FastQCFastQC Report
Wed 25 May 2016
SRR2049493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361354
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191960.35804388219841476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182030.33952244153249345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115790.215971562407556No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57810.10782723916383809No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.10359323409720753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112850.057.4495621
GTATCAA192000.040.1667751
GTGGTAT44800.032.6841131
ATCAACG247650.030.855623
TCAACGC250950.030.5436174
TATCAAC254050.030.1153532
CAACGCA261300.029.4609285
TGGTATC49800.028.439622
AACGCAG276050.028.0399866
TATAACG5450.025.0373342
ACGCAGA313700.024.5098537
CGCAGAG313450.024.4244468
TACCTGG42100.023.6941072
GTACCTG44800.023.5409681
GCAGAGT335800.022.3369649
ATAACGC5800.021.092693
GTACATG143500.020.8997861
GAGTACT254800.020.74110612-13
CAGAGTA327650.020.71950110-11
AGAGTAC313500.020.4628910-11
Produced by FastQC (version 0.11.5)