##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049493_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5361354
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.774298059781167	31.0	31.0	34.0	30.0	34.0
2	31.811414430011524	31.0	31.0	34.0	30.0	34.0
3	31.836148480402525	33.0	31.0	34.0	30.0	34.0
4	35.552166859341874	37.0	35.0	37.0	33.0	37.0
5	35.686685117229715	37.0	35.0	37.0	33.0	37.0
6	35.771202573081354	37.0	35.0	37.0	33.0	37.0
7	35.7914870012314	37.0	35.0	37.0	35.0	37.0
8	35.79396902349668	37.0	35.0	37.0	35.0	37.0
9	37.5265270676027	39.0	37.0	39.0	35.0	39.0
10-11	37.49298162367193	39.0	37.0	39.0	34.5	39.0
12-13	37.481030724701256	39.0	37.0	39.0	34.5	39.0
14-15	38.75845681893044	40.0	38.0	41.0	34.5	41.0
16-17	38.77511893450796	40.0	38.0	41.0	35.0	41.0
18-19	38.84965915326613	40.0	38.0	41.0	35.0	41.0
20-21	38.876551613640885	40.0	38.0	41.0	35.0	41.0
22-23	38.84180833796836	40.0	38.0	41.0	35.0	41.0
24-25	38.79757063234399	40.0	38.0	41.0	35.0	41.0
26-27	38.7022777268578	40.0	38.0	41.0	34.5	41.0
28-29	38.60600363266444	40.0	38.0	41.0	34.0	41.0
30-31	38.46962828792876	40.0	38.0	41.0	34.0	41.0
32-33	38.30169403102276	40.0	38.0	41.0	34.0	41.0
34-35	38.17717604545419	40.0	38.0	41.0	34.0	41.0
36-37	38.020650287222225	40.0	38.0	41.0	33.5	41.0
38-39	37.91393162995766	40.0	37.0	41.0	33.0	41.0
40-41	37.78140969613273	40.0	37.0	41.0	33.0	41.0
42-43	37.62988771120131	40.0	37.0	41.0	33.0	41.0
44-45	37.4283784469371	40.0	37.0	41.0	33.0	41.0
46-47	37.16004716345908	40.0	37.0	41.0	32.0	41.0
48-49	36.906022806925264	40.0	37.0	41.0	31.0	41.0
50-51	36.68891934761256	40.0	36.0	41.0	31.0	41.0
52-53	36.57914372003789	40.0	36.0	41.0	31.0	41.0
54-55	36.443229173078294	39.5	35.5	41.0	30.5	41.0
56-57	36.267651697686816	39.0	35.0	41.0	30.0	41.0
58-59	36.095478119892846	39.0	35.0	41.0	30.0	41.0
60-61	35.89804226693481	39.0	35.0	41.0	29.5	41.0
62-63	35.68416905505587	38.5	35.0	41.0	29.0	41.0
64-65	35.39659729613079	38.0	35.0	40.0	29.0	41.0
66-67	35.13821275371856	37.5	35.0	40.0	29.0	41.0
68-69	34.82629956910139	37.0	34.5	40.0	28.0	41.0
70-71	34.531325855371605	36.5	34.0	39.0	28.0	41.0
72-73	34.20472356423396	36.0	34.0	39.0	28.0	41.0
74-75	33.87578818708856	35.5	34.0	39.0	28.0	40.5
76-77	33.59097076969736	35.0	34.0	37.5	27.5	40.0
78-79	33.269312938485314	35.0	34.0	37.0	27.0	39.0
80-81	32.968722919620674	35.0	34.0	37.0	26.5	39.0
82-83	32.685286776437444	35.0	33.0	36.0	26.0	39.0
84-85	32.41701946933554	35.0	33.0	36.0	26.0	37.0
86-87	32.14663571926047	35.0	33.0	35.5	26.0	37.0
88-89	31.94754179634473	35.0	33.0	35.0	25.5	36.5
90-91	31.804573807288236	35.0	33.0	35.0	25.0	36.0
92-93	31.648441233315317	35.0	33.0	35.0	25.0	36.0
94-95	31.47029668251714	35.0	33.0	35.0	25.0	36.0
96-97	31.301816108393513	35.0	33.0	35.0	24.5	36.0
98-99	31.127655439278957	35.0	33.0	35.0	24.0	35.0
100	31.019062721842282	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39751279101092507
1101	2	0.42412380125025706
1101	3	0.47099855054338846
1101	4	0.4022664743744997
1101	5	0.30295910056411657
1101	6	0.2983308177381403
1101	7	0.28965939368647753
1101	8	0.326269039115175
1101	9	0.339188900523979
1101	10-11	0.3524604776704905
1101	12-13	0.3382403235883231
1101	14-15	0.47106551192753443
1101	16-17	0.4531976234707571
1101	18-19	0.44818328550787356
1101	20-21	0.43226241404732235
1101	22-23	0.4644074512721019
1101	24-25	0.5233468987786978
1101	26-27	0.48114178885421666
1101	28-29	0.461778047672631
1101	30-31	0.5377090122247807
1101	32-33	0.5419786719081969
1101	34-35	0.570349069818711
1101	36-37	0.5543480679750701
1101	38-39	0.5911135232422495
1101	40-41	0.6328008338812765
1101	42-43	0.5979680676275407
1101	44-45	0.6665399406196855
1101	46-47	0.6927819448373569
1101	48-49	0.6300194373083627
1101	50-51	0.6848078536680617
1101	52-53	0.7038850627186335
1101	54-55	0.6636078644729295
1101	56-57	0.6489261546385379
1101	58-59	0.6212058595242382
1101	60-61	0.6632748958917105
1101	62-63	0.6843113321825882
1101	64-65	0.6691042346675715
1101	66-67	0.6968735783345679
1101	68-69	0.6678350541899789
1101	70-71	0.7109649423681716
1101	72-73	0.6547800471548797
1101	74-75	0.6598232199572749
1101	76-77	0.6024610746654062
1101	78-79	0.592989328772731
1101	80-81	0.6285157450129617
1101	82-83	0.6446703711764741
1101	84-85	0.6377448054157284
1101	86-87	0.6536985389460739
1101	88-89	0.6684849299102105
1101	90-91	0.7014627501161108
1101	92-93	0.7296147636594164
1101	94-95	0.6761575013325078
1101	96-97	0.7070991078047371
1101	98-99	0.7977097588899547
1101	100	0.8342492674425799
1102	1	0.2747718608396781
1102	2	0.2753558516887473
1102	3	0.37412933480491617
1102	4	0.3505095627792727
1102	5	0.2578906546387998
1102	6	0.2607376448188603
1102	7	0.27241169481769845
1102	8	0.29448660791285874
1102	9	0.3383762993794619
1102	10-11	0.31196725004035386
1102	12-13	0.29608847538588634
1102	14-15	0.3879881843283641
1102	16-17	0.43117041934436884
1102	18-19	0.44595109807365674
1102	20-21	0.4075857573599748
1102	22-23	0.37876268667898927
1102	24-25	0.39519145476198503
1102	26-27	0.3777873539122609
1102	28-29	0.40698415559049295
1102	30-31	0.48745444569768637
1102	32-33	0.4807129296846284
1102	34-35	0.49044309533265107
1102	36-37	0.5266442936536464
1102	38-39	0.5313230731908334
1102	40-41	0.5216018635211412
1102	42-43	0.5116373713482574
1102	44-45	0.5363629003128381
1102	46-47	0.5116094961081856
1102	48-49	0.5352785867394303
1102	50-51	0.5733407238810031
1102	52-53	0.526519036896083
1102	54-55	0.5682216090998153
1102	56-57	0.5167429943265773
1102	58-59	0.5115629815339915
1102	60-61	0.5573317506216426
1102	62-63	0.5569626485429353
1102	64-65	0.5730246329813298
1102	66-67	0.5710302475834155
1102	68-69	0.5761732498747918
1102	70-71	0.5472866064426967
1102	72-73	0.5708068175255931
1102	74-75	0.5230659605390926
1102	76-77	0.5533519018730146
1102	78-79	0.5174293486901007
1102	80-81	0.4384773260534516
1102	82-83	0.4649836895287649
1102	84-85	0.5077465817784841
1102	86-87	0.5127834050524172
1102	88-89	0.5113477726369133
1102	90-91	0.52233448256084
1102	92-93	0.5356386269367768
1102	94-95	0.5090755106324742
1102	96-97	0.5178394694979289
1102	98-99	0.5550017209831637
1102	100	0.6235149721033686
1103	1	0.22690264606397648
1103	2	0.24956170942571632
1103	3	0.35916522303221754
1103	4	0.2934550553546984
1103	5	0.23114414116437132
1103	6	0.2314838297811761
1103	7	0.27290748414434773
1103	8	0.3002475364260988
1103	9	0.3369697598279515
1103	10-11	0.30202145453975504
1103	12-13	0.2808828994429149
1103	14-15	0.35953077150914226
1103	16-17	0.3940712002458895
1103	18-19	0.3413572635426334
1103	20-21	0.3650974222068655
1103	22-23	0.3692403923114682
1103	24-25	0.33321059049156787
1103	26-27	0.37915603625663863
1103	28-29	0.3587857113849964
1103	30-31	0.3441273885930869
1103	32-33	0.3095367320820941
1103	34-35	0.36858555576689156
1103	36-37	0.3959940084401836
1103	38-39	0.36282957264712934
1103	40-41	0.37032355238937953
1103	42-43	0.34826772480123225
1103	44-45	0.41772693649939185
1103	46-47	0.3709001053806915
1103	48-49	0.38987272507327475
1103	50-51	0.4284098916820298
1103	52-53	0.3562864002231265
1103	54-55	0.32404587206235647
1103	56-57	0.34947857873079613
1103	58-59	0.2957650862934358
1103	60-61	0.3071556968866176
1103	62-63	0.25723448118079517
1103	64-65	0.3262510358229278
1103	66-67	0.31534510099618984
1103	68-69	0.311381385862866
1103	70-71	0.34698170149212615
1103	72-73	0.3217670707324203
1103	74-75	0.2724674099080957
1103	76-77	0.24358379389150286
1103	78-79	0.22004869948507633
1103	80-81	0.2109352407549423
1103	82-83	0.23583358433534585
1103	84-85	0.286140778293948
1103	86-87	0.35394371174204053
1103	88-89	0.31316054213111855
1103	90-91	0.31579357743502
1103	92-93	0.2968085959063558
1103	94-95	0.3279744883629867
1103	96-97	0.3164275830167611
1103	98-99	0.3153910854809929
1103	100	0.31816419090142034
1104	1	0.30891928019577364
1104	2	0.26206098224983876
1104	3	0.3469561683817197
1104	4	0.2714058437437856
1104	5	0.2162912315608736
1104	6	0.22893571421668923
1104	7	0.17967975457394658
1104	8	0.23499436052622968
1104	9	0.25852483097639833
1104	10-11	0.27835735814088736
1104	12-13	0.2953634819557891
1104	14-15	0.373513450505925
1104	16-17	0.39378450382088204
1104	18-19	0.35098148513835525
1104	20-21	0.36211675112853214
1104	22-23	0.34901283778501835
1104	24-25	0.3955863673306155
1104	26-27	0.36127752067108077
1104	28-29	0.3988776564154648
1104	30-31	0.4239322993715433
1104	32-33	0.4174696860752789
1104	34-35	0.40656053856901764
1104	36-37	0.3828987122702543
1104	38-39	0.4607507822608241
1104	40-41	0.41872891113425936
1104	42-43	0.3737245869023411
1104	44-45	0.4151289294960705
1104	46-47	0.5116280064732948
1104	48-49	0.4726674047806796
1104	50-51	0.524362867729181
1104	52-53	0.5122192553464728
1104	54-55	0.4735199380359276
1104	56-57	0.49183854944028127
1104	58-59	0.4756480494562041
1104	60-61	0.4954124978397303
1104	62-63	0.5055639984168891
1104	64-65	0.4956478294142812
1104	66-67	0.4703617797368054
1104	68-69	0.389696205668038
1104	70-71	0.3332422514118747
1104	72-73	0.3860880267280393
1104	74-75	0.463895154191583
1104	76-77	0.40988647044653703
1104	78-79	0.35751908092697704
1104	80-81	0.363037379030267
1104	82-83	0.42228610868563266
1104	84-85	0.42125178381056827
1104	86-87	0.4704914498272501
1104	88-89	0.4410274737724116
1104	90-91	0.44066878362035666
1104	92-93	0.41116717591644303
1104	94-95	0.3949865820477605
1104	96-97	0.430801097849713
1104	98-99	0.47291785794446994
1104	100	0.5018541643265664
1105	1	0.20599194457413006
1105	2	0.21532124527361773
1105	3	0.28845024901965743
1105	4	0.228313986326647
1105	5	0.14234806424948232
1105	6	0.2128277349428629
1105	7	0.2021493083353647
1105	8	0.22502060235899535
1105	9	0.2197303409656186
1105	10-11	0.2404808210035938
1105	12-13	0.19324646766170872
1105	14-15	0.2311670091442295
1105	16-17	0.23929854965992092
1105	18-19	0.24655017504395715
1105	20-21	0.23866941310250667
1105	22-23	0.2538281823946207
1105	24-25	0.24369282447122487
1105	26-27	0.20858430930317695
1105	28-29	0.25422667073986815
1105	30-31	0.23073141334293723
1105	32-33	0.26908621212680117
1105	34-35	0.22709689394419996
1105	36-37	0.28619944512826123
1105	38-39	0.31738782419660083
1105	40-41	0.3079915051284843
1105	42-43	0.3151238178231921
1105	44-45	0.4120904595635011
1105	46-47	0.3552358659320518
1105	48-49	0.4100784720013735
1105	50-51	0.3842659039209195
1105	52-53	0.37430893222245487
1105	54-55	0.3923014159118523
1105	56-57	0.35931556903295814
1105	58-59	0.35231768264677754
1105	60-61	0.35375492323627356
1105	62-63	0.37673742966768486
1105	64-65	0.395549924490453
1105	66-67	0.39925813649444564
1105	68-69	0.4255655994025105
1105	70-71	0.40844878400956475
1105	72-73	0.3445908131822648
1105	74-75	0.36564118153701486
1105	76-77	0.3179623469256825
1105	78-79	0.2596135587977315
1105	80-81	0.2790723488085831
1105	82-83	0.30848943993636624
1105	84-85	0.2649136843754363
1105	86-87	0.31982791517744147
1105	88-89	0.3097923500340478
1105	90-91	0.28704078932349475
1105	92-93	0.2706189397342307
1105	94-95	0.2790016797419561
1105	96-97	0.28728556593075893
1105	98-99	0.3136484383759388
1105	100	0.3019423053317851
1106	1	0.09477945790764153
1106	2	0.11340959950175744
1106	3	0.2735942396481654
1106	4	0.20884687338536168
1106	5	0.14291373480502756
1106	6	0.14914952378973823
1106	7	0.11338759684179678
1106	8	0.15136655495383167
1106	9	0.13444548451405325
1106	10-11	0.16197820380195083
1106	12-13	0.1531018812352869
1106	14-15	0.21280910534599684
1106	16-17	0.18863340169325227
1106	18-19	0.22273958920794001
1106	20-21	0.16350545222963575
1106	22-23	0.17281891214093292
1106	24-25	0.18828473790402
1106	26-27	0.2409978151022898
1106	28-29	0.20718695274267418
1106	30-31	0.21940870888230535
1106	32-33	0.23785336226967502
1106	34-35	0.16946102898914006
1106	36-37	0.1963104050553497
1106	38-39	0.28552049739404595
1106	40-41	0.2890027215713218
1106	42-43	0.273993238870645
1106	44-45	0.26446631668960663
1106	46-47	0.27271928553530955
1106	48-49	0.32317308674322476
1106	50-51	0.3759805969829415
1106	52-53	0.3310992388120795
1106	54-55	0.26214592266763503
1106	56-57	0.27464009822391233
1106	58-59	0.28536379930447
1106	60-61	0.23973276691121015
1106	62-63	0.250838646663361
1106	64-65	0.2554889292612472
1106	66-67	0.31197741263905954
1106	68-69	0.2566596482957877
1106	70-71	0.2825778365535143
1106	72-73	0.28749889474308077
1106	74-75	0.29560719428067017
1106	76-77	0.2705262189769826
1106	78-79	0.27350598203566534
1106	80-81	0.23218206609687542
1106	82-83	0.22296645715585584
1106	84-85	0.28232308797274186
1106	86-87	0.37472147798922606
1106	88-89	0.29421748188057606
1106	90-91	0.2958119463612725
1106	92-93	0.3588105231277616
1106	94-95	0.3541496869073164
1106	96-97	0.3567010818673566
1106	98-99	0.4131421666404975
1106	100	0.3855280585567513
1107	1	0.11591859773496438
1107	2	0.1102597188760619
1107	3	0.22283021767877287
1107	4	0.19174989816446697
1107	5	0.1619491796254735
1107	6	0.16287210288329135
1107	7	0.16192430599011232
1107	8	0.1935836572192855
1107	9	0.17674472765945382
1107	10-11	0.15648671287414118
1107	12-13	0.12332223365478967
1107	14-15	0.19014108971838084
1107	16-17	0.1717574577373142
1107	18-19	0.17023843570783725
1107	20-21	0.2225935769667018
1107	22-23	0.2337003226900265
1107	24-25	0.25415112573109866
1107	26-27	0.2445655137776015
1107	28-29	0.2519554889471891
1107	30-31	0.2746699295108783
1107	32-33	0.2452520735492385
1107	34-35	0.1925046961436081
1107	36-37	0.24513662523721536
1107	38-39	0.24173776063123142
1107	40-41	0.25162254555987573
1107	42-43	0.1937646822351411
1107	44-45	0.21437728573160797
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2216	92-93	-1.184267901889907
2216	94-95	-1.279240737477842
2216	96-97	-1.416207266295551
2216	98-99	-1.4303295132899123
2216	100	-1.4048018512659866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	328.0
3	34.0
4	48.0
5	132.0
6	379.0
7	988.0
8	2337.0
9	3955.0
10	5652.0
11	7785.0
12	11498.0
13	16320.0
14	17929.0
15	21879.0
16	27353.0
17	39119.0
18	38726.0
19	20988.0
20	15510.0
21	14711.0
22	15478.0
23	16480.0
24	19323.0
25	23440.0
26	29862.0
27	37898.0
28	49630.0
29	64932.0
30	84168.0
31	109015.0
32	139121.0
33	182558.0
34	248596.0
35	365199.0
36	590792.0
37	1089151.0
38	1470758.0
39	577445.0
40	1837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43227087209388	20.327388634171168	14.374384473646934	22.865956020088017
2	16.13766694737981	21.06753062897463	38.93950118860099	23.855301235044564
3	18.23601584078046	25.543948615946128	30.79920885627357	25.42082668699985
4	13.044207368598162	17.805114391928093	37.65406957689935	31.496608662574392
5	12.50686636528336	36.883624224741894	34.869664496102516	15.739844913872231
6	29.629358826264713	37.98779656413947	17.806503935536252	14.576340674059557
7	25.883013880448857	32.86140031044397	22.645529468861785	18.610056340245393
8	24.848946739946662	34.984017097173584	20.993838496767793	19.173197666111957
9	24.414186725358352	16.836617642944407	22.435655198783888	36.31354043291335
10-11	23.455931095018162	26.855426819419126	28.68771582700937	21.000926258553342
12-13	24.632504145875117	24.42773307409529	29.058103430234866	21.881659349794724
14-15	21.921895820308013	26.428277792616488	27.282567904513638	24.36725848256186
16-17	20.5349059211535	29.526020852195174	27.837883862919703	22.101189363731624
18-19	20.294341690550556	27.48089381898677	31.22221550750053	21.00254898296214
20-21	21.495101797046047	26.949059883007166	30.99121042930573	20.564627890641056
22-23	21.137263086899317	26.943175175524694	31.08978627413896	20.829775463437034
24-25	20.968490422382107	26.774710269085013	31.19404165440297	21.062757654129907
26-27	20.765537959254324	26.708971278524047	31.253392333354597	21.272098428867036
28-29	20.859296952930496	26.783068362658046	31.286590611167192	21.071044073244266
30-31	21.27004431074654	27.28362431000232	30.733582394179216	20.712748985071926
32-33	20.726514438287726	26.958328145743078	31.255518080558964	21.059639335410235
34-35	20.82136340931787	26.60924833540184	31.210884414646	21.358503840634288
36-37	21.52034728540589	27.054882031665883	30.49212941357724	20.932641269350988
38-39	21.256915697042203	27.09559003192104	30.64695037857974	21.000543892457017
40-41	21.026013204873248	26.903716859584353	30.727359170836323	21.34291076470608
42-43	20.99751294169346	27.01911681265591	30.524444011717932	21.458926233932697
44-45	21.393784107522094	27.231917534264667	30.18139634129737	21.192902016915877
46-47	21.35534232583784	27.684732252337753	29.591032414572886	21.368893007251526
48-49	21.380820964256415	27.387988183581985	29.596077781843917	21.635113070317686
50-51	21.472337025311145	27.33884947720296	29.34607563686337	21.84273786062252
52-53	21.420447148239045	27.514066409343606	29.247732942088884	21.817753500328465
54-55	21.54908069864441	27.96116428797651	29.043372252606336	21.446382760772746
56-57	21.22501144300488	27.70139781853614	29.034120858275724	22.03946988018325
58-59	21.080126147426412	27.25877979149266	29.492649793540654	22.168444267540277
60-61	21.549509694752484	27.646635532740422	29.01851845634517	21.785336316161924
62-63	21.3261146344748	27.638083588586014	29.142423723559386	21.8933780533798
64-65	21.26013316785275	27.331258111290545	29.404484389577707	22.004124331279
66-67	21.186504379304182	27.44322609549752	29.14186416341842	22.228405361779878
68-69	21.697638637112686	27.70398628853575	28.802272486068347	21.79610258828322
70-71	21.69944383452389	27.319311502280954	28.931646744460448	22.049597918734705
72-73	21.379412737901657	27.5228235255497	29.233100444402666	21.864663292145977
74-75	21.40028433115963	27.504870971027096	29.03646168486543	22.05838301294785
76-77	21.475936862218013	27.67172247906033	28.667114687819534	22.185225970902124
78-79	21.583648366045256	27.734862575605984	28.822602076940353	21.858886981408407
80-81	21.37678196039028	27.537569696953305	28.9313828616023	22.154265481054114
82-83	21.23471732489291	27.519796588185365	29.05318175557027	22.19230433135146
84-85	21.36256997765863	27.517628942241085	28.821488004709256	22.29831307539103
86-87	21.523098456098964	27.63826078263066	28.71934962697856	22.11929113429182
88-89	21.20498426888163	27.49966053313458	28.834884435779955	22.460470762203833
90-91	21.041286243203672	27.456843891930205	28.775905322353513	22.725964542512614
92-93	21.247329946602665	27.587042062060885	28.630983512257064	22.53464447907939
94-95	21.173448285155974	27.374180437144336	28.816400294069457	22.63597098363024
96-97	21.040151424434946	27.40707851039122	28.855807693355075	22.69696237181876
98-99	21.05481779084662	27.38258676332138	28.770025847022744	22.792569598809255
100	21.199446178168653	27.468980885387495	28.549056561190206	22.782516375253646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1110.0
1	908.0
2	2573.0
3	4130.0
4	2455.0
5	1126.0
6	2029.0
7	4835.5
8	7747.5
9	8758.5
10	7064.5
11	4802.0
12	4376.0
13	4770.0
14	5396.0
15	6063.0
16	6653.0
17	7255.5
18	8034.0
19	8979.0
20	10335.5
21	12251.0
22	15042.0
23	19667.0
24	25434.5
25	32030.0
26	41087.0
27	52919.0
28	67994.5
29	83917.5
30	96681.5
31	109517.5
32	122448.0
33	133788.5
34	145243.0
35	155011.0
36	161653.5
37	168008.5
38	174179.0
39	176776.5
40	179204.0
41	179529.5
42	175810.5
43	173416.0
44	171678.5
45	169979.0
46	169048.5
47	166235.0
48	163887.5
49	165207.0
50	163958.5
51	158131.0
52	150098.5
53	143604.0
54	139451.0
55	132478.5
56	123633.0
57	113440.0
58	103191.5
59	94443.5
60	83441.5
61	70304.0
62	58742.5
63	48437.0
64	38571.0
65	30335.5
66	23763.0
67	18293.5
68	13633.0
69	10204.0
70	7579.0
71	5523.5
72	4164.5
73	3052.0
74	2198.0
75	1476.0
76	927.5
77	624.5
78	457.0
79	320.0
80	184.5
81	87.0
82	45.0
83	24.5
84	15.5
85	8.0
86	3.5
87	1.5
88	1.0
89	2.5
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	2.0
98	2.0
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05847403473077883
2	1.678680422893172E-4
3	3.730400939762604E-5
4	0.11913035401131877
5	0.001398900352410977
6	0.0014362043618086028
7	0.0
8	0.0
9	7.460801879525209E-5
10-11	0.0
12-13	1.865200469881302E-5
14-15	3.730400939762604E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.1191202819287814E-4
30-31	2.518020634339758E-4
32-33	2.7978007048219534E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.4921603759050417E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.865200469881302E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.1191202819287814E-4
80-81	3.450620869280409E-4
82-83	2.518020634339758E-4
84-85	0.0
86-87	0.0
88-89	1.1191202819287814E-4
90-91	7.460801879525209E-5
92-93	7.087761785548949E-4
94-95	6.621461668078624E-4
96-97	0.0
98-99	2.7978007048219534E-5
100	0.0011750762960252206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5361354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.6538045259613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85847099201074	46.61758675713686
2	14.097510359655866	17.101352753145626
3	4.0395818073238585	7.350480159241525
4	1.7218171187846267	4.177390358088665
5	0.9138195170871783	2.7713315180707037
6	0.5413744176760716	1.9701850863048347
7	0.37065754680388296	1.5737253272940548
8	0.2524343289810542	1.2248881956527258
9	0.188656225681773	1.0298446051597112
>10	0.9471626958296027	10.06370522165555
>50	0.046835651902688236	1.9336538312272933
>100	0.020141457244078682	2.2090326693765117
>500	9.227309379610792E-4	0.3920865725599551
>1k	4.6136256053584305E-4	0.4595619610773676
>5k	6.151500806322459E-5	0.21146118488114024
>10k+	9.227251209483689E-5	0.9137137991273478
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19196	0.35804388219841476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18203	0.33952244153249345	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11579	0.215971562407556	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5781	0.10782723916383809	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5554	0.10359323409720753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8652004698813025E-5	0.0	0.0	0.0
3	0.0	1.8652004698813025E-5	0.0	1.8652004698813025E-5	0.0
4	0.0	1.8652004698813025E-5	0.0	3.730400939762605E-5	0.0
5	0.0	1.8652004698813025E-5	0.0	3.730400939762605E-5	0.0
6	0.0	1.8652004698813025E-5	0.0	3.730400939762605E-5	0.0
7	0.0	1.8652004698813025E-5	0.0	3.730400939762605E-5	0.0
8	0.0	1.8652004698813025E-5	0.0	3.730400939762605E-5	0.0
9	0.0	1.8652004698813025E-5	0.0	3.730400939762605E-5	0.0
10-11	0.0	5.5956014096439074E-5	0.0	4.6630011747032565E-5	0.0
12-13	0.0	9.326002349406513E-5	0.0	8.393402114465861E-5	0.0
14-15	0.0	1.2123803054228465E-4	0.0	1.2123803054228465E-4	0.0
16-17	0.0	1.492160375905042E-4	0.0	1.3056403289169117E-4	0.0
18-19	0.0	1.7719404463872372E-4	0.0	1.9584604933753675E-4	0.0
20-21	0.0	1.9584604933753675E-4	0.0	2.7978007048219536E-4	0.0
22-23	0.0	2.0517205168694327E-4	0.0	5.222561315667647E-4	0.0
24-25	0.0	2.0517205168694327E-4	0.0	9.978822513864968E-4	0.0
26-27	0.0	2.0517205168694327E-4	0.0	0.0018838524745801153	0.0
28-29	0.0	2.0517205168694327E-4	0.0	0.004560415148859785	0.0
30-31	0.0	2.0517205168694327E-4	0.0	0.011312440849830099	0.0
32-33	0.0	2.0517205168694327E-4	0.0	0.025758418489060787	0.0
34-35	0.0	2.0517205168694327E-4	0.0	0.04923196640251698	0.0
36-37	0.0	2.3315005873516279E-4	0.0	0.0852116834665273	0.0
38-39	0.0	2.7045406813278885E-4	0.0	0.13406128377271861	0.0
40-41	0.0	2.7978007048219536E-4	0.0	0.19253531850349742	0.0
42-43	0.0	2.7978007048219536E-4	0.0	0.25385378395084524	0.0
44-45	0.0	2.891060728316019E-4	0.0	0.32301541737404393	0.0
46-47	0.0	3.1708407987982143E-4	0.0	0.4043101798538205	0.0
48-49	0.0	3.5438808927744744E-4	0.0	0.4929351801802306	0.0
50-51	0.0	3.916920986750735E-4	0.0	0.5855983395239337	0.0
52-53	0.0	3.916920986750735E-4	0.0	0.678289476874685	0.0
54-55	0.0	4.1034410337388653E-4	0.0	0.7816495609131574	0.0
56-57	0.0	4.2899610807269956E-4	0.0	0.9014700390983322	0.0
58-59	0.0	4.2899610807269956E-4	0.0	1.0315397938655049	0.0
60-61	0.0	4.383221104221061E-4	0.0	1.1667295239225017	0.0
62-63	0.0	4.476481127715126E-4	0.0	1.3028052242026922	0.0
64-65	0.0	4.476481127715126E-4	0.0	1.4554439046554286	0.0
66-67	0.0	4.5697411512091906E-4	0.0	1.631005898883006	0.0
68-69	0.0	4.6630011747032557E-4	0.0	1.8184678721084264	0.0
70-71	0.0	4.849521221691386E-4	0.0	2.0192567026911483	0.0
72-73	0.0	5.129301292173582E-4	0.0	2.2368789675145493	0.0
74-75	0.0	5.409081362655777E-4	0.0	2.4568886889393986	0.0
76-77	0.0	5.688861433137972E-4	0.0	2.687175664953293	0.0
78-79	0.0	5.875381480126103E-4	0.0	2.9443495057405276	0.0
80-81	0.0	6.155161550608298E-4	0.0	3.2202033292336227	0.0
82-83	0.0	6.248421574102363E-4	0.0	3.512293722817035	0.0
84-85	0.0	6.341681597596429E-4	0.0	3.8043747903980973	0.0
86-87	0.0	6.341681597596429E-4	0.0	4.114538976534659	0.0
88	0.0	6.341681597596429E-4	0.0	4.368765800579481	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11285	0.0	57.449562	1
GTATCAA	19200	0.0	40.166775	1
GTGGTAT	4480	0.0	32.684113	1
ATCAACG	24765	0.0	30.85562	3
TCAACGC	25095	0.0	30.543617	4
TATCAAC	25405	0.0	30.115353	2
CAACGCA	26130	0.0	29.460928	5
TGGTATC	4980	0.0	28.43962	2
AACGCAG	27605	0.0	28.039986	6
TATAACG	545	0.0	25.037334	2
ACGCAGA	31370	0.0	24.509853	7
CGCAGAG	31345	0.0	24.424446	8
TACCTGG	4210	0.0	23.694107	2
GTACCTG	4480	0.0	23.540968	1
GCAGAGT	33580	0.0	22.336964	9
ATAACGC	580	0.0	21.09269	3
GTACATG	14350	0.0	20.899786	1
GAGTACT	25480	0.0	20.741106	12-13
CAGAGTA	32765	0.0	20.719501	10-11
AGAGTAC	31350	0.0	20.46289	10-11
>>END_MODULE