FastQCFastQC Report
Wed 25 May 2016
SRR2049496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6826319
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252570.36999442891549605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235280.3446659905580152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142470.20870691803298383No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69460.10175322893641507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139750.058.3840641
GTATCAA258000.040.7489621
ATCAACG322950.032.2150543
TCAACGC326150.031.8268434
TATCAAC333100.031.2757992
CAACGCA337100.030.8126955
GTGGTAT54350.030.4057081
AACGCAG353400.029.4839576
TGGTATC60650.026.1459562
ACGCAGA401750.025.7825817
CGCAGAG402950.025.6241558
GCAGAGT434950.023.4364019
GTACATG220000.023.1553941
TACATGG226550.021.9333672
GAGTACT303750.021.34401512-13
CAGAGTA422150.020.86840810-11
ACATGGG230150.020.7520263
AGAGTAC405700.020.71832310-11
TATAACG7000.020.1664122
AGTACTT314850.019.68846112-13
Produced by FastQC (version 0.11.5)