FastQCFastQC Report
Wed 25 May 2016
SRR2049497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6981462
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196130.2809296963873756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196040.2808007835608072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115230.1650513889497644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128200.055.853391
GTATCAA222400.038.3981971
ATCAACG276500.030.3428133
TCAACGC279050.029.9980894
GTGGTAT53850.029.9858781
TATCAAC288150.029.1323742
CAACGCA289450.028.918345
AACGCAG304350.027.6102926
TGGTATC60750.024.7858222
ACGCAGA348450.023.9402377
CGCAGAG349800.023.6597468
GTACATG202250.022.3688261
GCAGAGT381100.021.3465989
GAGTACT249600.021.09783212-13
TACATGG206650.021.039542
CAGAGTA365350.020.0414410-11
ACATGGG208100.019.8528143
AGAGTAC349800.019.77691510-11
AGTACTT266050.018.8129512-13
GTACTTT282900.018.39014814-15
Produced by FastQC (version 0.11.5)