##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6741311 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.758690260692617 31.0 31.0 34.0 30.0 34.0 2 31.80511728356695 31.0 31.0 34.0 30.0 34.0 3 31.830764075415004 33.0 31.0 34.0 30.0 34.0 4 35.54383175616731 37.0 35.0 37.0 33.0 37.0 5 35.68257272213076 37.0 35.0 37.0 33.0 37.0 6 35.76975264900255 37.0 35.0 37.0 33.0 37.0 7 35.7871227124813 37.0 35.0 37.0 33.0 37.0 8 35.79096232172051 37.0 35.0 37.0 33.0 37.0 9 37.524124016826995 39.0 37.0 39.0 35.0 39.0 10-11 37.49169078536801 39.0 37.0 39.0 34.5 39.0 12-13 37.480466707440144 39.0 37.0 39.0 34.5 39.0 14-15 38.75675658636725 40.0 38.0 41.0 35.0 41.0 16-17 38.770172522822335 40.0 38.0 41.0 35.0 41.0 18-19 38.84705919961266 40.0 38.0 41.0 35.0 41.0 20-21 38.87274433711781 40.0 38.0 41.0 35.0 41.0 22-23 38.84495233938918 40.0 38.0 41.0 35.0 41.0 24-25 38.80569076252379 40.0 38.0 41.0 35.0 41.0 26-27 38.711717794951156 40.0 38.0 41.0 34.5 41.0 28-29 38.6235962856483 40.0 38.0 41.0 34.0 41.0 30-31 38.497462808050244 40.0 38.0 41.0 34.0 41.0 32-33 38.3411833395611 40.0 38.0 41.0 34.0 41.0 34-35 38.228331403194424 40.0 38.0 41.0 34.0 41.0 36-37 38.081089568483044 40.0 38.0 41.0 33.5 41.0 38-39 37.98641673704121 40.0 38.0 41.0 33.0 41.0 40-41 37.86125183959025 40.0 37.0 41.0 33.0 41.0 42-43 37.71534720768705 40.0 37.0 41.0 33.0 41.0 44-45 37.528342187446924 40.0 37.0 41.0 33.0 41.0 46-47 37.271819160991086 40.0 37.0 41.0 32.0 41.0 48-49 37.0320809260988 40.0 37.0 41.0 31.0 41.0 50-51 36.819755979215316 40.0 36.5 41.0 31.0 41.0 52-53 36.7185403551327 40.0 36.0 41.0 31.0 41.0 54-55 36.59053787905646 40.0 36.0 41.0 31.0 41.0 56-57 36.42321938566549 39.0 35.5 41.0 30.5 41.0 58-59 36.25637987922527 39.0 35.0 41.0 30.0 41.0 60-61 36.06960040858522 39.0 35.0 41.0 30.0 41.0 62-63 35.86820330644885 39.0 35.0 41.0 30.0 41.0 64-65 35.59580287869822 38.0 35.0 40.0 29.0 41.0 66-67 35.344309066886254 37.5 35.0 40.0 29.0 41.0 68-69 35.03930303764357 37.0 34.5 40.0 29.0 41.0 70-71 34.74824963571626 37.0 34.0 39.5 29.0 41.0 72-73 34.43126003235869 36.0 34.0 39.0 28.0 41.0 74-75 34.097323057785054 36.0 34.0 39.0 28.0 41.0 76-77 33.806962176941546 35.0 34.0 38.5 28.0 40.0 78-79 33.48390906457216 35.0 34.0 37.0 28.0 39.0 80-81 33.177918508729235 35.0 34.0 37.0 27.0 39.0 82-83 32.885444018233244 35.0 34.0 36.5 27.0 39.0 84-85 32.61168569437013 35.0 33.0 36.0 26.5 37.5 86-87 32.33459196586539 35.0 33.0 36.0 26.0 37.0 88-89 32.123173667555164 35.0 33.0 35.0 26.0 37.0 90-91 31.972714580294546 35.0 33.0 35.0 26.0 36.0 92-93 31.81390837776213 35.0 33.0 35.0 26.0 36.0 94-95 31.632126080520543 35.0 33.0 35.0 25.0 36.0 96-97 31.464793717423806 35.0 33.0 35.0 25.0 36.0 98-99 31.28603123339066 35.0 33.0 35.0 25.0 35.0 100 31.176514182478748 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3565636789215212 1101 2 0.36848544704460906 1101 3 0.4611031522468778 1101 4 0.3637691678940982 1101 5 0.27113083298494445 1101 6 0.24906882672947006 1101 7 0.3082499194755286 1101 8 0.3138642300622081 1101 9 0.341897230255924 1101 10-11 0.35266092163556095 1101 12-13 0.28694579163403944 1101 14-15 0.45972102291786143 1101 16-17 0.46226219373959765 1101 18-19 0.45698138340998895 1101 20-21 0.41213768412539764 1101 22-23 0.4469657182605147 1101 24-25 0.46114757952033614 1101 26-27 0.5146126458255011 1101 28-29 0.48816237718232003 1101 30-31 0.5235549864061468 1101 32-33 0.5432150930221695 1101 34-35 0.5784712076742622 1101 36-37 0.5957968574266062 1101 38-39 0.5934614209625835 1101 40-41 0.6241433190068264 1101 42-43 0.5885173661345249 1101 44-45 0.6251131815898532 1101 46-47 0.7074113078121727 1101 48-49 0.6974132715082249 1101 50-51 0.7286930063020876 1101 52-53 0.680970957492633 1101 54-55 0.607268420983587 1101 56-57 0.634656728422371 1101 58-59 0.6232800356045303 1101 60-61 0.6394689218699696 1101 62-63 0.6430022445256469 1101 64-65 0.6236285668852801 1101 66-67 0.6455460818861383 1101 68-69 0.6567210381048767 1101 70-71 0.6690942067610166 1101 72-73 0.6535849432591192 1101 74-75 0.6536140969808955 1101 76-77 0.6378385484931641 1101 78-79 0.6020521061698432 1101 80-81 0.5778211122150623 1101 82-83 0.6409604086708072 1101 84-85 0.6226849599264526 1101 86-87 0.6692931943343652 1101 88-89 0.6722695420896798 1101 90-91 0.6677698844407871 1101 92-93 0.6389462563890724 1101 94-95 0.6061967569617615 1101 96-97 0.6407972630973191 1101 98-99 0.6862468715485193 1101 100 0.7430102646287189 1102 1 0.2953532804612884 1102 2 0.34866968351380123 1102 3 0.44302680508926784 1102 4 0.3572309954121238 1102 5 0.2798308653194468 1102 6 0.2628088296865201 1102 7 0.2798998982628689 1102 8 0.29496883722432443 1102 9 0.38174137751347814 1102 10-11 0.35032734780430985 1102 12-13 0.33881867557774115 1102 14-15 0.441906126759001 1102 16-17 0.43392890464501477 1102 18-19 0.4253125951114711 1102 20-21 0.4057479893113367 1102 22-23 0.39119348302503454 1102 24-25 0.3969049165349148 1102 26-27 0.39238651401918645 1102 28-29 0.43683053677875705 1102 30-31 0.4812534608055117 1102 32-33 0.5106302281141453 1102 34-35 0.4665880256081891 1102 36-37 0.48701238575351624 1102 38-39 0.4577304846089163 1102 40-41 0.43875510864040024 1102 42-43 0.46308122326779966 1102 44-45 0.4698415224086716 1102 46-47 0.4419036506333711 1102 48-49 0.4946623709023825 1102 50-51 0.5290060905003529 1102 52-53 0.4547943018629894 1102 54-55 0.47022527745569676 1102 56-57 0.45827990708388455 1102 58-59 0.4921148560909998 1102 60-61 0.4604022987547012 1102 62-63 0.4336299989360981 1102 64-65 0.437414373398596 1102 66-67 0.4392989308980475 1102 68-69 0.45315100955662757 1102 70-71 0.4322715257525118 1102 72-73 0.49321951401969955 1102 74-75 0.4366593117596551 1102 76-77 0.3955777536171894 1102 78-79 0.3474908572946447 1102 80-81 0.35230369984397925 1102 82-83 0.35137198148332516 1102 84-85 0.3170057521963585 1102 86-87 0.3433814666496673 1102 88-89 0.35573199589674687 1102 90-91 0.36314464110940037 1102 92-93 0.4002788689598695 1102 94-95 0.4065901619077543 1102 96-97 0.4092824914504263 1102 98-99 0.46078748140269 1102 100 0.4774986533833321 1103 1 0.27405329721289107 1103 2 0.2954712866612432 1103 3 0.395951937789242 1103 4 0.3116532652461288 1103 5 0.23201489198940806 1103 6 0.24135544162284361 1103 7 0.26609637168350986 1103 8 0.26063082936908444 1103 9 0.2748817056807624 1103 10-11 0.245743459079236 1103 12-13 0.2570368012218367 1103 14-15 0.3939391057606443 1103 16-17 0.37091277402245737 1103 18-19 0.34629475567364665 1103 20-21 0.35099333638780195 1103 22-23 0.33717621543316767 1103 24-25 0.3823593378957142 1103 26-27 0.37151052125864936 1103 28-29 0.34974682584386585 1103 30-31 0.39983154815854505 1103 32-33 0.3837676274999069 1103 34-35 0.4188933977807636 1103 36-37 0.42006005225413645 1103 38-39 0.46427798879406623 1103 40-41 0.5081091106362194 1103 42-43 0.4461756912912307 1103 44-45 0.4578603928181906 1103 46-47 0.49764637622417496 1103 48-49 0.5290024610802675 1103 50-51 0.6205970469455977 1103 52-53 0.5657398078386393 1103 54-55 0.5080955175937873 1103 56-57 0.5886817955666004 1103 58-59 0.5643761187505731 1103 60-61 0.5185498903170753 1103 62-63 0.5191148824575293 1103 64-65 0.524432660292689 1103 66-67 0.4832513606705007 1103 68-69 0.44628350417409024 1103 70-71 0.48724082703157734 1103 72-73 0.4592942869915646 1103 74-75 0.44138547597250266 1103 76-77 0.4783908216314714 1103 78-79 0.4508349871361901 1103 80-81 0.3898704863791451 1103 82-83 0.4211243730256058 1103 84-85 0.45101079877399997 1103 86-87 0.46781744260617586 1103 88-89 0.526479488209489 1103 90-91 0.5072354015837774 1103 92-93 0.4740101562783643 1103 94-95 0.47146323810587987 1103 96-97 0.5342054507974794 1103 98-99 0.5599515493588783 1103 100 0.5325955365627912 1104 1 0.2657639138454222 1104 2 0.2757266386419168 1104 3 0.36246805175616004 1104 4 0.2939058044760898 1104 5 0.19202861306924746 1104 6 0.21983122183262083 1104 7 0.15658752911170382 1104 8 0.21945105672779874 1104 9 0.25134715692730225 1104 10-11 0.25603691849765653 1104 12-13 0.24368407797255998 1104 14-15 0.31403699033329957 1104 16-17 0.3222985006373591 1104 18-19 0.32276101944550817 1104 20-21 0.33015937452491784 1104 22-23 0.34137326200959706 1104 24-25 0.332456646635201 1104 26-27 0.33272338840969695 1104 28-29 0.33560920313045983 1104 30-31 0.34291743455831636 1104 32-33 0.32072929856069265 1104 34-35 0.3552428839998072 1104 36-37 0.35211611932774645 1104 38-39 0.4430654254568367 1104 40-41 0.4508322927962709 1104 42-43 0.3430391669000983 1104 44-45 0.40638080186462844 1104 46-47 0.42768593207663486 1104 48-49 0.46650032820129184 1104 50-51 0.5375138595429902 1104 52-53 0.5292277105729966 1104 54-55 0.43381547685447686 1104 56-57 0.4386742885391399 1104 58-59 0.4568906762015885 1104 60-61 0.482167231868452 1104 62-63 0.44084558966190457 1104 64-65 0.48117314076363726 1104 66-67 0.5243618213688279 1104 68-69 0.4416479412123806 1104 70-71 0.49470973645655647 1104 72-73 0.4463226734469714 1104 74-75 0.48613343591571123 1104 76-77 0.4225253956085453 1104 78-79 0.43224461965924377 1104 80-81 0.423847292667098 1104 82-83 0.4392721614906421 1104 84-85 0.48178798631297326 1104 86-87 0.4905470260527025 1104 88-89 0.4081286390675345 1104 90-91 0.46433787975068697 1104 92-93 0.4804575094064596 1104 94-95 0.4759303257077754 1104 96-97 0.485442414623499 1104 98-99 0.5328229184746505 1104 100 0.5614042931835463 1105 1 0.23039450754440693 1105 2 0.21920643268910922 1105 3 0.3104294518688988 1105 4 0.26322927064769175 1105 5 0.19959597075617097 1105 6 0.21146725295211155 1105 7 0.22143858171065745 1105 8 0.2218103039343191 1105 9 0.21994679109046444 1105 10-11 0.23449059294189567 1105 12-13 0.26518297319096007 1105 14-15 0.32702630953435374 1105 16-17 0.29853966151900835 1105 18-19 0.2914071086152248 1105 20-21 0.2800652087733937 1105 22-23 0.2707112762155788 1105 24-25 0.23460103821725653 1105 26-27 0.28251204153783505 1105 28-29 0.3238422416521729 1105 30-31 0.36947391745299285 1105 32-33 0.3334785644657643 1105 34-35 0.3227273980629377 1105 36-37 0.34167175519637283 1105 38-39 0.34760111849252695 1105 40-41 0.33366305685628106 1105 42-43 0.34575484387502087 1105 44-45 0.4007655005237396 1105 46-47 0.38548088564673577 1105 48-49 0.42449011385430424 1105 50-51 0.44060513641341714 1105 52-53 0.4101921596186173 1105 54-55 0.38738155207891367 1105 56-57 0.4212358710484949 1105 58-59 0.4300846354121788 1105 60-61 0.4249422128580349 1105 62-63 0.4069865026290955 1105 64-65 0.4557384736150283 1105 66-67 0.3670573324038955 1105 68-69 0.43439008525187717 1105 70-71 0.480463893694413 1105 72-73 0.45848997408679537 1105 74-75 0.47336561921860465 1105 76-77 0.43901746424276666 1105 78-79 0.42221659499137587 1105 80-81 0.44753139706202205 1105 82-83 0.4270830719604959 1105 84-85 0.4382964882960394 1105 86-87 0.5117829720843332 1105 88-89 0.45892242911675396 1105 90-91 0.39142846842769785 1105 92-93 0.41110014073585077 1105 94-95 0.41741119876344257 1105 96-97 0.4530191413381992 1105 98-99 0.4938605982835895 1105 100 0.5410114816252154 1106 1 0.15397973725288594 1106 2 0.18066217284573938 1106 3 0.315749664968088 1106 4 0.2058599233988474 1106 5 0.1803468376244055 1106 6 0.20726353400360864 1106 7 0.18449929988061342 1106 8 0.19654135576931964 1106 9 0.1885948621192597 1106 10-11 0.21838279303865704 1106 12-13 0.19148111252917488 1106 14-15 0.23250841437963743 1106 16-17 0.3043012056365839 1106 18-19 0.2142263614334965 1106 20-21 0.21553226721488272 1106 22-23 0.23021321287045282 1106 24-25 0.23660615566276277 1106 26-27 0.22806924786706162 1106 28-29 0.2796356631847985 1106 30-31 0.22489399458365966 1106 32-33 0.25804253378333186 1106 34-35 0.26897636951029824 1106 36-37 0.2679944982723228 1106 38-39 0.24501171764702434 1106 40-41 0.31357302348124705 1106 42-43 0.2616584106064508 1106 44-45 0.247579750032223 1106 46-47 0.20916003933471217 1106 48-49 0.2544459868786575 1106 50-51 0.3218055905246189 1106 52-53 0.3903963909054369 1106 54-55 0.26928666459047435 1106 56-57 0.32344802557769015 1106 58-59 0.33403410317080073 1106 60-61 0.3193666694163255 1106 62-63 0.3011167934871182 1106 64-65 0.35512721611144116 1106 66-67 0.34698225165475804 1106 68-69 0.38010937653793064 1106 70-71 0.3970455217191642 1106 72-73 0.3839333915454972 1106 74-75 0.3548182934507409 1106 76-77 0.32232176648506083 1106 78-79 0.33390281434880364 1106 80-81 0.26119387949103867 1106 82-83 0.255924331104481 1106 84-85 0.27813523178914323 1106 86-87 0.3387399223292249 1106 88-89 0.33717469947248446 1106 90-91 0.302244739957203 1106 92-93 0.2860723101432221 1106 94-95 0.2662731097787763 1106 96-97 0.25450486591591925 1106 98-99 0.320733214398885 1106 100 0.3477033212944214 1107 1 0.1373932609765589 1107 2 0.1335074867794397 1107 3 0.23161652590201953 1107 4 0.19254358227354373 1107 5 0.1817864618591969 1107 6 0.1804257664370823 1107 7 0.1457250507362815 1107 8 0.16840733515122963 1107 9 0.13043640799467227 1107 10-11 0.14308688805004977 1107 12-13 0.12375779154736222 1107 14-15 0.1619965078673502 1107 16-17 0.1451466306795126 1107 18-19 0.15369963318723734 1107 20-21 0.21825192200380172 1107 22-23 0.18607090958058592 1107 24-25 0.21352804454355123 1107 26-27 0.22593322774036295 1107 28-29 0.2675393591181745 1107 30-31 0.24840615932882315 1107 32-33 0.2464998310219073 1107 34-35 0.24942448019339736 1107 36-37 0.34309780629353526 1107 38-39 0.2690194533758188 1107 40-41 0.26033050318516615 1107 42-43 0.265892482436378 1107 44-45 0.27152033972297573 1107 46-47 0.2939091482299503 1107 48-49 0.34717443201886056 1107 50-51 0.41272650623860585 1107 52-53 0.3478591767480168 1107 54-55 0.33533005787199954 1107 56-57 0.3510088268802818 1107 58-59 0.3823679748896964 1107 60-61 0.2781865529721941 1107 62-63 0.3696412541531444 1107 64-65 0.33891474962783263 1107 66-67 0.3041734330777004 1107 68-69 0.3403632693767378 1107 70-71 0.3623441078816114 1107 72-73 0.37046933645838465 1107 74-75 0.3135871939758985 1107 76-77 0.2797072883934604 1107 78-79 0.21589342598454664 1107 80-81 0.22989442175261132 1107 82-83 0.2567350303929743 1107 84-85 0.29667464964355617 1107 86-87 0.31823604927088667 1107 88-89 0.2736127305161631 1107 90-91 0.25853946022300534 1107 92-93 0.25223822560986875 1107 94-95 0.28025172234934104 1107 96-97 0.3161599889828963 1107 98-99 0.33453693975241094 1107 100 0.316257449224441 1108 1 0.15571631547896558 1108 2 0.16567740210156146 1108 3 0.20299432735933465 1108 4 0.16625865432907005 1108 5 0.16884221043981285 1108 6 0.20309714368249843 1108 7 0.12130383363994213 1108 8 0.16968115896397507 1108 9 0.18012636055448183 1108 10-11 0.20088915944893415 1108 12-13 0.18981116656189556 1108 14-15 0.21702397447680966 1108 16-17 0.2299239929957011 1108 18-19 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-0.7867402162290986 2215 38-39 -0.7062665846758449 2215 40-41 -0.7069115593814672 2215 42-43 -0.6976501545516953 2215 44-45 -0.7740807718198042 2215 46-47 -0.6870779490516057 2215 48-49 -0.6420533026742561 2215 50-51 -0.6445760975927897 2215 52-53 -0.7769283311459532 2215 54-55 -0.7193491212423879 2215 56-57 -0.59277572539537 2215 58-59 -0.597130352601944 2215 60-61 -0.7663901434731528 2215 62-63 -0.7277755421252721 2215 64-65 -0.7030048783106935 2215 66-67 -0.5850041965921307 2215 68-69 -0.5576990909797956 2215 70-71 -0.6611024790472158 2215 72-73 -0.5004088955648953 2215 74-75 -0.6110564683937199 2215 76-77 -0.6097136945719939 2215 78-79 -0.5955521134609327 2215 80-81 -0.5731242696686891 2215 82-83 -0.5961370121998897 2215 84-85 -0.5621917597739241 2215 86-87 -0.5441215172087439 2215 88-89 -0.5186532276298195 2215 90-91 -0.5078499101182956 2215 92-93 -0.6283857738134735 2215 94-95 -0.6592229010323436 2215 96-97 -0.7529580943673793 2215 98-99 -0.7440668496786884 2215 100 -0.8470490924169063 2216 1 -0.5970690911210355 2216 2 -0.9870002688824506 2216 3 -0.6193018361412932 2216 4 -1.1209971891845925 2216 5 -0.5862742968528636 2216 6 -0.8430653073339442 2216 7 -0.5859473494084071 2216 8 -0.566395820486207 2216 9 -0.6041852922793325 2216 10-11 -0.8554786353043653 2216 12-13 -0.6258985201075546 2216 14-15 -0.8957454241298919 2216 16-17 -0.8295648502581301 2216 18-19 -0.9316878307296363 2216 20-21 -0.7395610737733733 2216 22-23 -0.7103174229678544 2216 24-25 -0.7795079821428672 2216 26-27 -0.910105931724253 2216 28-29 -1.189955497218392 2216 30-31 -1.2758169644020967 2216 32-33 -0.9727304687232987 2216 34-35 -1.0438882106765632 2216 36-37 -1.001513382418473 2216 38-39 -1.0996960674653664 2216 40-41 -0.9589837299128163 2216 42-43 -1.4708561947656946 2216 44-45 -1.1713675603452671 2216 46-47 -0.9907144869798259 2216 48-49 -0.9882214858253349 2216 50-51 -1.084875166928832 2216 52-53 -0.9967519763215975 2216 54-55 -1.1979411873240906 2216 56-57 -1.3290930260361407 2216 58-59 -1.1124941045543792 2216 60-61 -1.3030145573200613 2216 62-63 -1.3210540580459167 2216 64-65 -1.325345637499936 2216 66-67 -1.149703102265768 2216 68-69 -1.4236805261280594 2216 70-71 -1.2187573240359129 2216 72-73 -1.0529869565858618 2216 74-75 -1.1498430188250666 2216 76-77 -1.2376594530072609 2216 78-79 -1.2982155839398715 2216 80-81 -1.081849162821463 2216 82-83 -1.1599954873722282 2216 84-85 -1.1999624471233759 2216 86-87 -1.352594311376464 2216 88-89 -1.1502767021121052 2216 90-91 -1.0726683887069797 2216 92-93 -1.1311318296234418 2216 94-95 -1.2565797244817567 2216 96-97 -1.376791641890847 2216 98-99 -1.3398612232677678 2216 100 -1.3334004146561789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 434.0 3 36.0 4 50.0 5 124.0 6 449.0 7 1123.0 8 2375.0 9 4132.0 10 6119.0 11 8764.0 12 12801.0 13 18322.0 14 21028.0 15 25924.0 16 32820.0 17 46393.0 18 46785.0 19 24929.0 20 18091.0 21 17546.0 22 18203.0 23 19752.0 24 23109.0 25 28050.0 26 36045.0 27 46952.0 28 61125.0 29 80528.0 30 105009.0 31 135693.0 32 174672.0 33 228621.0 34 311625.0 35 453138.0 36 730473.0 37 1347093.0 38 1879149.0 39 771117.0 40 2712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.69211818529002 20.741588753627372 14.633817991993551 22.932475069089055 2 15.78025791156398 21.13408935928262 39.34724488722729 23.738407841926108 3 18.032056375977906 25.531843880218553 31.07189387939527 25.364205864408273 4 12.822945800353109 17.85736562959654 38.02560932721531 31.294079242835043 5 12.408284215020279 37.31891593284644 35.397063967485934 14.875735884647344 6 29.745129522395626 38.3324076859587 17.63058600005815 14.291876791587526 7 25.75312131423695 33.0785955432111 22.81560663793734 18.352676504614607 8 24.55816086811601 35.7379150731957 20.87869852021365 18.825225538474637 9 24.238612376526227 16.808706319691574 22.43685574465497 36.51582555912723 10-11 23.351422297532334 27.09340215871957 28.95304043976016 20.602135103987933 12-13 24.360688056178994 24.659153784650165 29.315110861242104 21.66504729792874 14-15 21.615708103046135 26.726064613104526 27.354369747543895 24.30385753630544 16-17 20.313363379912307 29.91374378069785 28.013223243965452 21.75966959542439 18-19 20.11302400972155 27.924553547522134 31.307753046848006 20.654669395908304 20-21 21.260078343811763 27.30671378312023 31.110595550331382 20.32261232273663 22-23 20.839922679728023 27.249009873598773 31.256538972908977 20.654528473764227 24-25 20.75855868391178 27.21000410750965 31.26447511470692 20.76696209387165 26-27 20.554814931398358 27.136568836536394 31.310311896306224 20.998304335759023 28-29 20.63831901858692 27.230230074318012 31.36513728806017 20.766313619034904 30-31 20.98222537097477 27.661425239724007 30.884315385147126 20.4720340041541 32-33 20.514468218011913 27.35734394669998 31.346612798802248 20.781575036485858 34-35 20.67226241305289 27.054900745567146 31.27118745893788 21.001649382442082 36-37 21.198799462003755 27.39959630997591 30.65675949381359 20.744844734206744 38-39 20.979368849768242 27.505718101419742 30.76307412608616 20.751838922725863 40-41 20.812606034642222 27.332621206765275 30.865324267045384 20.98944849154712 42-43 20.81049220248109 27.403097112712942 30.61393399592453 21.172476688881435 44-45 21.112117509487398 27.602605783949148 30.34099005371507 20.944286652848383 46-47 21.165638256416297 27.945669618268614 29.791430776595234 21.097261348719858 48-49 21.17383399163753 27.714075199912898 29.764981915238742 21.34710889321083 50-51 21.24772169686282 27.68296849084696 29.53246779446906 21.53684201782116 52-53 21.22116158118206 27.881935724371715 29.393703984284365 21.50319871016187 54-55 21.376531953502813 28.258598364620767 29.20887346689687 21.15599621497955 56-57 21.062868928610474 27.98952607289591 29.238726710576028 21.70887828791759 58-59 20.940842270778635 27.60184237461617 29.64928871285954 21.808026641745656 60-61 21.312545883137567 27.885162099775545 29.312859175314713 21.48943284177217 62-63 21.121329367536966 27.84506604130858 29.415888096543835 21.61771649461062 64-65 21.03943135096423 27.653426759275757 29.602780527407802 21.70436136235222 66-67 21.00019565927162 27.714846563227834 29.3858939307206 21.89906384677995 68-69 21.451598954565366 27.934151087229175 29.10355270658778 21.510697251617675 70-71 21.361423616266926 27.59337909198967 29.209741250626177 21.835456041117222 72-73 21.131357090631184 27.762530166610027 29.4245362660171 21.68157647674169 74-75 21.215865875346797 27.76646856968919 29.193283027589146 21.82438252737487 76-77 21.230469859645996 27.854092475484364 28.992179711038403 21.923257953831236 78-79 21.304336152424035 27.908768028239066 29.100311379157045 21.68658444017985 80-81 21.16459749064367 27.689668617603708 29.182713606541654 21.96302028521097 82-83 21.010437171520586 27.73558176550779 29.314678644591762 21.939302418379867 84-85 21.086046912833424 27.69489495440872 29.072846513089218 22.146211619668637 86-87 21.187221595324708 27.76624606104065 29.022670812843376 22.023861530791265 88-89 20.998754395846383 27.64202846718003 29.110897931217195 22.248319205756392 90-91 20.788876697055926 27.569735364373706 29.06425267385093 22.577135264719438 92-93 20.95131911902826 27.74419561445519 28.877129136309154 22.4273561302074 94-95 20.833848197288845 27.509199685578206 29.096090747386654 22.560861369746295 96-97 20.740498398605254 27.50819536437349 29.13471133433838 22.616594902682877 98-99 20.731745125464503 27.465763696635182 29.069265331110028 22.73322584679029 100 20.839436939800184 27.488710151392187 28.908005404945953 22.76384750386168 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 978.0 1 881.0 2 2519.5 3 4037.5 4 2479.5 5 1231.0 6 2333.5 7 5620.5 8 9115.0 9 10274.5 10 8034.0 11 5287.5 12 4949.5 13 5507.5 14 6197.5 15 6872.5 16 7704.0 17 8597.5 18 9580.5 19 10931.5 20 12811.5 21 15310.0 22 19310.5 23 25483.5 24 33251.5 25 42820.0 26 56212.0 27 73194.5 28 93868.0 29 114504.0 30 130944.0 31 147364.0 32 164452.0 33 179892.0 34 192926.0 35 204379.0 36 213768.5 37 221708.5 38 229166.5 39 232451.5 40 233291.0 41 231804.5 42 226317.5 43 222171.0 44 218676.5 45 215668.5 46 212659.0 47 207477.0 48 203134.0 49 201324.5 50 198437.0 51 190235.5 52 179210.5 53 171671.5 54 165901.0 55 157128.5 56 146163.0 57 132685.0 58 119785.0 59 109232.5 60 96248.0 61 80804.0 62 67057.0 63 54828.5 64 44006.0 65 34833.0 66 26588.0 67 20092.0 68 15277.0 69 11650.0 70 8743.0 71 6402.0 72 4767.0 73 3474.0 74 2564.0 75 1790.5 76 1064.0 77 642.0 78 425.0 79 281.5 80 155.0 81 69.5 82 47.5 83 24.0 84 15.5 85 10.5 86 5.5 87 2.5 88 1.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05921696833153077 2 1.1867127922150454E-4 3 0.0 4 0.11841910275315884 5 0.0015575605397822473 6 0.0012312145219231098 7 0.0 8 0.0 9 1.4833909902688067E-5 10-11 0.0 12-13 1.4833909902688067E-5 14-15 7.4169549513440335E-6 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.6317300892956875E-4 30-31 2.5217646834569714E-4 32-33 7.4169549513440335E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6317300892956875E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.780069188322568E-4 80-81 3.040951530051054E-4 82-83 3.115121079564494E-4 84-85 0.0 86-87 0.0 88-89 5.933563961075227E-5 90-91 5.933563961075227E-5 92-93 7.491124500857474E-4 94-95 7.120276753290272E-4 96-97 0.0 98-99 2.9667819805376134E-5 100 0.0011570449724096691 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6741311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.877375935449926 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04594873264918 46.903551853008864 2 14.110376876345823 17.180054353843683 3 4.027331673169973 7.355201528529391 4 1.630282005392499 3.9698916209231343 5 0.8829270637583742 2.687514139200075 6 0.5344618249882761 1.9521980065774758 7 0.330916885942396 1.4101746168232554 8 0.2510071565478618 1.2224525625322014 9 0.18647781852934786 1.0217052236010347 >10 0.9273546625609159 9.989319360910224 >50 0.05051606353474456 2.1022553607503007 >100 0.02088956670940866 2.2676533627938453 >500 9.009595075447106E-4 0.38788740694912 >1k 4.869682924064946E-4 0.4926612760570874 >5k 4.869682846918597E-5 0.17904309939273047 >10k+ 7.304524270377895E-5 0.8784362281075739 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23121 0.3429748308600508 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22286 0.33058851609130624 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13856 0.20553865561164586 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.4833909902688067E-5 0.0 4 0.0 0.0 0.0 1.4833909902688067E-5 0.0 5 0.0 2.9667819805376134E-5 0.0 1.4833909902688067E-5 0.0 6 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 0.0 7 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 0.0 8 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 0.0 9 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 0.0 10-11 0.0 2.9667819805376134E-5 0.0 2.9667819805376134E-5 0.0 12-13 0.0 2.9667819805376134E-5 0.0 4.45017297080642E-5 0.0 14-15 0.0 2.9667819805376134E-5 0.0 5.1918684659408235E-5 0.0 16-17 0.0 2.9667819805376134E-5 0.0 6.675259456209631E-5 0.0 18-19 0.0 5.933563961075227E-5 0.0 7.416954951344034E-5 0.0 20-21 0.0 7.416954951344034E-5 0.0 8.158650446478437E-5 0.0 22-23 0.0 7.416954951344034E-5 0.0 2.5959342329704116E-4 0.0 24-25 0.0 9.642041436747244E-5 0.0 6.67525945620963E-4 0.0 26-27 0.0 1.1867127922150454E-4 0.0 0.0011496280174583251 0.0 28-29 0.0 1.1867127922150454E-4 0.0 0.0031373719444185264 0.0 30-31 0.0 1.1867127922150454E-4 0.0 0.008818759437148056 0.0 32-33 0.0 1.1867127922150454E-4 0.0 0.02124957593560066 0.0 34-35 0.0 1.1867127922150454E-4 0.0 0.04192062938499648 0.0 36-37 0.0 1.7058996388091276E-4 0.0 0.07320534536976561 0.0 38-39 0.0 2.15091693588977E-4 0.0 0.116676118339593 0.0 40-41 0.0 2.3734255844300907E-4 0.0 0.16578377707244185 0.0 42-43 0.0 2.6701037824838523E-4 0.0 0.2187482523799896 0.0 44-45 0.0 2.7442733319972925E-4 0.0 0.2830384178982397 0.0 46-47 0.0 2.8184428815107327E-4 0.0 0.3630970296430472 0.0 48-49 0.0 2.8184428815107327E-4 0.0 0.4488741136553409 0.0 50-51 0.0 3.115121079564494E-4 0.0 0.5404735073044398 0.0 52-53 0.0 3.115121079564494E-4 0.0 0.6347578386459252 0.0 54-55 0.0 3.115121079564494E-4 0.0 0.743238221764283 0.0 56-57 0.0 3.115121079564494E-4 0.0 0.8719965597196153 0.0 58-59 0.0 3.115121079564494E-4 0.0 1.017227954621883 0.0 60-61 0.0 3.115121079564494E-4 0.0 1.1717527940781844 0.0 62-63 0.0 3.115121079564494E-4 0.0 1.3254914363096437 0.0 64-65 0.0 3.115121079564494E-4 0.0 1.499953050675158 0.0 66-67 0.0 3.263460178591375E-4 0.0 1.7095636145550916 0.0 68-69 0.0 3.263460178591375E-4 0.0 1.9418774775410896 0.0 70-71 0.0 3.263460178591375E-4 0.0 2.1938240202832953 0.0 72-73 0.0 3.560138376645136E-4 0.0 2.4670646406908094 0.0 74-75 0.0 3.560138376645136E-4 0.0 2.7445477593304926 0.0 76-77 0.0 3.560138376645136E-4 0.0 3.0516616130007947 0.0 78-79 0.0 3.634307926158577E-4 0.0 3.4023871617849997 0.0 80-81 0.0 3.708477475672017E-4 0.0 3.7838189040677697 0.0 82-83 0.0 3.8568165746988975E-4 0.0 4.182680490486198 0.0 84-85 0.0 3.8568165746988975E-4 0.0 4.593349869187166 0.0 86-87 0.0 3.8568165746988975E-4 0.0 5.026106939733236 0.0 88 0.0 4.45017297080642E-4 0.0 5.384353280838104 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12780 0.0 58.246456 1 GTATCAA 22670 0.0 38.588924 1 GTGGTAT 5080 0.0 33.458927 1 ATCAACG 28290 0.0 30.638243 3 TCAACGC 28575 0.0 30.398535 4 TGGTATC 5450 0.0 30.046227 2 TATCAAC 29005 0.0 29.91543 2 CAACGCA 29610 0.0 29.364866 5 AACGCAG 31430 0.0 27.887724 6 ACGCAGA 36085 0.0 24.22452 7 CGCAGAG 36045 0.0 24.02975 8 GTACATG 17050 0.0 22.837559 1 GCAGAGT 38585 0.0 22.15558 9 TACATGG 17475 0.0 21.487846 2 GAGTACT 29560 0.0 20.77974 12-13 CAGAGTA 37665 0.0 20.550604 10-11 ACATGGG 17660 0.0 20.330172 3 AGAGTAC 35965 0.0 19.92777 10-11 ATAACGC 580 0.0 19.4711 3 AGTACTT 31070 0.0 18.726143 12-13 >>END_MODULE