##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6829172 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.02577677059532 31.0 31.0 34.0 28.0 34.0 2 31.1115707731479 31.0 31.0 34.0 28.0 34.0 3 31.261952107810433 31.0 31.0 34.0 28.0 34.0 4 34.83158587893232 37.0 35.0 37.0 32.0 37.0 5 34.849236920669156 37.0 35.0 37.0 32.0 37.0 6 34.85879752333079 37.0 35.0 37.0 32.0 37.0 7 30.83535309990728 37.0 33.0 37.0 2.0 37.0 8 32.63988533309748 37.0 33.0 37.0 17.0 37.0 9 35.46053152563737 39.0 34.0 39.0 29.0 39.0 10-11 36.00926063950359 39.0 36.0 39.0 31.5 39.0 12-13 36.18206065098375 39.0 37.0 39.0 32.0 39.0 14-15 37.39412230355305 40.0 38.0 41.0 32.0 41.0 16-17 37.41531096302744 40.0 38.0 41.0 32.0 41.0 18-19 37.416151840955244 40.0 38.0 41.0 32.0 41.0 20-21 37.48791991181361 40.0 38.0 41.0 32.0 41.0 22-23 37.447543421076524 40.0 38.0 41.0 32.0 41.0 24-25 37.421080256874475 40.0 38.0 41.0 31.0 41.0 26-27 37.44240839152975 40.0 38.0 41.0 32.0 41.0 28-29 37.41760370071218 40.0 38.0 41.0 32.0 41.0 30-31 37.33053736236252 40.0 37.0 41.0 31.0 41.0 32-33 37.2654329836765 40.0 37.0 41.0 31.0 41.0 34-35 37.19897551269759 40.0 37.0 41.0 31.0 41.0 36-37 37.01966263260026 40.0 37.0 41.0 30.0 41.0 38-39 36.90043609972044 40.0 37.0 41.0 30.0 41.0 40-41 36.82195176809136 40.0 37.0 41.0 30.0 41.0 42-43 36.70742983190348 40.0 36.5 41.0 30.0 41.0 44-45 36.59767340755219 40.0 36.0 41.0 30.0 41.0 46-47 36.4410867086083 40.0 36.0 41.0 30.0 41.0 48-49 36.3572846019986 40.0 36.0 41.0 30.0 41.0 50-51 36.21627922096559 40.0 35.5 41.0 29.0 41.0 52-53 36.095114751832284 39.0 35.0 41.0 29.0 41.0 54-55 35.96629811051764 39.0 35.0 41.0 29.0 41.0 56-57 35.763660587257135 39.0 35.0 41.0 28.0 41.0 58-59 35.576421709688965 39.0 35.0 41.0 28.0 41.0 60-61 35.344110823391176 39.0 35.0 40.5 28.0 41.0 62-63 35.08974191893248 38.0 34.0 40.0 28.0 41.0 64-65 34.71786704156814 37.5 34.0 40.0 27.0 41.0 66-67 31.205172750078635 36.0 31.0 40.0 6.5 41.0 68-69 31.556226069573295 36.0 30.5 39.0 11.0 41.0 70-71 31.701261148496478 35.5 30.0 39.0 15.5 41.0 72-73 32.92957499093595 35.0 32.0 39.0 24.0 40.5 74-75 33.002323780979594 35.0 33.0 38.5 25.0 40.0 76-77 32.69291511474597 35.0 33.0 37.0 24.5 39.5 78-79 29.315406025796392 35.0 29.0 36.5 6.5 39.0 80-81 31.083441228307034 35.0 30.0 36.0 19.5 39.0 82-83 31.470705160157046 35.0 32.0 36.0 21.5 37.5 84-85 29.614818458225976 35.0 30.0 35.5 11.0 37.0 86-87 29.231788934295402 34.0 28.0 35.0 11.0 37.0 88-89 30.451142246819966 34.0 30.5 35.0 18.0 36.0 90-91 30.570217443637382 35.0 31.5 35.0 17.5 36.0 92-93 30.384564629504133 35.0 32.0 35.0 9.0 36.0 94-95 30.1325599208806 35.0 31.0 35.0 2.0 36.0 96-97 29.89009472890711 35.0 31.0 35.0 2.0 35.0 98-99 29.64847085415333 34.5 31.0 35.0 2.0 35.0 100 29.397847938227358 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32429447436461345 1101 2 0.30836105789353496 1101 3 0.2922199040744111 1101 4 0.3231124745155469 1101 5 0.3145236780846332 1101 6 0.31483681792948914 1101 7 -12.198387877059652 1101 8 -6.602909182348849 1101 9 -2.223667226626283 1101 10-11 -0.19140830627681993 1101 12-13 0.2967739588306628 1101 14-15 0.5051467049916809 1101 16-17 0.5405980227381164 1101 18-19 0.5166785822904885 1101 20-21 0.5048064301340034 1101 22-23 0.4677612507025444 1101 24-25 0.5039584133419766 1101 26-27 0.48979557458537926 1101 28-29 0.42005819220909046 1101 30-31 0.47339841781146674 1101 32-33 0.51540768192136 1101 34-35 0.471179140360924 1101 36-37 0.5614387906509819 1101 38-39 0.581035477928026 1101 40-41 0.508768263323681 1101 42-43 0.5278893957133235 1101 44-45 0.32245092244808404 1101 46-47 0.37086151084128716 1101 48-49 0.32786570309983176 1101 50-51 0.3902134383203446 1101 52-53 0.4427531022034543 1101 54-55 0.4168693510099786 1101 56-57 0.3584170121305803 1101 58-59 0.3506068770263582 1101 60-61 0.3580974531988801 1101 62-63 0.3597904842654742 1101 64-65 0.24769940650040922 1101 66-67 -10.297864824108053 1101 68-69 -8.16244686497032 1101 70-71 -6.6769354457886365 1101 72-73 -1.5582478352684106 1101 74-75 -0.2822440285135812 1101 76-77 0.0195629486806439 1101 78-79 3.1477004427012076 1101 80-81 1.027395253352534 1101 82-83 0.34860568323280106 1101 84-85 -5.382872364147602 1101 86-87 -5.899786046687403 1101 88-89 -1.3920613807702829 1101 90-91 -0.12221053531628101 1101 92-93 0.26131000154616046 1101 94-95 0.2537755893180851 1101 96-97 0.3405530523546467 1101 98-99 0.37210667052389823 1101 100 0.3948960675483555 1102 1 0.192793344037554 1102 2 0.2142654011956786 1102 3 0.23435751701962815 1102 4 0.1740542037643067 1102 5 0.21238195910096636 1102 6 0.23829741512478364 1102 7 -12.258742279502744 1102 8 -6.677842750846203 1102 9 -2.367414686598245 1102 10-11 -0.35476142324991855 1102 12-13 0.19350176114357964 1102 14-15 0.34020928708329023 1102 16-17 0.39785808194905314 1102 18-19 0.3743122793348519 1102 20-21 0.34256541967527454 1102 22-23 0.3379628431922299 1102 24-25 0.40717143508297937 1102 26-27 0.387438638529936 1102 28-29 0.33812715265815285 1102 30-31 0.3581295072881119 1102 32-33 0.3635370314642117 1102 34-35 0.3403532083951788 1102 36-37 0.49458837876399997 1102 38-39 0.4813419960307499 1102 40-41 0.4774406597803633 1102 42-43 0.43653968721872616 1102 44-45 0.4284515126911117 1102 46-47 0.4363915980712747 1102 48-49 0.3435361771839638 1102 50-51 0.3979007121674414 1102 52-53 0.3982131301525129 1102 54-55 0.39196366158046914 1102 56-57 0.42231732871548644 1102 58-59 0.3725627744407163 1102 60-61 0.33573509297332293 1102 62-63 0.3661246901319757 1102 64-65 0.2513781603177492 1102 66-67 -10.281669616974746 1102 68-69 -6.730122823881942 1102 70-71 -6.278807006106575 1102 72-73 -1.1245141865166666 1102 74-75 -0.21972778516548175 1102 76-77 -0.0434612302866384 1102 78-79 3.112088094723781 1102 80-81 1.02008173398767 1102 82-83 0.358807054089727 1102 84-85 -5.455949828295587 1102 86-87 -5.917789637138064 1102 88-89 -1.3980649222284391 1102 90-91 -0.10667313420531954 1102 92-93 0.2628878867963813 1102 94-95 0.2897128975765888 1102 96-97 0.39237212070385397 1102 98-99 0.36510207222743674 1102 100 0.4907830535061741 1103 1 0.20338307863675453 1103 2 0.2533818556855536 1103 3 0.23733666795284591 1103 4 0.24129927694731634 1103 5 0.258433133265747 1103 6 0.2606448006751805 1103 7 -12.24841691178958 1103 8 -6.691151450094594 1103 9 -2.3211797457560124 1103 10-11 -0.3234036716046518 1103 12-13 0.22650415262774004 1103 14-15 0.38995289930323196 1103 16-17 0.39002815153584436 1103 18-19 0.42442070527076226 1103 20-21 0.4000447639051501 1103 22-23 0.4455839008395799 1103 24-25 0.5189249503848856 1103 26-27 0.49499860544473506 1103 28-29 0.4564810069034806 1103 30-31 0.4300370305736152 1103 32-33 0.4867807965233695 1103 34-35 0.44676798255276395 1103 36-37 0.5187264330309134 1103 38-39 0.532825637739677 1103 40-41 0.5566089447445748 1103 42-43 0.5024791089470639 1103 44-45 0.4816667552559366 1103 46-47 0.4959856975785968 1103 48-49 0.48993463350790734 1103 50-51 0.5000078704369457 1103 52-53 0.4144516896878798 1103 54-55 0.45257396204438294 1103 56-57 0.45387179341861383 1103 58-59 0.467599298517392 1103 60-61 0.4394656284500087 1103 62-63 0.40349286631063563 1103 64-65 0.286454534971611 1103 66-67 -10.341210020470267 1103 68-69 -8.20948914800896 1103 70-71 -6.701705421973081 1103 72-73 -1.5401144400499511 1103 74-75 -0.30936775891309765 1103 76-77 0.013463217694088314 1103 78-79 3.1522517001109094 1103 80-81 1.0232688136798238 1103 82-83 0.31690762229197844 1103 84-85 -5.51109594707863 1103 86-87 -5.961849743406269 1103 88-89 -1.3922455916641923 1103 90-91 -0.10948106026710036 1103 92-93 0.3170292900401712 1103 94-95 0.377619472359914 1103 96-97 0.4946618544040007 1103 98-99 0.5120472658837087 1103 100 0.5792556952870171 1104 1 0.22225604697672452 1104 2 0.23794331895464182 1104 3 0.2878697317295149 1104 4 0.2160229980071975 1104 5 0.2454398425375217 1104 6 0.3047283518742532 1104 7 -12.154532103135153 1104 8 -6.614173582364934 1104 9 -2.3248301673786713 1104 10-11 -0.3321754893461417 1104 12-13 0.24242841303223628 1104 14-15 0.41746657352310734 1104 16-17 0.38916955949964915 1104 18-19 0.4281452954524525 1104 20-21 0.4541649595764383 1104 22-23 0.45039606737723403 1104 24-25 0.5003578480205917 1104 26-27 0.4420220303550124 1104 28-29 0.40859483093284155 1104 30-31 0.42711215346579934 1104 32-33 0.42312507542389 1104 34-35 0.39974602049355923 1104 36-37 0.44921188232737563 1104 38-39 0.5052080831121799 1104 40-41 0.5212893734515447 1104 42-43 0.4077294094291588 1104 44-45 0.4064314361458159 1104 46-47 0.4157499081983431 1104 48-49 0.3967723706822923 1104 50-51 0.4478486409065283 1104 52-53 0.46028982127145923 1104 54-55 0.4229101506063273 1104 56-57 0.41019717414490486 1104 58-59 0.41298684235426464 1104 60-61 0.37951441638428207 1104 62-63 0.2800341644897344 1104 64-65 0.18139492976666105 1104 66-67 -10.359279774624977 1104 68-69 -8.286302077429262 1104 70-71 -6.790675357770951 1104 72-73 -1.6833249050347732 1104 74-75 -0.45352445478122405 1104 76-77 -0.1584095399022445 1104 78-79 3.0471561900038466 1104 80-81 0.939611073993948 1104 82-83 0.1999533454950857 1104 84-85 -5.537058195282082 1104 86-87 -6.000506989763338 1104 88-89 -1.4817910371900282 1104 90-91 -0.1844776585830452 1104 92-93 0.16767141290764087 1104 94-95 0.1813532130294 1104 96-97 0.2834271486547486 1104 98-99 0.32307792030341176 1104 100 0.41344184364647774 1105 1 0.13100062075992014 1105 2 0.11270819926486908 1105 3 0.10562820481371205 1105 4 0.15836709834184148 1105 5 0.15086729386927544 1105 6 0.1721966018666592 1105 7 -12.315575889901176 1105 8 -6.782134857533883 1105 9 -2.4940381118915624 1105 10-11 -0.4367546904079376 1105 12-13 0.12847011486441318 1105 14-15 0.3149537305668204 1105 16-17 0.29510059558609925 1105 18-19 0.3149248814725709 1105 20-21 0.3212442195509979 1105 22-23 0.298682699951172 1105 24-25 0.33973183687541564 1105 26-27 0.3214156597438276 1105 28-29 0.3074731028633053 1105 30-31 0.30131261688737965 1105 32-33 0.34291326664603616 1105 34-35 0.4011440290523822 1105 36-37 0.49063220950124986 1105 38-39 0.47754474682271564 1105 40-41 0.4947401239076399 1105 42-43 0.4025396199543678 1105 44-45 0.3290825965121087 1105 46-47 0.3552227036731139 1105 48-49 0.3255526185150259 1105 50-51 0.3944321134121509 1105 52-53 0.3333479951913887 1105 54-55 0.3098731173477489 1105 56-57 0.2458471380370426 1105 58-59 0.26586699191599195 1105 60-61 0.24516589236218067 1105 62-63 0.1738455974961397 1105 64-65 -0.006911531259156334 1105 66-67 -10.441482990410446 1105 68-69 -8.394767043471244 1105 70-71 -6.8476851967593895 1105 72-73 -1.7827893682105156 1105 74-75 -0.575479608510058 1105 76-77 -0.3015117009769881 1105 78-79 2.826162393012382 1105 80-81 0.7184104869384846 1105 82-83 0.05109902304562297 1105 84-85 -5.609512869413329 1105 86-87 -6.124239600462971 1105 88-89 -1.598096088047896 1105 90-91 -0.3550886261480066 1105 92-93 0.06424720339049728 1105 94-95 0.10262627709811412 1105 96-97 0.14401734285240408 1105 98-99 0.18857041473754776 1105 100 0.26277664097065667 1106 1 0.0794663296710425 1106 2 0.13235268649441778 1106 3 0.14225549442801722 1106 4 0.06579771637873932 1106 5 0.09160624064488587 1106 6 0.14885959428054463 1106 7 -12.338222191977223 1106 8 -6.775017493779146 1106 9 -2.5937366823473056 1106 10-11 -0.4988138132293969 1106 12-13 0.08990979406486588 1106 14-15 0.2239728323892436 1106 16-17 0.26870659908076533 1106 18-19 0.27110456810640216 1106 20-21 0.2660317646490853 1106 22-23 0.26188718270353206 1106 24-25 0.3323257797643393 1106 26-27 0.3425907898509095 1106 28-29 0.32762996485949714 1106 30-31 0.26925073222766116 1106 32-33 0.28902899697623496 1106 34-35 0.29652874511568683 1106 36-37 0.33672941183674254 1106 38-39 0.2961856407690391 1106 40-41 0.26671444860153315 1106 42-43 0.2531795374435504 1106 44-45 0.20196028874379834 1106 46-47 0.26093650190286866 1106 48-49 0.22443464548729253 1106 50-51 0.25615949735912835 1106 52-53 0.2555667886254156 1106 54-55 0.20564631366650588 1106 56-57 0.2066353764158464 1106 58-59 0.183586969294673 1106 60-61 0.1879027858193183 1106 62-63 0.09602763393494484 1106 64-65 -0.02889204079250618 1106 66-67 -10.503774851272635 1106 68-69 -8.453928251926662 1106 70-71 -6.946771306555171 1106 72-73 -1.807718216033031 1106 74-75 -0.583492035480127 1106 76-77 -0.32585275033760297 1106 78-79 2.8690583851351334 1106 80-81 0.7151160966336931 1106 82-83 0.03546403341469784 1106 84-85 -5.653051694218604 1106 86-87 -6.103591780364496 1106 88-89 -1.5879373939889234 1106 90-91 -0.31717178424422343 1106 92-93 0.04970035970577058 1106 94-95 0.11160519313711958 1106 96-97 0.21623029384354453 1106 98-99 0.1816895168059176 1106 100 0.2676221600773765 1107 1 0.03432718610686791 1107 2 0.07414597496084951 1107 3 0.036544710843092076 1107 4 0.041066075300747684 1107 5 0.06955207658003815 1107 6 0.08037049878804936 1107 7 -12.392571274980991 1107 8 -6.843385005912701 1107 9 -2.627516371979347 1107 10-11 -0.6067012032509709 1107 12-13 0.016098122723192887 1107 14-15 0.1336780257827641 1107 16-17 0.1905588951230328 1107 18-19 0.2085918894069536 1107 20-21 0.17143726827841732 1107 22-23 0.19536192276953557 1107 24-25 0.2816367523320906 1107 26-27 0.18058096406532087 1107 28-29 0.2191102092156214 1107 30-31 0.19613254857732443 1107 32-33 0.1984536896313429 1107 34-35 0.16735171247650982 1107 36-37 0.2542179225397092 1107 38-39 0.24284887138425404 1107 40-41 0.22973559683742195 1107 42-43 0.27414539829006657 1107 44-45 0.22385983049347402 1107 46-47 0.30616022519500063 1107 48-49 0.25566240613987645 1107 50-51 0.24350954179652717 1107 52-53 0.2505881483106194 1107 54-55 0.14170403933083975 1107 56-57 0.169783724633227 1107 58-59 0.14109127261873056 1107 60-61 0.10227638372860781 1107 62-63 0.036552556871583874 1107 64-65 -0.08966224494022157 1107 66-67 -10.543090878800694 1107 68-69 -8.485482107077274 1107 70-71 -6.949129957640665 1107 72-73 -1.840125797812707 1107 74-75 -0.5410753019095722 1107 76-77 -0.3342687636589119 1107 78-79 2.812514524268792 1107 80-81 0.7221325797689566 1107 82-83 0.07570449093816123 1107 84-85 -5.630009995751941 1107 86-87 -6.179699328774831 1107 88-89 -1.656627696724879 1107 90-91 -0.36408412858944317 1107 92-93 0.03273882035471942 1107 94-95 0.1167361430401499 1107 96-97 0.16575073339381774 1107 98-99 0.22851629025448972 1107 100 0.25359646237721734 1108 1 0.029342259556774053 1108 2 0.05841523831603723 1108 3 0.05678498040967028 1108 4 0.013203848546993413 1108 5 -0.0051359941022610656 1108 6 0.014158186945493867 1108 7 -12.329828724151 1108 8 -6.829170589395936 1108 9 -2.712653883801046 1108 10-11 -0.6613410723045305 1108 12-13 -0.05503239296546525 1108 14-15 0.1005218661695082 1108 16-17 0.03513196876946978 1108 18-19 0.1185193419741637 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-0.5590321811860903 2216 6 -0.8380950970468461 2216 7 3.3362061514825534 2216 8 1.395148698330516 2216 9 0.12633685228959024 2216 10-11 -0.5615779617844154 2216 12-13 -0.929777697167907 2216 14-15 -1.1468162798411257 2216 16-17 -1.271911447513908 2216 18-19 -1.793020488654797 2216 20-21 -1.329652401296748 2216 22-23 -1.1309963479199752 2216 24-25 -1.1483381739000365 2216 26-27 -1.5207166115416655 2216 28-29 -1.7278936460385879 2216 30-31 -1.695253702748225 2216 32-33 -2.622615786141779 2216 34-35 -1.6760829877344463 2216 36-37 -1.3442735509176629 2216 38-39 -1.5577641286498505 2216 40-41 -1.5704519286382705 2216 42-43 -1.1812433100698527 2216 44-45 -1.288913743963768 2216 46-47 -1.4751330631874353 2216 48-49 -1.5276981689328295 2216 50-51 -2.0782616828069465 2216 52-53 -1.74112727741565 2216 54-55 -1.330548361271319 2216 56-57 -1.3469888234884309 2216 58-59 -1.7383008996237663 2216 60-61 -1.934338178544138 2216 62-63 -1.812460407110855 2216 64-65 -1.4736780021144185 2216 66-67 1.9488910775053405 2216 68-69 1.1524733496339366 2216 70-71 0.8392603925313935 2216 72-73 -0.9591324121557463 2216 74-75 -1.8059742192229784 2216 76-77 -1.4027864070495255 2216 78-79 1.1247580418715835 2216 80-81 -0.32939632732980684 2216 82-83 -1.279386710020617 2216 84-85 0.537447533929786 2216 86-87 0.45275175383056876 2216 88-89 -0.8520297295838546 2216 90-91 -1.1557830712364456 2216 92-93 -1.1496415798836743 2216 94-95 -1.0775301406994124 2216 96-97 -1.0641658930801192 2216 98-99 -1.0298492784604605 2216 100 -2.273301405865329 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83926.0 3 16823.0 4 9920.0 5 9161.0 6 9032.0 7 10741.0 8 12744.0 9 14620.0 10 16047.0 11 17596.0 12 20031.0 13 22321.0 14 24999.0 15 26463.0 16 27895.0 17 28468.0 18 27201.0 19 25921.0 20 25498.0 21 25660.0 22 27423.0 23 31292.0 24 37372.0 25 46663.0 26 57315.0 27 69995.0 28 86380.0 29 109896.0 30 134135.0 31 169214.0 32 226521.0 33 319790.0 34 487964.0 35 672231.0 36 753935.0 37 1262893.0 38 1410403.0 39 469363.0 40 1320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66938298473755 20.510964620005947 13.717147514525028 23.102504880731473 2 16.14353446495926 20.62104608672961 39.538576252959075 23.696843195352052 3 18.411788436630783 25.130599869821125 30.45566267124416 26.001949022303933 4 12.947002474880353 17.28504995667751 38.49690226280005 31.271045305642087 5 12.982151198632897 37.29964577692586 35.08779431339443 14.63040871104681 6 30.447312486134287 37.85550956633541 18.004318414619057 13.692859532911255 7 26.91690645891059 32.32408834912005 22.643897294984463 18.115107896984888 8 24.627597606269106 35.89430753830772 21.08731483113912 18.390780024284055 9 24.411451344321097 17.465045542856437 22.13360565526831 35.98989745755416 10-11 23.465465505920776 27.192322876038265 29.009168314987527 20.33304330305343 12-13 24.055566326342344 24.75529976401239 28.89441794700734 22.29471596263793 14-15 21.682599296078646 27.262638867493745 26.07098635090755 24.98377548552006 16-17 21.085304940183704 30.314120669909723 26.71408002068776 21.886494369218816 18-19 20.95738692772711 29.277875853763824 28.635038039750647 21.129699178758422 20-21 22.222380855422948 28.752880199303828 27.999560415198243 21.025178530074985 22-23 21.836051281180207 28.788658420083724 27.994499186724248 21.38079111201182 24-25 21.622474876895765 28.70515634984739 28.144832199276866 21.52753657397998 26-27 21.378717451059305 28.61103604492413 28.207406520144467 21.802839983872097 28-29 21.467631800751246 28.626237558521005 28.296248798536634 21.60988184219112 30-31 21.890625979254878 29.19329751835215 27.700759330706564 21.215317171686408 32-33 21.309615860374258 28.91234740734618 28.260181818567865 21.5178549137117 34-35 21.388661209437316 28.40235650977956 28.372587141844157 21.836395138938972 36-37 21.974313419392608 28.818777218721635 27.746113765818357 21.4607955960674 38-39 21.743472103460867 28.82067026724984 28.00143472747756 21.434422901811732 40-41 21.441552504461743 28.53867203813288 28.26024150511951 21.75953395228587 42-43 21.344523368941854 28.555903783929264 28.23476670887286 21.86480613825602 44-45 21.589626490153112 28.687208273223487 28.117511894574506 21.605653342048896 46-47 21.407504546169733 28.99594902725476 27.93678318249966 21.65976324407585 48-49 21.25512153938935 28.51370311285189 28.326556109286937 21.904619238471817 50-51 21.239930934268287 28.36953344023298 28.319541834400535 22.0709937910982 52-53 21.197219861022337 28.40258421429884 28.322083771711142 22.07811215296768 54-55 21.32687025045813 28.83105214261433 28.077648953298766 21.764428653628773 56-57 21.078443826514338 28.558538302022463 28.106265983596746 22.256751887866454 58-59 20.918072131257965 28.125421218387803 28.44651546917093 22.509991181183302 60-61 21.350434011227858 28.455374555247946 27.983078555461155 22.211112878063037 62-63 21.165980476227293 28.404857630859414 28.064420191043855 22.364741701869438 64-65 21.121910879276683 28.160034089374054 28.267837341848846 22.45021768950042 66-67 21.083128594268448 28.189021115392 27.99806777102698 22.729782519312575 68-69 21.72242570237829 28.335559171833186 27.72501027727101 22.217004848517515 70-71 21.49079473859547 28.033943600613565 27.91501652883485 22.560245131956115 72-73 21.23902422809647 28.07316305113335 28.242439258432565 22.445373462337617 74-75 21.29653432155727 28.0136782344991 28.147498192072383 22.542289251871242 76-77 21.416086412212355 28.094988590163627 27.853809997924948 22.635114999699066 78-79 21.670849862936066 28.149675193257938 27.89774714046803 22.281727803337958 80-81 21.457440640976845 27.871441171375405 28.056436339652475 22.61468184799527 82-83 21.42688425396444 27.81928236608614 28.170835999724698 22.58299738022472 84-85 21.606333382092632 27.71335004207238 27.966266234164948 22.714050341670035 86-87 21.828681967064277 27.767926275176517 27.90171780771668 22.501673950042523 88-89 21.690186672527904 27.599395493761975 28.011620073642806 22.698797760067315 90-91 21.7759272190359 27.407081506271446 27.924886019819944 22.892105254872707 92-93 22.01767515133091 27.507490581584847 27.69577834468141 22.779055922402833 94-95 22.00777317590951 27.183644905181225 27.91338507152502 22.895196847384245 96-97 22.023750208761946 27.176664994130505 27.930609542417972 22.868975254689573 98-99 22.17863057686508 27.160625531894016 27.768618423157694 22.89212546808321 100 22.36080828511426 27.19974683429376 27.634776544800648 22.80466833579133 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3367.0 1 2598.0 2 2172.0 3 2469.5 4 2257.5 5 1919.5 6 1732.5 7 1754.5 8 1859.0 9 1904.5 10 1706.5 11 1463.0 12 1428.0 13 1480.0 14 1618.0 15 1910.0 16 2244.5 17 2701.0 18 3509.0 19 4759.5 20 6573.5 21 9226.5 22 13287.5 23 19297.5 24 26986.0 25 36856.0 26 49618.5 27 65369.5 28 84876.5 29 104353.0 30 120890.5 31 137632.5 32 154205.0 33 169617.0 34 185269.5 35 199709.0 36 211168.5 37 221818.5 38 231123.5 39 236571.5 40 240556.0 41 241714.0 42 239900.0 43 239027.5 44 237140.5 45 233942.0 46 231277.5 47 226822.0 48 222301.5 49 219783.0 50 214777.0 51 205734.0 52 195131.5 53 187376.5 54 181992.0 55 174218.5 56 161410.0 57 144645.0 58 128084.5 59 112985.5 60 96727.0 61 79863.5 62 65055.5 63 51890.5 64 40656.0 65 31687.0 66 24484.5 67 18603.0 68 14062.5 69 10568.5 70 7710.5 71 5745.5 72 4448.0 73 3252.0 74 2311.0 75 1614.5 76 1091.5 77 749.5 78 531.0 79 406.0 80 291.5 81 189.5 82 138.0 83 111.0 84 91.5 85 68.0 86 50.0 87 42.5 88 32.5 89 20.5 90 20.0 91 21.0 92 17.0 93 15.5 94 15.0 95 17.5 96 20.5 97 17.5 98 16.0 99 17.0 100 36.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019065268820290367 2 0.00260646532258962 3 0.0015668078062757826 4 0.002269674859558377 5 0.0020793150326276744 6 0.0040561286199849705 7 11.602416808362712 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9035982693070257E-4 18-19 0.0 20-21 7.321531805027023E-6 22-23 0.0 24-25 0.0 26-27 1.976813587357296E-4 28-29 0.0 30-31 0.0 32-33 2.196459541508107E-5 34-35 6.58937862452432E-5 36-37 4.392919083016214E-5 38-39 7.321531805027023E-6 40-41 0.0 42-43 1.4643063610054046E-5 44-45 1.1714450888043237E-4 46-47 0.0010469790481188641 48-49 7.101885850876211E-4 50-51 3.733981220563782E-4 52-53 2.489320813709188E-4 54-55 3.880411856664322E-4 56-57 0.0014716278928104316 58-59 3.587550584463241E-4 60-61 0.0012593034704646477 62-63 0.0024014624320488633 64-65 9.444776028484859E-4 66-67 5.801391442476482 68-69 5.464469191872748 70-71 0.0028846835311806466 72-73 0.0014130556383702153 74-75 0.0032287955260169167 76-77 0.006076871398172428 78-79 5.379956750247321 80-81 0.005051856945468646 82-83 0.005007927754638483 84-85 5.801742876003122 86-87 0.003873090324859295 88-89 0.0018669906102818906 90-91 0.00356558598904816 92-93 0.0038511257294442137 94-95 0.0044368482738463754 96-97 0.0032434385896269705 98-99 0.005732759403336158 100 0.006486877179253941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6829172.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.79750270403918 #Duplication Level Percentage of deduplicated Percentage of total 1 92.59839670660975 82.22506381944872 2 5.295967169783033 9.405373181586233 3 1.1063601867524642 2.947260650243797 4 0.4262440620669945 1.5139763301589835 5 0.2180483916748775 0.9681076324680664 6 0.11300196511163178 0.6020575381537119 7 0.06674115902580494 0.4148513774345236 8 0.04435328795588533 0.31507689657566046 9 0.031514195948508274 0.2518543709957956 >10 0.09853766980856217 1.2903164809771053 >50 6.963677920066062E-4 0.04203343316503619 >100 1.2166917328849796E-4 0.013326285351320657 >500 1.716829717331324E-5 0.010702003440990634 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4643063610054044E-5 0.0 5 0.0 0.0 0.0 4.392919083016214E-5 0.0 6 0.0 0.0 0.0 4.392919083016214E-5 0.0 7 0.0 0.0 0.0 4.392919083016214E-5 0.0 8 0.0 0.0 0.0 4.392919083016214E-5 0.0 9 0.0 0.0 0.0 4.392919083016214E-5 0.0 10-11 0.0 0.0 0.0 5.1250722635189154E-5 0.0 12-13 0.0 0.0 0.0 1.0250144527037831E-4 0.0 14-15 0.0 2.928612722010809E-5 0.0 1.1714450888043236E-4 0.0 16-17 0.0 5.857225444021618E-5 0.0 1.244660406854594E-4 0.0 18-19 0.0 5.857225444021618E-5 0.0 1.903598269307026E-4 0.0 20-21 0.0 6.58937862452432E-5 0.0 3.29468931226216E-4 0.0 22-23 0.0 7.321531805027023E-5 0.0 6.955455214775672E-4 0.0 24-25 0.0 1.0250144527037831E-4 0.0 0.0015228786154456208 0.0 26-27 0.0 1.1714450888043236E-4 0.0 0.0032727247168470785 0.0 28-29 0.0 1.1714450888043236E-4 0.0 0.010140321549962427 0.0 30-31 0.0 1.317875724904864E-4 0.0 0.03193652173352787 0.0 32-33 0.0 1.317875724904864E-4 0.0 0.08288706156471093 0.0 34-35 0.0 1.610736997105945E-4 0.0 0.17127259351499713 0.0 36-37 7.321531805027022E-6 1.8303829512567556E-4 0.0 0.31492836906143234 0.0 38-39 1.4643063610054044E-5 1.9035982693070257E-4 0.0 0.5136420637816708 0.0 40-41 1.4643063610054044E-5 1.9035982693070257E-4 0.0 0.7345180352757259 0.0 42-43 1.4643063610054044E-5 1.9035982693070257E-4 0.0 0.9649339627117314 0.0 44-45 1.4643063610054044E-5 1.9035982693070257E-4 0.0 1.2437891445697957 0.0 46-47 1.4643063610054044E-5 1.9035982693070257E-4 0.0 1.5798474544205359 0.0 48-49 1.4643063610054044E-5 1.9035982693070257E-4 0.0 1.9428856089728008 0.0 50-51 1.4643063610054044E-5 1.9035982693070257E-4 0.0 2.3104484701805728 0.0 52-53 1.4643063610054044E-5 1.9035982693070257E-4 0.0 2.6730473328245354 0.0 54-55 1.4643063610054044E-5 1.9035982693070257E-4 0.0 3.0667773487034737 0.0 56-57 1.4643063610054044E-5 1.9035982693070257E-4 0.0 3.488146147146389 0.0 58-59 1.4643063610054044E-5 1.9035982693070257E-4 0.0 3.9347888733802576 0.0 60-61 1.4643063610054044E-5 1.9035982693070257E-4 0.0 4.38669578098194 0.0 62-63 1.4643063610054044E-5 1.9035982693070257E-4 0.0 4.830578289725313 0.0 64-65 1.4643063610054044E-5 1.9035982693070257E-4 0.0 5.314099571661103 0.0 66-67 1.4643063610054044E-5 1.9035982693070257E-4 0.0 5.836856942540033 0.0 68-69 1.4643063610054044E-5 1.9035982693070257E-4 0.0 6.341200660929319 0.0 70-71 1.4643063610054044E-5 1.9035982693070257E-4 0.0 6.922449749398609 0.0 72-73 1.4643063610054044E-5 1.9035982693070257E-4 0.0 7.54439191164024 0.0 74-75 1.4643063610054044E-5 1.9035982693070257E-4 0.0 8.090548898168034 0.0 76-77 1.4643063610054044E-5 2.1232442234578363E-4 0.0 8.639158597850516 0.0 78-79 1.4643063610054044E-5 2.342890177608647E-4 0.0 9.25720570517187 0.0 80-81 1.4643063610054044E-5 2.4893208137091873E-4 0.0 9.975997090130399 0.0 82-83 1.4643063610054044E-5 2.4893208137091873E-4 0.0 10.691874505430526 0.0 84-85 1.4643063610054044E-5 2.4893208137091873E-4 0.0 11.385926434419869 0.0 86-87 1.4643063610054044E-5 2.4893208137091873E-4 0.0 12.179778163443533 0.0 88 1.4643063610054044E-5 2.635751449809728E-4 0.0 12.819328609676253 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6055 0.0 40.58065 1 GTATCAA 9395 0.0 34.163525 1 TCAACGC 9620 0.0 31.974058 4 ATCAACG 9675 0.0 31.686497 3 CAACGCA 10540 0.0 29.231712 5 TATCAAC 11270 0.0 27.656153 2 AACGCAG 11665 0.0 26.456194 6 GTACATG 15730 0.0 23.431263 1 ACGCAGA 13645 0.0 22.279062 7 TACATGG 15770 0.0 22.036186 2 CGCAGAG 13910 0.0 21.823072 8 TCTGTCG 1995 0.0 21.542755 8 GTGGTAT 3860 0.0 20.953749 1 ACATGGG 15990 0.0 20.836794 3 TACCTGG 4570 0.0 20.718288 2 CGCCGTA 1625 0.0 18.655443 94 CTGTCGC 2300 0.0 18.489302 9 TCGCCGT 2110 0.0 17.798351 94 GCAGAGT 16255 0.0 17.784203 9 TGGTATC 4205 0.0 17.526487 2 >>END_MODULE