##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049496_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6826319 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.752723246598936 31.0 31.0 34.0 27.0 34.0 2 30.981374149083862 31.0 31.0 34.0 27.0 34.0 3 31.035593853729953 31.0 31.0 34.0 27.0 34.0 4 34.614721052444224 37.0 35.0 37.0 32.0 37.0 5 34.642228849838396 37.0 35.0 37.0 32.0 37.0 6 34.59516043712578 37.0 35.0 37.0 31.0 37.0 7 30.69199681409556 37.0 33.0 37.0 2.0 37.0 8 32.42705329182536 37.0 33.0 37.0 17.0 37.0 9 35.17574361819305 39.0 34.0 39.0 28.0 39.0 10-11 35.73879341120741 39.0 36.0 39.0 30.0 39.0 12-13 35.90808281886622 39.0 37.0 39.0 30.0 39.0 14-15 37.09231161040086 40.0 37.0 41.0 31.0 41.0 16-17 37.10224771212714 40.0 37.0 41.0 31.0 41.0 18-19 37.089387193888825 40.0 37.0 41.0 31.0 41.0 20-21 37.16946461189406 40.0 37.0 41.0 31.0 41.0 22-23 37.125652785930455 40.0 37.0 41.0 31.0 41.0 24-25 37.104826627645146 40.0 37.0 41.0 30.5 41.0 26-27 37.14130133678195 40.0 37.0 41.0 31.0 41.0 28-29 37.12832699438746 40.0 37.0 41.0 31.0 41.0 30-31 37.05430261902498 40.0 37.0 41.0 30.0 41.0 32-33 37.01412584146742 40.0 37.0 41.0 30.0 41.0 34-35 36.96031741264949 40.0 37.0 41.0 30.0 41.0 36-37 36.78461429944894 40.0 37.0 41.0 30.0 41.0 38-39 36.676388475252914 40.0 36.5 41.0 30.0 41.0 40-41 36.600034513476444 40.0 36.0 41.0 30.0 41.0 42-43 36.474574291063746 40.0 36.0 41.0 30.0 41.0 44-45 36.342129557672294 40.0 36.0 41.0 30.0 41.0 46-47 36.18302404267952 40.0 35.5 41.0 29.0 41.0 48-49 36.09910194645167 39.5 35.0 41.0 29.0 41.0 50-51 35.95655162028027 39.0 35.0 41.0 29.0 41.0 52-53 35.836279479467635 39.0 35.0 41.0 28.0 41.0 54-55 35.71304637243 39.0 35.0 41.0 28.0 41.0 56-57 35.51287165161781 39.0 35.0 41.0 28.0 41.0 58-59 35.33067631325169 39.0 35.0 40.5 28.0 41.0 60-61 35.10652988821648 38.0 34.0 40.0 27.5 41.0 62-63 34.86775515471808 38.0 34.0 40.0 27.0 41.0 64-65 34.50449034098758 37.5 34.0 40.0 26.5 41.0 66-67 31.03724130091196 36.0 30.0 40.0 6.5 41.0 68-69 31.38831125530465 36.0 30.0 39.0 11.0 41.0 70-71 31.5435534876117 35.0 30.0 39.0 15.5 41.0 72-73 32.764495184007664 35.0 31.5 39.0 23.5 40.5 74-75 32.8487065283647 35.0 33.0 38.5 25.0 40.0 76-77 32.553708374894285 35.0 33.0 37.0 24.0 39.5 78-79 29.208660040645626 35.0 29.0 36.5 6.5 39.0 80-81 30.979596031184595 35.0 30.0 36.0 18.5 39.0 82-83 31.391389195260285 35.0 32.0 36.0 20.5 37.5 84-85 29.57901535219787 35.0 30.0 35.5 11.0 37.0 86-87 29.214853275974946 34.0 28.0 35.0 10.5 37.0 88-89 30.45651851312545 34.0 30.5 35.0 18.0 36.0 90-91 30.614996530340875 35.0 31.5 35.0 18.0 36.0 92-93 30.473514202896173 35.0 32.0 35.0 12.5 36.0 94-95 30.275359238265892 35.0 32.0 35.0 4.5 36.0 96-97 30.09343578874647 35.0 32.0 35.0 2.0 35.0 98-99 29.913065958388408 35.0 31.5 35.0 2.0 35.0 100 29.711947390680102 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3274909041419569 1101 2 0.3136109121471762 1101 3 0.34182251028210686 1101 4 0.34156393624449066 1101 5 0.39727478902693747 1101 6 0.37900628436622696 1101 7 -12.067973840923855 1101 8 -6.4992664618902225 1101 9 -2.1936491723947 1101 10-11 -0.1175148727779245 1101 12-13 0.37003130921210214 1101 14-15 0.5584633460424229 1101 16-17 0.6896076276745546 1101 18-19 0.6501222576145125 1101 20-21 0.6454411642350948 1101 22-23 0.5642364019749095 1101 24-25 0.61302741926729 1101 26-27 0.5569355141537358 1101 28-29 0.5292033591156269 1101 30-31 0.5519507430093213 1101 32-33 0.5815039952443399 1101 34-35 0.5929320940018741 1101 36-37 0.6168285727213672 1101 38-39 0.6235424995151604 1101 40-41 0.6115511616012625 1101 42-43 0.6003425116939525 1101 44-45 0.5318483036847965 1101 46-47 0.5805023986418902 1101 48-49 0.5203425815927076 1101 50-51 0.583920475782584 1101 52-53 0.5620509786680188 1101 54-55 0.47440971541234234 1101 56-57 0.45941443316842623 1101 58-59 0.4242904380357331 1101 60-61 0.3999651260249024 1101 62-63 0.4383289355557167 1101 64-65 0.33681729589944354 1101 66-67 -10.213828127774295 1101 68-69 -8.11736932689658 1101 70-71 -6.639526392693995 1101 72-73 -1.4757076250942838 1101 74-75 -0.23218459585682893 1101 76-77 0.07057514815362254 1101 78-79 3.231223988628443 1101 80-81 1.0892383457195365 1101 82-83 0.40703300995597047 1101 84-85 -5.351195243951501 1101 86-87 -5.872262332517106 1101 88-89 -1.2893696778568255 1101 90-91 0.003388776913890723 1101 92-93 0.3364083214383591 1101 94-95 0.38642849913226485 1101 96-97 0.49619041765659944 1101 98-99 0.524095021634281 1101 100 0.5670642499238134 1102 1 0.1825240063477409 1102 2 0.2321196546146851 1102 3 0.2659019232537325 1102 4 0.2988999640360035 1102 5 0.2766539276809823 1102 6 0.2862045209763764 1102 7 -12.168066732276106 1102 8 -6.60170482475392 1102 9 -2.350056040733506 1102 10-11 -0.27460762289597085 1102 12-13 0.29225561793742116 1102 14-15 0.4933744914588445 1102 16-17 0.47289024083055864 1102 18-19 0.49439977790726175 1102 20-21 0.4975361031319778 1102 22-23 0.44495937354257364 1102 24-25 0.4944646424201409 1102 26-27 0.4904580322260941 1102 28-29 0.4671870511323064 1102 30-31 0.45242199798413196 1102 32-33 0.44361725242831085 1102 34-35 0.43560052808695815 1102 36-37 0.6242129784977735 1102 38-39 0.5933374393957678 1102 40-41 0.5226247312711862 1102 42-43 0.5152293170465185 1102 44-45 0.4509507041041374 1102 46-47 0.46226574281489263 1102 48-49 0.39594309045744325 1102 50-51 0.3793221781817451 1102 52-53 0.4148302183642514 1102 54-55 0.3542952946271569 1102 56-57 0.40803750507560466 1102 58-59 0.39763924506343074 1102 60-61 0.31799773004635057 1102 62-63 0.25825839925923333 1102 64-65 0.17118699083355438 1102 66-67 -10.31285385769515 1102 68-69 -6.746365165757489 1102 70-71 -6.321095346605269 1102 72-73 -1.1603603681295418 1102 74-75 -0.20998078717085633 1102 76-77 -0.0453654856998611 1102 78-79 3.121777844533014 1102 80-81 1.0310730412719948 1102 82-83 0.3620827651500136 1102 84-85 -5.488643616174599 1102 86-87 -5.9806533481948065 1102 88-89 -1.3959338712619633 1102 90-91 -0.15123290162300762 1102 92-93 0.20077631945405372 1102 94-95 0.22548764394159093 1102 96-97 0.2991092403114095 1102 98-99 0.32887623860936444 1102 100 0.43585890382272297 1103 1 0.17309672036597945 1103 2 0.19083854430008174 1103 3 0.2549921919640177 1103 4 0.2788306819351476 1103 5 0.20444583753630496 1103 6 0.24957738421721132 1103 7 -12.156250582053858 1103 8 -6.612823775855148 1103 9 -2.3459281889739927 1103 10-11 -0.3461329477784716 1103 12-13 0.24791623167922694 1103 14-15 0.4302798259601275 1103 16-17 0.4043828993452081 1103 18-19 0.4320424143648296 1103 20-21 0.45761934818636973 1103 22-23 0.43765824397691233 1103 24-25 0.5169964810148855 1103 26-27 0.5047573436841262 1103 28-29 0.4644256468345134 1103 30-31 0.4891086777046212 1103 32-33 0.5095937888232598 1103 34-35 0.4857667719928003 1103 36-37 0.5333754772098516 1103 38-39 0.5832648034120567 1103 40-41 0.5925022986325601 1103 42-43 0.5954513132735428 1103 44-45 0.5184084632238921 1103 46-47 0.5381525803919374 1103 48-49 0.48840702256816115 1103 50-51 0.49686520615258445 1103 52-53 0.4430737493816892 1103 54-55 0.4727645663487081 1103 56-57 0.5294968673812903 1103 58-59 0.4645647958202801 1103 60-61 0.41897778642682937 1103 62-63 0.3530738488570222 1103 64-65 0.21504008937359487 1103 66-67 -10.294244829964093 1103 68-69 -8.226221205959057 1103 70-71 -6.746058188509082 1103 72-73 -1.5760330694244864 1103 74-75 -0.33526411554640134 1103 76-77 -0.0373143856869973 1103 78-79 3.062208739959658 1103 80-81 0.9817905827011231 1103 82-83 0.276017648811532 1103 84-85 -5.50028747702811 1103 86-87 -6.001857308664391 1103 88-89 -1.43918050005216 1103 90-91 -0.1477020604031729 1103 92-93 0.2118557684720841 1103 94-95 0.28169852865840994 1103 96-97 0.3852511211704268 1103 98-99 0.40874390178248277 1103 100 0.4762256167957801 1104 1 0.09277561744224272 1104 2 0.1038995982791775 1104 3 0.12957346081888232 1104 4 0.16614075609570023 1104 5 0.16129704571307002 1104 6 0.20841371024032895 1104 7 -12.180590419361426 1104 8 -6.629625748815279 1104 9 -2.4410434257041373 1104 10-11 -0.2984513711618888 1104 12-13 0.23163494693861963 1104 14-15 0.42510754028489117 1104 16-17 0.43481608783020675 1104 18-19 0.41523523527920503 1104 20-21 0.3884471831802472 1104 22-23 0.4228997711211875 1104 24-25 0.5088772710323184 1104 26-27 0.4538644048802709 1104 28-29 0.4277720413883017 1104 30-31 0.39745624590675277 1104 32-33 0.3894847204942309 1104 34-35 0.3870710426350499 1104 36-37 0.47887874937574537 1104 38-39 0.5189790664252243 1104 40-41 0.4611036715060166 1104 42-43 0.3916313792309154 1104 44-45 0.38546108438952587 1104 46-47 0.36517125681221785 1104 48-49 0.36668493311390904 1104 50-51 0.3489254723016231 1104 52-53 0.3378123592543645 1104 54-55 0.3335449161307267 1104 56-57 0.37806126566542986 1104 58-59 0.3549740835481501 1104 60-61 0.2724153300476786 1104 62-63 0.2254605289849536 1104 64-65 0.10613060770240423 1104 66-67 -10.312489777462186 1104 68-69 -8.34379516358755 1104 70-71 -6.821180218425837 1104 72-73 -1.7476932074988056 1104 74-75 -0.49794438836150334 1104 76-77 -0.14432044835129432 1104 78-79 2.973628134078645 1104 80-81 0.908941026561159 1104 82-83 0.28368466744658605 1104 84-85 -5.50666205483126 1104 86-87 -5.974817520194517 1104 88-89 -1.4956772628964465 1104 90-91 -0.17157250899714782 1104 92-93 0.18553759338857034 1104 94-95 0.30201774890188204 1104 96-97 0.3241420792251475 1104 98-99 0.3365830978840627 1104 100 0.36329976064919833 1105 1 0.030509673056673847 1105 2 0.07140190322573758 1105 3 0.01963840573626996 1105 4 0.09748135249432721 1105 5 0.05865589821738837 1105 6 0.08356532192244259 1105 7 -12.181905448464523 1105 8 -6.689449330411932 1105 9 -2.5891307555038594 1105 10-11 -0.5133730262025438 1105 12-13 0.06735998326426085 1105 14-15 0.17621524999244542 1105 16-17 0.2471269167439516 1105 18-19 0.20734863129573 1105 20-21 0.2581667944629089 1105 22-23 0.27419122338847046 1105 24-25 0.3294221102743933 1105 26-27 0.29140911039286266 1105 28-29 0.24145938883799545 1105 30-31 0.2880133723912337 1105 32-33 0.2568499769180832 1105 34-35 0.33469253518761377 1105 36-37 0.3696196426112479 1105 38-39 0.33128674563239713 1105 40-41 0.2565102913667161 1105 42-43 0.22159626508283736 1105 44-45 0.16117397765289354 1105 46-47 0.18221045328965602 1105 48-49 0.15216821615515386 1105 50-51 0.24248408045452408 1105 52-53 0.1975981492104566 1105 54-55 0.17254201859421414 1105 56-57 0.13517942833465924 1105 58-59 0.06358787785016062 1105 60-61 0.043502122026545464 1105 62-63 -0.012325247617539503 1105 64-65 -0.08248730589325959 1105 66-67 -10.323225293456694 1105 68-69 -8.379185934279626 1105 70-71 -6.765771857759567 1105 72-73 -1.8046362542240004 1105 74-75 -0.6629388398461558 1105 76-77 -0.35649351751825975 1105 78-79 2.7587194504102044 1105 80-81 0.687948861250625 1105 82-83 0.03275856662656196 1105 84-85 -5.5483492195882995 1105 86-87 -6.0808029012983695 1105 88-89 -1.642054418147275 1105 90-91 -0.3825403001267986 1105 92-93 -0.02252229767706382 1105 94-95 0.0223368882027124 1105 96-97 0.05612256439801655 1105 98-99 0.07158384684654706 1105 100 0.09553106116545962 1106 1 0.16809478927709165 1106 2 0.21156880307381698 1106 3 0.21135376028210828 1106 4 0.18531393624449066 1106 5 0.18611105833196007 1106 6 0.23675845618089397 1106 7 -12.167865250190264 1106 8 -6.619355747604509 1106 9 -2.505687661776939 1106 10-11 -0.3737301840713627 1106 12-13 0.21985484927001409 1106 14-15 0.32149272586867994 1106 16-17 0.35170041242359673 1106 18-19 0.4237497913982935 1106 20-21 0.40787781964048975 1106 22-23 0.3769324685772304 1106 24-25 0.46937092795454305 1106 26-27 0.40885545865180717 1106 28-29 0.3950108518762363 1106 30-31 0.38656253113673245 1106 32-33 0.43587175586209526 1106 34-35 0.45642449265052676 1106 36-37 0.4585243980109439 1106 38-39 0.47427096235300326 1106 40-41 0.4329827494390983 1106 42-43 0.4101383007866204 1106 44-45 0.30072348466934784 1106 46-47 0.38594550916312187 1106 48-49 0.33287727029927083 1106 50-51 0.43052701535787463 1106 52-53 0.3705135148649319 1106 54-55 0.37876541039303646 1106 56-57 0.3337387574927462 1106 58-59 0.315236685622601 1106 60-61 0.2837835985152495 1106 62-63 0.19661953642444985 1106 64-65 0.09211109416198582 1106 66-67 -10.291370960785876 1106 68-69 -8.273501686935191 1106 70-71 -6.793295396072374 1106 72-73 -1.7563377529900421 1106 74-75 -0.5182940311850146 1106 76-77 -0.2555952186417443 1106 78-79 2.9000373332230396 1106 80-81 0.7804183650245591 1106 82-83 0.050043989685697454 1106 84-85 -5.610695424936061 1106 86-87 -6.080236210412856 1106 88-89 -1.6264865938799957 1106 90-91 -0.37142873032549417 1106 92-93 -0.006453346032685658 1106 94-95 0.06834663378477757 1106 96-97 0.2181272540465642 1106 98-99 0.26612717655705964 1106 100 0.37160394586976153 1107 1 0.0684574995916023 1107 2 0.09173817310000487 1107 3 0.08355948599648144 1107 4 0.012628066365536483 1107 5 0.12605604605126075 1107 6 0.06664124309515529 1107 7 -12.140085544525672 1107 8 -6.6853780013783926 1107 9 -2.6046370060339115 1107 10-11 -0.5844818081487588 1107 12-13 0.08739079827747531 1107 14-15 0.16949050289962742 1107 16-17 0.21330942767238525 1107 18-19 0.27729435878961795 1107 20-21 0.24273993224927892 1107 22-23 0.29450803200975884 1107 24-25 0.3424844876643647 1107 26-27 0.2618501541046854 1107 28-29 0.2371354631212057 1107 30-31 0.24218305860657097 1107 32-33 0.2574846700643221 1107 34-35 0.2409381670848063 1107 36-37 0.3447494703431886 1107 38-39 0.3585634026526705 1107 40-41 0.33992319215003164 1107 42-43 0.32920342589795837 1107 44-45 0.25618473797580066 1107 46-47 0.3163045677437566 1107 48-49 0.2963643377061018 1107 50-51 0.35471035611691804 1107 52-53 0.2369514897591003 1107 54-55 0.25017328288539886 1107 56-57 0.20620016843587052 1107 58-59 0.20622552370529235 1107 60-61 0.2099280745356893 1107 62-63 0.14312400243203172 1107 64-65 0.03524199846740572 1107 66-67 -10.245525499098342 1107 68-69 -8.258864893513309 1107 70-71 -6.7785655577849795 1107 72-73 -1.8454022833148471 1107 74-75 -0.5708799294896565 1107 76-77 -0.32018594803612643 1107 78-79 2.8322095465477872 1107 80-81 0.7785014099958936 1107 82-83 0.10242360562145336 1107 84-85 -5.54756710412061 1107 86-87 -5.973971370397663 1107 88-89 -1.5535922385501415 1107 90-91 -0.2943864185617464 1107 92-93 0.07554063658860244 1107 94-95 0.19359598800350852 1107 96-97 0.1892542723524855 1107 98-99 0.2031317294107744 1107 100 0.27106939515800477 1108 1 0.11474601965732845 1108 2 0.1848518936014436 1108 3 0.16229128643545465 1108 4 0.13644363401958515 1108 5 0.09860445093232073 1108 6 0.16870086320540878 1108 7 -12.211548938436213 1108 8 -6.648502649436033 1108 9 -2.6032964624166155 1108 10-11 -0.510432545489877 1108 12-13 0.10853854295042709 1108 14-15 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-0.8739798860009138 2216 2 -0.6965688221042257 2216 3 -0.7812538136098119 2216 4 -0.7698861672449269 2216 5 -0.6543273617022791 2216 6 -0.8331476504474224 2216 7 3.132747335956804 2216 8 1.2738950588531779 2216 9 0.0106614753663834 2216 10-11 -0.6381149388213032 2216 12-13 -0.9860710832469479 2216 14-15 -1.1638739257628217 2216 16-17 -1.3679222191096017 2216 18-19 -1.788156102707127 2216 20-21 -1.4619710067608054 2216 22-23 -1.278544077244561 2216 24-25 -1.243789972074815 2216 26-27 -1.76561177962504 2216 28-29 -1.9329785086317344 2216 30-31 -1.7736218418933234 2216 32-33 -2.6783680743522993 2216 34-35 -1.7385704047464259 2216 36-37 -1.45761259267843 2216 38-39 -1.734893420519498 2216 40-41 -1.7461966537719391 2216 42-43 -1.3174313440982175 2216 44-45 -1.4079091546050222 2216 46-47 -1.6220229275923188 2216 48-49 -1.7126780407541773 2216 50-51 -2.2362307150344023 2216 52-53 -1.883482617286667 2216 54-55 -1.5301979740667164 2216 56-57 -1.571577891366715 2216 58-59 -1.9029552406973167 2216 60-61 -2.0738797942948324 2216 62-63 -1.8451945245152146 2216 64-65 -1.554805073594899 2216 66-67 1.827074127881687 2216 68-69 1.078026475942913 2216 70-71 0.7455391861609897 2216 72-73 -1.0022737914672923 2216 74-75 -1.8105546383365976 2216 76-77 -1.547306885293576 2216 78-79 0.9832510867744944 2216 80-81 -0.4733686322697963 2216 82-83 -1.313439998202206 2216 84-85 0.41425314691687376 2216 86-87 0.4403698272225185 2216 88-89 -0.8138349788085932 2216 90-91 -1.1567379306165222 2216 92-93 -1.2809372593151238 2216 94-95 -1.2483068628501073 2216 96-97 -1.1958786897502023 2216 98-99 -1.2245096324098306 2216 100 -2.487516178509182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109797.0 3 21066.0 4 12271.0 5 10819.0 6 9818.0 7 10814.0 8 11561.0 9 13417.0 10 14707.0 11 16820.0 12 18736.0 13 21101.0 14 24023.0 15 26237.0 16 28977.0 17 30208.0 18 27862.0 19 25909.0 20 24744.0 21 25329.0 22 27156.0 23 31575.0 24 37928.0 25 47678.0 26 59565.0 27 72327.0 28 89130.0 29 113959.0 30 139304.0 31 169636.0 32 226931.0 33 317846.0 34 483816.0 35 663490.0 36 742893.0 37 1247678.0 38 1408251.0 39 461925.0 40 1015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.221308911301634 20.29290906866462 14.584823263347833 22.900958756685913 2 15.39094739429688 20.3908572992312 40.53029809161071 23.687897214861202 3 18.43823796548592 25.501831179865814 30.27697694178313 25.782953912865135 4 12.932654394931722 17.190078092370413 38.31772154921364 31.559545963484233 5 13.298237955160735 37.74252288255769 35.07532785636434 13.883911305917234 6 30.475353201099676 37.730541380176206 17.95977672422406 13.834328694500057 7 27.15612157117983 32.75792694007498 22.216271544815918 17.869679943929267 8 24.973796257570886 36.26505662278989 20.532000061526606 18.229147058112613 9 24.88916793955864 17.697869085813306 21.67459504895684 35.73836792567121 10-11 23.83633111783964 27.401766603640997 28.589060956571178 20.172841321948184 12-13 24.21452908954299 24.892544576366856 28.711264445743012 22.181661888347147 14-15 21.741007122579532 27.158707350183896 25.947110001744715 25.15317552549185 16-17 21.326929655002687 30.38851293199742 26.642500276504716 21.64205713649518 18-19 21.300315733853047 29.40402433580968 28.41899858474238 20.876661345594897 20-21 22.407918528272706 28.556818103578223 27.994758229142235 21.040505139006836 22-23 22.16767433561529 28.35855386712247 28.04425549593661 21.429516301325634 24-25 21.893153542927017 28.478357076485878 28.0373434057213 21.59114597486581 26-27 21.63975994016083 28.53362832973304 28.055543429249646 21.771068300856484 28-29 21.64375851758466 28.553177781466115 28.189687590046702 21.613376110902525 30-31 21.949999699691737 28.777991476812026 27.794342748998396 21.477666074497836 32-33 21.62863640398821 28.62716035203751 28.17156335230794 21.57263989166634 34-35 21.835420855572945 28.385558561651386 28.095160280118943 21.683860302656726 36-37 22.048543892702316 28.49732146924908 27.80812845345448 21.646006184594125 38-39 21.863279499249487 28.606392015250986 27.98908957228525 21.541238913214283 40-41 21.76420569886975 28.42060475203435 28.081651918233817 21.73353763086208 42-43 21.719100527751873 28.468562975567778 28.02420164088555 21.788134855794798 44-45 21.840327580046644 28.53060537471172 27.935341556137068 21.69372548910457 46-47 21.812439127239625 28.602413060861664 27.84049137543268 21.744656436466034 48-49 21.753911609308798 28.45973788978298 27.91306882160272 21.8732816793055 50-51 21.749277590385343 28.42322835409853 27.876906426356406 21.95058762915972 52-53 21.756307609460677 28.501251446885895 27.842072830539127 21.900368113114304 54-55 21.807031609707707 28.64415057727559 27.772337209866148 21.776480603150556 56-57 21.723111887955664 28.49821580572649 27.753312773793816 22.025359532524032 58-59 21.683754395229915 28.25691251876476 27.991373062317503 22.06796002368782 60-61 21.871510927163595 28.50678394968813 27.717672548763243 21.904032574385027 62-63 21.69610531977458 28.570520298830477 27.788475617531976 21.94489876386297 64-65 21.74355262441812 28.429459733105155 27.827826024155446 21.999161618321285 66-67 21.63769168060724 28.388779729414765 27.791870812677725 22.181657777300266 68-69 22.05994584430272 28.515362587489474 27.59002654650037 21.834665021707437 70-71 21.848723307302585 28.359876939881218 27.71964261555418 22.071757137262015 72-73 21.707788606314317 28.479709507570973 27.887227773218548 21.92527411289616 74-75 21.740176615495457 28.535783722745943 27.737586692433563 21.986452969325036 76-77 21.816097234813213 28.480641582872686 27.582557998484585 22.120703183829516 78-79 21.931917853700597 28.521798972737656 27.70266503467568 21.843618138886068 80-81 21.771122595106156 28.48192735783062 27.741903112032436 22.005046935030784 82-83 21.72885667983502 28.469474339294592 27.866541009164713 21.935127971705672 84-85 21.85910761360538 28.40133559503505 27.66241093266742 22.077145858692155 86-87 21.895981984815602 28.44931075863466 27.689028723108688 21.96567853344105 88-89 21.763004645646404 28.38929878163118 27.743285552555342 22.104411020167074 90-91 21.775462759833385 28.3317147805827 27.674900751634507 22.217921707949408 92-93 21.858227981989767 28.45587578743859 27.59546080260521 22.090435427966433 94-95 21.840210192034117 28.27814332323193 27.711496797844287 22.17014968688967 96-97 21.8727242548814 28.31385479854341 27.68089092669989 22.1325300198753 98-99 21.81542266294094 28.311297438180226 27.657300266683933 22.215979632194895 100 21.895008502972818 28.26016635588721 27.637020337985845 22.207804803154126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3436.0 1 2656.5 2 2221.5 3 2593.5 4 2420.0 5 2054.0 6 1859.5 7 1824.0 8 1878.5 9 1871.0 10 1660.5 11 1522.5 12 1549.5 13 1565.5 14 1646.0 15 1841.5 16 2177.0 17 2725.0 18 3499.5 19 4679.5 20 6475.0 21 9161.5 22 13573.0 23 19987.5 24 27865.5 25 37538.5 26 50533.5 27 66741.5 28 86581.0 29 106848.5 30 123917.0 31 141227.5 32 158085.5 33 173620.5 34 189296.0 35 203532.0 36 214990.0 37 224639.5 38 233238.5 39 238746.0 40 242685.0 41 243395.0 42 239649.5 43 237470.0 44 234751.0 45 229687.0 46 225112.0 47 219670.5 48 214324.5 49 211097.5 50 206784.0 51 199444.0 52 189309.5 53 180452.5 54 173897.5 55 165029.5 56 151930.5 57 136693.0 58 122937.5 59 110804.5 60 97049.5 61 82070.5 62 69717.0 63 58293.5 64 47496.0 65 38404.5 66 30632.0 67 24079.0 68 18535.0 69 14137.0 70 10686.0 71 8042.5 72 6158.0 73 4489.5 74 3114.0 75 2181.5 76 1471.5 77 1041.0 78 744.5 79 574.5 80 415.0 81 253.5 82 173.5 83 134.0 84 121.0 85 99.5 86 67.5 87 59.5 88 56.5 89 39.5 90 30.5 91 30.5 92 29.0 93 24.5 94 19.5 95 18.5 96 19.0 97 16.5 98 18.5 99 23.0 100 40.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019542010855337993 2 0.0028712399757468117 3 0.001743252842417707 4 0.0025196595705533247 5 0.0023292201844068525 6 0.00470238791946289 7 11.567844983511613 8 5.859673419891453E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.980722406907734E-4 18-19 0.0 20-21 0.0 22-23 4.3947550649185894E-5 24-25 0.0 26-27 3.076328545443012E-4 28-29 0.0 30-31 0.0 32-33 1.4649183549728632E-5 34-35 6.592132597377883E-5 36-37 2.9298367099457264E-5 38-39 8.057050952350747E-5 40-41 7.324591774864316E-6 42-43 7.324591774864316E-5 44-45 2.6368530389511534E-4 46-47 0.0013550494783498984 48-49 0.0010034690731564114 50-51 4.7609846536618054E-4 52-53 3.0030826276943696E-4 54-55 4.3947550649185897E-4 56-57 0.001882420086140129 58-59 3.882033640678087E-4 60-61 0.001413646212548813 62-63 0.0025562825294276463 64-65 0.0010620658073553259 66-67 5.784259422977449 68-69 5.435469687250186 70-71 0.003288741706914078 72-73 0.0017139544753182498 74-75 0.0036256729285578364 76-77 0.0066067817809276124 78-79 5.4021354700827775 80-81 0.005771778318593081 82-83 0.0052297585272531215 84-85 5.784647626341517 86-87 0.004387430473143725 88-89 0.002094833247611194 90-91 0.003838086090028901 92-93 0.00454857149219074 94-95 0.0054275225051744575 96-97 0.0037794893558299866 98-99 0.006570158822053291 100 0.006899765451922185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6826319.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.31196110838475 #Duplication Level Percentage of deduplicated Percentage of total 1 90.7969561113866 78.36863344645718 2 6.5259665328475265 11.265379391555125 3 1.4118407310675782 3.6557622681341497 4 0.5325231701085423 1.8385247659088906 5 0.26862271422620965 1.159267663156068 6 0.15271763283080564 0.7908815031274247 7 0.0929406265182503 0.5615321419002492 8 0.050628627781952325 0.34958849216694055 9 0.03840313844707781 0.29831851728756853 >10 0.12791450397511087 1.6120664257639274 >50 0.0012792996594644525 0.0712332916454 >100 1.8904135418647687E-4 0.020174780577639346 >500 1.786979672456498E-5 0.008637312319470205 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.4649183549728632E-5 0.0 4 0.0 1.4649183549728632E-5 0.0 1.4649183549728632E-5 0.0 5 0.0 2.9298367099457264E-5 0.0 1.4649183549728632E-5 0.0 6 0.0 2.9298367099457264E-5 0.0 1.4649183549728632E-5 0.0 7 0.0 2.9298367099457264E-5 0.0 1.4649183549728632E-5 0.0 8 0.0 2.9298367099457264E-5 0.0 1.4649183549728632E-5 0.0 9 0.0 2.9298367099457264E-5 0.0 7.324591774864316E-5 0.0 10-11 0.0 2.9298367099457264E-5 0.0 7.324591774864316E-5 0.0 12-13 0.0 3.662295887432158E-5 0.0 8.789510129837179E-5 0.0 14-15 0.0 5.859673419891453E-5 0.0 1.0254428484810042E-4 0.0 16-17 0.0 7.324591774864316E-5 0.0 1.2451806017269336E-4 0.0 18-19 0.0 7.324591774864316E-5 0.0 1.39167243722422E-4 0.0 20-21 0.0 8.789510129837179E-5 0.0 2.0508856969620083E-4 0.0 22-23 0.0 1.0254428484810042E-4 0.0 3.5890499696835147E-4 0.0 24-25 0.0 1.1719346839782906E-4 0.0 8.203542787848033E-4 0.0 26-27 0.0 1.4649183549728632E-4 0.0 0.0014209708043236772 0.0 28-29 0.0 1.4649183549728632E-4 0.0 0.003662295887432158 0.0 30-31 0.0 1.4649183549728632E-4 0.0 0.010056664506888705 0.0 32-33 0.0 1.6114101904701495E-4 0.0 0.025072077645360555 0.0 34-35 2.9298367099457264E-5 1.6114101904701495E-4 0.0 0.04670159715653488 0.0 36-37 2.9298367099457264E-5 1.904393861464722E-4 0.0 0.0812223981914704 0.0 38-39 2.9298367099457264E-5 2.4903612034538676E-4 0.0 0.12815105769302607 0.0 40-41 2.9298367099457264E-5 2.78334487444844E-4 0.0 0.17781178992660612 0.0 42-43 2.9298367099457264E-5 2.78334487444844E-4 0.0 0.22903266020823226 0.0 44-45 2.9298367099457264E-5 2.9298367099457265E-4 0.0 0.29292507426037373 0.0 46-47 2.9298367099457264E-5 2.9298367099457265E-4 0.0 0.3702288158522917 0.0 48-49 2.9298367099457264E-5 3.0763285454430127E-4 0.0 0.4523814371991699 0.0 50-51 2.9298367099457264E-5 3.0763285454430127E-4 0.0 0.5357206424135761 0.0 52-53 2.9298367099457264E-5 3.0763285454430127E-4 0.0 0.6173019456020148 0.0 54-55 2.9298367099457264E-5 3.0763285454430127E-4 0.0 0.707116089945401 0.0 56-57 2.9298367099457264E-5 3.0763285454430127E-4 0.0 0.804555134326421 0.0 58-59 2.9298367099457264E-5 3.0763285454430127E-4 0.0 0.911384305362817 0.0 60-61 2.9298367099457264E-5 3.222820380940299E-4 0.0 1.0217439296346977 0.0 62-63 2.9298367099457264E-5 3.222820380940299E-4 0.0 1.1325796523719447 0.0 64-65 2.9298367099457264E-5 3.222820380940299E-4 0.0 1.250937730861977 0.0 66-67 2.9298367099457264E-5 3.222820380940299E-4 0.0 1.383468894436372 0.0 68-69 2.9298367099457264E-5 3.222820380940299E-4 0.0 1.5151064578142335 0.0 70-71 2.9298367099457264E-5 3.222820380940299E-4 0.0 1.6741013714712132 0.0 72-73 2.9298367099457264E-5 3.296066298688942E-4 0.0 1.8463684454242468 0.0 74-75 2.9298367099457264E-5 3.369312216437585E-4 0.0 2.002257732168684 0.0 76-77 2.9298367099457264E-5 3.369312216437585E-4 0.0 2.1670097749607073 0.0 78-79 3.662295887432158E-5 3.369312216437585E-4 0.0 2.3559402952015573 0.0 80-81 4.3947550649185894E-5 3.4425581341862284E-4 0.0 2.5836618534820888 0.0 82-83 4.3947550649185894E-5 3.5158040519348715E-4 0.0 2.8183490985405166 0.0 84-85 4.3947550649185894E-5 3.5158040519348715E-4 0.0 3.0570502198915697 0.0 86-87 4.3947550649185894E-5 3.882033640678087E-4 0.0 3.3353993565199636 0.0 88 4.3947550649185894E-5 3.9552795584267303E-4 0.0 3.5671933878273197 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7490 0.0 45.22878 1 GTATCAA 12420 0.0 36.46367 1 TCAACGC 13135 0.0 32.876007 4 ATCAACG 13175 0.0 32.853867 3 CAACGCA 14075 0.0 30.607174 5 TATCAAC 14920 0.0 29.69722 2 AACGCAG 15400 0.0 28.23908 6 GTACATG 22335 0.0 24.148678 1 ACGCAGA 18070 0.0 23.75447 7 CGCAGAG 18625 0.0 23.004133 8 TACATGG 22765 0.0 22.901993 2 ACATGGG 23395 0.0 21.476088 3 GTGGTAT 4265 0.0 20.87704 1 GCAGAGT 20660 0.0 19.490002 9 CATGGGG 15710 0.0 18.270624 4 TACCTGG 4375 0.0 17.775927 2 GTACCTG 4980 0.0 17.263094 1 AGAGTAC 18020 0.0 17.085478 10-11 CAGAGTA 19800 0.0 17.05762 10-11 GAGTACA 17290 0.0 16.988522 1 >>END_MODULE