FastQCFastQC Report
Wed 25 May 2016
SRR2049496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6826319
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74900.045.228781
GTATCAA124200.036.463671
TCAACGC131350.032.8760074
ATCAACG131750.032.8538673
CAACGCA140750.030.6071745
TATCAAC149200.029.697222
AACGCAG154000.028.239086
GTACATG223350.024.1486781
ACGCAGA180700.023.754477
CGCAGAG186250.023.0041338
TACATGG227650.022.9019932
ACATGGG233950.021.4760883
GTGGTAT42650.020.877041
GCAGAGT206600.019.4900029
CATGGGG157100.018.2706244
TACCTGG43750.017.7759272
GTACCTG49800.017.2630941
AGAGTAC180200.017.08547810-11
CAGAGTA198000.017.0576210-11
GAGTACA172900.016.9885221
Produced by FastQC (version 0.11.5)