##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049497_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6981462
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74996225718911	31.0	31.0	34.0	30.0	34.0
2	31.79483366091515	31.0	31.0	34.0	30.0	34.0
3	31.86481198350718	33.0	31.0	34.0	30.0	34.0
4	35.57787108201692	37.0	35.0	37.0	33.0	37.0
5	35.72926515964708	37.0	35.0	37.0	33.0	37.0
6	35.824271048098524	37.0	35.0	37.0	35.0	37.0
7	35.797774162489176	37.0	35.0	37.0	35.0	37.0
8	35.79529130144947	37.0	35.0	37.0	33.0	37.0
9	37.527388102950354	39.0	37.0	39.0	35.0	39.0
10-11	37.49371091613762	39.0	37.0	39.0	34.5	39.0
12-13	37.49120169958671	39.0	37.0	39.0	34.5	39.0
14-15	38.77414959789225	40.0	38.0	41.0	35.0	41.0
16-17	38.78534145140373	40.0	38.0	41.0	35.0	41.0
18-19	38.849009204662295	40.0	38.0	41.0	35.0	41.0
20-21	38.8750788158698	40.0	38.0	41.0	35.0	41.0
22-23	38.85742456236244	40.0	38.0	41.0	35.0	41.0
24-25	38.830322860741774	40.0	38.0	41.0	35.0	41.0
26-27	38.74504802862208	40.0	38.0	41.0	35.0	41.0
28-29	38.66307458237257	40.0	38.0	41.0	34.0	41.0
30-31	38.556416406764086	40.0	38.0	41.0	34.0	41.0
32-33	38.428202860661564	40.0	38.0	41.0	34.0	41.0
34-35	38.33769194761785	40.0	38.0	41.0	34.0	41.0
36-37	38.214799779759595	40.0	38.0	41.0	34.0	41.0
38-39	38.13516982546063	40.0	38.0	41.0	34.0	41.0
40-41	38.02266652744082	40.0	38.0	41.0	33.0	41.0
42-43	37.88998085214816	40.0	37.0	41.0	33.0	41.0
44-45	37.7192176223261	40.0	37.0	41.0	33.0	41.0
46-47	37.490494469496504	40.0	37.0	41.0	33.0	41.0
48-49	37.26598683771394	40.0	37.0	41.0	32.0	41.0
50-51	37.05968671031941	40.0	37.0	41.0	31.0	41.0
52-53	36.963771771585954	40.0	36.0	41.0	31.0	41.0
54-55	36.842310178011424	40.0	36.0	41.0	31.0	41.0
56-57	36.674405661736756	39.0	36.0	41.0	31.0	41.0
58-59	36.506307919458706	39.0	35.0	41.0	31.0	41.0
60-61	36.320078086796144	39.0	35.0	41.0	31.0	41.0
62-63	36.119012249869726	39.0	35.0	41.0	30.5	41.0
64-65	35.853377057699376	38.0	35.0	40.0	30.0	41.0
66-67	35.59808167687513	38.0	35.0	40.0	30.0	41.0
68-69	35.28897865518712	37.0	35.0	40.0	29.0	41.0
70-71	34.99916092073551	37.0	34.5	39.5	29.0	41.0
72-73	34.68027298580154	36.0	34.0	39.0	29.0	41.0
74-75	34.334033759691025	36.0	34.0	39.0	29.0	40.5
76-77	34.04117239340413	35.0	34.0	38.5	29.0	40.0
78-79	33.71369077135992	35.0	34.0	37.0	29.0	39.0
80-81	33.404605940704116	35.0	34.0	37.0	29.0	39.0
82-83	33.11169952654616	35.0	34.0	36.0	28.0	39.0
84-85	32.83982280502279	35.0	34.0	36.0	28.0	37.0
86-87	32.57535749675355	35.0	33.0	36.0	27.0	37.0
88-89	32.36496080620363	35.0	33.0	35.0	27.0	37.0
90-91	32.21512342257252	35.0	33.0	35.0	27.0	36.0
92-93	32.06316341763373	35.0	33.0	35.0	27.0	36.0
94-95	31.88782485674204	35.0	33.0	35.0	26.0	36.0
96-97	31.72839900868901	35.0	33.0	35.0	26.0	36.0
98-99	31.557335053889858	35.0	33.0	35.0	25.5	35.0
100	31.452684409082224	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31490667628700564
1101	2	0.39974643658933573
1101	3	0.48181850087904365
1101	4	0.384775054491314
1101	5	0.2562608927319374
1101	6	0.28386507891903534
1101	7	0.2844451759095534
1101	8	0.3039668230502812
1101	9	0.32158442104933016
1101	10-11	0.3364974851951317
1101	12-13	0.3139899933644159
1101	14-15	0.47060575903182666
1101	16-17	0.4514293975315766
1101	18-19	0.45916762365839503
1101	20-21	0.4244986477292869
1101	22-23	0.41567632685814715
1101	24-25	0.4565324990921269
1101	26-27	0.47424551021514105
1101	28-29	0.4426748414036794
1101	30-31	0.4660220472538583
1101	32-33	0.5101362493124668
1101	34-35	0.5206332334953032
1101	36-37	0.5216208694642006
1101	38-39	0.5121895831160828
1101	40-41	0.586170589115504
1101	42-43	0.4377441102166486
1101	44-45	0.5002780862591862
1101	46-47	0.5842751372952506
1101	48-49	0.6246093064496847
1101	50-51	0.64062823526411
1101	52-53	0.6043962778021523
1101	54-55	0.5924477072260927
1101	56-57	0.5596031923685985
1101	58-59	0.5948445040259713
1101	60-61	0.6579524308201599
1101	62-63	0.5587447163275101
1101	64-65	0.6134084488829856
1101	66-67	0.5816965794548352
1101	68-69	0.5126507686005013
1101	70-71	0.5342065914617322
1101	72-73	0.48016180202781555
1101	74-75	0.49063534857839386
1101	76-77	0.4773641688746082
1101	78-79	0.4657240565354215
1101	80-81	0.4804614507090932
1101	82-83	0.48575692015631944
1101	84-85	0.4912506940043144
1101	86-87	0.5277474694718549
1101	88-89	0.5319800101850589
1101	90-91	0.5291408642911506
1101	92-93	0.5002795809929808
1101	94-95	0.4637204384630067
1101	96-97	0.5106708004457268
1101	98-99	0.5723337032211226
1101	100	0.5901146043882797
1102	1	0.22420352466648552
1102	2	0.32147276520127477
1102	3	0.40091437691454246
1102	4	0.31184960415467344
1102	5	0.24466124271030765
1102	6	0.27805609815345633
1102	7	0.26584407742589633
1102	8	0.3142244942983936
1102	9	0.36328326452749593
1102	10-11	0.3230726281720919
1102	12-13	0.32783842278333
1102	14-15	0.4109595389007765
1102	16-17	0.40429697334070624
1102	18-19	0.40354328629850045
1102	20-21	0.3868535981428991
1102	22-23	0.37941333539703237
1102	24-25	0.3539853982122594
1102	26-27	0.37460095758826384
1102	28-29	0.40430939333176497
1102	30-31	0.45093617078855175
1102	32-33	0.43364554712955794
1102	34-35	0.45826855832521574
1102	36-37	0.5045414414341209
1102	38-39	0.49606045377428387
1102	40-41	0.5291649930542377
1102	42-43	0.5254241886768156
1102	44-45	0.5671471952692855
1102	46-47	0.6028531888584681
1102	48-49	0.6168678642118692
1102	50-51	0.666235912630178
1102	52-53	0.6563564369608628
1102	54-55	0.608032814504547
1102	56-57	0.6061124656386809
1102	58-59	0.5514082961242224
1102	60-61	0.5812268437914412
1102	62-63	0.5239444213494977
1102	64-65	0.5365173528944638
1102	66-67	0.5876074632536827
1102	68-69	0.5909169159990313
1102	70-71	0.5643175411519223
1102	72-73	0.5679858661352242
1102	74-75	0.5316521188927155
1102	76-77	0.5057774102685499
1102	78-79	0.4671200951737404
1102	80-81	0.45256438321223413
1102	82-83	0.47895213937574255
1102	84-85	0.4820181992478254
1102	86-87	0.5219916692048088
1102	88-89	0.48436449413860316
1102	90-91	0.48638390335466397
1102	92-93	0.5222511070188816
1102	94-95	0.5109871517596005
1102	96-97	0.5469816538852719
1102	98-99	0.5404406683945417
1102	100	0.628078530056154
1103	1	0.32289171063210276
1103	2	0.3299245957276007
1103	3	0.4386268296682019
1103	4	0.3622349152324915
1103	5	0.27776971229159386
1103	6	0.29099917920903806
1103	7	0.32190224108761356
1103	8	0.34436817972711964
1103	9	0.3716321831900018
1103	10-11	0.32420559857123266
1103	12-13	0.32240143395786447
1103	14-15	0.4490672711441803
1103	16-17	0.43978032468064043
1103	18-19	0.4677540355641838
1103	20-21	0.4630470598660068
1103	22-23	0.4527905488159121
1103	24-25	0.45569523898707587
1103	26-27	0.4566250095662525
1103	28-29	0.44763699356739295
1103	30-31	0.4680736369955767
1103	32-33	0.45211701628018375
1103	34-35	0.4706932883789534
1103	36-37	0.5252011791971256
1103	38-39	0.5527179279714503
1103	40-41	0.5360193193165941
1103	42-43	0.5448698956400548
1103	44-45	0.5587923162832027
1103	46-47	0.5818133566762427
1103	48-49	0.5821907520873069
1103	50-51	0.6530901484790022
1103	52-53	0.6069672014042737
1103	54-55	0.5268620837133042
1103	56-57	0.5715642798979061
1103	58-59	0.5678952755406215
1103	60-61	0.5802950022188966
1103	62-63	0.5819550448835287
1103	64-65	0.5591401599057306
1103	66-67	0.5848770573635349
1103	68-69	0.6026357465923482
1103	70-71	0.6072848489971889
1103	72-73	0.6284607350177325
1103	74-75	0.6731841010445407
1103	76-77	0.6775325656412576
1103	78-79	0.5863953121244734
1103	80-81	0.5716343220341926
1103	82-83	0.5922241184219601
1103	84-85	0.6135487905352193
1103	86-87	0.6395692484555155
1103	88-89	0.6354193815479903
1103	90-91	0.6230659564590937
1103	92-93	0.6426820857291418
1103	94-95	0.6355013005682579
1103	96-97	0.6089181591634407
1103	98-99	0.6406547797228619
1103	100	0.629681325253511
1104	1	0.2136003981743002
1104	2	0.21803006022471294
1104	3	0.3208123186508658
1104	4	0.23827019162133212
1104	5	0.21289903185855508
1104	6	0.2163265332290294
1104	7	0.18182002605619374
1104	8	0.18203391195480378
1104	9	0.25211948268243844
1104	10-11	0.26374204263991174
1104	12-13	0.2641708983976514
1104	14-15	0.34256718145237386
1104	16-17	0.30443762608916813
1104	18-19	0.30757777271722375
1104	20-21	0.31876924966146447
1104	22-23	0.312063496059217
1104	24-25	0.3023735750743981
1104	26-27	0.3526831652708111
1104	28-29	0.32800883198385833
1104	30-31	0.353681643111905
1104	32-33	0.4008622584512267
1104	34-35	0.3967245281094449
1104	36-37	0.38412442324548124
1104	38-39	0.384123467109859
1104	40-41	0.4139630234259499
1104	42-43	0.432287204746693
1104	44-45	0.4686297317004602
1104	46-47	0.49885199139617953
1104	48-49	0.43688095911201685
1104	50-51	0.5159114027182881
1104	52-53	0.487991861276285
1104	54-55	0.406342721770919
1104	56-57	0.41816804847153577
1104	58-59	0.43916007547940694
1104	60-61	0.4625616986493313
1104	62-63	0.44235346095359773
1104	64-65	0.4641937399579419
1104	66-67	0.44558372973843774
1104	68-69	0.42144930946925996
1104	70-71	0.47263720921972663
1104	72-73	0.5271251702449646
1104	74-75	0.5010107684110352
1104	76-77	0.41058524055451073
1104	78-79	0.4480251988261159
1104	80-81	0.461409967093239
1104	82-83	0.4031764209297748
1104	84-85	0.4847913519646241
1104	86-87	0.4610305904431158
1104	88-89	0.45022887973128434
1104	90-91	0.489783560905785
1104	92-93	0.52986849903278
1104	94-95	0.4960047171832329
1104	96-97	0.5183917483513447
1104	98-99	0.5996136633539102
1104	100	0.5865621203086739
1105	1	0.2558612310763806
1105	2	0.3065191939787191
1105	3	0.3650390163454631
1105	4	0.30214150171794785
1105	5	0.2291137546791333
1105	6	0.25989526801348006
1105	7	0.2524093081333234
1105	8	0.24530284083194687
1105	9	0.257485739532207
1105	10-11	0.2556563331256143
1105	12-13	0.2535190825881344
1105	14-15	0.38681411730153314
1105	16-17	0.3075432339506108
1105	18-19	0.32079168285299176
1105	20-21	0.3105417329647935
1105	22-23	0.3474898570439038
1105	24-25	0.37487782725204966
1105	26-27	0.3276579802948376
1105	28-29	0.34980982039036945
1105	30-31	0.36356025697892136
1105	32-33	0.3337157042562211
1105	34-35	0.28160514159273475
1105	36-37	0.34719045347886635
1105	38-39	0.3637912923822171
1105	40-41	0.35580586520214297
1105	42-43	0.371528634900784
1105	44-45	0.4061545231213728
1105	46-47	0.3749553720162382
1105	48-49	0.3269368342495582
1105	50-51	0.36297569346381664
1105	52-53	0.27321024943170613
1105	54-55	0.25205402374476904
1105	56-57	0.3103532729527885
1105	58-59	0.31042546730281373
1105	60-61	0.2783800491253601
1105	62-63	0.2953112449528561
1105	64-65	0.3108733784028601
1105	66-67	0.36220826436277065
1105	68-69	0.3638270040222409
1105	70-71	0.380473774187692
1105	72-73	0.3390606734752737
1105	74-75	0.3246473383309194
1105	76-77	0.3169603025426184
1105	78-79	0.32295044883929336
1105	80-81	0.2830346301005875
1105	82-83	0.2908480784348839
1105	84-85	0.3179538645749389
1105	86-87	0.33706635346560887
1105	88-89	0.31131823705512573
1105	90-91	0.27907128281351845
1105	92-93	0.2797336968755886
1105	94-95	0.30103871677342653
1105	96-97	0.2986064724965125
1105	98-99	0.36473491448095174
1105	100	0.3663827672102684
1106	1	0.10522945852397214
1106	2	0.11007519707382585
1106	3	0.2626170951106843
1106	4	0.2203866176824576
1106	5	0.12420079761336922
1106	6	0.1755159175768597
1106	7	0.15909617414574484
1106	8	0.1566983549348393
1106	9	0.141805218362002
1106	10-11	0.20479702673079458
1106	12-13	0.18452952932319278
1106	14-15	0.21477891731658616
1106	16-17	0.21089354340574573
1106	18-19	0.25873609312052537
1106	20-21	0.20367855574772165
1106	22-23	0.18913840463891773
1106	24-25	0.21367129416013597
1106	26-27	0.22162578441002978
1106	28-29	0.2595264887092483
1106	30-31	0.2962296382023055
1106	32-33	0.309386195774934
1106	34-35	0.2711560088843896
1106	36-37	0.32054311254984924
1106	38-39	0.3024809865950715
1106	40-41	0.32477773225870976
1106	42-43	0.34451798749305595
1106	44-45	0.3405390923915519
1106	46-47	0.284030008902441
1106	48-49	0.2853911401116278
1106	50-51	0.31480609200076515
1106	52-53	0.3227550120860343
1106	54-55	0.24366363189735551
1106	56-57	0.27531886702201547
1106	58-59	0.30532409379425474
1106	60-61	0.3028754269959961
1106	62-63	0.3155282536843771
1106	64-65	0.3031909575623146
1106	66-67	0.2999580043814021
1106	68-69	0.2562276688703591
1106	70-71	0.2904470078477388
1106	72-73	0.27281629884149083
1106	74-75	0.305182906056757
1106	76-77	0.27784801347925736
1106	78-79	0.3154151143991726
1106	80-81	0.2609833969596167
1106	82-83	0.2223862201353981
1106	84-85	0.2541325411419635
1106	86-87	0.3092721716839293
1106	88-89	0.2983837598278143
1106	90-91	0.26908360267882614
1106	92-93	0.3115851494506501
1106	94-95	0.2788063198791164
1106	96-97	0.28501870980626975
1106	98-99	0.3422386929189365
1106	100	0.31807827086720053
1107	1	0.19530071228916057
1107	2	0.1680474025173666
1107	3	0.27263476624510474
1107	4	0.26731629818328173
1107	5	0.20681596100992294
1107	6	0.20349612038178577
1107	7	0.2025340144454617
1107	8	0.20744280434371376
1107	9	0.2137806809595162
1107	10-11	0.17753797979613495
1107	12-13	0.17613881055562786
1107	14-15	0.2214967342725913
1107	16-17	0.20401992586919704
1107	18-19	0.23775846760509012
1107	20-21	0.2630083280676274
1107	22-23	0.2840171633340276
1107	24-25	0.2682284277525895
1107	26-27	0.2811664274247292
1107	28-29	0.2771430741973475
1107	30-31	0.29587295080278864
1107	32-33	0.31200631169506465
1107	34-35	0.27404339299671676
1107	36-37	0.25863215816803375
1107	38-39	0.2653312127738303
1107	40-41	0.2610360314776159
1107	42-43	0.3119804751970463
1107	44-45	0.2932318691030247
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2216	96-97	-1.7832113632593654
2216	98-99	-1.7680698548424267
2216	100	-1.8274366447833899
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	364.0
3	27.0
4	66.0
5	114.0
6	331.0
7	909.0
8	1961.0
9	3181.0
10	4869.0
11	6906.0
12	9679.0
13	14196.0
14	16823.0
15	22089.0
16	29217.0
17	42077.0
18	42293.0
19	22919.0
20	16956.0
21	16718.0
22	17769.0
23	19412.0
24	22294.0
25	27997.0
26	36066.0
27	47154.0
28	62038.0
29	82585.0
30	107955.0
31	140727.0
32	182368.0
33	237593.0
34	322554.0
35	470222.0
36	770510.0
37	1410725.0
38	1983220.0
39	786079.0
40	2499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.127032951157574	20.770305003942717	15.103351984931962	22.999310059967744
2	15.558085118207638	20.589680301095303	39.9727620818845	23.879472498812568
3	18.02628418321036	25.381334938346	30.870973854899425	25.721407023544213
4	12.920859438368407	17.574233713727814	37.700627782832115	31.80427906507166
5	12.861957733042653	37.61923928644072	35.365846671269416	14.152956309247223
6	30.476615689558884	37.961594638530066	17.400011373127533	14.16177829878352
7	26.52229575982796	33.1240648448706	22.2610966012563	18.092542794045144
8	24.88452991651319	36.47479568033171	20.095461380438653	18.545213022716446
9	24.723657332944303	17.024206544792428	21.706852392888077	36.54528372937519
10-11	23.86757243683343	27.20571994805672	28.45367488929969	20.473032725810153
12-13	24.587378689449288	24.634002161724865	28.990367633598808	21.78825151522704
14-15	21.7604269021093	26.637981167263007	26.795090138738885	24.806501791888802
16-17	20.760830611124145	29.934740030096847	27.584802438228557	21.719626920550454
18-19	20.672131424621377	28.236098685346995	30.46504442765713	20.6267254623745
20-21	21.60715047936951	27.36423259196999	30.403775025918637	20.62484190274186
22-23	21.366742381466803	27.189670301148954	30.49365591333162	20.94993140405262
24-25	21.21287059931	27.29933930744019	30.42369921944716	21.06409087380265
26-27	21.057860087185176	27.328144162354533	30.42186579258041	21.19212995787988
28-29	21.049271548487113	27.333994800222822	30.59522136724261	21.02151228404746
30-31	21.315112453145417	27.517334888313172	30.225608151669547	20.941944506871863
32-33	21.028906340026367	27.416250880993903	30.513926059751245	21.040916719228488
34-35	21.221271418508042	27.26047925205351	30.386207072386846	21.1320422570516
36-37	21.443438351451316	27.34125746154602	30.095623237654234	21.11968094934843
38-39	21.29108487591854	27.48114220202015	30.161139600845782	21.066633321215527
40-41	21.25675825493285	27.373915377610018	30.152352043168033	21.216974324289094
42-43	21.276145311684	27.408736164430884	30.00824898853564	21.306869535349474
44-45	21.39889180804823	27.525244712353945	29.783697168300847	21.292166311296974
46-47	21.466836029473484	27.677197125759616	29.441741572180725	21.414225272586172
48-49	21.563434707515416	27.687588931945772	29.15304845890445	21.595927901634358
50-51	21.61762106561634	27.79417835404676	28.938659266497474	21.64954131383942
52-53	21.586989945658946	27.91373783886527	28.88931430121656	21.60995791425922
54-55	21.604436148187872	28.05214008183386	28.7932455981283	21.550178171849964
56-57	21.479591237480058	27.86646264063315	28.867750050061147	21.786196071825646
58-59	21.457972824258352	27.621222362642012	29.10107317293965	21.819731640159983
60-61	21.567574241613002	27.789795317943433	28.92342606749131	21.719204372952255
62-63	21.415034558664072	27.801791372637997	29.000816734374546	21.782357334323386
64-65	21.3602322837251	27.661419628152384	29.101232664447647	21.87711542367487
66-67	21.336748663818554	27.602198507991595	29.044761684586984	22.016291143602874
68-69	21.5463609198188	27.76786581377941	28.79969123945672	21.886082026945072
70-71	21.527353439723658	27.59946985316256	28.820037980583436	22.053138726530346
72-73	21.425404879379133	27.7185065248511	28.860974964842605	21.99511363092716
74-75	21.45178187605977	27.723047121075783	28.742246251573096	22.08292475129135
76-77	21.45896518522911	27.73054555048785	28.616728129437647	22.193761134845396
78-79	21.48090959581835	27.715629376551654	28.687984341367358	22.115476686262635
80-81	21.42516239121339	27.67715069792878	28.704739120551437	22.192947790306388
82-83	21.33189733674819	27.639830800107113	28.809688651198574	22.218583211946118
84-85	21.324501945294553	27.568272949133004	28.658431428832525	22.448793676739914
86-87	21.381355564401204	27.659095997241838	28.59395762577489	22.365590812582063
88-89	21.306009525947843	27.48438989996	28.635897303679066	22.57370327041309
90-91	21.203581057393816	27.398290399963045	28.63769492258601	22.760433620057128
92-93	21.20445150222695	27.50019927898607	28.572001520894357	22.723347697892628
94-95	21.09181114782126	27.340513059094874	28.684065328848668	22.8836104642352
96-97	21.05072125657357	27.350211699942772	28.64577853791788	22.953288505565776
98-99	21.047813931156796	27.308324807178558	28.58524271216088	23.058618549503766
100	21.078623269522176	27.189133496653454	28.57807072841319	23.15417250541118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1095.0
1	807.5
2	1110.5
3	1703.0
4	1147.5
5	699.0
6	1516.0
7	3950.5
8	6633.0
9	7802.0
10	6568.5
11	4669.5
12	4424.5
13	4892.5
14	5499.5
15	6228.0
16	6942.0
17	7590.5
18	8530.0
19	9788.0
20	11280.5
21	13714.0
22	17479.5
23	22888.5
24	29897.0
25	38358.0
26	49526.0
27	64066.5
28	83185.0
29	103642.0
30	120728.0
31	137787.0
32	155329.5
33	171121.0
34	186351.5
35	201395.5
36	214681.0
37	226917.0
38	238465.5
39	246245.5
40	250468.5
41	251756.0
42	248775.0
43	246057.0
44	243600.5
45	239550.5
46	235029.5
47	227544.5
48	219221.0
49	214505.5
50	209838.0
51	202273.5
52	192580.5
53	184587.0
54	178475.0
55	168322.5
56	155239.0
57	140272.5
58	126401.0
59	114672.0
60	100100.0
61	84249.5
62	71117.0
63	58546.0
64	46318.0
65	36352.5
66	28278.5
67	21892.0
68	16864.5
69	12821.5
70	9346.0
71	6762.5
72	5125.0
73	3679.5
74	2495.5
75	1669.5
76	1020.5
77	605.5
78	387.0
79	235.0
80	144.5
81	69.0
82	33.5
83	20.0
84	14.0
85	9.0
86	7.0
87	5.0
88	2.5
89	3.5
90	3.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05792483007140911
2	2.578256531368358E-4
3	1.4323647396490878E-5
4	0.11960245576069883
5	0.001747484982371887
6	0.0013320992078736517
7	0.0
8	0.0
9	4.297094218947264E-5
10-11	0.0
12-13	0.0
14-15	2.8647294792981755E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.289128265684179E-4
30-31	3.0079659532630847E-4
32-33	7.161823698245439E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.1485471094736315E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.3607465026666335E-4
80-81	2.721493005333267E-4
82-83	2.2201653464560858E-4
84-85	0.0
86-87	1.4323647396490878E-5
88-89	6.445641328420895E-5
90-91	6.445641328420895E-5
92-93	6.230786617473532E-4
94-95	6.302404854455987E-4
96-97	7.161823698245439E-6
98-99	4.297094218947264E-5
100	0.0011602154391157611
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6981462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.013671877860375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56550992231969	41.20674334316297
2	15.25546596967233	17.090293303381817
3	4.839881990315922	8.132986851993653
4	2.170961106366672	4.86414012286478
5	1.1511291878874805	3.2239486309678607
6	0.6715414481358534	2.256930139695893
7	0.46454339933364625	1.8214547080310501
8	0.3183307150100179	1.426469777537266
9	0.23725991765177432	1.1960819259403188
>10	1.2289653016115927	12.137276446317209
>50	0.06782269956434231	2.5743805918993194
>100	0.027157522147364343	2.563194680261999
>500	9.198567479670158E-4	0.3282624871160988
>1k	4.087705904883109E-4	0.3675356327416036
>5k	2.5548161235992422E-5	0.08418845503828308
>10k+	7.664448370797727E-5	0.7261129030498867
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19613	0.2809296963873756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19604	0.2808007835608072	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11523	0.1650513889497644	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.297094218947264E-5	0.0	0.0	0.0
6	0.0	4.297094218947264E-5	0.0	0.0	0.0
7	0.0	4.297094218947264E-5	0.0	0.0	0.0
8	0.0	4.297094218947264E-5	0.0	0.0	0.0
9	0.0	4.297094218947264E-5	0.0	1.4323647396490878E-5	0.0
10-11	0.0	4.297094218947264E-5	0.0	1.4323647396490878E-5	0.0
12-13	0.0	5.729458958596352E-5	0.0	3.5809118491227195E-5	0.0
14-15	0.0	8.594188437894526E-5	0.0	6.445641328420895E-5	0.0
16-17	0.0	1.1458917917192702E-4	0.0	8.594188437894526E-5	0.0
18-19	0.0	1.289128265684179E-4	0.0	1.0026553177543615E-4	0.0
20-21	0.0	1.289128265684179E-4	0.0	1.0742735547368158E-4	0.0
22-23	0.0	1.4323647396490878E-4	0.0	1.7904559245613598E-4	0.0
24-25	0.0	1.6472194505964512E-4	0.0	4.0822395079999005E-4	0.0
26-27	0.0	1.7188376875789055E-4	0.0	8.30771548996471E-4	0.0
28-29	0.0	1.7904559245613598E-4	0.0	0.0025424474128771307	0.0
30-31	0.0	1.862074161543814E-4	0.0	0.007627342238631393	0.0
32-33	0.0	2.005310635508723E-4	0.0	0.019823927996743377	0.0
34-35	7.161823698245439E-6	2.005310635508723E-4	0.0	0.03946881040103062	0.0
36-37	1.4323647396490878E-5	2.220165346456086E-4	0.0	0.07101664379180178	0.0
38-39	1.4323647396490878E-5	2.5066382943859033E-4	0.0	0.11486849029615859	0.0
40-41	1.4323647396490878E-5	2.578256531368358E-4	0.0	0.1618858628751399	0.0
42-43	1.4323647396490878E-5	2.578256531368358E-4	0.0	0.21042154207814925	0.0
44-45	1.4323647396490878E-5	2.6498747683508124E-4	0.0	0.27304452849560734	0.0
46-47	1.4323647396490878E-5	2.7931112423157216E-4	0.0	0.35100097945100894	0.0
48-49	2.8647294792981755E-5	2.8647294792981756E-4	0.0	0.4335982921628736	0.0
50-51	2.8647294792981755E-5	3.294438901192902E-4	0.0	0.5192322181227944	0.0
52-53	3.5809118491227195E-5	3.294438901192902E-4	0.0	0.6066780854783711	0.0
54-55	4.297094218947264E-5	3.437675375157811E-4	0.0	0.7072945466150213	0.0
56-57	4.297094218947264E-5	3.437675375157811E-4	0.0	0.832963067048134	0.0
58-59	4.297094218947264E-5	3.509293612140265E-4	0.0	0.969990239866664	0.0
60-61	4.297094218947264E-5	3.724148323087628E-4	0.0	1.1148954187532638	0.0
62-63	5.729458958596351E-5	3.724148323087628E-4	0.0	1.264871168818222	0.0
64-65	5.729458958596351E-5	3.724148323087628E-4	0.0	1.4305098273112424	0.0
66-67	5.729458958596351E-5	4.010621271017446E-4	0.0	1.6292217876427602	0.0
68-69	5.729458958596351E-5	4.010621271017446E-4	0.0	1.8488462731731548	0.0
70-71	6.445641328420895E-5	4.010621271017446E-4	0.0	2.0918755985494153	0.0
72-73	7.161823698245439E-5	4.2970942189472636E-4	0.0	2.347853501172104	0.0
74-75	7.161823698245439E-5	4.440330692912172E-4	0.0	2.613600131319199	0.0
76-77	7.161823698245439E-5	4.440330692912172E-4	0.0	2.906260894924301	0.0
78-79	7.161823698245439E-5	4.72680364084199E-4	0.0	3.2382185851616754	0.0
80-81	7.161823698245439E-5	4.72680364084199E-4	0.0	3.6006784825298768	0.0
82-83	7.161823698245439E-5	4.72680364084199E-4	0.0	3.9779848404245417	0.0
84-85	7.161823698245439E-5	5.013276588771808E-4	0.0	4.36221668183541	0.0
86-87	7.161823698245439E-5	5.299749536701625E-4	0.0	4.774709079559553	0.0
88	7.161823698245439E-5	5.87269543256126E-4	0.0	5.114544775864998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12820	0.0	55.85339	1
GTATCAA	22240	0.0	38.398197	1
ATCAACG	27650	0.0	30.342813	3
TCAACGC	27905	0.0	29.998089	4
GTGGTAT	5385	0.0	29.985878	1
TATCAAC	28815	0.0	29.132374	2
CAACGCA	28945	0.0	28.91834	5
AACGCAG	30435	0.0	27.610292	6
TGGTATC	6075	0.0	24.785822	2
ACGCAGA	34845	0.0	23.940237	7
CGCAGAG	34980	0.0	23.659746	8
GTACATG	20225	0.0	22.368826	1
GCAGAGT	38110	0.0	21.346598	9
GAGTACT	24960	0.0	21.097832	12-13
TACATGG	20665	0.0	21.03954	2
CAGAGTA	36535	0.0	20.04144	10-11
ACATGGG	20810	0.0	19.852814	3
AGAGTAC	34980	0.0	19.776915	10-11
AGTACTT	26605	0.0	18.81295	12-13
GTACTTT	28290	0.0	18.390148	14-15
>>END_MODULE