FastQCFastQC Report
Wed 25 May 2016
SRR2049494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6741311
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231210.3429748308600508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222860.33058851609130624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138560.20553865561164586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127800.058.2464561
GTATCAA226700.038.5889241
GTGGTAT50800.033.4589271
ATCAACG282900.030.6382433
TCAACGC285750.030.3985354
TGGTATC54500.030.0462272
TATCAAC290050.029.915432
CAACGCA296100.029.3648665
AACGCAG314300.027.8877246
ACGCAGA360850.024.224527
CGCAGAG360450.024.029758
GTACATG170500.022.8375591
GCAGAGT385850.022.155589
TACATGG174750.021.4878462
GAGTACT295600.020.7797412-13
CAGAGTA376650.020.55060410-11
ACATGGG176600.020.3301723
AGAGTAC359650.019.9277710-11
ATAACGC5800.019.47113
AGTACTT310700.018.72614312-13
Produced by FastQC (version 0.11.5)