##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049496_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6826319
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814475854409967	31.0	31.0	34.0	30.0	34.0
2	32.037819211202994	33.0	31.0	34.0	30.0	34.0
3	32.05196387687127	34.0	31.0	34.0	30.0	34.0
4	35.67710049881935	37.0	35.0	37.0	33.0	37.0
5	35.78204827521245	37.0	35.0	37.0	35.0	37.0
6	35.83345240678029	37.0	35.0	37.0	35.0	37.0
7	35.824167168279125	37.0	35.0	37.0	35.0	37.0
8	35.80999393670293	37.0	35.0	37.0	35.0	37.0
9	37.56657709667538	39.0	37.0	39.0	35.0	39.0
10-11	37.51984634764358	39.0	37.0	39.0	35.0	39.0
12-13	37.49850431835958	39.0	37.0	39.0	35.0	39.0
14-15	38.7761879425793	40.0	38.0	41.0	35.0	41.0
16-17	38.78310880285554	40.0	38.0	41.0	35.0	41.0
18-19	38.862512065433805	40.0	38.0	41.0	35.0	41.0
20-21	38.891130417432876	40.0	38.0	41.0	35.0	41.0
22-23	38.86239670311335	40.0	38.0	41.0	35.0	41.0
24-25	38.82065956776998	40.0	38.0	41.0	35.0	41.0
26-27	38.72888433722479	40.0	38.0	41.0	34.5	41.0
28-29	38.64022191169209	40.0	38.0	41.0	34.0	41.0
30-31	38.51905023776357	40.0	38.0	41.0	34.0	41.0
32-33	38.36656981603116	40.0	38.0	41.0	34.0	41.0
34-35	38.26368706179714	40.0	38.0	41.0	34.0	41.0
36-37	38.12898562167985	40.0	38.0	41.0	34.0	41.0
38-39	38.03921623059222	40.0	38.0	41.0	33.0	41.0
40-41	37.91750693162743	40.0	37.0	41.0	33.0	41.0
42-43	37.77106702748583	40.0	37.0	41.0	33.0	41.0
44-45	37.571182946475254	40.0	37.0	41.0	33.0	41.0
46-47	37.301897625938665	40.0	37.0	41.0	32.0	41.0
48-49	37.05217863390211	40.0	37.0	41.0	31.5	41.0
50-51	36.832601362461965	40.0	36.0	41.0	31.0	41.0
52-53	36.72947938706058	40.0	36.0	41.0	31.0	41.0
54-55	36.594745059526225	39.5	36.0	41.0	31.0	41.0
56-57	36.42062237349295	39.0	35.0	41.0	31.0	41.0
58-59	36.24342182074996	39.0	35.0	41.0	30.0	41.0
60-61	36.052454258290595	39.0	35.0	41.0	30.0	41.0
62-63	35.849929954928854	39.0	35.0	41.0	30.0	41.0
64-65	35.574201483991594	38.0	35.0	40.0	29.0	41.0
66-67	35.317141200110925	37.5	35.0	40.0	29.0	41.0
68-69	35.0127300672588	37.0	34.5	40.0	29.0	41.0
70-71	34.7232607353978	36.5	34.0	39.5	29.0	41.0
72-73	34.40354779786881	36.0	34.0	39.0	28.0	41.0
74-75	34.06834356847372	36.0	34.0	39.0	28.0	40.5
76-77	33.77820762551531	35.0	34.0	37.5	28.0	40.0
78-79	33.45716227149654	35.0	34.0	37.0	28.0	39.0
80-81	33.153730876040214	35.0	34.0	37.0	27.5	39.0
82-83	32.863344285551264	35.0	34.0	36.0	27.0	39.0
84-85	32.594113679715235	35.0	33.0	36.0	27.0	37.0
86-87	32.325286219410486	35.0	33.0	36.0	26.0	37.0
88-89	32.125127026732855	35.0	33.0	35.0	26.0	36.5
90-91	31.982161469453743	35.0	33.0	35.0	26.0	36.0
92-93	31.82900359622807	35.0	33.0	35.0	26.0	36.0
94-95	31.655357667873417	35.0	33.0	35.0	25.5	36.0
96-97	31.495370198785025	35.0	33.0	35.0	25.0	36.0
98-99	31.322272735862477	35.0	33.0	35.0	25.0	35.0
100	31.217249589420007	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3560686170809433
1101	2	0.39200943144509637
1101	3	0.4380852213053359
1101	4	0.3277163230903213
1101	5	0.27844899548777846
1101	6	0.2652122608556269
1101	7	0.26930214668578856
1101	8	0.30717065791081666
1101	9	0.2761217575179913
1101	10-11	0.31617899396419347
1101	12-13	0.2983502635345161
1101	14-15	0.41774230744248086
1101	16-17	0.46757271726316674
1101	18-19	0.4279436137333903
1101	20-21	0.4085572312831971
1101	22-23	0.41810365429257246
1101	24-25	0.4563348594109584
1101	26-27	0.43922046711383445
1101	28-29	0.408989808713514
1101	30-31	0.4188025904846455
1101	32-33	0.4603404504841109
1101	34-35	0.5154431956657675
1101	36-37	0.4762240927392227
1101	38-39	0.40829074531825427
1101	40-41	0.4391586545007584
1101	42-43	0.318080795859494
1101	44-45	0.3729740116899194
1101	46-47	0.4757204498478984
1101	48-49	0.5356327245807151
1101	50-51	0.5635078960345865
1101	52-53	0.5919715692661285
1101	54-55	0.5096525309515272
1101	56-57	0.48550184992286205
1101	58-59	0.48605748073098454
1101	60-61	0.5230765413171881
1101	62-63	0.5020671802812302
1101	64-65	0.46318536826310464
1101	66-67	0.48597190180888106
1101	68-69	0.49903822310944435
1101	70-71	0.4688334170324211
1101	72-73	0.47183728033261474
1101	74-75	0.49860944564142073
1101	76-77	0.4624087632787166
1101	78-79	0.46870854353878855
1101	80-81	0.44431793782013784
1101	82-83	0.4496630293058459
1101	84-85	0.4425622878201523
1101	86-87	0.5451755614014289
1101	88-89	0.5568986287123323
1101	90-91	0.5563688485902105
1101	92-93	0.5432883965280624
1101	94-95	0.5239602709587778
1101	96-97	0.5737472691250431
1101	98-99	0.5957652059104177
1101	100	0.642965494148747
1102	1	0.27828036579929716
1102	2	0.3417237308110188
1102	3	0.37451524924301083
1102	4	0.32895089272651745
1102	5	0.269807710692163
1102	6	0.23716479913642274
1102	7	0.26182042281340756
1102	8	0.2770596660953757
1102	9	0.3607544221085348
1102	10-11	0.26793284523122907
1102	12-13	0.26788609837622346
1102	14-15	0.3785642202454653
1102	16-17	0.4010115312331308
1102	18-19	0.391893056104216
1102	20-21	0.340266628908779
1102	22-23	0.35110866494486004
1102	24-25	0.3617790866560924
1102	26-27	0.3494949392773421
1102	28-29	0.36910203720657364
1102	30-31	0.43298275052197255
1102	32-33	0.4521613388121253
1102	34-35	0.45231238468632284
1102	36-37	0.49654662317835374
1102	38-39	0.42692549188366513
1102	40-41	0.4555210059589143
1102	42-43	0.46988428989534015
1102	44-45	0.49524998332503856
1102	46-47	0.49435449375557283
1102	48-49	0.525546775486859
1102	50-51	0.5122767694212769
1102	52-53	0.5191316972341511
1102	54-55	0.47962058813100583
1102	56-57	0.4820084991729914
1102	58-59	0.5017220930606641
1102	60-61	0.47698078874265804
1102	62-63	0.47148242148957564
1102	64-65	0.5480391065427739
1102	66-67	0.539645554532953
1102	68-69	0.5073713046570845
1102	70-71	0.5199523819051635
1102	72-73	0.5060195588830041
1102	74-75	0.47978085337402376
1102	76-77	0.4721893197555289
1102	78-79	0.44034962877960027
1102	80-81	0.4062203625514371
1102	82-83	0.3818553284581654
1102	84-85	0.3875707126863617
1102	86-87	0.4040333174780031
1102	88-89	0.3997036418941917
1102	90-91	0.3640344755576592
1102	92-93	0.41351998549014723
1102	94-95	0.40570007609773384
1102	96-97	0.45236481462916345
1102	98-99	0.4899665581049568
1102	100	0.5251319003636148
1103	1	0.28721771212270397
1103	2	0.2844474808124886
1103	3	0.37866570825131163
1103	4	0.31567231172565613
1103	5	0.26631865862917437
1103	6	0.24240107007568668
1103	7	0.2634246837770746
1103	8	0.2855960894612437
1103	9	0.35619995206629085
1103	10-11	0.3393019376883615
1103	12-13	0.29941044181956045
1103	14-15	0.37238213368556927
1103	16-17	0.3908645231976351
1103	18-19	0.3780532988974201
1103	20-21	0.3941721170339605
1103	22-23	0.37539049430699123
1103	24-25	0.39487854404838885
1103	26-27	0.4225341172073698
1103	28-29	0.4346962739580249
1103	30-31	0.3553899562500078
1103	32-33	0.3989416355187174
1103	34-35	0.3785840650906067
1103	36-37	0.4543353853036436
1103	38-39	0.48615039714189834
1103	40-41	0.4409765455000354
1103	42-43	0.43389877742136207
1103	44-45	0.4675370955532827
1103	46-47	0.45112321049182214
1103	48-49	0.4177280210781831
1103	50-51	0.45296566151136375
1103	52-53	0.3768766803431163
1103	54-55	0.3791375644990538
1103	56-57	0.43674549877182045
1103	58-59	0.4305675270582867
1103	60-61	0.3890576885483412
1103	62-63	0.44615711740785713
1103	64-65	0.4769860360209748
1103	66-67	0.4628812605966388
1103	68-69	0.4550924317961318
1103	70-71	0.4389605429849581
1103	72-73	0.4470128320197162
1103	74-75	0.4931802803956771
1103	76-77	0.4669333030444207
1103	78-79	0.4095993122148869
1103	80-81	0.3143309285872036
1103	82-83	0.35151752304214057
1103	84-85	0.3951929705799131
1103	86-87	0.45751142847574044
1103	88-89	0.48285948988038996
1103	90-91	0.45354750825188717
1103	92-93	0.47251344311044363
1103	94-95	0.5240531286433487
1103	96-97	0.5341575983363285
1103	98-99	0.5586024552626121
1103	100	0.5119101694792931
1104	1	0.23337022143230968
1104	2	0.2374185616429898
1104	3	0.3087025047628842
1104	4	0.2183615025530088
1104	5	0.1745995107367193
1104	6	0.19189315754687186
1104	7	0.13446235690533825
1104	8	0.15636898671498045
1104	9	0.19945445043365595
1104	10-11	0.2090919719231792
1104	12-13	0.18268494139461922
1104	14-15	0.263105529362754
1104	16-17	0.2913241655600203
1104	18-19	0.2848137958866701
1104	20-21	0.26238615820663824
1104	22-23	0.255233965485111
1104	24-25	0.2603056836915627
1104	26-27	0.2928635958386252
1104	28-29	0.27314565974872096
1104	30-31	0.2901597665538276
1104	32-33	0.30055176098886704
1104	34-35	0.2839462179607182
1104	36-37	0.3134596524418427
1104	38-39	0.3635081401412137
1104	40-41	0.36628556461302253
1104	42-43	0.32308805100694116
1104	44-45	0.41733562808170177
1104	46-47	0.41943483624929456
1104	48-49	0.48887766626206997
1104	50-51	0.5064823109983294
1104	52-53	0.4510353903903237
1104	54-55	0.4161952803024249
1104	56-57	0.4278517262816237
1104	58-59	0.4410361272860115
1104	60-61	0.42962200228338787
1104	62-63	0.42189735959257035
1104	64-65	0.4384428088093628
1104	66-67	0.4540116320178811
1104	68-69	0.42378727782774206
1104	70-71	0.4343240291680175
1104	72-73	0.38761230728061236
1104	74-75	0.40065306817447777
1104	76-77	0.3891851813484095
1104	78-79	0.3214242998314134
1104	80-81	0.3672125367028656
1104	82-83	0.3333698473577229
1104	84-85	0.3580890705915962
1104	86-87	0.405280611269923
1104	88-89	0.32362209040665846
1104	90-91	0.35638942218303526
1104	92-93	0.36939501934363506
1104	94-95	0.3432540812028755
1104	96-97	0.3163942880718835
1104	98-99	0.35130025385345576
1104	100	0.4068534628167697
1105	1	0.25445505391630263
1105	2	0.2360897822079906
1105	3	0.31603654086945454
1105	4	0.26530761286376503
1105	5	0.21884531132149476
1105	6	0.22681883608365894
1105	7	0.2145116994052998
1105	8	0.21491741613871795
1105	9	0.2627103703337639
1105	10-11	0.19955786819709687
1105	12-13	0.20542523739543128
1105	14-15	0.2881732214435786
1105	16-17	0.29672998130209294
1105	18-19	0.32370382767984296
1105	20-21	0.2742712880693787
1105	22-23	0.2517511109200754
1105	24-25	0.2722459116470972
1105	26-27	0.2775722204028952
1105	28-29	0.35948119726381833
1105	30-31	0.30759079426970004
1105	32-33	0.32143895543345735
1105	34-35	0.24305076654341207
1105	36-37	0.29018743825328386
1105	38-39	0.28736537240938276
1105	40-41	0.31377912876098435
1105	42-43	0.28782291538473714
1105	44-45	0.35057567171892856
1105	46-47	0.3439210857238564
1105	48-49	0.30159726999704617
1105	50-51	0.3642837277566642
1105	52-53	0.2886641703285022
1105	54-55	0.2898047550068412
1105	56-57	0.30085096106258646
1105	58-59	0.2843734963724103
1105	60-61	0.27962576161058195
1105	62-63	0.22383844488759763
1105	64-65	0.3157874387677069
1105	66-67	0.2860347374137362
1105	68-69	0.2873425746762166
1105	70-71	0.28397466332829424
1105	72-73	0.285207078129055
1105	74-75	0.31000953522361385
1105	76-77	0.21246861062119393
1105	78-79	0.23173703908332044
1105	80-81	0.24021649846281434
1105	82-83	0.23184743596171842
1105	84-85	0.22311300158796143
1105	86-87	0.301402722539045
1105	88-89	0.26839166638939105
1105	90-91	0.2171942559133555
1105	92-93	0.20156335475805776
1105	94-95	0.24177939439305618
1105	96-97	0.24663047929380966
1105	98-99	0.311449609939249
1105	100	0.36673774521007374
1106	1	0.16684021457129106
1106	2	0.2234886785493444
1106	3	0.2826044056682733
1106	4	0.22866950841850553
1106	5	0.13383026961904676
1106	6	0.18800726568188253
1106	7	0.19901679436918585
1106	8	0.17701863088378644
1106	9	0.15560414168787418
1106	10-11	0.1825867521688309
1106	12-13	0.19280157868895031
1106	14-15	0.25520611053128306
1106	16-17	0.2523549325141303
1106	18-19	0.2616550036947771
1106	20-21	0.22191389151485907
1106	22-23	0.20417538450492145
1106	24-25	0.26646697813682607
1106	26-27	0.2548394342952989
1106	28-29	0.2372354650841757
1106	30-31	0.2631830200213301
1106	32-33	0.26626769469260836
1106	34-35	0.2351916271329486
1106	36-37	0.2495605430287995
1106	38-39	0.2869602071908446
1106	40-41	0.2753088113540372
1106	42-43	0.2846665222108484
1106	44-45	0.3163831574428144
1106	46-47	0.2968247572803264
1106	48-49	0.3390352342332221
1106	50-51	0.3531616122507941
1106	52-53	0.33573212669855934
1106	54-55	0.29694138230288303
1106	56-57	0.3385680299421736
1106	58-59	0.299319124070756
1106	60-61	0.3389307884214432
1106	62-63	0.34473428935459083
1106	64-65	0.3266068812939906
1106	66-67	0.31551913877800075
1106	68-69	0.31743982783917346
1106	70-71	0.34682117041080573
1106	72-73	0.33402809838280945
1106	74-75	0.34910715317037955
1106	76-77	0.3386289008269756
1106	78-79	0.3155413138090566
1106	80-81	0.233027517935966
1106	82-83	0.26190663451820484
1106	84-85	0.29882736986647984
1106	86-87	0.3952675618840473
1106	88-89	0.4096299873030631
1106	90-91	0.3308213703083567
1106	92-93	0.3123189225898315
1106	94-95	0.33876028302440986
1106	96-97	0.28139839122929544
1106	98-99	0.3615018130532732
1106	100	0.36551616982442425
1107	1	0.12562469166452672
1107	2	0.17441161939289174
1107	3	0.22300871090104124
1107	4	0.18695538654419153
1107	5	0.16440094722686638
1107	6	0.15218460475290385
1107	7	0.15248034034052438
1107	8	0.1438741878867802
1107	9	0.14696593464450558
1107	10-11	0.1370920184014892
1107	12-13	0.12526404236775335
1107	14-15	0.14423202990523265
1107	16-17	0.1405661904730522
1107	18-19	0.1469437752927334
1107	20-21	0.20574393545973635
1107	22-23	0.19432222106159713
1107	24-25	0.22338233399249674
1107	26-27	0.20483319451021487
1107	28-29	0.241871310679052
1107	30-31	0.208974024987981
1107	32-33	0.23143714590897702
1107	34-35	0.17034673140486234
1107	36-37	0.25155933137930475
1107	38-39	0.2138368636074759
1107	40-41	0.23510380522189678
1107	42-43	0.2630902074498138
1107	44-45	0.2692372752813412
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2216	94-95	-1.26546213810769
2216	96-97	-1.3433095010020857
2216	98-99	-1.3222939401162606
2216	100	-1.3562620034915973
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	420.0
3	60.0
4	81.0
5	125.0
6	430.0
7	1102.0
8	2586.0
9	4204.0
10	5681.0
11	7989.0
12	11447.0
13	16071.0
14	19065.0
15	24752.0
16	32872.0
17	47509.0
18	49194.0
19	26465.0
20	18950.0
21	18423.0
22	18723.0
23	20575.0
24	23420.0
25	28723.0
26	36667.0
27	47199.0
28	62309.0
29	80890.0
30	105581.0
31	136701.0
32	176028.0
33	229293.0
34	311892.0
35	456498.0
36	751759.0
37	1376172.0
38	1913875.0
39	760180.0
40	2408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77442497671969	20.346572829148695	15.12822162766778	22.750780566463835
2	15.498873766974066	20.78563543098629	39.850630561581276	23.86486024045837
3	18.098614802298986	25.577199890189707	30.943885122257708	25.380300185253603
4	12.756280039835556	17.561216363084352	37.770749028678374	31.911754568401722
5	12.772564676748836	37.53837066657213	35.1700907887254	14.518973867953633
6	30.175114097522805	37.96015867151428	17.44189794054594	14.42282929041698
7	26.335159549385256	33.25014550301561	22.208469894243148	18.20622505335599
8	25.142291182114402	35.76702172869448	20.22195270979865	18.868734379392464
9	24.972298381733278	16.80410048406783	21.71043649312455	36.51316464107433
10-11	23.861461792219206	27.129504202777515	28.305482061415532	20.703551943587755
12-13	24.73321989975558	24.68303909210167	28.851569258084343	21.732171750058413
14-15	21.8925666651578	26.489474485289797	26.94812590742563	24.66983294212677
16-17	20.65500454930395	29.76848137334338	27.68565313165119	21.890860945701483
18-19	20.553881235260175	27.891606003176822	30.880398352318434	20.674114409244574
20-21	21.58571112776886	27.036716274173532	30.822534077297004	20.555038520760604
22-23	21.323080565089324	26.89235589488273	30.929670881187942	20.85489265884
24-25	21.13057564406234	26.93665502593711	30.897266887175945	21.0355024428246
26-27	20.936364093151813	27.049431765494695	30.89134129243008	21.12286284892341
28-29	20.92937166208806	27.020106467460973	31.062956258512102	20.987565611938862
30-31	21.21596197295196	27.25312793945885	30.695564316769598	20.83534577081959
32-33	20.935220265920847	27.13094777169006	30.992854565646454	20.940977396742635
34-35	21.14852089391076	26.967740593429635	30.82427733013942	21.059461182520185
36-37	21.401424398713274	27.077602145460826	30.45527172111353	21.06570173471237
38-39	21.209666586047327	27.165189613904655	30.602290927218608	21.02285287282941
40-41	21.174713634097674	27.05637547849727	30.577094331513077	21.191816555891982
42-43	21.158797296170896	27.180900863261737	30.36329682219656	21.29700501837081
44-45	21.369350011331143	27.271637906168756	30.1518138838809	21.2071981986192
46-47	21.47446522789222	27.416591577393323	29.70289697859124	21.406046216123215
48-49	21.546443991263814	27.467482841045076	29.37921594346821	21.606857224222896
50-51	21.60552414851987	27.56917014865552	29.119588463416374	21.705717239408237
52-53	21.594295549329	27.702001620492684	29.051528356644337	21.652174473533982
54-55	21.642403468106313	27.866446030430165	28.924922787815806	21.56622771364772
56-57	21.519782477203307	27.67090140381661	28.95194320687328	21.857372912106804
58-59	21.42349338331645	27.387158938840265	29.26918735684972	21.920160320993567
60-61	21.591849135676195	27.622200193105538	29.05277353724606	21.733177133972205
62-63	21.378527724825048	27.631568345985592	29.164876414360357	21.825027514829003
64-65	21.365753636769686	27.485852917216434	29.239528653729778	21.9088647922841
66-67	21.30651233849458	27.46951907755849	29.18267517237328	22.041293411573644
68-69	21.602993004427862	27.631096295250234	28.917287474741144	21.84862322558076
70-71	21.559928564721336	27.446893413565938	28.90110321536395	22.09207480634878
72-73	21.448213890971108	27.575535218907877	28.986888834231156	21.989362055889856
74-75	21.547755093191515	27.65957758493267	28.754582081499564	22.038085240376258
76-77	21.585388845730765	27.595099203538542	28.660028926277835	22.159483024452857
78-79	21.579796752520046	27.660096229169834	28.765173739217907	21.99493327909221
80-81	21.519894666248284	27.607517217960766	28.73972613576135	22.1328619800296
82-83	21.407192438005715	27.59563772116776	28.880778583461346	22.11639125736518
84-85	21.445203483751637	27.542984733060383	28.67116230577563	22.34064947741235
86-87	21.49225720782211	27.621514472702813	28.65699971573249	22.22922860374259
88-89	21.39449216582949	27.523335071216337	28.674117345630062	22.40805541732411
90-91	21.332872958021493	27.494374892923553	28.62751196124937	22.545240187805586
92-93	21.327998844464275	27.55820124926186	28.606540818552322	22.50725908772154
94-95	21.20649189041073	27.387077614343003	28.74442200906044	22.66200848618583
96-97	21.248365517106	27.416156118129862	28.694700422687603	22.640777942076536
98-99	21.181624259822154	27.445596383583503	28.626416402816425	22.746362953777922
100	21.213852941447914	27.37926125439059	28.572068956778153	22.834816847383344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1355.0
1	1012.0
2	1897.0
3	2975.5
4	1850.5
5	1006.0
6	2104.5
7	5474.5
8	9190.5
9	10537.5
10	8402.5
11	5526.0
12	5079.5
13	5577.0
14	6239.0
15	7002.0
16	7735.5
17	8590.0
18	9601.0
19	10826.0
20	12462.5
21	14806.5
22	18387.5
23	24054.5
24	30717.5
25	38469.0
26	49970.0
27	64716.5
28	83324.0
29	102635.5
30	118599.0
31	135285.5
32	151768.0
33	166432.0
34	181284.5
35	195744.5
36	206693.5
37	216450.0
38	226717.0
39	233386.0
40	237758.5
41	239837.0
42	237251.5
43	235132.5
44	233179.0
45	228933.5
46	224961.5
47	218433.0
48	211443.0
49	209674.0
50	207752.5
51	199967.0
52	188485.0
53	179546.5
54	173554.0
55	164746.0
56	151629.0
57	136128.5
58	123295.0
59	113079.0
60	99604.5
61	84402.5
62	71827.5
63	59754.0
64	48339.0
65	39086.0
66	31298.0
67	24517.5
68	18712.5
69	14297.0
70	10611.5
71	7783.0
72	5810.0
73	4063.0
74	2870.0
75	1997.5
76	1240.0
77	750.0
78	524.0
79	380.5
80	238.0
81	104.5
82	45.0
83	21.5
84	14.5
85	7.0
86	6.5
87	5.5
88	3.5
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059036209705406385
2	3.369312216437585E-4
3	1.4649183549728632E-5
4	0.12142708244370062
5	0.002065534880511737
6	0.002065534880511737
7	0.0
8	0.0
9	4.3947550649185894E-5
10-11	0.0
12-13	5.859673419891453E-5
14-15	2.9298367099457264E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.1719346839782906E-4
30-31	2.9298367099457265E-4
32-33	2.9298367099457264E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.0508856969620083E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.4649183549728632E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.538164272721506E-4
80-81	2.490361203453867E-4
82-83	3.5890499696835147E-4
84-85	0.0
86-87	3.662295887432158E-5
88-89	8.789510129837179E-5
90-91	6.592132597377883E-5
92-93	7.031608103869743E-4
94-95	7.104854021618386E-4
96-97	1.4649183549728632E-5
98-99	5.127214242405021E-5
100	0.0010547412155804614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6826319.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.97237785146881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07396736719849	40.17049745215537
2	15.38805425881223	16.918358662286597
3	5.077336826150501	8.373398354589682
4	2.1895927644597495	4.81468483154894
5	1.1654570253902508	3.2033971984700873
6	0.6874956237127481	2.2675961518781045
7	0.48355580724161384	1.8607548783570609
8	0.33932632695318815	1.4922860048177364
9	0.24048198322536757	1.1897879803501943
>10	1.2543799688146977	12.233167547279766
>50	0.06913891724071673	2.5803152826593245
>100	0.029240649893137658	2.837366015275801
>500	0.0013061423398466109	0.47674090648849665
>1k	5.597243980117042E-4	0.5563250587221735
>5k	2.665354235550714E-5	0.10177338945651325
>10k+	7.996062706652143E-5	0.923550285664115
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25257	0.36999442891549605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23528	0.3446659905580152	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14247	0.20870691803298383	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6946	0.10175322893641507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9298367099457264E-5	0.0	0.0	0.0
3	0.0	2.9298367099457264E-5	0.0	2.9298367099457264E-5	0.0
4	0.0	2.9298367099457264E-5	0.0	4.3947550649185894E-5	0.0
5	0.0	4.3947550649185894E-5	0.0	7.324591774864316E-5	0.0
6	0.0	4.3947550649185894E-5	0.0	7.324591774864316E-5	0.0
7	0.0	4.3947550649185894E-5	0.0	7.324591774864316E-5	0.0
8	0.0	4.3947550649185894E-5	0.0	8.789510129837179E-5	0.0
9	2.9298367099457264E-5	4.3947550649185894E-5	0.0	1.3184265194755767E-4	0.0
10-11	2.9298367099457264E-5	5.859673419891453E-5	0.0	1.39167243722422E-4	0.0
12-13	2.9298367099457264E-5	7.324591774864316E-5	0.0	1.904393861464722E-4	0.0
14-15	2.9298367099457264E-5	8.789510129837179E-5	0.0	1.9776397792133652E-4	0.0
16-17	2.9298367099457264E-5	1.0254428484810042E-4	0.0	2.2706234502079377E-4	0.0
18-19	2.9298367099457264E-5	1.0986887662296473E-4	0.0	2.56360712120251E-4	0.0
20-21	2.9298367099457264E-5	1.2451806017269336E-4	0.0	3.0030826276943696E-4	0.0
22-23	2.9298367099457264E-5	1.3184265194755767E-4	0.0	4.760984653661805E-4	0.0
24-25	2.9298367099457264E-5	1.3184265194755767E-4	0.0	9.30223155407768E-4	0.0
26-27	2.9298367099457264E-5	1.3184265194755767E-4	0.0	0.0015235150891717776	0.0
28-29	2.9298367099457264E-5	1.3184265194755767E-4	0.0	0.003779489355829987	0.0
30-31	2.9298367099457264E-5	1.3184265194755767E-4	0.0	0.010298376035459227	0.0
32-33	2.9298367099457264E-5	1.3184265194755767E-4	0.0	0.025445631825878633	0.0
34-35	2.9298367099457264E-5	1.4649183549728632E-4	0.0	0.047243616947874836	0.0
36-37	2.9298367099457264E-5	1.4649183549728632E-4	0.0	0.08194753277718198	0.0
38-39	2.9298367099457264E-5	1.5381642727215064E-4	0.0	0.12923509727570598	0.0
40-41	2.9298367099457264E-5	1.6114101904701495E-4	0.0	0.1791741639967309	0.0
42-43	2.9298367099457264E-5	1.904393861464722E-4	0.0	0.23054152611385434	0.0
44-45	2.9298367099457264E-5	2.1241316147106514E-4	0.0	0.2946024057768176	0.0
46-47	2.9298367099457264E-5	2.343869367956581E-4	0.0	0.37206728838778264	0.0
48-49	2.9298367099457264E-5	2.343869367956581E-4	0.0	0.4543590769783832	0.0
50-51	2.9298367099457264E-5	2.343869367956581E-4	0.0	0.5378960461707107	0.0
52-53	2.9298367099457264E-5	2.343869367956581E-4	0.0	0.6196531395617462	0.0
54-55	2.9298367099457264E-5	2.343869367956581E-4	0.0	0.7125289632670258	0.0
56-57	2.9298367099457264E-5	2.6368530389511534E-4	0.0	0.8238993812038378	0.0
58-59	2.9298367099457264E-5	2.6368530389511534E-4	0.0	0.9455388768090094	0.0
60-61	2.9298367099457264E-5	2.6368530389511534E-4	0.0	1.072364183390785	0.0
62-63	2.9298367099457264E-5	2.6368530389511534E-4	0.0	1.2000244934348951	0.0
64-65	2.9298367099457264E-5	2.6368530389511534E-4	0.0	1.3359322938175024	0.0
66-67	2.9298367099457264E-5	2.6368530389511534E-4	0.0	1.4933524202428865	0.0
68-69	2.9298367099457264E-5	2.6368530389511534E-4	0.0	1.6681025308075994	0.0
70-71	2.9298367099457264E-5	2.6368530389511534E-4	0.0	1.8567180936021304	7.324591774864316E-6
72-73	2.9298367099457264E-5	2.8565907921970833E-4	0.0	2.053185618779316	1.4649183549728632E-5
74-75	2.9298367099457264E-5	3.0030826276943696E-4	0.0	2.2527807446443684	1.4649183549728632E-5
76-77	2.9298367099457264E-5	3.0763285454430127E-4	0.0	2.469654582506326	1.4649183549728632E-5
78-79	2.9298367099457264E-5	3.0763285454430127E-4	0.0	2.710428563329666	1.4649183549728632E-5
80-81	2.9298367099457264E-5	3.0763285454430127E-4	0.0	2.9711767059230603	1.4649183549728632E-5
82-83	2.9298367099457264E-5	3.149574463191656E-4	0.0	3.240780279972266	1.4649183549728632E-5
84-85	2.9298367099457264E-5	3.222820380940299E-4	0.0	3.514954399288987	1.4649183549728632E-5
86-87	2.9298367099457264E-5	3.5158040519348715E-4	0.0	3.804422266231625	1.4649183549728632E-5
88	2.9298367099457264E-5	3.9552795584267303E-4	0.0	4.038340429153692	1.4649183549728632E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13975	0.0	58.384064	1
GTATCAA	25800	0.0	40.748962	1
ATCAACG	32295	0.0	32.215054	3
TCAACGC	32615	0.0	31.826843	4
TATCAAC	33310	0.0	31.275799	2
CAACGCA	33710	0.0	30.812695	5
GTGGTAT	5435	0.0	30.405708	1
AACGCAG	35340	0.0	29.483957	6
TGGTATC	6065	0.0	26.145956	2
ACGCAGA	40175	0.0	25.782581	7
CGCAGAG	40295	0.0	25.624155	8
GCAGAGT	43495	0.0	23.436401	9
GTACATG	22000	0.0	23.155394	1
TACATGG	22655	0.0	21.933367	2
GAGTACT	30375	0.0	21.344015	12-13
CAGAGTA	42215	0.0	20.868408	10-11
ACATGGG	23015	0.0	20.752026	3
AGAGTAC	40570	0.0	20.718323	10-11
TATAACG	700	0.0	20.166412	2
AGTACTT	31485	0.0	19.688461	12-13
>>END_MODULE