FastQCFastQC Report
Wed 25 May 2016
SRR2049492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7471264
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.047.9655151
GTATCAA105700.035.1714941
TCAACGC107350.033.193364
ATCAACG108100.032.9156343
CAACGCA117000.030.4556165
TATCAAC126250.028.6709272
AACGCAG127650.027.9144526
ACGCAGA148150.023.9132297
GTACATG169750.023.6828581
CGCAGAG149050.023.3218928
TACATGG173400.021.850622
ACATGGG179550.020.045653
GCAGAGT173450.019.1289529
GTCTCGC20000.018.459941
GTGGTAT39450.018.1973781
GTCGTAC3258.011739E-817.35551
CAGAGTA168450.017.09377110-11
AGAGTAC150200.016.47720710-11
TCTGTCG22000.016.4375368
CATGGGG123050.016.3749284
Produced by FastQC (version 0.11.5)