FastQCFastQC Report
Wed 25 May 2016
SRR2049495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6829172
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211690.3099790135612341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203160.297488480301858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123170.18035861448503565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123500.054.933021
GTATCAA217450.039.6862031
GTGGTAT52900.034.7982641
ATCAACG275750.030.9858193
TCAACGC278550.030.6743474
TGGTATC58400.030.3732412
TATCAAC284800.030.0011922
CAACGCA286450.029.9104715
AACGCAG303300.028.4655116
ACGCAGA344300.025.0342757
CGCAGAG344400.024.863258
GTACATG166200.023.8516041
TATAACG7500.023.8388332
GCAGAGT369650.022.9614729
TACATGG172100.022.4726142
ACATGGG176900.020.9851383
AGAGTAC344750.020.68698110-11
GAGTACT278950.020.44495212-13
CAGAGTA360550.020.40611310-11
ATAACGC7450.020.2095383
Produced by FastQC (version 0.11.5)