FastQCFastQC Report
Wed 25 May 2016
SRR2049495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6829172
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.040.580651
GTATCAA93950.034.1635251
TCAACGC96200.031.9740584
ATCAACG96750.031.6864973
CAACGCA105400.029.2317125
TATCAAC112700.027.6561532
AACGCAG116650.026.4561946
GTACATG157300.023.4312631
ACGCAGA136450.022.2790627
TACATGG157700.022.0361862
CGCAGAG139100.021.8230728
TCTGTCG19950.021.5427558
GTGGTAT38600.020.9537491
ACATGGG159900.020.8367943
TACCTGG45700.020.7182882
CGCCGTA16250.018.65544394
CTGTCGC23000.018.4893029
TCGCCGT21100.017.79835194
GCAGAGT162550.017.7842039
TGGTATC42050.017.5264872
Produced by FastQC (version 0.11.5)