##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049493_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5361354 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.669852988629366 31.0 31.0 34.0 27.0 34.0 2 30.893986854813168 31.0 31.0 34.0 27.0 34.0 3 30.945599190055347 31.0 31.0 34.0 27.0 34.0 4 34.522891978406946 37.0 35.0 37.0 32.0 37.0 5 34.551754650038035 37.0 35.0 37.0 32.0 37.0 6 34.49867645374657 37.0 35.0 37.0 31.0 37.0 7 30.58670589556295 37.0 33.0 37.0 2.0 37.0 8 32.32799568914867 37.0 33.0 37.0 17.0 37.0 9 35.07270029175466 39.0 34.0 39.0 28.0 39.0 10-11 35.62626959532984 39.0 36.0 39.0 30.0 39.0 12-13 35.791736285274204 39.0 37.0 39.0 30.0 39.0 14-15 36.963948845758 40.0 37.0 41.0 30.5 41.0 16-17 36.96834484348543 40.0 37.0 41.0 30.5 41.0 18-19 36.95646659407306 40.0 37.0 41.0 30.0 41.0 20-21 37.03653237596323 40.0 37.0 41.0 30.0 41.0 22-23 36.99301482424029 40.0 37.0 41.0 30.0 41.0 24-25 36.967266664353815 40.0 37.0 41.0 30.0 41.0 26-27 36.99924086340876 40.0 37.0 41.0 30.0 41.0 28-29 36.98067447141152 40.0 37.0 41.0 30.0 41.0 30-31 36.89875113637339 40.0 37.0 41.0 30.0 41.0 32-33 36.84914314555614 40.0 37.0 41.0 30.0 41.0 34-35 36.78824295131416 40.0 37.0 41.0 30.0 41.0 36-37 36.60434864028751 40.0 36.5 41.0 30.0 41.0 38-39 36.48822899961465 40.0 36.0 41.0 30.0 41.0 40-41 36.40324431104531 40.0 36.0 41.0 30.0 41.0 42-43 36.27457317685048 40.0 36.0 41.0 29.5 41.0 44-45 36.1385967984953 40.0 35.5 41.0 29.0 41.0 46-47 35.975591054050895 40.0 35.0 41.0 28.5 41.0 48-49 35.883397552185514 39.0 35.0 41.0 28.0 41.0 50-51 35.73989984619557 39.0 35.0 41.0 28.0 41.0 52-53 35.61064667619411 39.0 35.0 41.0 28.0 41.0 54-55 35.47837281403168 39.0 35.0 41.0 28.0 41.0 56-57 35.275284564309686 39.0 35.0 41.0 27.0 41.0 58-59 35.09000646105443 39.0 34.0 40.0 27.0 41.0 60-61 34.85602741397042 38.0 34.0 40.0 26.5 41.0 62-63 34.60091415340229 38.0 34.0 40.0 26.0 41.0 64-65 34.227720180387266 37.5 34.0 40.0 25.5 41.0 66-67 30.770592745787724 36.0 30.0 39.5 6.5 41.0 68-69 31.107767086448685 35.5 29.5 39.0 11.0 41.0 70-71 31.25385229179047 35.0 29.0 39.0 14.5 41.0 72-73 32.461521940166605 35.0 31.5 39.0 21.5 40.0 74-75 32.55026799200351 35.0 32.5 38.0 22.5 40.0 76-77 32.25825491097957 35.0 32.5 37.0 22.0 39.5 78-79 28.976508639422057 35.0 29.0 36.5 6.0 39.0 80-81 30.6979306906427 35.0 29.5 36.0 16.0 39.0 82-83 31.09215004269444 35.0 31.5 36.0 18.5 37.5 84-85 29.281296385204186 34.5 29.5 35.5 10.0 37.0 86-87 28.911079831699233 34.0 27.0 35.0 10.0 37.0 88-89 30.133108352852656 34.0 30.5 35.0 9.0 36.0 90-91 30.28353779660884 35.0 31.0 35.0 4.5 36.0 92-93 30.131306569198752 35.0 31.0 35.0 2.0 36.0 94-95 29.920949166945512 35.0 31.0 35.0 2.0 36.0 96-97 29.722145655742935 34.0 31.0 35.0 2.0 35.0 98-99 29.527192757650397 34.0 31.0 35.0 2.0 35.0 100 29.31203778000856 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3849719364529882 1101 2 0.42862478347794664 1101 3 0.45819667166800215 1101 4 0.45183013647160664 1101 5 0.39679797332696154 1101 6 0.46509290472778275 1101 7 -12.085112536245003 1101 8 -6.449082973095912 1101 9 -2.097743042095317 1101 10-11 0.012290425032567498 1101 12-13 0.5619187614395145 1101 14-15 0.7183214036380292 1101 16-17 0.7639838338520164 1101 18-19 0.7522411118075141 1101 20-21 0.7339928936868176 1101 22-23 0.7009943519964352 1101 24-25 0.7537850097241758 1101 26-27 0.7312331327811421 1101 28-29 0.6495486672518638 1101 30-31 0.7076262454907507 1101 32-33 0.7437548027196854 1101 34-35 0.7212426216893846 1101 36-37 0.7712931602741051 1101 38-39 0.782827756810093 1101 40-41 0.6920210239777518 1101 42-43 0.7105707538697104 1101 44-45 0.6351233512669694 1101 46-47 0.6519889236353364 1101 48-49 0.6057809031930645 1101 50-51 0.7042867825805672 1101 52-53 0.6614122729158041 1101 54-55 0.664809522609076 1101 56-57 0.5606430070173403 1101 58-59 0.5398760279883348 1101 60-61 0.5904408846381486 1101 62-63 0.6526109975426024 1101 64-65 0.4987354924478993 1101 66-67 -10.122294501971009 1101 68-69 -8.002074714571062 1101 70-71 -6.529585811908792 1101 72-73 -1.4027785750836728 1101 74-75 -0.14971726304641209 1101 76-77 0.13596134041981145 1101 78-79 3.217593389822458 1101 80-81 1.1582764746504353 1101 82-83 0.4964398648964057 1101 84-85 -5.21727666427358 1101 86-87 -5.7529724417486285 1101 88-89 -1.2039025561086945 1101 90-91 0.14170821574904124 1101 92-93 0.4737368269547808 1101 94-95 0.5599293394055849 1101 96-97 0.6478632147745671 1101 98-99 0.6559404289663249 1101 100 0.6998232062879097 1102 1 0.320010850828492 1102 2 0.3345475420213546 1102 3 0.356411313436773 1102 4 0.3408276449820775 1102 5 0.3246941012583733 1102 6 0.3781514023222101 1102 7 -12.113499627897102 1102 8 -6.525163078766937 1102 9 -2.210071702569863 1102 10-11 -0.07951778319030467 1102 12-13 0.5100743694873628 1102 14-15 0.6783000190844533 1102 16-17 0.6638262048286734 1102 18-19 0.6763550542685479 1102 20-21 0.6082585789366703 1102 22-23 0.6235619600164526 1102 24-25 0.6555327999292615 1102 26-27 0.6504941581827737 1102 28-29 0.6171722090142069 1102 30-31 0.6250174121448779 1102 32-33 0.6822964026066174 1102 34-35 0.6905629413731234 1102 36-37 0.728887850743682 1102 38-39 0.6682004133376793 1102 40-41 0.6677763567470905 1102 42-43 0.6185942038865448 1102 44-45 0.5957084709570637 1102 46-47 0.6587127661692236 1102 48-49 0.5849186086591089 1102 50-51 0.5886146237388346 1102 52-53 0.5988377615982969 1102 54-55 0.5513012019811612 1102 56-57 0.6127424866189486 1102 58-59 0.5528964189469292 1102 60-61 0.5050793448047983 1102 62-63 0.41010330320584387 1102 64-65 0.29112832476999273 1102 66-67 -10.148321941057137 1102 68-69 -6.580389543338821 1102 70-71 -6.110653548268299 1102 72-73 -0.9984041795112972 1102 74-75 -0.03534939654271341 1102 76-77 0.04728551480022958 1102 78-79 3.1059413490320686 1102 80-81 1.0390928079404347 1102 82-83 0.41590637514242346 1102 84-85 -5.3673004662753385 1102 86-87 -5.851859598925742 1102 88-89 -1.2872319635369536 1102 90-91 1.0367893466423084E-4 1102 92-93 0.29001896652092896 1102 94-95 0.31250208380349065 1102 96-97 0.4779517569620282 1102 98-99 0.501683854264467 1102 100 0.6130523277389024 1103 1 0.2824698180379741 1103 2 0.3133537002260667 1103 3 0.2921670253180082 1103 4 0.30638032071193066 1103 5 0.30859185972689573 1103 6 0.33636852263737893 1103 7 -12.148785042883993 1103 8 -6.593572891760097 1103 9 -2.2702486110034883 1103 10-11 -0.2579251293045459 1103 12-13 0.3190459317879686 1103 14-15 0.47823520275250075 1103 16-17 0.4962312433242033 1103 18-19 0.5311398204338715 1103 20-21 0.48126045934111517 1103 22-23 0.5246630022019048 1103 24-25 0.615576138076861 1103 26-27 0.5738037267091798 1103 28-29 0.5479906018311169 1103 30-31 0.5854159464245399 1103 32-33 0.6040657330231696 1103 34-35 0.6064181591426987 1103 36-37 0.6336601474909926 1103 38-39 0.6483964778894276 1103 40-41 0.6394537479909062 1103 42-43 0.6233678238639158 1103 44-45 0.6122615558755626 1103 46-47 0.6497468107184616 1103 48-49 0.6240760074831542 1103 50-51 0.6440149585565536 1103 52-53 0.5188211955680018 1103 54-55 0.5342558889802049 1103 56-57 0.5991811253014632 1103 58-59 0.5194925054669994 1103 60-61 0.47107729420326194 1103 62-63 0.4101714816223563 1103 64-65 0.2619754482197294 1103 66-67 -10.226901831983824 1103 68-69 -8.248829245466233 1103 70-71 -6.7252868170806686 1103 72-73 -1.5877384877816354 1103 74-75 -0.35503713922631164 1103 76-77 -0.03092050455984463 1103 78-79 3.0551228670996657 1103 80-81 1.0578643623286688 1103 82-83 0.36740011960604235 1103 84-85 -5.443844118791343 1103 86-87 -5.9765069935566295 1103 88-89 -1.4791706873180104 1103 90-91 -0.1597098796367291 1103 92-93 0.25058519151905756 1103 94-95 0.33209953460076846 1103 96-97 0.43563475816124253 1103 98-99 0.41855295487781774 1103 100 0.5337357065312638 1104 1 0.3123493768437413 1104 2 0.38628076637728626 1104 3 0.34193415947289196 1104 4 0.4307588951237378 1104 5 0.39340756662140564 1104 6 0.4706231236715226 1104 7 -11.986823345422025 1104 8 -6.4605059026229945 1104 9 -2.1645159346806224 1104 10-11 -0.15932823308683197 1104 12-13 0.436683390201992 1104 14-15 0.6213624581390107 1104 16-17 0.6457172115037721 1104 18-19 0.7193744148053938 1104 20-21 0.687899255497868 1104 22-23 0.6867665804811125 1104 24-25 0.7779835650012288 1104 26-27 0.6937220390976719 1104 28-29 0.6852607327332123 1104 30-31 0.6473092110933365 1104 32-33 0.6165856472072946 1104 34-35 0.5966547958675434 1104 36-37 0.6664091488143811 1104 38-39 0.6372565674253252 1104 40-41 0.6704732120433832 1104 42-43 0.5740137777790935 1104 44-45 0.5469159992918833 1104 46-47 0.5753967950792926 1104 48-49 0.5209815774025159 1104 50-51 0.5325376254437373 1104 52-53 0.5294626807754881 1104 54-55 0.565282103050869 1104 56-57 0.5802694004089801 1104 58-59 0.5235532446742042 1104 60-61 0.45039253192259565 1104 62-63 0.36779470434582606 1104 64-65 0.2807917272027396 1104 66-67 -10.158284407884679 1104 68-69 -8.167577352526344 1104 70-71 -6.586495225555506 1104 72-73 -1.5640021629271956 1104 74-75 -0.31808861571289526 1104 76-77 -0.032582085125845595 1104 78-79 3.056784121577831 1104 80-81 1.0383253422669654 1104 82-83 0.3990382701088784 1104 84-85 -5.312919689777665 1104 86-87 -5.834893829208099 1104 88-89 -1.320320096068592 1104 90-91 -0.01194340522275894 1104 92-93 0.39323472822650274 1104 94-95 0.3872019548768577 1104 96-97 0.513038488553903 1104 98-99 0.5508780941890628 1104 100 0.5896223354430461 1105 1 0.14269879676782793 1105 2 0.13667126096087046 1105 3 0.1387227704329348 1105 4 0.1980704797777335 1105 5 0.19757386472591776 1105 6 0.18707816572925395 1105 7 -12.178187540922686 1105 8 -6.621481125238166 1105 9 -2.44175880608762 1105 10-11 -0.3676302088514305 1105 12-13 0.12944521069744042 1105 14-15 0.3122980155924111 1105 16-17 0.39182556032396576 1105 18-19 0.3363615601240113 1105 20-21 0.37677034945644294 1105 22-23 0.3573902190415126 1105 24-25 0.3809814842904302 1105 26-27 0.3057515520218814 1105 28-29 0.3040514756867623 1105 30-31 0.3534988672635393 1105 32-33 0.3731578071812649 1105 34-35 0.4334858850795271 1105 36-37 0.500867542088919 1105 38-39 0.48586153143076416 1105 40-41 0.429300855864831 1105 42-43 0.37514452751190674 1105 44-45 0.3126497623598823 1105 46-47 0.27579914995681776 1105 48-49 0.278823715025311 1105 50-51 0.35915688812755775 1105 52-53 0.32049954646033285 1105 54-55 0.34702420928864797 1105 56-57 0.3305077640340386 1105 58-59 0.3372193631648841 1105 60-61 0.28020740721763104 1105 62-63 0.25569537699480804 1105 64-65 0.0650242931456404 1105 66-67 -10.18703729890903 1105 68-69 -8.289597904339576 1105 70-71 -6.691433970369658 1105 72-73 -1.6601514384640161 1105 74-75 -0.5114128289070266 1105 76-77 -0.23555369638096835 1105 78-79 2.8376884003031932 1105 80-81 0.7796744994992153 1105 82-83 0.1348153813701849 1105 84-85 -5.478719217491811 1105 86-87 -5.9967426451460994 1105 88-89 -1.611022077837923 1105 90-91 -0.3243543947423717 1105 92-93 0.028086254813548095 1105 94-95 0.055006776707955396 1105 96-97 0.14815968453401496 1105 98-99 0.12066158042052066 1105 100 0.22444756798219956 1106 1 0.029867932189766577 1106 2 0.07217597670918963 1106 3 0.10171837206029721 1106 4 0.04597677705987735 1106 5 0.13789685231850513 1106 6 0.13964415159117038 1106 7 -12.187047092328758 1106 8 -6.714953756664045 1106 9 -2.6171691156419143 1106 10-11 -0.48718639554715537 1106 12-13 0.01848649255848045 1106 14-15 0.21288481238713786 1106 16-17 0.24066168210479333 1106 18-19 0.2539435129696628 1106 20-21 0.27741113371608606 1106 22-23 0.2771170221468182 1106 24-25 0.31013485181252065 1106 26-27 0.31618792456443856 1106 28-29 0.2503719447741233 1106 30-31 0.22133856927587203 1106 32-33 0.25099053777201163 1106 34-35 0.316246731497948 1106 36-37 0.38637787018986813 1106 38-39 0.259777329409026 1106 40-41 0.32382344891799875 1106 42-43 0.2881078709058471 1106 44-45 0.1851237753575532 1106 46-47 0.3030352808633694 1106 48-49 0.20269290459195588 1106 50-51 0.23047970502808113 1106 52-53 0.20508320965318916 1106 54-55 0.18809038599428618 1106 56-57 0.23813699904063412 1106 58-59 0.18034849294821242 1106 60-61 0.1728215111228053 1106 62-63 0.10134803323074237 1106 64-65 -0.025800912857818048 1106 66-67 -10.312715266580842 1106 68-69 -8.314519657374788 1106 70-71 -6.846236169094649 1106 72-73 -1.8841569191225247 1106 74-75 -0.6150559451670432 1106 76-77 -0.28820068643256747 1106 78-79 2.782737749770927 1106 80-81 0.7317401130115364 1106 82-83 0.10020901585127007 1106 84-85 -5.5873678521325765 1106 86-87 -6.0666186009537775 1106 88-89 -1.6297452555280927 1106 90-91 -0.40228280456032195 1106 92-93 0.0075592920613978265 1106 94-95 0.06093815897381205 1106 96-97 0.15021814298280844 1106 98-99 0.20527624023563362 1106 100 0.32556761021924885 1107 1 0.11291498950653178 1107 2 0.17346356476347324 1107 3 0.08681619011804642 1107 4 0.07252619606486377 1107 5 0.12572192760922718 1107 6 0.12008608578502589 1107 7 -12.168115913291228 1107 8 -6.677250265264345 1107 9 -2.564549084341273 1107 10-11 -0.537573009353693 1107 12-13 0.13018411603902535 1107 14-15 0.17988619467093514 1107 16-17 0.1836550331068878 1107 18-19 0.2086279080520228 1107 20-21 0.2312690678558198 1107 22-23 0.28773964682289943 1107 24-25 0.3395403068902709 1107 26-27 0.2424852416237897 1107 28-29 0.20934393990233957 1107 30-31 0.2836056317208602 1107 32-33 0.31726148903163676 1107 34-35 0.2219621252824595 1107 36-37 0.34699741567177256 1107 38-39 0.3197417687086741 1107 40-41 0.32812827415899193 1107 42-43 0.27870365476459114 1107 44-45 0.2685680356568554 1107 46-47 0.2669250583132694 1107 48-49 0.20035527938554765 1107 50-51 0.2502988200093128 1107 52-53 0.2109419246103741 1107 54-55 0.2116884097236209 1107 56-57 0.15806891328114148 1107 58-59 0.17001608341185204 1107 60-61 0.1592013105173038 1107 62-63 0.08613388870477934 1107 64-65 -0.06942947940596866 1107 66-67 -10.285476854288266 1107 68-69 -8.35489427350264 1107 70-71 -6.839826632611075 1107 72-73 -1.8451711632918517 1107 74-75 -0.5980088308249378 1107 76-77 -0.33610322820936744 1107 78-79 2.7489177813259182 1107 80-81 0.7949352744868889 1107 82-83 0.13942648068058716 1107 84-85 -5.496355670050665 1107 86-87 -5.997129296558697 1107 88-89 -1.575222598712834 1107 90-91 -0.3435941403008158 1107 92-93 0.023279100954500365 1107 94-95 0.08370440104074817 1107 96-97 0.10933749228767198 1107 98-99 0.13926336929109695 1107 100 0.20269732448948474 1108 1 -0.10039532614556634 1108 2 -0.09040972438254258 1108 3 -0.06666050251759614 1108 4 -0.09445954014385904 1108 5 -0.15285532123456136 1108 6 -0.0456814601406208 1108 7 -12.317745192051174 1108 8 -6.859292441235759 1108 9 -2.7737146876409895 1108 10-11 -0.7179293214495672 1108 12-13 -0.16646799973608495 1108 14-15 0.0033730582992959057 1108 16-17 -0.023733250718890986 1108 18-19 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2216 20-21 -1.6018260961211581 2216 22-23 -1.4575242584610848 2216 24-25 -1.4810543116918495 2216 26-27 -1.8763677502791225 2216 28-29 -2.0312570841451887 2216 30-31 -1.9138083479489936 2216 32-33 -2.848173446640061 2216 34-35 -1.9250548259706122 2216 36-37 -1.593631661528839 2216 38-39 -1.847116117113437 2216 40-41 -1.9064631785759687 2216 42-43 -1.563201553922255 2216 44-45 -1.6489493019153088 2216 46-47 -1.859024690391628 2216 48-49 -1.9138797957549087 2216 50-51 -2.4372727273637693 2216 52-53 -2.121454644314646 2216 54-55 -1.813748412116638 2216 56-57 -1.90160061660427 2216 58-59 -2.1204651904216405 2216 60-61 -2.291487527271009 2216 62-63 -2.2704722820205134 2216 64-65 -1.8876164993020836 2216 66-67 1.4403819834858886 2216 68-69 0.6288132451340864 2216 70-71 0.2535963242853789 2216 72-73 -1.477856130490359 2216 74-75 -2.3028086265725207 2216 76-77 -1.9710154193649068 2216 78-79 0.5682017929382326 2216 80-81 -0.9070242925223653 2216 82-83 -1.739846191614955 2216 84-85 0.01529018311500252 2216 86-87 0.0470462181988438 2216 88-89 -1.219532528141734 2216 90-91 -1.4989698866213175 2216 92-93 -1.5901072799401312 2216 94-95 -1.5844362779734027 2216 96-97 -1.6010077315059306 2216 98-99 -1.6145413106918731 2216 100 -2.7633507710134246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95153.0 3 17247.0 4 10078.0 5 9248.0 6 8374.0 7 9031.0 8 9869.0 9 10966.0 10 12436.0 11 14076.0 12 16035.0 13 18057.0 14 20786.0 15 22369.0 16 23813.0 17 24621.0 18 22996.0 19 21130.0 20 20907.0 21 21267.0 22 22551.0 23 26422.0 24 31524.0 25 39426.0 26 49374.0 27 60146.0 28 72692.0 29 92946.0 30 113256.0 31 137781.0 32 182659.0 33 255739.0 34 385462.0 35 518141.0 36 587988.0 37 977057.0 38 1061683.0 39 337387.0 40 661.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15195339062496 20.038561310524887 13.805919263771862 23.003566035078293 2 16.133199558535708 20.535774406299726 39.655199749162925 23.675826286001637 3 18.344831805467788 25.42735967375028 30.318550080838726 25.909258439943205 4 13.069782103460753 17.512133341789056 38.28232542350119 31.135759131248996 5 13.062611459111096 36.94752606238334 34.79950929546371 15.190353183041857 6 30.002169294908278 37.76734974275296 18.27312933754061 13.957351624798155 7 26.75655792814539 32.399188973244776 22.47749464082724 18.366758457782595 8 24.668754323768646 35.520884373776546 21.201476567575387 18.608884734879425 9 24.38675006351008 17.92248749103305 22.12230343305068 35.568459012406194 10-11 23.45532490486545 27.13644724821379 28.83635365245421 20.571874194466545 12-13 24.172671679579448 24.75596649652308 28.738253433740805 22.333108390156667 14-15 21.798523283483988 27.215904788230734 26.06116850332957 24.92440342495571 16-17 21.224153487819038 30.24912160445038 26.53737989820637 21.989345009524207 18-19 21.106207499075794 29.050338776361347 28.564127643875036 21.279326080687827 20-21 22.393158519284494 28.556256497892136 27.897980622059276 21.15260436076409 22-23 22.058877291072367 28.623571582850154 27.87199837951383 21.44555274656365 24-25 21.79013920737187 28.45109649540023 28.07280586210125 21.685958435126647 26-27 21.55059842282055 28.363341050926977 28.110288783985656 21.97577174226682 28-29 21.637155464832205 28.438319872181545 28.15010909557548 21.77441556741077 30-31 22.03365978071957 28.982128395177785 27.5935519273676 21.39065989673504 32-33 21.482371803839104 28.616577081088096 28.214150753708857 21.68690036136394 34-35 21.57153113086608 28.163386430024822 28.23042177242266 22.034660666686438 36-37 22.189434067348852 28.572710230728116 27.661848822316976 21.576006879606055 38-39 21.947264602964385 28.610481890620303 27.86690072456544 21.575352781849872 40-41 21.66030260266343 28.34642144503049 28.075501076780228 21.917774875525847 42-43 21.555852101844096 28.393754820962585 28.04504610451301 22.005346972680304 44-45 21.92186120477802 28.579323712550085 27.85014094878626 21.64867413388563 46-47 21.729113814970866 28.904472360937362 27.636386052878525 21.73002777121325 48-49 21.634806658585322 28.43924228347539 28.00585079902912 21.920100258910168 50-51 21.653189762060084 28.26334684521059 27.98059064607539 22.102872746653933 52-53 21.65389016909294 28.36974663897086 27.88565138034949 22.090711811586708 54-55 21.819628710048082 28.824292007094126 27.671817964012885 21.684261318844904 56-57 21.48746534056408 28.59725798408569 27.70634109482736 22.20893558052288 58-59 21.4223589543869 28.169179194800993 28.063543152421232 22.344918698390867 60-61 21.862738743460625 28.598005417557122 27.590166327117306 21.94908951186495 62-63 21.658186183528915 28.61956250163792 27.637409427292965 22.084841887540204 64-65 21.64661257460073 28.28412706154851 27.91781607099707 22.15144429285369 66-67 21.51041157619251 28.356989772460693 27.697982248684188 22.43461640266261 68-69 22.138662008604037 28.61018844653276 27.421837904432174 21.82931164043103 70-71 21.911713068638857 28.226944930834208 27.687290888022066 22.174051112504866 72-73 21.603121636770258 28.352327518190584 28.092390077761138 21.952160767278027 74-75 21.548127163991186 28.389997551848356 27.89298079949638 22.16889448466408 76-77 21.647848639773805 28.439713686070338 27.629656082492225 22.282781591663632 78-79 21.790613719834642 28.49918933589403 27.796899569498528 21.913297374772796 80-81 21.53244411217408 28.290595803544647 27.9511689400824 22.225791144198872 82-83 21.427538192463548 28.28594896554413 28.082128987063946 22.204383854928373 84-85 21.640436551660557 28.262742205357682 27.822389982994515 22.274431259987242 86-87 21.75155069110069 28.35162664484054 27.79588850020835 22.10093416385042 88-89 21.43562534139599 28.232796789634975 27.94499128423088 22.386586584738158 90-91 21.335353538368103 28.152612898821378 27.90623020488804 22.605803357922486 92-93 21.609598686848905 28.324930285479795 27.71237514339274 22.35309588427856 94-95 21.545524340754902 28.12361269970596 27.899695437456117 22.431167522083022 96-97 21.454611077666023 28.1147151496612 27.99643922504182 22.43423454763096 98-99 21.460075059782955 28.09553521132015 27.968004461804195 22.476385267092702 100 21.670396050906653 28.122945823077217 27.73546416843934 22.471193957576794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2690.0 1 2192.0 2 2012.0 3 2246.0 4 2052.5 5 1808.0 6 1654.5 7 1628.5 8 1560.0 9 1541.0 10 1490.5 11 1355.0 12 1333.5 13 1444.5 14 1580.5 15 1728.0 16 1985.0 17 2313.0 18 2906.0 19 3865.0 20 5311.5 21 7479.5 22 11066.0 23 16559.5 24 22870.0 25 30342.0 26 40820.0 27 54107.5 28 70522.5 29 87331.0 30 100894.5 31 114337.0 32 127488.5 33 138934.0 34 150197.0 35 160208.0 36 167793.5 37 174124.5 38 179473.5 39 182420.0 40 183674.0 41 182547.0 42 178863.5 43 176120.5 44 173549.0 45 171201.0 46 170517.5 47 168783.5 48 167012.5 49 166977.5 50 164623.0 51 158508.0 52 151132.5 53 145157.0 54 140237.5 55 133157.5 56 123719.5 57 112739.0 58 101707.0 59 91785.0 60 80894.5 61 68471.5 62 57023.5 63 47037.5 64 38073.5 65 30092.5 66 23458.0 67 18188.0 68 13824.0 69 10547.0 70 7983.5 71 5983.5 72 4654.0 73 3466.0 74 2486.0 75 1818.0 76 1283.5 77 894.0 78 651.5 79 505.5 80 378.5 81 269.0 82 185.0 83 145.0 84 125.0 85 96.0 86 81.0 87 70.5 88 54.0 89 45.5 90 36.5 91 23.5 92 20.0 93 21.5 94 17.5 95 13.5 96 12.5 97 11.0 98 14.0 99 17.0 100 28.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019752472976042993 2 0.0019584604933753674 3 0.0011564242913264075 4 0.001753288441688424 5 0.001548116390001481 6 0.0030775807753041485 7 11.64163008075945 8 1.865200469881302E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.077580775304149E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.051720516869433E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 5.595601409643907E-5 36-37 9.32600234940651E-6 38-39 4.6630011747032565E-5 40-41 0.0 42-43 4.6630011747032565E-5 44-45 1.7719404463872372E-4 46-47 8.76644220844212E-4 48-49 6.528201644584559E-4 50-51 4.0101810102447996E-4 52-53 2.331500587351628E-4 54-55 3.450620869280409E-4 56-57 0.0014828343735556354 58-59 3.916920986750735E-4 60-61 0.0015201383829532614 62-63 0.0022848705756045953 64-65 0.001165750293675814 66-67 5.821188080473702 68-69 5.467881807468785 70-71 0.002639258664882043 72-73 0.0011937283007240334 74-75 0.0031055587823523682 76-77 0.005884707482475509 78-79 5.366853224017664 80-81 0.0048122172122937595 82-83 0.004672327177052662 84-85 5.821299992501894 86-87 0.0036557929209673524 88-89 0.0017626144440378308 90-91 0.0033946648551839704 92-93 0.0038050089585578567 94-95 0.004140745043136491 96-97 0.003012298758858303 98-99 0.005483689381451029 100 0.006080553531813046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5361354.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.09899749091997 #Duplication Level Percentage of deduplicated Percentage of total 1 92.52832658423452 82.44181138167735 2 5.387118488006208 9.599737132923075 3 1.157602362987525 3.0942363010592557 4 0.42702363942837934 1.5218951271197074 5 0.19397683985009442 0.8641570983550074 6 0.10047984724943504 0.5371592194779261 7 0.06361746067691283 0.39677763784616693 8 0.0440984905220903 0.3143305037104866 9 0.024686409275870118 0.19795808863175174 >10 0.07180817471763504 0.9416568051789158 >50 9.957179051149512E-4 0.05604277368541999 >100 2.6598514618516027E-4 0.03423793033492891 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.8652004698813025E-5 0.0 0.0 0.0 6 0.0 1.8652004698813025E-5 0.0 0.0 0.0 7 0.0 1.8652004698813025E-5 0.0 0.0 0.0 8 0.0 1.8652004698813025E-5 0.0 0.0 0.0 9 0.0 1.8652004698813025E-5 0.0 0.0 0.0 10-11 0.0 1.8652004698813025E-5 0.0 9.326002349406512E-6 0.0 12-13 0.0 3.730400939762605E-5 0.0 4.6630011747032565E-5 0.0 14-15 0.0 7.46080187952521E-5 0.0 5.5956014096439074E-5 0.0 16-17 0.0 1.2123803054228465E-4 0.0 7.46080187952521E-5 0.0 18-19 0.0 1.5854203993991071E-4 0.0 1.5854203993991071E-4 0.0 20-21 0.0 1.678680422893172E-4 0.0 2.424760610845693E-4 0.0 22-23 0.0 1.8652004698813023E-4 0.0 4.756261198197321E-4 0.0 24-25 0.0 1.8652004698813023E-4 0.0 9.419262372900577E-4 0.0 26-27 0.0 1.8652004698813023E-4 0.0 0.001753288441688424 0.0 28-29 1.8652004698813025E-5 1.8652004698813023E-4 0.0 0.0043365910924740275 0.0 30-31 1.8652004698813025E-5 1.8652004698813023E-4 0.0 0.010967378762902057 0.0 32-33 2.7978007048219537E-5 1.8652004698813023E-4 0.0 0.025338748383337495 0.0 34-35 3.730400939762605E-5 1.9584604933753675E-4 0.0 0.0485604942333597 0.0 36-37 3.730400939762605E-5 2.424760610845693E-4 0.0 0.0840645851775503 0.0 38-39 3.730400939762605E-5 2.7978007048219536E-4 0.0 0.13228934332633138 0.0 40-41 3.730400939762605E-5 2.984320751810084E-4 0.0 0.1900546018785553 0.0 42-43 3.730400939762605E-5 2.984320751810084E-4 0.0 0.25066429114734823 0.0 44-45 3.730400939762605E-5 2.984320751810084E-4 0.0 0.31943423247187186 0.0 46-47 3.730400939762605E-5 3.077580775304149E-4 0.0 0.40007617478718993 0.0 48-49 3.730400939762605E-5 3.1708407987982143E-4 0.0 0.48806700695384037 0.0 50-51 3.730400939762605E-5 3.5438808927744744E-4 0.0 0.580375778208266 0.0 52-53 3.730400939762605E-5 3.5438808927744744E-4 0.0 0.6726658974579929 0.0 54-55 3.730400939762605E-5 3.5438808927744744E-4 0.0 0.7722023205332086 0.0 56-57 3.730400939762605E-5 3.6371409162685395E-4 0.0 0.8768960229076461 0.0 58-59 3.730400939762605E-5 4.2899610807269956E-4 0.0 0.9896194133049225 0.0 60-61 3.730400939762605E-5 4.383221104221061E-4 0.0 1.1067540028134686 0.0 62-63 3.730400939762605E-5 4.476481127715126E-4 0.0 1.2248958005757502 0.0 64-65 3.730400939762605E-5 4.476481127715126E-4 0.0 1.3570918838785873 0.0 66-67 3.730400939762605E-5 4.476481127715126E-4 0.0 1.5044520470015597 0.0 68-69 3.730400939762605E-5 4.5697411512091906E-4 0.0 1.646123348691394 0.0 70-71 5.5956014096439074E-5 4.6630011747032557E-4 0.0 1.8158565914505926 0.0 72-73 7.46080187952521E-5 5.129301292173582E-4 0.0 2.006675925521799 0.0 74-75 9.326002349406512E-5 5.222561315667647E-4 0.0 2.1780971747062403 0.0 76-77 9.326002349406512E-5 5.222561315667647E-4 0.0 2.3514862103864065 0.0 78-79 9.326002349406512E-5 5.315821339161712E-4 0.0 2.552759993091297 0.0 80-81 9.326002349406512E-5 5.688861433137972E-4 0.0 2.7919719533535745 0.0 82-83 9.326002349406512E-5 5.782121456632038E-4 0.0 3.0444827929661056 0.0 84-85 9.326002349406512E-5 5.968641503620168E-4 0.0 3.297907580808878 0.0 86-87 9.326002349406512E-5 6.248421574102363E-4 0.0 3.5948661476186796 0.0 88 9.326002349406512E-5 6.528201644584558E-4 0.0 3.845390548730787 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5200 0.0 44.413845 1 GTATCAA 8255 0.0 35.979576 1 ATCAACG 9195 0.0 30.958258 3 TCAACGC 9175 0.0 30.802536 4 CAACGCA 9925 0.0 28.629642 5 TATCAAC 10325 0.0 27.917194 2 AACGCAG 10990 0.0 26.041595 6 GTACATG 14050 0.0 24.857258 1 TACCTGG 4075 0.0 23.368929 2 TACATGG 14095 0.0 23.319725 2 GTACCTG 4545 0.0 22.646826 1 ACGCAGA 12445 0.0 22.626225 7 GTGGTAT 3345 0.0 22.351255 1 CGCAGAG 12690 0.0 22.101744 8 TATAACG 535 0.0 22.010382 2 ACATGGG 14815 0.0 21.045979 3 TCTGTCG 1715 0.0 20.04684 8 TGGTATC 3550 0.0 19.469683 2 CATGGGG 10080 0.0 18.89452 4 ATAACGC 555 0.0 18.44975 3 >>END_MODULE