FastQCFastQC Report
Wed 25 May 2016
SRR2049493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361354
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.044.4138451
GTATCAA82550.035.9795761
ATCAACG91950.030.9582583
TCAACGC91750.030.8025364
CAACGCA99250.028.6296425
TATCAAC103250.027.9171942
AACGCAG109900.026.0415956
GTACATG140500.024.8572581
TACCTGG40750.023.3689292
TACATGG140950.023.3197252
GTACCTG45450.022.6468261
ACGCAGA124450.022.6262257
GTGGTAT33450.022.3512551
CGCAGAG126900.022.1017448
TATAACG5350.022.0103822
ACATGGG148150.021.0459793
TCTGTCG17150.020.046848
TGGTATC35500.019.4696832
CATGGGG100800.018.894524
ATAACGC5550.018.449753
Produced by FastQC (version 0.11.5)