##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6741311 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.80187340414943 31.0 31.0 34.0 28.0 34.0 2 30.92318259756893 31.0 31.0 34.0 27.0 34.0 3 31.11431960341245 31.0 31.0 34.0 28.0 34.0 4 34.69799034638811 37.0 35.0 37.0 32.0 37.0 5 34.700468499376456 37.0 35.0 37.0 32.0 37.0 6 34.65029606259079 37.0 35.0 37.0 32.0 37.0 7 30.753829188417505 37.0 33.0 37.0 2.0 37.0 8 32.52530746615903 37.0 33.0 37.0 17.0 37.0 9 35.30828054068415 39.0 34.0 39.0 28.0 39.0 10-11 35.85281787177598 39.0 36.0 39.0 30.5 39.0 12-13 36.005733009499195 39.0 37.0 39.0 31.0 39.0 14-15 37.20340998954061 40.0 37.0 41.0 31.0 41.0 16-17 37.218804547068075 40.0 37.0 41.0 31.0 41.0 18-19 37.21315371742974 40.0 37.0 41.0 31.0 41.0 20-21 37.290175827817464 40.0 38.0 41.0 31.0 41.0 22-23 37.2522986997633 40.0 37.0 41.0 31.0 41.0 24-25 37.227591191090276 40.0 37.0 41.0 31.0 41.0 26-27 37.25621158851743 40.0 37.0 41.0 31.0 41.0 28-29 37.2412082011941 40.0 37.0 41.0 31.0 41.0 30-31 37.16371014480714 40.0 37.0 41.0 31.0 41.0 32-33 37.11702271264447 40.0 37.0 41.0 31.0 41.0 34-35 37.05992054067822 40.0 37.0 41.0 30.0 41.0 36-37 36.89086514477673 40.0 37.0 41.0 30.0 41.0 38-39 36.7775563981546 40.0 37.0 41.0 30.0 41.0 40-41 36.70286068985691 40.0 37.0 41.0 30.0 41.0 42-43 36.583722290812574 40.0 36.0 41.0 30.0 41.0 44-45 36.462211875405245 40.0 36.0 41.0 30.0 41.0 46-47 36.30804193724336 40.0 36.0 41.0 30.0 41.0 48-49 36.227307344224286 40.0 36.0 41.0 29.0 41.0 50-51 36.09207393042689 39.0 35.0 41.0 29.0 41.0 52-53 35.96801645851971 39.0 35.0 41.0 29.0 41.0 54-55 35.84127701570214 39.0 35.0 41.0 28.0 41.0 56-57 35.64336172889813 39.0 35.0 41.0 28.0 41.0 58-59 35.46210203030242 39.0 35.0 41.0 28.0 41.0 60-61 35.23472578256663 38.5 34.5 40.0 28.0 41.0 62-63 34.98708671058196 38.0 34.0 40.0 27.5 41.0 64-65 34.62370019718716 37.5 34.0 40.0 26.5 41.0 66-67 31.132783816085627 36.0 30.0 40.0 6.5 41.0 68-69 31.482550723442365 36.0 30.0 39.0 11.0 41.0 70-71 31.63518090175635 35.5 30.0 39.0 15.5 41.0 72-73 32.86695481042189 35.0 31.5 39.0 24.0 40.5 74-75 32.9519513340951 35.0 33.0 38.5 25.0 40.0 76-77 32.65247679568559 35.0 33.0 37.5 24.5 39.5 78-79 29.31017038080575 35.0 29.0 37.0 6.5 39.0 80-81 31.06426517038006 35.0 30.0 36.0 19.0 39.0 82-83 31.463114741331474 35.0 32.0 36.0 21.5 37.5 84-85 29.63090814234798 35.0 30.0 35.5 11.0 37.0 86-87 29.255812185493298 34.0 28.0 35.0 11.0 37.0 88-89 30.487593733622436 34.0 30.5 35.0 18.0 36.0 90-91 30.634796480981223 35.0 31.5 35.0 18.0 36.0 92-93 30.483120048904436 35.0 32.0 35.0 12.5 36.0 94-95 30.267142029198773 35.0 32.0 35.0 4.5 36.0 96-97 30.0682152180785 35.0 31.5 35.0 2.0 35.0 98-99 29.873917625221562 35.0 31.0 35.0 2.0 35.0 100 29.657270076992443 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3844915842689929 1101 2 0.38031360379666523 1101 3 0.3449500877706946 1101 4 0.4116217546846528 1101 5 0.36867481728140206 1101 6 0.3960891486216198 1101 7 -12.087659478832162 1101 8 -6.52212630987318 1101 9 -2.143635966932365 1101 10-11 -0.022038111908351254 1101 12-13 0.4087753482884864 1101 14-15 0.6106103834550041 1101 16-17 0.6563017867450895 1101 18-19 0.6491592114593558 1101 20-21 0.6268028196921378 1101 22-23 0.6047677565055523 1101 24-25 0.6384762225529386 1101 26-27 0.5743257050500432 1101 28-29 0.5245384273396851 1101 30-31 0.6035462633495712 1101 32-33 0.6135682144146131 1101 34-35 0.6117143166426828 1101 36-37 0.6884900370641063 1101 38-39 0.6637355412089221 1101 40-41 0.6452957078446317 1101 42-43 0.6414025039887861 1101 44-45 0.6081111371660057 1101 46-47 0.6210096293401719 1101 48-49 0.5653400775048283 1101 50-51 0.6335848238526651 1101 52-53 0.6190962921344436 1101 54-55 0.5724925148020574 1101 56-57 0.5903440171102048 1101 58-59 0.5290274602287184 1101 60-61 0.5137589410646228 1101 62-63 0.5896319307715672 1101 64-65 0.41311237566029746 1101 66-67 -10.22533432810108 1101 68-69 -8.131569194721386 1101 70-71 -6.631809143979659 1101 72-73 -1.4362146420233266 1101 74-75 -0.1340686199760981 1101 76-77 0.12745378112673933 1101 78-79 3.2586685927718904 1101 80-81 1.1596638881674757 1101 82-83 0.42089278302326605 1101 84-85 -5.348585425199406 1101 86-87 -5.8586253898663685 1101 88-89 -1.2531893470122313 1101 90-91 0.05917958871128093 1101 92-93 0.3666251287249942 1101 94-95 0.44737215276690634 1101 96-97 0.5576265020121625 1101 98-99 0.5693550527457489 1101 100 0.6059147445622095 1102 1 0.2022700328183049 1102 2 0.221876678671876 1102 3 0.2402176130054272 1102 4 0.22607695560387953 1102 5 0.21908030379282906 1102 6 0.21188579267753482 1102 7 -12.258726986868623 1102 8 -6.672567991723607 1102 9 -2.3208815038276995 1102 10-11 -0.2667053538981037 1102 12-13 0.24805751903484463 1102 14-15 0.4265649049986848 1102 16-17 0.4182265057047303 1102 18-19 0.3901491221731348 1102 20-21 0.42367300324418977 1102 22-23 0.4027020501195153 1102 24-25 0.4514236243637555 1102 26-27 0.41230909928660964 1102 28-29 0.41983644440546186 1102 30-31 0.38672353079013533 1102 32-33 0.4362405086155974 1102 34-35 0.43209695402373427 1102 36-37 0.5457376107698764 1102 38-39 0.48620736807323084 1102 40-41 0.46247324795675127 1102 42-43 0.4088979543443543 1102 44-45 0.38605588026464943 1102 46-47 0.3766291696023316 1102 48-49 0.32813781158886 1102 50-51 0.3607799559963638 1102 52-53 0.3518597700721813 1102 54-55 0.30894275674047833 1102 56-57 0.32490107281022773 1102 58-59 0.3329550548532083 1102 60-61 0.30647886284759096 1102 62-63 0.26797100015211583 1102 64-65 0.1525295769577113 1102 66-67 -10.409825436990257 1102 68-69 -6.851639400517115 1102 70-71 -6.382960945476523 1102 72-73 -1.2343950639425216 1102 74-75 -0.26163360815887415 1102 76-77 -0.10763311949489918 1102 78-79 3.026452657082892 1102 80-81 0.9246141864828807 1102 82-83 0.29308864784836786 1102 84-85 -5.605716230547895 1102 86-87 -6.117398937128048 1102 88-89 -1.4934350212304892 1102 90-91 -0.16747221848908822 1102 92-93 0.12885198553491506 1102 94-95 0.13729556781688146 1102 96-97 0.27175341669075337 1102 98-99 0.323577430985722 1102 100 0.38980389205887533 1103 1 0.21046588564337654 1103 2 0.23188209101953916 1103 3 0.2130387466903727 1103 4 0.2361966314431143 1103 5 0.26196929033125826 1103 6 0.2772535896275272 1103 7 -12.205974503027662 1103 8 -6.60771392596855 1103 9 -2.304874626201382 1103 10-11 -0.30160047290844716 1103 12-13 0.27349763008246697 1103 14-15 0.4979900199830567 1103 16-17 0.44075945650517667 1103 18-19 0.44349905820671864 1103 20-21 0.3900550192234036 1103 22-23 0.4276857519809738 1103 24-25 0.4654970389847719 1103 26-27 0.43312032335661854 1103 28-29 0.42031061147006454 1103 30-31 0.4305866162367309 1103 32-33 0.47855124895254164 1103 34-35 0.42780623835840714 1103 36-37 0.4492664722268813 1103 38-39 0.5295606967072075 1103 40-41 0.49456386945458775 1103 42-43 0.5235654429330197 1103 44-45 0.4493125614338709 1103 46-47 0.46990109950403536 1103 48-49 0.41770669532947124 1103 50-51 0.40612674389635117 1103 52-53 0.3857650406274118 1103 54-55 0.3907398574201224 1103 56-57 0.3638408287425605 1103 58-59 0.33217589094147826 1103 60-61 0.2896798358609445 1103 62-63 0.2363528703476021 1103 64-65 0.14185167481089422 1103 66-67 -10.404170778849291 1103 68-69 -8.37140844324147 1103 70-71 -6.897355418311495 1103 72-73 -1.7429583938643702 1103 74-75 -0.5577937755970623 1103 76-77 -0.16756775084176212 1103 78-79 2.993739452823398 1103 80-81 0.854164602664131 1103 82-83 0.1955513283984267 1103 84-85 -5.573399550200612 1103 86-87 -6.093441566648728 1103 88-89 -1.5062269618089097 1103 90-91 -0.18742976426403857 1103 92-93 0.21423478768077686 1103 94-95 0.2663010792704199 1103 96-97 0.29976112669648103 1103 98-99 0.2727826739655015 1103 100 0.4100826070345249 1104 1 0.17416755319263544 1104 2 0.15653145984633454 1104 3 0.18278362433642314 1104 4 0.18367774994428032 1104 5 0.16828723996952277 1104 6 0.20382624122620285 1104 7 -12.30566231666685 1104 8 -6.684319166898209 1104 9 -2.366728787797129 1104 10-11 -0.34277127244396866 1104 12-13 0.19046644430066806 1104 14-15 0.3875982096731079 1104 16-17 0.4251651525196678 1104 18-19 0.4306169284294654 1104 20-21 0.41447101664593333 1104 22-23 0.42513106637704823 1104 24-25 0.5205052507896326 1104 26-27 0.47117989276765115 1104 28-29 0.4650723843437916 1104 30-31 0.40272939742613545 1104 32-33 0.42317524579287635 1104 34-35 0.4425628993880295 1104 36-37 0.46268674105971996 1104 38-39 0.47780827388032776 1104 40-41 0.488168482620118 1104 42-43 0.46568442419582823 1104 44-45 0.3756203651037282 1104 46-47 0.3867464805271865 1104 48-49 0.3357132456966738 1104 50-51 0.4024886440892743 1104 52-53 0.37904414908222606 1104 54-55 0.3394447803697602 1104 56-57 0.3469645011026756 1104 58-59 0.375108810616986 1104 60-61 0.3213060417103506 1104 62-63 0.2722741910474653 1104 64-65 0.18331770365696087 1104 66-67 -10.465572116548593 1104 68-69 -8.408429923231623 1104 70-71 -6.85396857959671 1104 72-73 -1.7161219188253227 1104 74-75 -0.46866674220827775 1104 76-77 -0.17111177434706093 1104 78-79 2.994846324279422 1104 80-81 0.9617410480495359 1104 82-83 0.23866279168235138 1104 84-85 -5.550062445101165 1104 86-87 -6.015857691955677 1104 88-89 -1.4317910575159125 1104 90-91 -0.11390169804958106 1104 92-93 0.25145512441246964 1104 94-95 0.2479374416863891 1104 96-97 0.4027062837099358 1104 98-99 0.4725139047188094 1104 100 0.5128770229365642 1105 1 0.12152338991113609 1105 2 0.14550816372853603 1105 3 0.1449353172449328 1105 4 0.17152115843676796 1105 5 0.171382647365931 1105 6 0.15768754421062425 1105 7 -12.196887465298733 1105 8 -6.647951275432241 1105 9 -2.4399186750015787 1105 10-11 -0.3971085514943553 1105 12-13 0.16311679800682555 1105 14-15 0.3037225151948988 1105 16-17 0.31719159393303187 1105 18-19 0.32833679518171976 1105 20-21 0.3565485970920719 1105 22-23 0.3526921497608626 1105 24-25 0.4149116164227209 1105 26-27 0.3195417734648913 1105 28-29 0.3376973062990416 1105 30-31 0.37328104892209524 1105 32-33 0.37903342645077487 1105 34-35 0.3683309736212337 1105 36-37 0.4219325431017964 1105 38-39 0.3710950767815291 1105 40-41 0.37966958159849185 1105 42-43 0.36850502725944523 1105 44-45 0.30291071128423397 1105 46-47 0.3189644320467053 1105 48-49 0.3020568421598 1105 50-51 0.35329487935964465 1105 52-53 0.34082932730174775 1105 54-55 0.2831025040168882 1105 56-57 0.26453610001563277 1105 58-59 0.26926487710392877 1105 60-61 0.2217097408865314 1105 62-63 0.18681856306438505 1105 64-65 0.09378997687949919 1105 66-67 -10.326823028056193 1105 68-69 -8.397840951677487 1105 70-71 -6.83390760484037 1105 72-73 -1.7953573062849983 1105 74-75 -0.5117309673033148 1105 76-77 -0.2488485903981612 1105 78-79 2.91836065771664 1105 80-81 0.8611309786269494 1105 82-83 0.16219283769894943 1105 84-85 -5.508851680160429 1105 86-87 -6.0173956472224255 1105 88-89 -1.534170089307942 1105 90-91 -0.2206413168600676 1105 92-93 0.14217646591367483 1105 94-95 0.20626622794620175 1105 96-97 0.2191699848475075 1105 98-99 0.27747201840561075 1105 100 0.39496803271264724 1106 1 0.1070919523191236 1106 2 0.15064414803567772 1106 3 0.15464023591428955 1106 4 0.10600103254630255 1106 5 0.15581340589289994 1106 6 0.1417450007477541 1106 7 -12.244527323127677 1106 8 -6.730146948614493 1106 9 -2.5952831215779426 1106 10-11 -0.4327012676004358 1106 12-13 0.1185686041101448 1106 14-15 0.21515316858694433 1106 16-17 0.259329761945601 1106 18-19 0.26501282031664886 1106 20-21 0.2865498605017791 1106 22-23 0.27426769565305165 1106 24-25 0.32504626404143977 1106 26-27 0.25647636168781673 1106 28-29 0.24449025644459965 1106 30-31 0.20995999106787622 1106 32-33 0.26063898221131865 1106 34-35 0.28636075851689924 1106 36-37 0.33678377578393537 1106 38-39 0.32843650743539854 1106 40-41 0.3479313678185392 1106 42-43 0.31857218683968824 1106 44-45 0.26478561977420867 1106 46-47 0.31793492042643123 1106 48-49 0.2513233034670179 1106 50-51 0.2914658560629988 1106 52-53 0.2852956469713561 1106 54-55 0.24462416673381426 1106 56-57 0.24111271964114422 1106 58-59 0.21363091568962034 1106 60-61 0.19181132684978053 1106 62-63 0.07957651577138591 1106 64-65 0.07204368924705307 1106 66-67 -10.484449793265991 1106 68-69 -8.411838155211285 1106 70-71 -6.886764600197608 1106 72-73 -1.7590256533666917 1106 74-75 -0.5138771003106228 1106 76-77 -0.2766349000792445 1106 78-79 2.854452842422525 1106 80-81 0.7855924708556614 1106 82-83 0.07011067016855677 1106 84-85 -5.636462365995865 1106 86-87 -6.072939544159482 1106 88-89 -1.549706230483352 1106 90-91 -0.34002237051171846 1106 92-93 -0.019040272563767502 1106 94-95 0.06047295108600892 1106 96-97 0.06716554363957883 1106 98-99 0.10632622738130948 1106 100 0.17158522659250153 1107 1 0.06757965047212622 1107 2 0.11639485451514275 1107 3 0.12837734423421665 1107 4 0.16794798209672024 1107 5 0.14025616356370563 1107 6 0.16543438655301657 1107 7 -12.228573501370413 1107 8 -6.705498335001934 1107 9 -2.501647360622229 1107 10-11 -0.43495230500512605 1107 12-13 0.15681648011846505 1107 14-15 0.26290194584766624 1107 16-17 0.27408173231549426 1107 18-19 0.26556728194046286 1107 20-21 0.27460121090685163 1107 22-23 0.2790137394343901 1107 24-25 0.4042248876497112 1107 26-27 0.3571600404230608 1107 28-29 0.307146872759283 1107 30-31 0.2852751662783959 1107 32-33 0.30186912069195415 1107 34-35 0.2929780822496255 1107 36-37 0.3190489800736813 1107 38-39 0.37958239006258054 1107 40-41 0.356428835530167 1107 42-43 0.3939728905345561 1107 44-45 0.352429078476554 1107 46-47 0.393477384495192 1107 48-49 0.311936036118567 1107 50-51 0.3314582523107461 1107 52-53 0.3310043250556376 1107 54-55 0.28298781966316255 1107 56-57 0.2566366978423176 1107 58-59 0.21367378975970297 1107 60-61 0.16287705889924808 1107 62-63 0.09034341706250615 1107 64-65 -0.011118360597514254 1107 66-67 -10.389038087560309 1107 68-69 -8.405578682840119 1107 70-71 -6.868132304541245 1107 72-73 -1.8475756865461577 1107 74-75 -0.5493713480967486 1107 76-77 -0.23836360784543587 1107 78-79 2.847365221853785 1107 80-81 0.7905647189446441 1107 82-83 0.1266065036408932 1107 84-85 -5.584751890120227 1107 86-87 -6.047015659202206 1107 88-89 -1.544396179202412 1107 90-91 -0.28764087356738344 1107 92-93 0.09764166704653476 1107 94-95 0.19444759703858594 1107 96-97 0.28019005370528305 1107 98-99 0.3923484339439014 1107 100 0.498049739433192 1108 1 0.09507632921971165 1108 2 0.11642065903632215 1108 3 0.11062138637245766 1108 4 0.028792512969715744 1108 5 0.01744418766742939 1108 6 0.03369601675778 1108 7 -12.283196814114174 1108 8 -6.783753874511941 1108 9 -2.641935628434723 1108 10-11 -0.5418750030272435 1108 12-13 0.040317699993913436 1108 14-15 0.19991763983713895 1108 16-17 0.16096216130827656 1108 18-19 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-1.7989819082748753 2216 20-21 -1.3717779147689555 2216 22-23 -1.1297519357619166 2216 24-25 -1.1951966391742062 2216 26-27 -1.6342994168092346 2216 28-29 -1.801196409922035 2216 30-31 -1.70371211310718 2216 32-33 -2.607247890367887 2216 34-35 -1.721155863975845 2216 36-37 -1.398902297272194 2216 38-39 -1.625424504644215 2216 40-41 -1.5608143399718415 2216 42-43 -1.2876137036957402 2216 44-45 -1.3621661657052542 2216 46-47 -1.5716653734447235 2216 48-49 -1.6387446785650042 2216 50-51 -2.1237615731009285 2216 52-53 -1.919537022570502 2216 54-55 -1.513582500211264 2216 56-57 -1.5581870523194894 2216 58-59 -1.8401326664246227 2216 60-61 -2.0161599823297323 2216 62-63 -1.911542653010038 2216 64-65 -1.5919252661520815 2216 66-67 1.772050666718588 2216 68-69 0.9938184726144925 2216 70-71 0.6928441824738343 2216 72-73 -1.1450886137482783 2216 74-75 -1.9266904719888416 2216 76-77 -1.6128409780386264 2216 78-79 0.9465235920656241 2216 80-81 -0.48232289144014473 2216 82-83 -1.4013301864366703 2216 84-85 0.38809664819507006 2216 86-87 0.4220820130143643 2216 88-89 -0.8051493408576214 2216 90-91 -1.1123866696532048 2216 92-93 -1.2165263524044398 2216 94-95 -1.192563291806934 2216 96-97 -1.1460979196098329 2216 98-99 -1.1249726050133404 2216 100 -2.2969080255478396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98490.0 3 18784.0 4 11149.0 5 9975.0 6 9367.0 7 10414.0 8 11417.0 9 12797.0 10 14502.0 11 16397.0 12 18581.0 13 20811.0 14 23931.0 15 26272.0 16 27543.0 17 28076.0 18 27252.0 19 25533.0 20 24798.0 21 25172.0 22 27065.0 23 31265.0 24 37392.0 25 46745.0 26 58181.0 27 70036.0 28 87707.0 29 111462.0 30 137615.0 31 168341.0 32 223655.0 33 315504.0 34 480741.0 35 655379.0 36 731436.0 37 1232327.0 38 1392955.0 39 471119.0 40 1125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45482768980827 20.385354653729028 14.080659938204976 23.079157718257722 2 15.729077336860318 20.59290589127616 40.03890422005316 23.63911255181036 3 18.174790872826975 25.281947679304366 30.642332048157954 25.900929399710705 4 12.869383801947224 17.435801051831625 38.69539115702517 30.99942398919598 5 12.961273244175189 37.38239676734352 35.272358576841974 14.383971411639312 6 30.162071183508004 37.95872253717191 18.167907609308376 13.711298670011713 7 26.596185998989007 32.563790947308725 22.808022191454853 18.032000862247415 8 24.31122437627108 36.25080288549258 21.143917131833426 18.29405560640291 9 24.205321487170668 17.727412368306403 22.29429854222717 35.77296760229576 10-11 23.248104115060112 27.34945027754987 29.211061468607515 20.1913841387825 12-13 23.81511548718046 24.93022499629523 29.090150268990705 22.164509247533605 14-15 21.473679229455517 27.400211917236874 26.261894756079347 24.864214097228267 16-17 20.969512318587384 30.493985837887415 26.878449864948085 21.658051978577117 18-19 20.866542131048398 29.441647181089852 28.75614253666683 20.93566815119492 20-21 22.057358135335853 28.879132625622272 28.188384142816147 20.875125096225727 22-23 21.708071449905773 28.80867154835844 28.201215475833667 21.282041525902123 24-25 21.540646915711203 28.774877764873924 28.264383589482815 21.420091729932057 26-27 21.296331664243667 28.684202504279778 28.3376817778234 21.681784053653157 28-29 21.359213363691424 28.776516911918172 28.41797389261525 21.446295831775156 30-31 21.74328554193687 29.21659451700122 27.882247236479667 21.15787270458224 32-33 21.187174390969588 28.94404269674905 28.420346225017557 21.448436687263804 34-35 21.361221199390563 28.517289006419677 28.477815948747033 21.64367384544273 36-37 21.83947671286665 28.86392159623017 27.952963434324364 21.343638256578824 38-39 21.58839145593427 28.961401496044424 28.13665829514899 21.313548752872308 40-41 21.40131358732104 28.714483630445446 28.31143143458531 21.572771347648207 42-43 21.27359519143905 28.770276103605973 28.26861544714603 21.68751325780894 44-45 21.56038254924642 28.912196783351344 28.13615975074251 21.391260916659718 46-47 21.47333578243275 29.165784968626383 27.92492523831065 21.43595401063022 48-49 21.36558312271538 28.752436112224483 28.24696044100863 21.635020324051506 50-51 21.38413046233831 28.636240278469106 28.24892548328869 21.7307037759039 52-53 21.392760176565428 28.749957166996204 28.154240942062042 21.70304171437633 54-55 21.515550930983554 29.0988905749342 27.999122422440415 21.38643607164184 56-57 21.27716607069203 28.868056622275695 27.97906781709514 21.875709489937137 58-59 21.159327158401965 28.50631915451784 28.33975606595741 21.994597621122782 60-61 21.534206661446547 28.87144299547404 27.937087384804848 21.65726295827456 62-63 21.345949703432773 28.884805172303302 27.9969577827832 21.772287341480723 64-65 21.29083062401196 28.648876251570005 28.185852647431116 21.874440476986926 66-67 21.20187387455499 28.653482446306455 28.01902383531577 22.125619843822786 68-69 21.784173813365534 28.809581813666817 27.794509311009975 21.61173506195767 70-71 21.551201549706256 28.463317489593948 28.045384254626644 21.940096706073156 72-73 21.22281230497836 28.66949235889723 28.33258099883419 21.775114337290223 74-75 21.222498058555594 28.677689076658687 28.175880399607482 21.92393246517824 76-77 21.29276376372968 28.707091597141606 27.952245945264547 22.047898693864163 78-79 21.447365490947103 28.74443632800628 28.092971934722925 21.715226246323688 80-81 21.197151517587987 28.50640105990319 28.24124724651101 22.05520017599781 82-83 21.117305706184332 28.51340943082349 28.391068568144618 21.97821629484756 84-85 21.211276397234506 28.487745770342986 28.155963338155438 22.145014494267066 86-87 21.324228973271023 28.539773041239236 28.17274796333087 21.96325002215887 88-89 21.096720187728938 28.366024551382402 28.331253340461632 22.20600192042703 90-91 20.986392574860567 28.302766359016534 28.289675021832494 22.421166044290405 92-93 21.162467238167913 28.456310238899547 28.089536447890517 22.291686075042026 94-95 21.091799432653257 28.19632467008924 28.31107720596007 22.40079869129743 96-97 21.031985677138383 28.20011419482634 28.38690091292073 22.380999215114542 98-99 21.041249472070668 28.167208849660707 28.330360709804047 22.461180968464575 100 21.205509861247673 28.155720900314673 28.16617939477805 22.472589843659602 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3523.0 1 2782.0 2 2387.5 3 2708.5 4 2501.5 5 2127.5 6 1903.0 7 1862.0 8 1870.0 9 1855.0 10 1697.0 11 1531.5 12 1536.0 13 1650.0 14 1787.0 15 1998.5 16 2312.5 17 2798.5 18 3728.0 19 5118.0 20 7181.5 21 10240.0 22 15035.5 23 22244.0 24 31085.0 25 41996.5 26 56931.0 27 75027.0 28 96985.0 29 118292.5 30 135718.5 31 153271.5 32 170341.0 33 185766.5 34 199121.5 35 210388.5 36 219366.0 37 228498.5 38 235395.0 39 237375.5 40 237895.0 41 235981.0 42 230376.0 43 225172.0 44 221008.5 45 217269.0 46 213675.0 47 208707.5 48 204732.5 49 202981.0 50 199095.5 51 190708.0 52 180822.5 53 172749.5 54 166682.5 55 158253.5 56 145964.5 57 132114.0 58 118918.5 59 107240.5 60 93775.0 61 78516.5 62 65408.0 63 53925.5 64 43234.5 65 33812.5 66 25919.5 67 19832.0 68 15385.0 69 11864.5 70 8839.5 71 6626.0 72 5130.5 73 3793.5 74 2718.5 75 1899.5 76 1212.5 77 776.5 78 564.5 79 429.5 80 294.5 81 178.0 82 120.0 83 93.5 84 74.5 85 59.5 86 43.0 87 34.5 88 28.0 89 23.5 90 18.0 91 15.0 92 15.0 93 12.0 94 8.5 95 7.0 96 11.0 97 13.0 98 12.0 99 14.5 100 29.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019076408134856854 2 0.0020767473863763297 3 0.0011570449724096691 4 0.001750401368517192 5 0.001572394449684935 6 0.0029519480706349257 7 11.641415149071152 8 1.4833909902688067E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1509169358897696E-4 18-19 0.0 20-21 7.4169549513440335E-6 22-23 7.4169549513440335E-6 24-25 0.0 26-27 1.705899638809128E-4 28-29 0.0 30-31 0.0 32-33 2.22508648540321E-5 34-35 5.933563961075227E-5 36-37 3.708477475672017E-5 38-39 7.4169549513440335E-6 40-41 7.4169549513440335E-6 42-43 1.4833909902688067E-5 44-45 1.3350518912419261E-4 46-47 8.529498194045639E-4 48-49 5.488546663994585E-4 50-51 3.634307926158577E-4 52-53 2.0767473863763297E-4 54-55 3.9309861242123377E-4 56-57 0.0016688148640524078 58-59 3.1892906290779347E-4 60-61 0.001297967116485206 62-63 0.002595934232970412 64-65 0.0011570449724096691 66-67 5.821323181796538 68-69 5.467979447914508 70-71 0.0024105103591868108 72-73 0.001068041512993541 74-75 0.0029074463409268616 76-77 0.005881645276415819 78-79 5.409585761582576 80-81 0.004717183349054806 82-83 0.004583678159930613 84-85 5.821145174877706 86-87 0.0032412093137373426 88-89 0.001513058810074183 90-91 0.00314478889936987 92-93 0.003485968827131696 94-95 0.004205413457412067 96-97 0.0029667819805376133 98-99 0.0052215362857462 100 0.005622051853118777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6741311.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.47855806072106 #Duplication Level Percentage of deduplicated Percentage of total 1 92.38454449518323 81.74051284030337 2 5.404216716654394 9.563146050744502 3 1.1789639809954118 3.1293909913200406 4 0.4353378844001502 1.5407227312372065 5 0.22005823477907102 0.9735217651319911 6 0.12944099906870715 0.6871651770923008 7 0.0687011478301377 0.4255004947988913 8 0.049738724215739154 0.35206484787107756 9 0.03165215843422826 0.25204836039930056 >10 0.09614766385375897 1.2471836808961716 >50 9.745725352087912E-4 0.055689091545486055 >100 2.059187595054046E-4 0.022383660583597767 >500 1.7503290487276863E-5 0.010670308076025805 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.4833909902688067E-5 5 0.0 2.9667819805376134E-5 0.0 1.4833909902688067E-5 1.4833909902688067E-5 6 0.0 2.9667819805376134E-5 0.0 1.4833909902688067E-5 1.4833909902688067E-5 7 0.0 2.9667819805376134E-5 0.0 1.4833909902688067E-5 1.4833909902688067E-5 8 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 1.4833909902688067E-5 9 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 1.4833909902688067E-5 10-11 0.0 5.933563961075227E-5 0.0 2.9667819805376134E-5 1.4833909902688067E-5 12-13 0.0 7.416954951344034E-5 0.0 4.45017297080642E-5 1.4833909902688067E-5 14-15 0.0 1.112543242701605E-4 0.0 5.9335639610752275E-5 1.4833909902688067E-5 16-17 0.0 1.4092214407553663E-4 0.0 8.90034594161284E-5 1.4833909902688067E-5 18-19 0.0 1.6317300892956875E-4 0.0 1.112543242701605E-4 1.4833909902688067E-5 20-21 0.0 1.6317300892956875E-4 0.0 1.2608823417284857E-4 1.4833909902688067E-5 22-23 0.0 1.6317300892956875E-4 0.0 3.1151210795644945E-4 1.4833909902688067E-5 24-25 0.0 1.780069188322568E-4 0.0 7.416954951344034E-4 1.4833909902688067E-5 26-27 0.0 2.0767473863763294E-4 0.0 0.0012386314768744536 1.4833909902688067E-5 28-29 0.0 2.0767473863763294E-4 0.0 0.0031522058543212144 1.4833909902688067E-5 30-31 0.0 2.0767473863763294E-4 0.0 0.008774257707439993 1.4833909902688067E-5 32-33 0.0 2.0767473863763294E-4 0.0 0.0210715690167684 1.4833909902688067E-5 34-35 0.0 2.22508648540321E-4 0.0 0.041586866412186 1.4833909902688067E-5 36-37 0.0 2.3734255844300907E-4 0.0 0.07263423983851212 1.4833909902688067E-5 38-39 0.0 2.7442733319972925E-4 0.0 0.11557840900679407 1.4833909902688067E-5 40-41 0.0 3.337629728104815E-4 0.0 0.1643745556316865 1.4833909902688067E-5 42-43 0.0 3.4117992776182553E-4 0.0 0.2167456745431267 1.4833909902688067E-5 44-45 0.0 3.4117992776182553E-4 0.0 0.28062790753905287 1.4833909902688067E-5 46-47 0.0 3.4859688271316955E-4 0.0 0.36001157638328807 1.4833909902688067E-5 48-49 0.0 3.634307926158577E-4 0.0 0.44492087666627456 1.4833909902688067E-5 50-51 0.0 3.708477475672017E-4 0.0 0.5360233343336334 1.4833909902688067E-5 52-53 0.0 3.708477475672017E-4 0.0 0.6297217262339625 1.4833909902688067E-5 54-55 0.0 3.708477475672017E-4 0.0 0.7337148516067572 1.4833909902688067E-5 56-57 0.0 3.7826470251854573E-4 0.0 0.8461336378042787 1.4833909902688067E-5 58-59 1.4833909902688067E-5 4.0051556737257784E-4 0.0 0.9720512820132463 1.4833909902688067E-5 60-61 1.4833909902688067E-5 4.0051556737257784E-4 0.0 1.1058457323805415 1.4833909902688067E-5 62-63 1.4833909902688067E-5 4.153494772752659E-4 0.0 1.2389652398472641 1.4833909902688067E-5 64-65 1.4833909902688067E-5 4.153494772752659E-4 0.0 1.3896703475036236 1.4833909902688067E-5 66-67 1.4833909902688067E-5 4.153494772752659E-4 0.0 1.565103582967764 1.4833909902688067E-5 68-69 1.4833909902688067E-5 4.153494772752659E-4 0.0 1.7409744187740337 1.4833909902688067E-5 70-71 1.4833909902688067E-5 4.30183387177954E-4 0.0 1.954389880544007 1.4833909902688067E-5 72-73 1.4833909902688067E-5 4.895190267887062E-4 0.0 2.1933864199411657 1.4833909902688067E-5 74-75 1.4833909902688067E-5 4.895190267887062E-4 0.0 2.4116080685196097 1.4833909902688067E-5 76-77 1.4833909902688067E-5 5.043529366913943E-4 0.0 2.6418006823895235 1.4833909902688067E-5 78-79 1.4833909902688067E-5 5.117698916427384E-4 0.0 2.916999378904192 1.4833909902688067E-5 80-81 1.4833909902688067E-5 5.191868465940824E-4 0.0 3.2498203984358534 1.4833909902688067E-5 82-83 1.4833909902688067E-5 5.191868465940824E-4 0.0 3.5986249558876606 1.4833909902688067E-5 84-85 1.4833909902688067E-5 5.488546663994585E-4 0.0 3.9553953229572114 1.4833909902688067E-5 86-87 1.4833909902688067E-5 5.562716213508025E-4 0.0 4.371115647980044 1.4833909902688067E-5 88 1.4833909902688067E-5 5.636885763021465E-4 0.0 4.72460030400615 1.4833909902688067E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6095 0.0 43.096237 1 GTATCAA 9620 0.0 32.999874 1 ATCAACG 10050 0.0 30.36085 3 TCAACGC 10020 0.0 30.349562 4 CAACGCA 10870 0.0 28.117615 5 TATCAAC 11720 0.0 26.733604 2 AACGCAG 12020 0.0 25.511606 6 GTACATG 16085 0.0 23.301598 1 TACATGG 16380 0.0 22.097301 2 CGCAGAG 14450 0.0 21.16092 8 ACGCAGA 14305 0.0 21.006554 7 ACATGGG 16845 0.0 20.271624 3 GTGGTAT 3880 0.0 19.399088 1 GCAGAGT 16785 0.0 17.300928 9 CATGGGG 11415 0.0 16.77372 4 TGGTATC 4230 0.0 16.581121 2 TATACCG 535 1.8189894E-12 16.267801 5 AGAGTAC 14005 0.0 16.148886 10-11 GTATAGG 2190 0.0 16.132462 1 CAGAGTA 15735 0.0 15.94008 10-11 >>END_MODULE