FastQCFastQC Report
Wed 25 May 2016
SRR2049494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6741311
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60950.043.0962371
GTATCAA96200.032.9998741
ATCAACG100500.030.360853
TCAACGC100200.030.3495624
CAACGCA108700.028.1176155
TATCAAC117200.026.7336042
AACGCAG120200.025.5116066
GTACATG160850.023.3015981
TACATGG163800.022.0973012
CGCAGAG144500.021.160928
ACGCAGA143050.021.0065547
ACATGGG168450.020.2716243
GTGGTAT38800.019.3990881
GCAGAGT167850.017.3009289
CATGGGG114150.016.773724
TGGTATC42300.016.5811212
TATACCG5351.8189894E-1216.2678015
AGAGTAC140050.016.14888610-11
GTATAGG21900.016.1324621
CAGAGTA157350.015.9400810-11
Produced by FastQC (version 0.11.5)