##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049495_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6829172
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754116750903332	31.0	31.0	34.0	30.0	34.0
2	31.927975748743773	33.0	31.0	34.0	30.0	34.0
3	31.951089824652243	33.0	31.0	34.0	30.0	34.0
4	35.575968799731505	37.0	35.0	37.0	33.0	37.0
5	35.70756118018407	37.0	35.0	37.0	33.0	37.0
6	35.778945236699265	37.0	35.0	37.0	33.0	37.0
7	35.75800638789007	37.0	35.0	37.0	33.0	37.0
8	35.7548629614249	37.0	35.0	37.0	33.0	37.0
9	37.498169617048745	39.0	37.0	39.0	35.0	39.0
10-11	37.454968991848496	39.0	37.0	39.0	34.5	39.0
12-13	37.436596120291014	39.0	37.0	39.0	34.0	39.0
14-15	38.69438425331797	40.0	38.0	41.0	34.0	41.0
16-17	38.701434083077714	40.0	38.0	41.0	34.0	41.0
18-19	38.774471634335754	40.0	38.0	41.0	34.5	41.0
20-21	38.798272689573494	40.0	38.0	41.0	34.5	41.0
22-23	38.76614595444367	40.0	38.0	41.0	34.0	41.0
24-25	38.7216626700865	40.0	38.0	41.0	34.0	41.0
26-27	38.623881562801465	40.0	38.0	41.0	34.0	41.0
28-29	38.530907260792375	40.0	38.0	41.0	34.0	41.0
30-31	38.40967645858092	40.0	38.0	41.0	34.0	41.0
32-33	38.25374298670468	40.0	38.0	41.0	34.0	41.0
34-35	38.14435586041763	40.0	38.0	41.0	34.0	41.0
36-37	37.99745811058793	40.0	37.5	41.0	33.0	41.0
38-39	37.898996466921616	40.0	37.0	41.0	33.0	41.0
40-41	37.77405745528155	40.0	37.0	41.0	33.0	41.0
42-43	37.631439067576565	40.0	37.0	41.0	33.0	41.0
44-45	37.44766371384408	40.0	37.0	41.0	33.0	41.0
46-47	37.200400722078754	40.0	37.0	41.0	32.0	41.0
48-49	36.97146952807749	40.0	37.0	41.0	31.0	41.0
50-51	36.76188291933488	40.0	36.0	41.0	31.0	41.0
52-53	36.65550508905032	40.0	36.0	41.0	31.0	41.0
54-55	36.52051749465382	39.5	36.0	41.0	30.5	41.0
56-57	36.349008854953425	39.0	35.0	41.0	30.0	41.0
58-59	36.17603195526486	39.0	35.0	41.0	30.0	41.0
60-61	35.984932067899294	39.0	35.0	41.0	30.0	41.0
62-63	35.77761242504948	39.0	35.0	41.0	29.0	41.0
64-65	35.49264427664144	38.0	35.0	40.0	29.0	41.0
66-67	35.235662244266216	37.5	35.0	40.0	29.0	41.0
68-69	34.91848704352446	37.0	34.0	40.0	28.5	41.0
70-71	34.6169436060477	36.5	34.0	39.0	28.0	41.0
72-73	34.29261087581335	36.0	34.0	39.0	28.0	41.0
74-75	33.96019114762375	35.5	34.0	39.0	28.0	40.5
76-77	33.6668099734492	35.0	34.0	37.5	28.0	40.0
78-79	33.33913708719007	35.0	34.0	37.0	27.0	39.0
80-81	33.03168663785303	35.0	33.5	37.0	27.0	39.0
82-83	32.73503208002376	35.0	33.0	36.0	26.0	39.0
84-85	32.46324869252085	35.0	33.0	36.0	26.0	37.0
86-87	32.183582724230696	35.0	33.0	35.5	26.0	37.0
88-89	31.973995017258314	35.0	33.0	35.0	26.0	36.5
90-91	31.823642529431094	35.0	33.0	35.0	25.5	36.0
92-93	31.66011963090108	35.0	33.0	35.0	25.0	36.0
94-95	31.473464791924997	35.0	33.0	35.0	25.0	36.0
96-97	31.29670961574844	35.0	33.0	35.0	24.5	35.5
98-99	31.104022420287556	35.0	33.0	35.0	24.0	35.0
100	30.98607927871783	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3669658876697284
1101	2	0.3798003247121926
1101	3	0.442012676687753
1101	4	0.33607970812028043
1101	5	0.2763695007062168
1101	6	0.2754667123291554
1101	7	0.30266521901212684
1101	8	0.3299044354163456
1101	9	0.34785395585110734
1101	10-11	0.3441668246839029
1101	12-13	0.26783626096188584
1101	14-15	0.44850052993420064
1101	16-17	0.43461821231882425
1101	18-19	0.4366985506735688
1101	20-21	0.3954332889862897
1101	22-23	0.45047978949811807
1101	24-25	0.44413751861350903
1101	26-27	0.4103938410971324
1101	28-29	0.4160431754348508
1101	30-31	0.4151664368106722
1101	32-33	0.44046414170028925
1101	34-35	0.48634044386331965
1101	36-37	0.4926549238702478
1101	38-39	0.5054825458737824
1101	40-41	0.49485671101360396
1101	42-43	0.43219331636939273
1101	44-45	0.4566153796268537
1101	46-47	0.46583238214108036
1101	48-49	0.48005472440893016
1101	50-51	0.5690305852097453
1101	52-53	0.5458615314489919
1101	54-55	0.4021978921067344
1101	56-57	0.39943885517151756
1101	58-59	0.4156929788197701
1101	60-61	0.47250463562015454
1101	62-63	0.43737954960254655
1101	64-65	0.4753368206029549
1101	66-67	0.4369542243042872
1101	68-69	0.39308949031431695
1101	70-71	0.37227294545899525
1101	72-73	0.35885874770268344
1101	74-75	0.38142359297657435
1101	76-77	0.3538205169714743
1101	78-79	0.36330007643118023
1101	80-81	0.3545887768174012
1101	82-83	0.3524288474421837
1101	84-85	0.3799241639591102
1101	86-87	0.4103699800123337
1101	88-89	0.4117710042263951
1101	90-91	0.4468157468840026
1101	92-93	0.42345180763149415
1101	94-95	0.353039273183434
1101	96-97	0.42206877826948386
1101	98-99	0.5413499006161793
1101	100	0.6221653281500217
1102	1	0.2655881018042976
1102	2	0.2848619269537167
1102	3	0.39575612394032333
1102	4	0.3419606983287764
1102	5	0.277992925655532
1102	6	0.1900435687367974
1102	7	0.282410457990558
1102	8	0.28417329228124544
1102	9	0.3003341897086642
1102	10-11	0.2822825029851259
1102	12-13	0.2883327300021179
1102	14-15	0.3683292894673258
1102	16-17	0.40119398622542235
1102	18-19	0.39093187630808046
1102	20-21	0.35942753120056636
1102	22-23	0.3373954663995491
1102	24-25	0.35014719579956477
1102	26-27	0.35684428751216046
1102	28-29	0.3405530520903852
1102	30-31	0.34818964759275417
1102	32-33	0.31221247420540976
1102	34-35	0.3636440364136604
1102	36-37	0.42351545387582235
1102	38-39	0.4299993631340797
1102	40-41	0.4105698552358703
1102	42-43	0.4068301885946042
1102	44-45	0.43924597381260355
1102	46-47	0.48491976843764206
1102	48-49	0.4951065398145076
1102	50-51	0.4970038586322403
1102	52-53	0.46523088127180046
1102	54-55	0.45713947437798197
1102	56-57	0.4850678722652404
1102	58-59	0.48168409717865757
1102	60-61	0.5120395801069861
1102	62-63	0.5351414553643039
1102	64-65	0.49021052492619077
1102	66-67	0.517177338501142
1102	68-69	0.464656947160087
1102	70-71	0.4683742386278098
1102	72-73	0.4845448527286109
1102	74-75	0.41545033355193084
1102	76-77	0.4267638699718006
1102	78-79	0.41499253415181414
1102	80-81	0.40724822992107335
1102	82-83	0.40314501286887605
1102	84-85	0.4167083426464373
1102	86-87	0.4512853349630248
1102	88-89	0.4408304181360734
1102	90-91	0.41607544681658126
1102	92-93	0.429842786478396
1102	94-95	0.40090243012042137
1102	96-97	0.4728041324269654
1102	98-99	0.4920149621950891
1102	100	0.5366423798277786
1103	1	0.2924024381224939
1103	2	0.28877474566842665
1103	3	0.39195878873695733
1103	4	0.336738182415516
1103	5	0.23523422281092365
1103	6	0.24074689883353528
1103	7	0.26198943091105065
1103	8	0.25335497315296607
1103	9	0.2983382444430802
1103	10-11	0.30334545475985664
1103	12-13	0.2510131701790499
1103	14-15	0.3421024401128463
1103	16-17	0.3470003704092619
1103	18-19	0.3728238909087267
1103	20-21	0.34263185025691456
1103	22-23	0.34380628471868135
1103	24-25	0.36410384340628354
1103	26-27	0.4003581059751298
1103	28-29	0.4134222037428543
1103	30-31	0.4177494257061838
1103	32-33	0.37489601611931533
1103	34-35	0.3945433378485532
1103	36-37	0.4378865205137217
1103	38-39	0.4176902098633164
1103	40-41	0.4308785679700762
1103	42-43	0.38248368861258797
1103	44-45	0.4267197214301177
1103	46-47	0.378760438053213
1103	48-49	0.35522125912978453
1103	50-51	0.3803669057067154
1103	52-53	0.4068682837244282
1103	54-55	0.37873662167902467
1103	56-57	0.40544394663499617
1103	58-59	0.3679169701962408
1103	60-61	0.3601283257465724
1103	62-63	0.35943171950642494
1103	64-65	0.36299959332622933
1103	66-67	0.44350328610426715
1103	68-69	0.40328872153344264
1103	70-71	0.42216831475971617
1103	72-73	0.4310127160364132
1103	74-75	0.4311587882575836
1103	76-77	0.37338090884690445
1103	78-79	0.33970000439312287
1103	80-81	0.2751197640671492
1103	82-83	0.31103084197664543
1103	84-85	0.34656793285753196
1103	86-87	0.40587644511514753
1103	88-89	0.39025032589310626
1103	90-91	0.3124671602340605
1103	92-93	0.37005307525460296
1103	94-95	0.38333790050557326
1103	96-97	0.37307362392191123
1103	98-99	0.38719373912176636
1103	100	0.36137581025896637
1104	1	0.29839836109415074
1104	2	0.28803679389178427
1104	3	0.36738401193651526
1104	4	0.31766372564415235
1104	5	0.21532215422823242
1104	6	0.23569888854792254
1104	7	0.23328985833857274
1104	8	0.22150391377378043
1104	9	0.2393096862900208
1104	10-11	0.2734335879935017
1104	12-13	0.2744011865707705
1104	14-15	0.41739025473901137
1104	16-17	0.3783628766668343
1104	18-19	0.3793993271450873
1104	20-21	0.33933041857102353
1104	22-23	0.37531566841644803
1104	24-25	0.3934589171111256
1104	26-27	0.40593505909180294
1104	28-29	0.3927907995649065
1104	30-31	0.42169018571686223
1104	32-33	0.4007433464235106
1104	34-35	0.3374962976775393
1104	36-37	0.37676108222957794
1104	38-39	0.3956779980291145
1104	40-41	0.40700478030657905
1104	42-43	0.42094049369845976
1104	44-45	0.42956602979057124
1104	46-47	0.47653772099784675
1104	48-49	0.46159170793884385
1104	50-51	0.5209837135291693
1104	52-53	0.5252170500131612
1104	54-55	0.5242537743323794
1104	56-57	0.46870390993066025
1104	58-59	0.4752969449053168
1104	60-61	0.46264587022824344
1104	62-63	0.47339611593259434
1104	64-65	0.5318813757934464
1104	66-67	0.5134933321590083
1104	68-69	0.4398783289148014
1104	70-71	0.4900155008308218
1104	72-73	0.5064028826393923
1104	74-75	0.5185622252785294
1104	76-77	0.46800112013040973
1104	78-79	0.4409139560617916
1104	80-81	0.44264323695640684
1104	82-83	0.4515656539819872
1104	84-85	0.4451189667071631
1104	86-87	0.4903004046144588
1104	88-89	0.4484254114598514
1104	90-91	0.48182057731031236
1104	92-93	0.5070769918013127
1104	94-95	0.48532313326199983
1104	96-97	0.4752086058760625
1104	98-99	0.6031656336803906
1104	100	0.6022997237255403
1105	1	0.2404751457279275
1105	2	0.25159979774849006
1105	3	0.34305357013684556
1105	4	0.24425399655652313
1105	5	0.1854170530606325
1105	6	0.24443308062176783
1105	7	0.17395347034478448
1105	8	0.17268703823337006
1105	9	0.19306448205237814
1105	10-11	0.22970797567739254
1105	12-13	0.23072710093622817
1105	14-15	0.2711203759789029
1105	16-17	0.26113767634812746
1105	18-19	0.29510393213109154
1105	20-21	0.2415041421018742
1105	22-23	0.2637093635363357
1105	24-25	0.24404980982065894
1105	26-27	0.25143978848748816
1105	28-29	0.3180788447688201
1105	30-31	0.29537043817318676
1105	32-33	0.29576691509445396
1105	34-35	0.2564200364905318
1105	36-37	0.2997471283419557
1105	38-39	0.26169146213440087
1105	40-41	0.261594339462377
1105	42-43	0.2819161908815886
1105	44-45	0.3852268002673611
1105	46-47	0.3702504368211592
1105	48-49	0.3505559297908363
1105	50-51	0.38785737928079556
1105	52-53	0.31737962518997875
1105	54-55	0.3299469445654566
1105	56-57	0.30402474620222364
1105	58-59	0.302838081310675
1105	60-61	0.3158791898455817
1105	62-63	0.33538229379851714
1105	64-65	0.33526015641359663
1105	66-67	0.35032350074918384
1105	68-69	0.3281941235304373
1105	70-71	0.2651650560819476
1105	72-73	0.28953364200219056
1105	74-75	0.30855041971437913
1105	76-77	0.2628636582492234
1105	78-79	0.210112825246334
1105	80-81	0.22810107792604128
1105	82-83	0.2410443949976937
1105	84-85	0.23891461360648947
1105	86-87	0.35568921259314124
1105	88-89	0.3227674715087403
1105	90-91	0.24683956992956624
1105	92-93	0.274471150897476
1105	94-95	0.3481594536405268
1105	96-97	0.3410616011730596
1105	98-99	0.33971064542480534
1105	100	0.3336830683585674
1106	1	0.208038346468431
1106	2	0.21460707936615364
1106	3	0.3041784711492781
1106	4	0.2701035487572625
1106	5	0.17809705805094467
1106	6	0.178488841784052
1106	7	0.205758465949323
1106	8	0.2145077797399253
1106	9	0.23093746826020833
1106	10-11	0.20844020559679421
1106	12-13	0.24146978369245176
1106	14-15	0.28502055310402596
1106	16-17	0.2862731822021658
1106	18-19	0.2389076657872593
1106	20-21	0.21520103018482928
1106	22-23	0.21355873074647036
1106	24-25	0.24607135775311662
1106	26-27	0.2708231476958787
1106	28-29	0.28647267065457527
1106	30-31	0.26457211742099673
1106	32-33	0.28499318516750805
1106	34-35	0.29296989143253427
1106	36-37	0.32429845742175445
1106	38-39	0.33450846819081903
1106	40-41	0.322771545167825
1106	42-43	0.3335086315176241
1106	44-45	0.28277749292356447
1106	46-47	0.24238288603832103
1106	48-49	0.2652511710427419
1106	50-51	0.26680609063754446
1106	52-53	0.2509407728049098
1106	54-55	0.1453776054260416
1106	56-57	0.22863524237391886
1106	58-59	0.24115009225926798
1106	60-61	0.23433722955576997
1106	62-63	0.20997697944687843
1106	64-65	0.1724139121012982
1106	66-67	0.23378815281229492
1106	68-69	0.19934436719382376
1106	70-71	0.21453732019470095
1106	72-73	0.19757881848085646
1106	74-75	0.2017119596675485
1106	76-77	0.19286198629301765
1106	78-79	0.21436716532782185
1106	80-81	0.12648118046079304
1106	82-83	0.12454790183783615
1106	84-85	0.2169999551318753
1106	86-87	0.3005005036167745
1106	88-89	0.28183638720969384
1106	90-91	0.2398505855988482
1106	92-93	0.24376143369726933
1106	94-95	0.24845933998897607
1106	96-97	0.278924913472153
1106	98-99	0.30847498297979214
1106	100	0.38169765773176323
1107	1	0.20412058703828606
1107	2	0.2095124723149766
1107	3	0.2693382310221395
1107	4	0.23936939011080227
1107	5	0.22716519429038584
1107	6	0.2002727471869079
1107	7	0.17995900580586977
1107	8	0.24009116510961093
1107	9	0.2085905790431326
1107	10-11	0.1852732894859983
1107	12-13	0.16495453456219877
1107	14-15	0.242831788331479
1107	16-17	0.2194264532240311
1107	18-19	0.2310470373202378
1107	20-21	0.2548365444196605
1107	22-23	0.22301448002044566
1107	24-25	0.2697589678141412
1107	26-27	0.2831136394146654
1107	28-29	0.28370631884915554
1107	30-31	0.23399489381665006
1107	32-33	0.2901181880584147
1107	34-35	0.25754915684438373
1107	36-37	0.302448143716866
1107	38-39	0.2570258384220594
1107	40-41	0.2252217141620818
1107	42-43	0.19269924316782294
1107	44-45	0.18797187762255874
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2216	96-97	-1.6289687035526974
2216	98-99	-1.6662548815543268
2216	100	-1.6567602081073396
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	441.0
3	40.0
4	59.0
5	129.0
6	469.0
7	1206.0
8	2667.0
9	4483.0
10	6359.0
11	9260.0
12	13553.0
13	18832.0
14	21134.0
15	25933.0
16	32937.0
17	44796.0
18	45893.0
19	26017.0
20	19637.0
21	18675.0
22	19667.0
23	21944.0
24	25195.0
25	31317.0
26	39217.0
27	50917.0
28	65681.0
29	86284.0
30	111917.0
31	144541.0
32	183710.0
33	240061.0
34	325367.0
35	469534.0
36	755507.0
37	1378893.0
38	1866441.0
39	717914.0
40	2545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87835737281745	20.586743825100648	14.217422155982975	23.317476646098932
2	16.097089039140133	20.89643900254702	39.089029182824106	23.917442775488734
3	17.986106952073687	25.4115030945923	30.896971242922454	25.705418710411553
4	12.809815278163223	17.76265220921898	37.79548845417051	31.632044058447285
5	12.442750047773883	37.11499924220736	35.31533325669988	15.126917453318875
6	29.98727934153057	38.1693849319283	17.57234426052588	14.270991466015253
7	26.017341487372114	32.818854174415286	22.70442156091544	18.459382777297158
8	24.775917197575342	35.40320847095373	20.886353426154738	18.934520905316194
9	24.340593559512048	16.611911370807473	22.368055746728885	36.679439322951595
10-11	23.454951786248756	26.897301751954707	28.855694365290553	20.79205209650599
12-13	24.47386187328873	24.45604126357055	29.17266025607938	21.89743660706134
14-15	21.77369603827347	26.602116597202386	27.0887790300694	24.535408334454747
16-17	20.51764840598538	29.688079316204075	27.743172964453084	22.05109931335746
18-19	20.27848324804237	27.782284587355537	30.998948335171526	20.940283829430566
20-21	21.51472389332118	27.24743936746651	30.675548953811678	20.56228778540063
22-23	21.119763860099	27.259622396390075	30.74486189540987	20.87575184810106
24-25	20.91208861044941	27.200025127497156	30.872410300985244	21.015475961068194
26-27	20.72303353342349	27.07299121130579	30.956280714285768	21.24769454098495
28-29	20.81877566201407	27.06561275471654	31.05528351949581	21.060328063773586
30-31	21.232328699082988	27.592064139149343	30.463540714864745	20.712066446902924
32-33	20.707632061609825	27.32979063855559	30.96637432027799	20.996202979556593
34-35	20.806058186849004	26.87994972157679	30.95238339289155	21.36160869868265
36-37	21.404622698037183	27.32203113349613	30.254824450167604	21.018521718299084
38-39	21.23212740871075	27.34999206345952	30.456788905009276	20.96109162282045
40-41	20.941755457323378	27.101462666337884	30.618982799086037	21.337799077252704
42-43	20.939046490555516	27.12664873574717	30.43830203720158	21.49600273649573
44-45	21.19552707121742	27.34714398758737	30.18267075422906	21.27465818696615
46-47	21.18698284360095	27.707722107453144	29.68181208497897	21.423482963966936
48-49	21.119046349982106	27.383385570022252	29.765394692065154	21.732173387930484
50-51	21.17530500037193	27.309152559051082	29.572750547211285	21.942791893365694
52-53	21.086824288508183	27.44933060699013	29.48556574647703	21.978279358024665
54-55	21.201208579898122	27.880078287675286	29.23702170629177	21.681691426134822
56-57	20.881455321377175	27.570113917177665	29.253626940425576	22.294803821019592
58-59	20.71301014422053	27.14416027331035	29.646766926442414	22.496062656026705
60-61	21.094709578262197	27.47383577394156	29.170710592733645	22.260744055062606
62-63	20.83670611898485	27.426794932094257	29.29013209800544	22.446366850915457
64-65	20.723639703319815	27.15894401253915	29.53095192213639	22.586464362004648
66-67	20.704208357909277	27.23181521859458	29.18071180517931	22.883264618316833
68-69	21.126165972414515	27.384846564831953	28.930590550448947	22.55839691230458
70-71	21.02068157018157	27.089286958946126	28.976089634292414	22.913941836579895
72-73	20.702370653426215	27.146197225666597	29.214420137609654	22.937011983297538
74-75	20.746753779228285	27.102985544953327	29.01432999491007	23.135930680908317
76-77	20.79455606038331	27.19682561809836	28.71186287298079	23.29675544853754
78-79	20.8663961991237	27.291079051866102	28.7355242121947	23.107000536815498
80-81	20.65381441774275	27.03507974266216	28.789989391074005	23.521116448521084
82-83	20.470607788160965	26.950106144288142	28.933882303757535	23.645403763793357
84-85	20.53992782726808	26.8703585149122	28.712895208965307	23.876818448854415
86-87	20.758923684959445	26.951270736135413	28.61326589909186	23.67653967981328
88-89	20.507888990015548	26.804957762433627	28.65262020513622	24.0345330424146
90-91	20.31043303944696	26.608562973245654	28.63044118099271	24.450562806314675
92-93	20.43469767849144	26.68569981829329	28.441866080589683	24.437736422625584
94-95	20.33691675446451	26.410914413025953	28.681209260030737	24.5709595724788
96-97	20.279698622322005	26.414585838517468	28.678659726244998	24.627055812915533
98-99	20.32316804461566	26.36172630896735	28.576006621008126	24.739099025408866
100	20.349887774443033	26.307096802157353	28.465198362772043	24.87781706062757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1451.0
1	1071.5
2	2282.5
3	3562.0
4	2121.5
5	1069.5
6	2012.5
7	4630.5
8	7343.5
9	8313.5
10	6586.5
11	4416.5
12	4098.5
13	4550.5
14	5180.5
15	5912.5
16	6674.5
17	7427.0
18	8386.0
19	9658.5
20	11348.0
21	13428.5
22	16827.0
23	22348.0
24	28946.5
25	36950.0
26	48368.0
27	63129.5
28	81776.0
29	100537.0
30	115867.5
31	131503.5
32	147891.0
33	162810.5
34	177700.0
35	191853.0
36	203082.5
37	213403.0
38	223226.5
39	229492.0
40	233837.5
41	236742.0
42	236127.5
43	235877.5
44	234425.0
45	231222.5
46	229315.5
47	226205.5
48	221940.0
49	220211.5
50	216527.0
51	207159.5
52	195859.5
53	187381.0
54	182031.0
55	175619.5
56	164578.0
57	149333.0
58	133566.0
59	118379.5
60	101411.5
61	83261.0
62	67956.5
63	54320.0
64	42164.0
65	32740.5
66	25042.0
67	18748.5
68	14150.5
69	10586.0
70	7679.5
71	5560.0
72	4059.5
73	2869.0
74	1978.5
75	1361.5
76	914.0
77	581.0
78	364.0
79	248.5
80	142.0
81	68.5
82	43.0
83	24.5
84	14.0
85	6.5
86	6.5
87	4.0
88	2.5
89	2.5
90	2.0
91	1.0
92	1.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	1.0
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059758342592630555
2	2.635751449809728E-4
3	1.4643063610054046E-5
4	0.11789130512454513
5	0.0018596690784768637
6	0.0018010968240366476
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.321531805027023E-6
14-15	4.392919083016214E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.321531805027023E-6
28-29	1.7571676332064855E-4
30-31	2.928612722010809E-4
32-33	2.196459541508107E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8303829512567556E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.4643063610054046E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.4643063610054045E-4
80-81	2.489320813709188E-4
82-83	3.587550584463241E-4
84-85	0.0
86-87	7.321531805027023E-6
88-89	3.660765902513511E-5
90-91	2.9286127220108092E-5
92-93	5.125072263518916E-4
94-95	5.417933535719996E-4
96-97	0.0
98-99	7.321531805027023E-6
100	9.957283254836751E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6829172.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.72344765987835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6734270646963	47.94271709991161
2	13.762973696352827	16.989963731822325
3	3.9158932144499294	7.251072895913191
4	1.6170073792323707	3.992290813507452
5	0.8227804246723974	2.5392422238919607
6	0.5001162048258025	1.8521337835453455
7	0.3306605446656621	1.4286656175300503
8	0.2347406602852834	1.159120228701118
9	0.17717341685277463	0.9842178709650641
>10	0.8929315970474189	9.757627718869792
>50	0.049352969052771416	2.056117047071657
>100	0.021683832680779332	2.345021726113442
>500	7.839193225648954E-4	0.34089930265509316
>1k	3.563068988207607E-4	0.406509186556001
>5k	4.7507585797076446E-5	0.16557412555521733
>10k+	7.126137869561467E-5	0.7888266273905785
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21169	0.3099790135612341	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20316	0.297488480301858	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12317	0.18035861448503565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4643063610054044E-5	0.0
5	0.0	0.0	0.0	2.928612722010809E-5	0.0
6	0.0	0.0	0.0	2.928612722010809E-5	0.0
7	0.0	0.0	0.0	4.392919083016214E-5	0.0
8	0.0	0.0	0.0	4.392919083016214E-5	0.0
9	0.0	0.0	0.0	5.857225444021618E-5	0.0
10-11	0.0	0.0	0.0	7.321531805027023E-5	0.0
12-13	0.0	0.0	0.0	1.1714450888043236E-4	0.0
14-15	0.0	1.4643063610054044E-5	0.0	1.317875724904864E-4	0.0
16-17	0.0	2.928612722010809E-5	0.0	1.683952315156215E-4	0.0
18-19	0.0	3.660765902513511E-5	0.0	2.2696748595583767E-4	0.0
20-21	0.0	4.392919083016214E-5	0.0	3.660765902513511E-4	0.0
22-23	0.0	5.1250722635189154E-5	0.0	7.321531805027022E-4	0.0
24-25	0.0	5.857225444021618E-5	0.0	0.0015594862744707557	0.0
26-27	0.0	5.857225444021618E-5	0.0	0.003323975439482268	0.0
28-29	0.0	6.58937862452432E-5	0.0	0.010257466058842857	0.0
30-31	0.0	7.321531805027023E-5	0.0	0.03206830930601836	0.0
32-33	0.0	7.321531805027023E-5	0.0	0.08354599942716334	0.0
34-35	0.0	8.785838166032427E-5	0.0	0.17260511230351205	0.0
36-37	0.0	1.0250144527037831E-4	0.0	0.31738840374792143	0.0
38-39	0.0	1.244660406854594E-4	0.0	0.5176981924016557	0.0
40-41	0.0	1.317875724904864E-4	0.0	0.7400677563839364	0.0
42-43	0.0	1.4643063610054045E-4	0.0	0.9719553117127522	0.0
44-45	0.0	1.4643063610054045E-4	0.0	1.2515938974739544	0.0
46-47	0.0	1.610736997105945E-4	0.0	1.5888602600725241	0.0
48-49	0.0	1.7571676332064855E-4	0.0	1.9529087860138827	0.0
50-51	0.0	1.7571676332064855E-4	0.0	2.321379517165478	0.0
52-53	0.0	1.7571676332064855E-4	0.0	2.685376792384201	0.0
54-55	0.0	1.7571676332064855E-4	0.0	3.0953752519339095	0.0
56-57	0.0	1.7571676332064855E-4	0.0	3.5770954370456627	0.0
58-59	0.0	1.7571676332064855E-4	0.0	4.08985891701073	0.0
60-61	0.0	1.7571676332064855E-4	0.0	4.608289262592889	0.0
62-63	0.0	1.9035982693070257E-4	0.0	5.119090572034208	0.0
64-65	0.0	1.9035982693070257E-4	0.0	5.672129798458729	0.0
66-67	0.0	2.0500289054075662E-4	0.0	6.2913629939325	0.0
68-69	0.0	2.1232442234578363E-4	0.0	6.9539103715648105	0.0
70-71	0.0	2.1964595415081066E-4	0.0	7.640113618459163	0.0
72-73	0.0	2.342890177608647E-4	0.0	8.351422397912954	0.0
74-75	0.0	2.342890177608647E-4	0.0	9.047465783553262	0.0
76-77	0.0	2.8553974039605384E-4	0.0	9.773944776907069	0.0
78-79	0.0	2.928612722010809E-4	0.0	10.561888029764077	0.0
80-81	0.0	3.07504335811135E-4	0.0	11.384052122277781	0.0
82-83	0.0	3.29468931226216E-4	0.0	12.204356545713008	0.0
84-85	0.0	3.4411199483627003E-4	0.0	13.000397412746377	0.0
86-87	0.0	3.514335266412971E-4	0.0	13.824662784888124	0.0
88	0.0	3.514335266412971E-4	0.0	14.469162586621042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12350	0.0	54.93302	1
GTATCAA	21745	0.0	39.686203	1
GTGGTAT	5290	0.0	34.798264	1
ATCAACG	27575	0.0	30.985819	3
TCAACGC	27855	0.0	30.674347	4
TGGTATC	5840	0.0	30.373241	2
TATCAAC	28480	0.0	30.001192	2
CAACGCA	28645	0.0	29.910471	5
AACGCAG	30330	0.0	28.465511	6
ACGCAGA	34430	0.0	25.034275	7
CGCAGAG	34440	0.0	24.86325	8
GTACATG	16620	0.0	23.851604	1
TATAACG	750	0.0	23.838833	2
GCAGAGT	36965	0.0	22.961472	9
TACATGG	17210	0.0	22.472614	2
ACATGGG	17690	0.0	20.985138	3
AGAGTAC	34475	0.0	20.686981	10-11
GAGTACT	27895	0.0	20.444952	12-13
CAGAGTA	36055	0.0	20.406113	10-11
ATAACGC	745	0.0	20.209538	3
>>END_MODULE