33 0.702889279863065 1101 34-35 0.7729610299154714 1101 36-37 0.8691037161258635 1101 38-39 0.8937162166008434 1101 40-41 0.8575316015813286 1101 42-43 0.8812276964469419 1101 44-45 0.7826861453137397 1101 46-47 0.7645908590712267 1101 48-49 0.7111452340517985 1101 50-51 0.8323002069567451 1101 52-53 0.804684371973309 1101 54-55 0.7521367124595031 1101 56-57 0.7551297700914148 1101 58-59 0.7312689698328967 1101 60-61 0.723296960439832 1101 62-63 0.7360470740675211 1101 64-65 0.5968185171617506 1101 66-67 -9.864087814933423 1101 68-69 -7.737378621585055 1101 70-71 -6.300327356571845 1101 72-73 -1.2164637118492578 1101 74-75 0.06234590138369356 1101 76-77 0.3986549644520814 1101 78-79 3.4209430209497675 1101 80-81 1.3668142514797132 1101 82-83 0.6871751404233528 1101 84-85 -4.975925811130583 1101 86-87 -5.480748159000221 1101 88-89 -0.9850322785662406 1101 90-91 0.32585566435155044 1101 92-93 0.6464041261801441 1101 94-95 0.6714806443772439 1101 96-97 0.8513407205912458 1101 98-99 0.8263984759943064 1101 100 0.8501676877570361 1102 1 0.2052714185894331 1102 2 0.2666467922254512 1102 3 0.20786238873190754 1102 4 0.26623408948579197 1102 5 0.2563789596673445 1102 6 0.3240101860953999 1102 7 -12.018883914802743 1102 8 -6.460758829791462 1102 9 -2.229824192566781 1102 10-11 -0.17431749938779717 1102 12-13 0.40645047860196826 1102 14-15 0.5837315669853993 1102 16-17 0.6202699439994461 1102 18-19 0.5470216331869437 1102 20-21 0.5577519606022534 1102 22-23 0.5551059703489685 1102 24-25 0.6117937277897241 1102 26-27 0.5658074376065798 1102 28-29 0.5591584289609557 1102 30-31 0.5218192430547646 1102 32-33 0.5515915421480244 1102 34-35 0.5891905198726803 1102 36-37 0.689136272428577 1102 38-39 0.6904942279671431 1102 40-41 0.5862340463230353 1102 42-43 0.5823543856648925 1102 44-45 0.5955205995804391 1102 46-47 0.6144667931862884 1102 48-49 0.5153215231957091 1102 50-51 0.540685839071088 1102 52-53 0.5547048889522728 1102 54-55 0.48172491416676166 1102 56-57 0.5322633078327357 1102 58-59 0.49898280655310145 1102 60-61 0.44334607892451317 1102 62-63 0.3899079810825796 1102 64-65 0.2800496606097127 1102 66-67 -10.007845254865888 1102 68-69 -6.496938795490788 1102 70-71 -6.035362950215092 1102 72-73 -1.0672130353720632 1102 74-75 -0.15737157374975652 1102 76-77 0.03840204742484943 1102 78-79 3.108150183750187 1102 80-81 1.0881458810056195 1102 82-83 0.4805329013292159 1102 84-85 -5.2216487945767724 1102 86-87 -5.692445903035413 1102 88-89 -1.298132259126131 1102 90-91 -0.031706114421151455 1102 92-93 0.31328540054082765 1102 94-95 0.36655791904151513 1102 96-97 0.5150186281923261 1102 98-99 0.47894233215368587 1102 100 0.5811654430688087 1103 1 0.29924189840886584 1103 2 0.2863038293383333 1103 3 0.3403545315124319 1103 4 0.38341743459580613 1103 5 0.3422925583399774 1103 6 0.4362674429773321 1103 7 -11.877729743807734 1103 8 -6.323130465282794 1103 9 -2.071256982096692 1103 10-11 -0.14219048419552394 1103 12-13 0.4873126984601157 1103 14-15 0.7162524889590642 1103 16-17 0.7016323679665746 1103 18-19 0.737670257789091 1103 20-21 0.6954885462475815 1103 22-23 0.7504174707052726 1103 24-25 0.7823722740249863 1103 26-27 0.7628327831581743 1103 28-29 0.7577511470484097 1103 30-31 0.7283735229604744 1103 32-33 0.7684933839265184 1103 34-35 0.7408465013297345 1103 36-37 0.8447343614644822 1103 38-39 0.8852443896340461 1103 40-41 0.8229236351104063 1103 42-43 0.7972000579408771 1103 44-45 0.7384085691001161 1103 46-47 0.7682646765231667 1103 48-49 0.7359030670152436 1103 50-51 0.7260022894302764 1103 52-53 0.6887423694108961 1103 54-55 0.7208390021141042 1103 56-57 0.7065345129355194 1103 58-59 0.6990917896942079 1103 60-61 0.7219464990337983 1103 62-63 0.6275210761592263 1103 64-65 0.511739862943621 1103 66-67 -9.89918008785331 1103 68-69 -7.891841854128945 1103 70-71 -6.384866925668387 1103 72-73 -1.2990209449268448 1103 74-75 -0.08439431179733248 1103 76-77 0.24180986793056647 1103 78-79 3.3533882657576726 1103 80-81 1.309050873246104 1103 82-83 0.6392359842524833 1103 84-85 -5.078560049265047 1103 86-87 -5.541107322563313 1103 88-89 -1.0767371632326856 1103 90-91 0.20258141353208003 1103 92-93 0.6267762344159529 1103 94-95 0.6832873405796427 1103 96-97 0.7654137013631832 1103 98-99 0.8092226135271758 1103 100 0.9239775706803997 1104 1 0.09324474156540319 1104 2 0.1338037255234923 1104 3 0.06856421767963639 1104 4 0.15053012565991253 1104 5 0.16224726766579778 1104 6 0.24480294122428603 1104 7 -11.992989461140471 1104 8 -6.481050757827749 1104 9 -2.3609495067745883 1104 10-11 -0.3696249317097937 1104 12-13 0.25008226660933985 1104 14-15 0.47880781383839377 1104 16-17 0.46090150515246364 1104 18-19 0.4887880590729381 1104 20-21 0.44997980292993844 1104 22-23 0.477179598544879 1104 24-25 0.5266556069683546 1104 26-27 0.4893946063196566 1104 28-29 0.4909435504157713 1104 30-31 0.5065385687454551 1104 32-33 0.4893239427159699 1104 34-35 0.44961933506177587 1104 36-37 0.5353509014770381 1104 38-39 0.5749315233178365 1104 40-41 0.5767778270061896 1104 42-43 0.5113950565738961 1104 44-45 0.5007959985033921 1104 46-47 0.5500673522268897 1104 48-49 0.46116612434021675 1104 50-51 0.4703301257413628 1104 52-53 0.457615011891761 1104 54-55 0.4651970343158851 1104 56-57 0.472014122751915 1104 58-59 0.485246178068806 1104 60-61 0.4234531106164141 1104 62-63 0.309878434038815 1104 64-65 0.22025034023263146 1104 66-67 -10.042506174172875 1104 68-69 -8.059954023905526 1104 70-71 -6.561125635839058 1104 72-73 -1.7009238235596023 1104 74-75 -0.5140937989203884 1104 76-77 -0.19133134470689228 1104 78-79 2.8945958811086108 1104 80-81 0.8666748297488418 1104 82-83 0.2322086837946209 1104 84-85 -5.35046811653201 1104 86-87 -5.7863740244743305 1104 88-89 -1.423464139472049 1104 90-91 -0.11671631120492165 1104 92-93 0.24674566753210314 1104 94-95 0.2736942852761608 1104 96-97 0.38263807506458036 1104 98-99 0.37635139852674016 1104 100 0.43554270221538616 1105 1 -0.009245425162347942 1105 2 0.031617882562194666 1105 3 0.007987765751888531 1105 4 0.10198975893487017 1105 5 0.11909942026673548 1105 6 0.1588469009923088 1105 7 -12.086362294664493 1105 8 -6.592661798481043 1105 9 -2.440336208923874 1105 10-11 -0.4540143171658144 1105 12-13 0.15405538188311851 1105 14-15 0.29694006423049046 1105 16-17 0.33528589080360405 1105 18-19 0.28789141330315715 1105 20-21 0.4040636842090848 1105 22-23 0.4064808088196372 1105 24-25 0.409201942280518 1105 26-27 0.38515320877077386 1105 28-29 0.37630270900984897 1105 30-31 0.3137234549476773 1105 32-33 0.354714742117217 1105 34-35 0.43821593099654166 1105 36-37 0.48206184092173743 1105 38-39 0.42914465877424135 1105 40-41 0.4590957093427548 1105 42-43 0.3875913238277846 1105 44-45 0.30467243678788947 1105 46-47 0.3409877162477315 1105 48-49 0.303559464298516 1105 50-51 0.3890752343233217 1105 52-53 0.3484419747278338 1105 54-55 0.3564235510515772 1105 56-57 0.2948486427619912 1105 58-59 0.34345797294169245 1105 60-61 0.2567848391621581 1105 62-63 0.20637106123707838 1105 64-65 0.07662658925409005 1105 66-67 -10.154654283738193 1105 68-69 -8.255153584591106 1105 70-71 -6.759644037352427 1105 72-73 -1.8304358621666168 1105 74-75 -0.6667604005804222 1105 76-77 -0.3742911267940592 1105 78-79 2.74017008837156 1105 80-81 0.7434337533612592 1105 82-83 0.11738665422842232 1105 84-85 -5.461886162373506 1105 86-87 -5.970673065483833 1105 88-89 -1.634303068419495 1105 90-91 -0.31411173076415366 1105 92-93 0.020832739536530198 1105 94-95 0.09393014472855654 1105 96-97 0.19209251300530283 1105 98-99 0.15534407692537044 1105 100 0.23747418889162475 1106 1 -0.016381144046444973 1106 2 0.02596016256334721 1106 3 0.04020675595600309 1106 4 0.05184401530244287 1106 5 0.025877894832007087 1106 6 0.08961205715738885 1106 7 -12.08473689411663 1106 8 -6.576237061938389 1106 9 -2.5812244304193186 1106 10-11 -0.5381985617939335 1106 12-13 0.07800388108455536 1106 14-15 0.2150274951778144 1106 16-17 0.21465832525908013 1106 18-19 0.23148126493720866 1106 20-21 0.2145915735585575 1106 22-23 0.24163566896322664 1106 24-25 0.28451890625560594 1106 26-27 0.2585676152585634 1106 28-29 0.23632730427190296 1106 30-31 0.1879181643720571 1106 32-33 0.24701550203928235 1106 34-35 0.32370337739336463 1106 36-37 0.4007883663856191 1106 38-39 0.3545113939365905 1106 40-41 0.30642916415158794 1106 42-43 0.34673638572233045 1106 44-45 0.23589928612145172 1106 46-47 0.2962704306372004 1106 48-49 0.19310518120169462 1106 50-51 0.3392464121470624 1106 52-53 0.31821946940017654 1106 54-55 0.32449808238302325 1106 56-57 0.23655261676705663 1106 58-59 0.21084318517250722 1106 60-61 0.1215871041915122 1106 62-63 0.05141897308266863 1106 64-65 -0.03274848502437777 1106 66-67 -10.162102120478377 1106 68-69 -8.220916709015867 1106 70-71 -6.778218882714047 1106 72-73 -1.8577992049917427 1106 74-75 -0.7228002527732329 1106 76-77 -0.394222823251301 1106 78-79 2.7013695106419995 1106 80-81 0.65996328720124 1106 82-83 -0.008048427285974213 1106 84-85 -5.5173627945308255 1106 86-87 -5.972537882769448 1106 88-89 -1.6273555776505155 1106 90-91 -0.36700705010553847 1106 92-93 -0.014827814420435459 1106 94-95 0.0940111339237184 1106 96-97 0.14268534570046754 1106 98-99 0.15549422468750862 1106 100 0.25684271094343103 1107 1 0.14000684018150622 1107 2 0.13344547816318908 1107 3 0.10892518673307805 1107 4 0.11774527483596842 1107 5 0.11037574358364566 1107 6 0.11265503793813281 1107 7 -12.095473892915685 1107 8 -6.620950085852247 1107 9 -2.454987571940137 1107 10-11 -0.42609598051577535 1107 12-13 0.12853030577345237 1107 14-15 0.25928524295452604 1107 16-17 0.3200472508867165 1107 18-19 0.32035938446882284 1107 20-21 0.2716556534773886 1107 22-23 0.3195020702423008 1107 24-25 0.4399469440831254 1107 26-27 0.35225345959339194 1107 28-29 0.32578525815620196 1107 30-31 0.33516720511784825 1107 32-33 0.38583984545164185 1107 34-35 0.333768927971299 1107 36-37 0.39078148940076574 1107 38-39 0.39127956952955145 1107 40-41 0.3830304552703083 1107 42-43 0.3963462971278986 1107 44-45 0.37856007224727506 1107 46-47 0.39570538724178306 1107 48-49 0.40200117773427735 1107 50-51 0.4795600876089807 1107 52-53 0.41138445021150716 1107 54-55 0.33575921430274036 1107 56-57 0.3075526726148752 1107 58-59 0.27814933319924506 1107 60-61 0.266426635049676 1107 62-63 0.24891980864187246 1107 64-65 0.09457155883105628 1107 66-67 -10.077106348315898 1107 68-69 -8.136623676300239 1107 70-71 -6.622555165156168 1107 72-73 -1.7031966406926955 1107 74-75 -0.5160215871073177 1107 76-77 -0.16271722025297564 1107 78-79 2.9103302028526095 1107 80-81 0.8287133862460117 1107 82-83 0.17541361715347747 1107 84-85 -5.3953851462300015 1107 86-87 -5.860195892058556 1107 88-89 -1.5507067776154777 1107 90-91 -0.31679714522451263 1107 92-93 0.07408394165194565 1107 94-95 0.13382586967161458 1107 96-97 0.20215000620854084 1107 98-99 0.23561062592993665 1107 100 0.3070615003338304 1108 1 -0.041272493279858935 1108 2 -0.05982973686889892 1108 3 -0.012924698170216686 1108 4 -0.11288656355253579 1108 5 -0.12393967516356952 1108 6 -0.03473794604570912 1108 7 -12.102429384432735 1108 8 -6.668343357772898 1108 9 -2.730822745910828 1108 10-11 -0.676737005857774 1108 12-13 -0.11372159231319756 1108 14-15 0.08617410557342708 1108 16-17 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2216 26-27 -1.8987555592680465 2216 28-29 -2.154657210391605 2216 30-31 -1.9301391339065148 2216 32-33 -2.873695359052448 2216 34-35 -1.9155926491735897 2216 36-37 -1.7021839840194914 2216 38-39 -1.9009760104321884 2216 40-41 -1.9613069928315596 2216 42-43 -1.595811321306705 2216 44-45 -1.7290135953176176 2216 46-47 -1.8432575998591503 2216 48-49 -2.040342338050131 2216 50-51 -2.551386790478425 2216 52-53 -2.2757566477175573 2216 54-55 -1.8889714906469948 2216 56-57 -1.9263463991119067 2216 58-59 -2.203324412124445 2216 60-61 -2.344530873429541 2216 62-63 -2.2327806647687893 2216 64-65 -1.9319540201857635 2216 66-67 1.3081597351858285 2216 68-69 0.43906804835057756 2216 70-71 0.09221580303057308 2216 72-73 -1.680981343154965 2216 74-75 -2.4009516507719795 2216 76-77 -2.04422855034737 2216 78-79 0.5399643268494394 2216 80-81 -0.9503232029960778 2216 82-83 -1.844621260271527 2216 84-85 -0.07996648015052088 2216 86-87 -0.10530432840451098 2216 88-89 -1.2930451692828129 2216 90-91 -1.5489634227345483 2216 92-93 -1.6516715718772588 2216 94-95 -1.638068901040807 2216 96-97 -1.639402230579826 2216 98-99 -1.5960345542915206 2216 100 -2.6125832746264415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138448.0 3 24379.0 4 14781.0 5 13137.0 6 12147.0 7 13142.0 8 14902.0 9 16822.0 10 18781.0 11 20861.0 12 22973.0 13 25302.0 14 28875.0 15 31324.0 16 33308.0 17 33589.0 18 32007.0 19 30417.0 20 29110.0 21 29393.0 22 31019.0 23 35759.0 24 43511.0 25 54860.0 26 68172.0 27 83037.0 28 101854.0 29 126786.0 30 154535.0 31 190306.0 32 251040.0 33 348280.0 34 512723.0 35 670418.0 36 785343.0 37 1255863.0 38 1305172.0 39 378420.0 40 666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83109862880563 20.509106339768305 14.655628383861075 23.004166647564993 2 15.318136873562144 20.244311998921695 40.696177899906886 23.74137322760928 3 18.49544445628546 25.1511877874072 30.33056196307208 26.022805793235264 4 12.87562543661137 17.11798752184937 38.32421161433155 31.68217542720771 5 13.364407588111282 37.71221922661861 35.245644748531156 13.677728436738953 6 30.86913580600595 37.529237263553995 17.88875766428481 13.712869266155248 7 27.21392608518406 32.60475982748189 22.293855925051737 17.887458162282307 8 24.770742861595465 36.550940189891456 20.511104980590027 18.16721196792305 9 24.726096052660605 17.626637515179485 21.67246631149751 35.9748001206624 10-11 23.798288954376602 27.31044013417247 28.77164553785439 20.119625373596534 12-13 24.10388397158074 24.791311619256824 28.872591442881163 22.232212966281274 14-15 21.624632491016925 27.10427271537108 25.96587935306387 25.305215440548125 16-17 21.322661617855502 30.349237847663495 26.705708724647355 21.62239180983365 18-19 21.283829948512217 29.43177231359277 28.44882633465598 20.835571403239033 20-21 22.282103825968242 28.607169143595506 28.03795451711651 21.07277251331974 22-23 22.087479671163432 28.40804691051817 28.04348859880638 21.460984819512017 24-25 21.872424429152517 28.491260140068082 28.04934697059155 21.58696846018785 26-27 21.65186721213112 28.520203035157017 28.04338954753102 21.784540205180843 28-29 21.64427021159751 28.577724837577 28.211741322949262 21.566263627876225 30-31 21.91747946203818 28.76651767208645 27.86727908853475 21.44872377734062 32-33 21.594206541740085 28.651297246471565 28.150241629240757 21.60425458254759 34-35 21.790391981046945 28.42202060886978 28.1177862510134 21.66980115906988 36-37 22.012659243195547 28.48513061737803 27.880149710805473 21.622060428620948 38-39 21.831092636783712 28.60679571321758 27.988314765106438 21.57379688489227 40-41 21.75368855405931 28.482687437101283 28.08264228896469 21.68098171987472 42-43 21.687897539490482 28.49543444383879 28.044626192470755 21.772041824199963 44-45 21.793742520808884 28.59470452678658 27.940299251438116 21.671253700966425 46-47 21.781346942824168 28.641451341617962 27.87293813814631 21.70426357741156 48-49 21.741191805397893 28.478949498132543 27.969798429403742 21.81006026706582 50-51 21.713992978956135 28.474073509614527 27.927087540171687 21.88484597125765 52-53 21.700726468413563 28.522629839147935 27.946910998094094 21.829732694344408 54-55 21.76754117364208 28.65253342846177 27.836347767011056 21.743577630885095 56-57 21.67563650572904 28.48902341220384 27.865477194339956 21.96986288772716 58-59 21.643701974463156 28.291390231416376 28.055142476296446 22.009765317824026 60-61 21.780768811797834 28.531579736308714 27.794927753216825 21.89272369867663 62-63 21.610706549993974 28.60016760497137 27.867717693984208 21.921408151050453 64-65 21.66055542210559 28.389685267682253 27.930249736692947 22.019509573519212 66-67 21.56812052010374 28.35904009198298 27.897527417199075 22.175311970714205 68-69 21.925963933129104 28.438494268014107 27.708213648538244 21.927328150318544 70-71 21.681985118358917 28.26133120306694 27.892896244839687 22.163787433734456 72-73 21.570174983124772 28.365063535006474 28.01048502178113 22.054276460087628 74-75 21.53276960599295 28.382016684354465 27.942361784838322 22.142851924814263 76-77 21.576884976447978 28.359496238058092 27.81345976538867 22.250159020105258 78-79 21.666237998750827 28.296076104746938 27.96912651321416 22.068559383288076 80-81 21.53226503609491 28.251354602015628 28.03194821682714 22.184432145062328 82-83 21.482177697252727 28.20731725746554 28.12890584349102 22.181599201790714 84-85 21.55652407525014 28.12890146173974 27.96068661846319 22.353887844546932 86-87 21.59164556071756 28.206696325649013 27.948311236395963 22.25334687723747 88-89 21.467689847516947 28.0612418830355 28.064013546919043 22.407054722528514 90-91 21.415689445756332 27.94623214561837 28.093247691364613 22.54483071726068 92-93 21.50199145234319 28.03478307454229 28.018761500063423 22.444463973051096 94-95 21.466772131273643 27.823425948619235 28.134605746054426 22.575196174052696 96-97 21.408833902977154 27.859357253837878 28.12120815388276 22.610600689302206 98-99 21.40730433023824 27.84041868440464 28.108635516251688 22.64364146910543 100 21.459882768183537 27.758557464217365 28.066921261015548 22.714638506583544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2506.0 1 1972.5 2 1655.0 3 1863.0 4 1705.0 5 1446.5 6 1339.0 7 1356.5 8 1432.5 9 1470.0 10 1344.0 11 1219.0 12 1208.0 13 1280.5 14 1423.0 15 1573.5 16 1850.5 17 2356.5 18 3134.5 19 4288.0 20 6136.5 21 8915.5 22 13196.0 23 19487.5 24 27507.5 25 37259.5 26 50238.0 27 66469.0 28 86041.0 29 106739.5 30 124689.5 31 142822.5 32 161126.5 33 177404.0 34 193032.5 35 208692.5 36 222650.0 37 234426.5 38 245596.5 39 251865.0 40 254549.0 41 255670.0 42 251984.5 43 248655.0 44 245499.5 45 240365.5 46 235098.0 47 227877.5 48 220192.5 49 215843.0 50 210862.0 51 202463.5 52 192910.5 53 184656.0 54 177702.5 55 167800.0 56 154146.5 57 138268.5 58 123521.5 59 110863.5 60 96803.5 61 82021.5 62 68889.5 63 56733.5 64 45686.5 65 36359.5 66 28664.5 67 22219.5 68 17069.0 69 13121.0 70 9772.0 71 7188.5 72 5495.5 73 3941.5 74 2732.0 75 1979.5 76 1282.5 77 804.5 78 541.0 79 419.5 80 308.0 81 186.0 82 134.0 83 108.5 84 91.5 85 72.0 86 59.5 87 48.0 88 39.0 89 36.0 90 31.5 91 25.0 92 15.5 93 18.0 94 21.5 95 14.5 96 15.0 97 14.0 98 15.0 99 26.0 100 39.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018191032193543413 2 0.0020482815776981956 3 0.0011172444969262884 4 0.001990986988112232 5 0.0017188376875789052 6 0.00310823148503852 7 11.73905981297327 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3634018204209947E-4 18-19 0.0 20-21 7.161823698245439E-6 22-23 0.0 24-25 0.0 26-27 1.5756012136139966E-4 28-29 0.0 30-31 0.0 32-33 2.148547109473632E-5 34-35 2.8647294792981755E-5 36-37 2.8647294792981755E-5 38-39 2.8647294792981755E-5 40-41 0.0 42-43 2.148547109473632E-5 44-45 1.2175100287017246E-4 46-47 8.522570200912073E-4 48-49 5.299749536701625E-4 50-51 3.079584190245539E-4 52-53 2.2917835834385404E-4 54-55 3.3660571381753565E-4 56-57 0.0014753356818385606 58-59 2.8647294792981756E-4 60-61 0.001124406320624534 62-63 0.00237056364411924 64-65 9.883316703578706E-4 66-67 5.869995425027021 68-69 5.504176632344343 70-71 0.0025138001180841493 72-73 0.0010671117310385705 74-75 0.0027358166527297577 76-77 0.006109035614603359 78-79 5.493161747496441 80-81 0.005049085707263035 82-83 0.004805583701522689 84-85 5.869923806790039 86-87 0.0030867460139437845 88-89 0.0013750701500631244 90-91 0.0032586297827016746 92-93 0.003588073672820965 94-95 0.004139534097585864 96-97 0.002850405831901685 98-99 0.005106380296848998 100 0.006044579201319151 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6981462.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.63008640666231 #Duplication Level Percentage of deduplicated Percentage of total 1 90.98970906769793 78.82446358651737 2 6.3611125924707785 11.021274670565024 3 1.3955031250261256 3.626776689053416 4 0.5457657550477233 1.8911893807032625 5 0.26611631301367533 1.1526839595298544 6 0.1423256824116636 0.7397811699125757 7 0.08445523276888223 0.5121454878584133 8 0.05916651034993004 0.4100479923196096 9 0.0395686195887589 0.3085049640569881 >10 0.11541365974843787 1.4558411050057902 >50 8.02907661795634E-4 0.045725964864339774 >100 4.324261658648935E-5 0.003970308505699626 >500 1.729159772524789E-5 0.007594721107590675 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.4323647396490878E-5 0.0 0.0 1.4323647396490878E-5 3 0.0 1.4323647396490878E-5 0.0 0.0 1.4323647396490878E-5 4 0.0 1.4323647396490878E-5 0.0 0.0 1.4323647396490878E-5 5 0.0 2.8647294792981755E-5 0.0 1.4323647396490878E-5 1.4323647396490878E-5 6 0.0 2.8647294792981755E-5 0.0 1.4323647396490878E-5 1.4323647396490878E-5 7 0.0 2.8647294792981755E-5 0.0 1.4323647396490878E-5 1.4323647396490878E-5 8 0.0 2.8647294792981755E-5 0.0 1.4323647396490878E-5 1.4323647396490878E-5 9 0.0 2.8647294792981755E-5 0.0 7.161823698245439E-5 1.4323647396490878E-5 10-11 0.0 3.5809118491227195E-5 0.0 7.878006068069983E-5 1.4323647396490878E-5 12-13 0.0 5.0132765887718074E-5 0.0 1.0026553177543615E-4 1.4323647396490878E-5 14-15 0.0 7.878006068069983E-5 0.0 1.0742735547368158E-4 1.4323647396490878E-5 16-17 0.0 1.0742735547368158E-4 0.0 1.2175100287017246E-4 1.4323647396490878E-5 18-19 0.0 1.503982976631542E-4 0.0 1.289128265684179E-4 1.4323647396490878E-5 20-21 0.0 1.5756012136139966E-4 0.0 1.3607465026666335E-4 1.4323647396490878E-5 22-23 0.0 1.5756012136139966E-4 0.0 2.0769288724911775E-4 1.4323647396490878E-5 24-25 0.0 1.7188376875789052E-4 0.0 4.3687124559297177E-4 1.4323647396490878E-5 26-27 0.0 2.005310635508723E-4 0.0 8.594188437894526E-4 1.4323647396490878E-5 28-29 0.0 2.005310635508723E-4 0.0 0.002549609236575376 1.4323647396490878E-5 30-31 0.0 2.005310635508723E-4 0.0 0.007548562177950692 1.4323647396490878E-5 32-33 0.0 2.1485471094736318E-4 0.0 0.019680691522778467 1.4323647396490878E-5 34-35 0.0 2.2917835834385407E-4 0.0 0.039031939155437644 1.4323647396490878E-5 36-37 0.0 2.578256531368358E-4 0.0 0.07014290130061583 1.4323647396490878E-5 38-39 0.0 2.93634771628063E-4 0.0 0.11332869820103583 1.4323647396490878E-5 40-41 0.0 3.509293612140265E-4 0.0 0.15965853570498556 1.4323647396490878E-5 42-43 0.0 3.5809118491227196E-4 0.0 0.20745654706707561 1.4323647396490878E-5 44-45 0.0 3.5809118491227196E-4 0.0 0.2693848365858039 1.4323647396490878E-5 46-47 0.0 3.5809118491227196E-4 0.0 0.3467683416453459 1.4323647396490878E-5 48-49 0.0 3.724148323087628E-4 0.0 0.4285850155741018 1.4323647396490878E-5 50-51 0.0 3.724148323087628E-4 0.0 0.5137176138751454 1.4323647396490878E-5 52-53 0.0 3.724148323087628E-4 0.0 0.6006191826296554 1.4323647396490878E-5 54-55 0.0 3.724148323087628E-4 0.0 0.696458707359576 1.4323647396490878E-5 56-57 0.0 3.724148323087628E-4 0.0 0.805125058333054 1.4323647396490878E-5 58-59 0.0 4.010621271017446E-4 0.0 0.9246272485619774 1.4323647396490878E-5 60-61 0.0 4.010621271017446E-4 0.0 1.050675345651097 1.4323647396490878E-5 62-63 0.0 4.010621271017446E-4 0.0 1.1805765038898728 1.4323647396490878E-5 64-65 0.0 4.010621271017446E-4 0.0 1.3241567453922976 1.4323647396490878E-5 66-67 0.0 4.010621271017446E-4 0.0 1.4915930216335775 1.4323647396490878E-5 68-69 0.0 4.010621271017446E-4 0.0 1.6574465348375456 1.4323647396490878E-5 70-71 0.0 4.1538577449823545E-4 0.0 1.8622961780784597 1.4323647396490878E-5 72-73 0.0 4.7268036408419894E-4 0.0 2.0851005133308753 1.4323647396490878E-5 74-75 0.0 4.941658351789353E-4 0.0 2.291260770308569 1.4323647396490878E-5 76-77 0.0 5.156513062736716E-4 0.0 2.51085660854417 1.4323647396490878E-5 78-79 0.0 5.156513062736716E-4 0.0 2.769405605874529 1.4323647396490878E-5 80-81 0.0 5.299749536701625E-4 0.0 3.0833298240397213 1.4323647396490878E-5 82-83 0.0 5.299749536701625E-4 0.0 3.410517739694064 1.4323647396490878E-5 84-85 0.0 5.442986010666534E-4 0.0 3.7427977119978593 1.4323647396490878E-5 86-87 0.0 5.514604247648987E-4 0.0 4.138359558499351 1.4323647396490878E-5 88 0.0 5.729458958596351E-4 0.0 4.473704791345996 1.4323647396490878E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6905 0.0 44.22837 1 GTATCAA 10800 0.0 36.24833 1 TCAACGC 11490 0.0 33.083973 4 ATCAACG 11600 0.0 32.72608 3 CAACGCA 12350 0.0 30.863115 5 TATCAAC 13280 0.0 28.701767 2 AACGCAG 13530 0.0 28.246931 6 GTACATG 19870 0.0 24.06036 1 ACGCAGA 16095 0.0 23.076515 7 TACATGG 20535 0.0 22.50327 2 CGCAGAG 16300 0.0 22.136787 8 ACATGGG 20860 0.0 21.465 3 CATGGGG 14480 0.0 18.22101 4 GCAGAGT 18750 0.0 18.134928 9 GAGTACA 15305 0.0 17.878302 1 GTGGTAT 4250 0.0 17.723387 1 CAGAGTA 17310 0.0 16.234665 10-11 AGAGTAC 15660 0.0 15.649728 10-11 GTATAGA 2850 0.0 15.642017 1 AGTACAT 16390 0.0 15.597513 2 >>END_MODULE