##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7471264 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.422178094630308 31.0 31.0 34.0 27.0 34.0 2 30.51306552679707 31.0 31.0 34.0 27.0 34.0 3 30.716781390672313 31.0 31.0 34.0 27.0 34.0 4 34.2917981482116 37.0 35.0 37.0 30.0 37.0 5 34.31371840159844 37.0 35.0 37.0 30.0 37.0 6 34.3213133681262 37.0 35.0 37.0 30.0 37.0 7 30.319631591120324 36.0 32.0 37.0 2.0 37.0 8 32.118645118148685 36.0 32.0 37.0 17.0 37.0 9 34.799756507064934 38.0 33.0 39.0 28.0 39.0 10-11 35.386009114388145 38.5 35.0 39.0 30.0 39.0 12-13 35.559809892944486 39.0 35.0 39.0 30.0 39.0 14-15 36.692312237929215 40.0 37.0 41.0 30.0 41.0 16-17 36.696951078157596 40.0 37.0 41.0 30.0 41.0 18-19 36.69410671340218 40.0 36.5 41.0 30.0 41.0 20-21 36.7739802796421 40.0 37.0 41.0 30.0 41.0 22-23 36.70909433798619 40.0 37.0 41.0 30.0 41.0 24-25 36.69667441814397 40.0 37.0 41.0 30.0 41.0 26-27 36.71915535309688 40.0 37.0 41.0 30.0 41.0 28-29 36.68573624222086 40.0 36.0 41.0 30.0 41.0 30-31 36.58484561648471 40.0 36.0 41.0 30.0 41.0 32-33 36.53236139159318 40.0 36.0 41.0 30.0 41.0 34-35 36.44831323053234 40.0 36.0 41.0 30.0 41.0 36-37 36.28339769549035 40.0 36.0 41.0 29.5 41.0 38-39 36.142830182416255 40.0 36.0 41.0 29.0 41.0 40-41 36.03908923577055 40.0 35.5 41.0 28.5 41.0 42-43 35.87328763914647 39.0 35.0 41.0 28.0 41.0 44-45 35.711375009636924 39.0 35.0 41.0 27.5 41.0 46-47 35.548389536228406 39.0 35.0 41.0 27.0 41.0 48-49 35.44833979899519 39.0 35.0 40.0 27.0 41.0 50-51 35.32179146661127 39.0 35.0 40.0 27.0 41.0 52-53 35.18010058271264 39.0 34.5 40.0 26.5 41.0 54-55 35.03473234247913 39.0 34.0 40.0 26.0 41.0 56-57 34.822874148203034 38.0 34.0 40.0 26.0 41.0 58-59 34.629536983300284 38.0 34.0 40.0 26.0 41.0 60-61 34.39328900705423 38.0 34.0 40.0 25.0 41.0 62-63 34.12973059177135 38.0 33.5 40.0 24.5 41.0 64-65 33.771839343382865 37.0 33.0 40.0 23.0 41.0 66-67 30.374653472290632 36.0 29.0 39.0 6.5 41.0 68-69 30.671007074572657 35.0 28.5 39.0 8.5 41.0 70-71 30.806707606637914 35.0 28.0 39.0 11.5 40.5 72-73 31.961018309619362 35.0 30.5 38.5 18.5 40.0 74-75 32.017350477777256 35.0 32.0 38.0 18.0 40.0 76-77 31.678843968035395 35.0 31.5 37.0 15.5 39.5 78-79 28.368833774311817 34.0 26.0 36.0 4.5 39.0 80-81 29.99940023535509 34.0 28.0 36.0 7.0 38.5 82-83 30.33987381519379 34.5 30.5 36.0 2.0 37.5 84-85 28.542595938250876 34.0 28.0 35.5 2.0 37.0 86-87 28.10448834360558 34.0 25.5 35.0 2.0 37.0 88-89 29.17306121159686 34.0 29.5 35.0 2.0 36.0 90-91 29.239637964874483 34.0 30.0 35.0 2.0 36.0 92-93 28.995859067488446 34.0 30.0 35.0 2.0 36.0 94-95 28.693426908753324 34.0 30.0 35.0 2.0 35.5 96-97 28.411630950264907 34.0 29.0 35.0 2.0 35.0 98-99 28.104361390522406 34.0 29.0 35.0 2.0 35.0 100 27.827145982259495 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5812579539869667 1101 2 0.5736242377472252 1101 3 0.571860951925963 1101 4 0.610972201661518 1101 5 0.6026712959014091 1101 6 0.5993231651214472 1101 7 -11.715355499527078 1101 8 -6.175547052662559 1101 9 -1.8747623856721063 1101 10-11 0.11741550622186736 1101 12-13 0.6218632132487443 1101 14-15 0.8375270371006991 1101 16-17 0.8771061260991644 1101 18-19 0.8639709374334714 1101 20-21 0.868359032830476 1101 22-23 0.8415645167966872 1101 24-25 0.8679570545345641 1101 26-27 0.8256265947846586 1101 28-29 0.7850987689504549 1101 30-31 0.829633079428227 1101 32-33 0.860519443170503 1101 34-35 0.8901912778773351 1101 36-37 0.9015913520504881 1101 38-39 0.9311263919930255 1101 40-41 0.9482780789248295 1101 42-43 0.9161367919530434 1101 44-45 0.8152114135194708 1101 46-47 0.8357860204390164 1101 48-49 0.7837654780891512 1101 50-51 0.8943156322826482 1101 52-53 0.9359224070911054 1101 54-55 0.8630233827006464 1101 56-57 0.8124070563108035 1101 58-59 0.8255745150689364 1101 60-61 0.8643740671537827 1101 62-63 0.7947092905935733 1101 64-65 0.6050615702403732 1101 66-67 -9.720864667746433 1101 68-69 -7.763159369453604 1101 70-71 -6.300513949937301 1101 72-73 -1.2796118684991349 1101 74-75 0.02265288644699126 1101 76-77 0.3579611519797439 1101 78-79 3.36746452105902 1101 80-81 1.4068053977059698 1101 82-83 0.7201210568679315 1101 84-85 -4.861781603171867 1101 86-87 -5.396304272917039 1101 88-89 -1.0290337604230082 1101 90-91 0.2357808926036391 1101 92-93 0.6194006960058793 1101 94-95 0.6893049403128657 1101 96-97 0.8081118049579104 1101 98-99 0.8238382620696143 1101 100 0.8333546874429842 1102 1 0.5065633407456644 1102 2 0.5559722839517036 1102 3 0.5544024350596715 1102 4 0.5485171886897433 1102 5 0.5859763285759811 1102 6 0.6107702374735737 1102 7 -11.952651197327121 1102 8 -6.279195722386113 1102 9 -1.9702053955203027 1102 10-11 0.06874410169579193 1102 12-13 0.6106662986982698 1102 14-15 0.8313341125866032 1102 16-17 0.837476549781087 1102 18-19 0.8684275576550888 1102 20-21 0.9282545758515965 1102 22-23 0.9054313030355345 1102 24-25 0.9461939264462913 1102 26-27 0.8510593291062278 1102 28-29 0.8348719740466279 1102 30-31 0.8127951950363936 1102 32-33 0.8280596253885761 1102 34-35 0.920479139113695 1102 36-37 1.007773676511249 1102 38-39 0.9610533199009481 1102 40-41 0.8743287924665708 1102 42-43 0.8645900419367436 1102 44-45 0.8227766538660646 1102 46-47 0.8882568678076268 1102 48-49 0.8121323227298305 1102 50-51 0.8578233130864845 1102 52-53 0.8578527766241706 1102 54-55 0.8730156290619533 1102 56-57 0.8892640069706061 1102 58-59 0.8235130920595282 1102 60-61 0.7813654038464293 1102 62-63 0.7734870979623878 1102 64-65 0.6766203134845554 1102 66-67 -9.83610056560271 1102 68-69 -6.359719714805326 1102 70-71 -5.812752716203445 1102 72-73 -0.7360786853411447 1102 74-75 0.2642738727030931 1102 76-77 0.5144824512809585 1102 78-79 3.531214888204161 1102 80-81 1.5254201630314626 1102 82-83 0.8245983283960534 1102 84-85 -4.941712539049412 1102 86-87 -5.442447329214474 1102 88-89 -0.9971784049567205 1102 90-91 0.3189149459426055 1102 92-93 0.7232699142504337 1102 94-95 0.770091442663869 1102 96-97 0.8624450466949085 1102 98-99 0.8673027760684064 1102 100 0.9473165046940046 1103 1 0.4703380165398734 1103 2 0.4218184896409234 1103 3 0.4267512705845924 1103 4 0.4672875111381174 1103 5 0.49035946796200136 1103 6 0.5225207427678455 1103 7 -11.763628107303116 1103 8 -6.255039245374768 1103 9 -1.900497109148688 1103 10-11 0.050656328840084086 1103 12-13 0.5624094487737779 1103 14-15 0.8061798758967242 1103 16-17 0.7748523116785861 1103 18-19 0.8653109933859398 1103 20-21 0.79633471542639 1103 22-23 0.8095415572772922 1103 24-25 0.8813648662835263 1103 26-27 0.8435626870267825 1103 28-29 0.8204991857613564 1103 30-31 0.7889401737444004 1103 32-33 0.8833780183150068 1103 34-35 0.8689039914567047 1103 36-37 0.8546223420794661 1103 38-39 0.8945855482326905 1103 40-41 0.93849318082097 1103 42-43 0.9365209207292082 1103 44-45 0.8855554953451517 1103 46-47 0.9244888398883688 1103 48-49 0.8147962117499006 1103 50-51 0.8260852301047237 1103 52-53 0.8420985111695245 1103 54-55 0.8309631098582173 1103 56-57 0.8588359767863594 1103 58-59 0.7819886719440703 1103 60-61 0.7742525742864999 1103 62-63 0.6615655823857338 1103 64-65 0.605994843480012 1103 66-67 -9.775719260187312 1103 68-69 -7.731713791792512 1103 70-71 -6.306353399054647 1103 72-73 -1.2526282994152709 1103 74-75 0.008903784182237473 1103 76-77 0.37033402939516336 1103 78-79 3.378514601139088 1103 80-81 1.3839081049059807 1103 82-83 0.6573026711193322 1103 84-85 -4.987339367344138 1103 86-87 -5.494323060806085 1103 88-89 -1.1155174914494594 1103 90-91 0.23677101825165536 1103 92-93 0.6976250280301137 1103 94-95 0.7080000344245612 1103 96-97 0.801551941673317 1103 98-99 0.8209063973712389 1103 100 0.9004430696973493 1104 1 0.3519426992117225 1104 2 0.4084656287615793 1104 3 0.4205091549983031 1104 4 0.44392826875032654 1104 5 0.4393141277321959 1104 6 0.45758541888435644 1104 7 -11.920971631542184 1104 8 -6.375324677548036 1104 9 -2.040887056801566 1104 10-11 -0.0650763676173085 1104 12-13 0.522301449805525 1104 14-15 0.736147280093391 1104 16-17 0.7769143741828373 1104 18-19 0.7444191418851389 1104 20-21 0.7611942278770769 1104 22-23 0.7433462443854353 1104 24-25 0.845124784039065 1104 26-27 0.8173728748918734 1104 28-29 0.8047941154043841 1104 30-31 0.7650362461783757 1104 32-33 0.7459703824542672 1104 34-35 0.7484465488982934 1104 36-37 0.8197761022160535 1104 38-39 0.8432640097246988 1104 40-41 0.9118363500622166 1104 42-43 0.8450953389116052 1104 44-45 0.8613228991983917 1104 46-47 0.8731921918429322 1104 48-49 0.8104486959180335 1104 50-51 0.8623391434331111 1104 52-53 0.8374953084628629 1104 54-55 0.8725626077553699 1104 56-57 0.8754987909002523 1104 58-59 0.7872183545384956 1104 60-61 0.7869271435985823 1104 62-63 0.6785413326144152 1104 64-65 0.5473826497376635 1104 66-67 -9.840333740559096 1104 68-69 -7.851536137478327 1104 70-71 -6.334348249971047 1104 72-73 -1.4134291516358566 1104 74-75 -0.09942384480906696 1104 76-77 0.164631841219677 1104 78-79 3.194572326500822 1104 80-81 1.2548517974890956 1104 82-83 0.6160852255529008 1104 84-85 -5.0192094229619535 1104 86-87 -5.466314900215661 1104 88-89 -1.1382472313401664 1104 90-91 0.16455936026465778 1104 92-93 0.5320255188736418 1104 94-95 0.5831492625289378 1104 96-97 0.6397792793893657 1104 98-99 0.7387192896366344 1104 100 0.8509336070444498 1105 1 0.22015391321818 1105 2 0.24573513392420665 1105 3 0.2772151661884976 1105 4 0.29663212569627007 1105 5 0.30346661721439716 1105 6 0.3585999575587877 1105 7 -11.954279687569926 1105 8 -6.459069932992282 1105 9 -2.2742531526619842 1105 10-11 -0.2544509994793742 1105 12-13 0.28941080296842614 1105 14-15 0.48717582450447594 1105 16-17 0.5218781197186928 1105 18-19 0.491882866427396 1105 20-21 0.4794752718772841 1105 22-23 0.5330438291906248 1105 24-25 0.5784138970830597 1105 26-27 0.5381901156237987 1105 28-29 0.5237691181635427 1105 30-31 0.5451848576910336 1105 32-33 0.5635012947945413 1105 34-35 0.5658727218712443 1105 36-37 0.6042731613112693 1105 38-39 0.5229349416430225 1105 40-41 0.5114414640155189 1105 42-43 0.5020300867243677 1105 44-45 0.42437695429402567 1105 46-47 0.4118467054090047 1105 48-49 0.390513599161487 1105 50-51 0.49779123556626814 1105 52-53 0.4610429668056639 1105 54-55 0.46424328265793235 1105 56-57 0.4442554413758444 1105 58-59 0.4394812294693651 1105 60-61 0.37478921588378 1105 62-63 0.33310063476284313 1105 64-65 0.16978832320066317 1105 66-67 -10.02259649852557 1105 68-69 -8.093113088954297 1105 70-71 -6.558171209512164 1105 72-73 -1.61743994325089 1105 74-75 -0.4293720798163321 1105 76-77 -0.11033624099610151 1105 78-79 2.9568203469533962 1105 80-81 0.9662246730857653 1105 82-83 0.35582928496121724 1105 84-85 -5.200166164729762 1105 86-87 -5.715553139595592 1105 88-89 -1.3823495153549104 1105 90-91 -0.15096764456507117 1105 92-93 0.219554587525149 1105 94-95 0.2622271362241868 1105 96-97 0.30962758631473974 1105 98-99 0.3058818316088612 1105 100 0.38550775767299683 1106 1 0.29323688509340684 1106 2 0.3543271770187495 1106 3 0.35805119896453164 1106 4 0.3327781087991113 1106 5 0.30631213007690405 1106 6 0.3841291503291657 1106 7 -11.928584656498323 1106 8 -6.425239601944924 1106 9 -2.2459403876741177 1106 10-11 -0.21158504900545694 1106 12-13 0.34334914835802266 1106 14-15 0.5170527146890223 1106 16-17 0.5654505225358832 1106 18-19 0.5910940157147664 1106 20-21 0.5652492483589455 1106 22-23 0.586187850277021 1106 24-25 0.687432783269152 1106 26-27 0.6958418719998818 1106 28-29 0.6620539642479031 1106 30-31 0.6586123906561312 1106 32-33 0.6743274786991478 1106 34-35 0.7280775286894112 1106 36-37 0.7391153667458923 1106 38-39 0.7223293233255461 1106 40-41 0.7436208717931407 1106 42-43 0.7427575046434995 1106 44-45 0.6063679757378608 1106 46-47 0.7056139953466811 1106 48-49 0.6295933549790078 1106 50-51 0.6954073467999677 1106 52-53 0.6596845573879904 1106 54-55 0.6098937247976153 1106 56-57 0.6160570543180057 1106 58-59 0.5934790013875784 1106 60-61 0.578866444655155 1106 62-63 0.47547935666077024 1106 64-65 0.40741460008447206 1106 66-67 -9.904505085684093 1106 68-69 -7.945553383717925 1106 70-71 -6.451375599561668 1106 72-73 -1.5138125258202173 1106 74-75 -0.29661510048552486 1106 76-77 0.06651325077886128 1106 78-79 3.051489640799872 1106 80-81 1.0774157881696595 1106 82-83 0.41168759999178306 1106 84-85 -5.199586409486894 1106 86-87 -5.64131730886832 1106 88-89 -1.3243565466920089 1106 90-91 -0.060926336195908704 1106 92-93 0.3853972920477986 1106 94-95 0.44002345142025234 1106 96-97 0.5579622032840739 1106 98-99 0.5614480745535317 1106 100 0.7091480229568141 1107 1 0.1618520586853478 1107 2 0.19860633163524 1107 3 0.2067819233192516 1107 4 0.20115422641168834 1107 5 0.2379770350465904 1107 6 0.25053655601012537 1107 7 -11.99832489944314 1107 8 -6.504601879526749 1107 9 -2.3562175696766943 1107 10-11 -0.35201439189409456 1107 12-13 0.21384638955020563 1107 14-15 0.334569896461673 1107 16-17 0.35682670989468335 1107 18-19 0.38420357570085883 1107 20-21 0.3521819601495295 1107 22-23 0.43534700272182647 1107 24-25 0.4802432330796975 1107 26-27 0.42132416250831994 1107 28-29 0.41961613576008716 1107 30-31 0.4486786406160448 1107 32-33 0.47813300710047457 1107 34-35 0.3923467546438246 1107 36-37 0.500847133685653 1107 38-39 0.4810517268035639 1107 40-41 0.47715215181280257 1107 42-43 0.5007794145233007 1107 44-45 0.44726152609479897 1107 46-47 0.47657632929609406 1107 48-49 0.43972599643318233 1107 50-51 0.42725084727095464 1107 52-53 0.43171893705869735 1107 54-55 0.37390358277643543 1107 56-57 0.35593900964178715 1107 58-59 0.3393377338068433 1107 60-61 0.3193608996347237 1107 62-63 0.25562339540559265 1107 64-65 0.06456823943099721 1107 66-67 -9.968834563031756 1107 68-69 -8.077805100979134 1107 70-71 -6.579233574072926 1107 72-73 -1.745974793237302 1107 74-75 -0.4399494777045625 1107 76-77 -0.11740946609982572 1107 78-79 2.8806239075354085 1107 80-81 0.9237699802944981 1107 82-83 0.2337338072902817 1107 84-85 -5.254838661515752 1107 86-87 -5.755105664902324 1107 88-89 -1.5700128059832608 1107 90-91 -0.32434980215069587 1107 92-93 0.1384139664855688 1107 94-95 0.2134918732694011 1107 96-97 0.22663652130847112 1107 98-99 0.2730980928236306 1107 100 0.38106452284162273 1108 1 0.1252189953102345 1108 2 0.1668159295508005 1108 3 0.1648739900645495 1108 4 0.11274637462875603 1108 5 0.06409841100060021 1108 6 0.15483005782804327 1108 7 -12.034480702048228 1108 8 -6.574685725400464 1108 9 -2.5090654991076846 1108 10-11 -0.4678646758979639 1108 12-13 0.13700597830364103 1108 14-15 0.2880454178065577 1108 16-17 0.32205496214365326 1108 18-19 0.3111685499536847 1108 20-21 0.36313967243755485 1108 22-23 0.3905520961915201 1108 24-25 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-4.235773410530925 2216 50-51 -4.582482374314559 2216 52-53 -4.459621757774862 2216 54-55 -4.185123883151604 2216 56-57 -4.259265674063364 2216 58-59 -4.393299482124718 2216 60-61 -4.598998659920234 2216 62-63 -4.460293566187911 2216 64-65 -4.1612048224604195 2216 66-67 -0.8672047076157625 2216 68-69 -1.623040811840383 2216 70-71 -2.0260766050056525 2216 72-73 -3.732729837436569 2216 74-75 -4.36775436319197 2216 76-77 -4.051847694166593 2216 78-79 -1.4593266209325506 2216 80-81 -3.0739157917288047 2216 82-83 -3.7485628353121427 2216 84-85 -2.1798528332827125 2216 86-87 -2.0666934830339656 2216 88-89 -3.195997349352467 2216 90-91 -3.4840655619987118 2216 92-93 -3.611442853527013 2216 94-95 -3.518039766817939 2216 96-97 -3.6737106720654467 2216 98-99 -3.6606090790421817 2216 100 -4.487038694846401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 125992.0 3 24539.0 4 14272.0 5 13271.0 6 12917.0 7 15622.0 8 19004.0 9 21267.0 10 22717.0 11 25030.0 12 27610.0 13 30119.0 14 34688.0 15 37077.0 16 37602.0 17 38020.0 18 36251.0 19 35284.0 20 34169.0 21 33709.0 22 36634.0 23 43625.0 24 53908.0 25 66567.0 26 80840.0 27 96680.0 28 119148.0 29 148498.0 30 179913.0 31 221308.0 32 288809.0 33 395474.0 34 573003.0 35 714630.0 36 857461.0 37 1323915.0 38 1285656.0 39 345478.0 40 557.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14980858970836 20.776085500568335 14.127789009995745 22.946316899727567 2 15.959588820978327 20.63309008939914 39.78457793570185 23.622743153920688 3 18.36352982086627 25.083340271105843 30.45842302244706 26.094706885580827 4 12.857272116679603 17.351909203512296 38.760583076767816 31.03023560304029 5 12.982106491039861 37.430514332140525 35.308697662808044 14.278681514011573 6 30.38671585392183 38.1155183059496 17.94697686707917 13.550788973049405 7 26.807064759485634 32.40958869463597 22.64357978095007 18.13976676492833 8 24.370923045953134 36.13590685592157 21.150062425849228 18.34310767227607 9 24.09133180141941 17.65392040757762 22.305047713479272 35.949700077523694 10-11 23.285517684825486 27.430472273500172 29.075160508315594 20.208849533358748 12-13 23.895769176407097 24.914934072735214 28.931730962792905 22.257565788064777 14-15 21.468622979993746 27.48848119943292 26.16442813424877 24.878467686324562 16-17 20.888528812250804 30.544089749919994 26.822724018433863 21.744657419395338 18-19 20.707747711766043 29.434905526026117 28.866614805741037 20.990731956466803 20-21 22.05143079212773 28.89612981793508 28.184877966089672 20.86756142384752 22-23 21.671466836133753 28.84864595870257 28.226562466538457 21.253324738625217 24-25 21.450854902195935 28.801619110233556 28.345558395473645 21.401967592096867 26-27 21.17741688191948 28.664072233661997 28.464487251034914 21.69402363338361 28-29 21.291751971286253 28.702258413034258 28.55247786719891 21.453511748480576 30-31 21.76517922536267 29.267845440878542 27.922457297720975 21.04451803603781 32-33 21.091871761423974 28.981208261736775 28.52371527059284 21.403204706246413 34-35 21.240859220705588 28.507593466348407 28.55346257929638 21.698084733649626 36-37 21.787062212515103 28.938283786594553 27.936317853663812 21.338336147226535 38-39 21.590538200131746 28.990899598173726 28.165389963826065 21.25317223786847 40-41 21.30121489482904 28.68780972001525 28.442068169455663 21.568907215700047 42-43 21.20963775662249 28.587448050105056 28.449987997341548 21.752926195930904 44-45 21.459899112010085 28.741030959185444 28.33441258069594 21.46465734810854 46-47 21.29584425219675 29.04623235885663 28.166494929567264 21.491428459379353 48-49 21.087721660526213 28.61612196773165 28.495231436035496 21.800924935706636 50-51 21.146442509395193 28.416203864350543 28.483160598789294 21.954193027464978 52-53 21.099290459764646 28.458945906817558 28.460926834631945 21.98083679878585 54-55 21.245803738151388 28.889816446629357 28.255336167403694 21.60904364781556 56-57 21.005519615931746 28.606060735852417 28.250781805050128 22.137637843165702 58-59 20.846380309452 28.154899866347478 28.619889644817302 22.37883017938322 60-61 21.22164571643799 28.499190788257163 28.16012695218192 22.119036543122924 62-63 21.034672524626096 28.47933738169478 28.213566202568742 22.27242389111038 64-65 20.995585110252453 28.141637859916507 28.46714136541352 22.39563566441752 66-67 20.87059786739373 28.175256261654518 28.21393068976273 22.74021518118902 68-69 21.524771207789033 28.303355619094184 28.002706887327587 22.169166285789196 70-71 21.21268358053451 27.969713461510665 28.22174435170794 22.595858606246892 72-73 20.84832453965974 28.00785765525431 28.587096385172075 22.55672141991388 74-75 20.86271781368758 27.97191939461367 28.48471114689956 22.680651644799187 76-77 20.927415574029805 28.001401976835528 28.24317404664337 22.8280084024913 78-79 21.116794974504792 28.02813310483905 28.3580594496486 22.49701247100756 80-81 20.84654422486135 27.763903669285455 28.524201840180847 22.865350265672355 82-83 20.760900554815652 27.692408595961695 28.691183852120545 22.85550699710211 84-85 20.872770360574187 27.52205570156966 28.526701826234213 23.07847211162194 86-87 21.090397101176336 27.569704072282935 28.45976201007344 22.880136816467285 88-89 20.922397469416065 27.418422652115247 28.57288847074772 23.086291407720967 90-91 20.929469300615878 27.230876568348105 28.548484731872914 23.2911693991631 92-93 21.14240732816286 27.365452315502598 28.264279556199405 23.227860800135137 94-95 21.089280412818777 27.05026035413302 28.470322091699373 23.39013714134883 96-97 21.110575489018395 26.993539808622963 28.51783417447864 23.37805052788 98-99 21.268272239367274 26.93994134036935 28.40134570204163 23.39044071822175 100 21.423002176320367 26.965330797616932 28.221931939090872 23.389735086971832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2764.0 1 2321.0 2 2049.5 3 2056.0 4 1680.0 5 1384.5 6 1310.5 7 1258.5 8 1285.0 9 1354.5 10 1289.5 11 1180.0 12 1174.5 13 1315.0 14 1460.0 15 1628.0 16 2030.5 17 2702.5 18 3619.5 19 5048.0 20 7277.0 21 10479.5 22 15155.5 23 22045.5 24 30497.5 25 40907.5 26 55751.5 27 74285.0 28 97024.0 29 120688.0 30 140935.5 31 160418.5 32 178618.5 33 196356.5 34 214136.5 35 229666.5 36 242754.5 37 253435.0 38 261807.5 39 266815.0 40 269904.0 41 269118.5 42 264416.5 43 260689.0 44 256410.5 45 251720.5 46 247781.5 47 242900.0 48 237637.0 49 234538.5 50 229604.5 51 220310.5 52 209357.0 53 199354.5 54 191473.0 55 182634.0 56 168349.0 57 150355.0 58 133039.0 59 116716.0 60 99202.5 61 81525.0 62 66529.0 63 53390.0 64 42039.0 65 32735.0 66 25239.5 67 19232.5 68 14620.5 69 11078.0 70 8271.0 71 6205.0 72 4816.5 73 3650.5 74 2765.5 75 2031.0 76 1359.5 77 911.5 78 673.0 79 508.5 80 367.0 81 244.0 82 171.0 83 134.0 84 119.0 85 95.5 86 64.5 87 50.5 88 44.5 89 35.0 90 29.5 91 28.5 92 24.5 93 19.5 94 17.0 95 18.5 96 17.0 97 13.5 98 16.0 99 21.0 100 35.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01586077001160714 2 0.0021816924150987035 3 0.001151076979745328 4 0.0018470770140099453 5 0.001592769309182489 6 0.002984769377711723 7 11.833205197942409 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0076924065325492E-4 18-19 0.0 20-21 6.6923080217751635E-6 22-23 0.0 24-25 0.0 26-27 1.4053846845727845E-4 28-29 0.0 30-31 0.0 32-33 2.6769232087100654E-5 34-35 7.361538823952681E-5 36-37 2.6769232087100654E-5 38-39 6.6923080217751635E-6 40-41 0.0 42-43 3.346154010887582E-5 44-45 1.0707692834840262E-4 46-47 7.696154225041438E-4 48-49 5.621538738291138E-4 50-51 2.87769244936332E-4 52-53 2.3423078076213077E-4 54-55 4.149230973500602E-4 56-57 0.0012313846760066303 58-59 3.2792309306698303E-4 60-61 0.0013986923765510093 62-63 0.0023490001156430827 64-65 9.302308150267478E-4 66-67 5.916957291296359 68-69 5.557145350505617 70-71 0.0020880001027938516 72-73 0.0010506923594187007 74-75 0.0026769232087100656 76-77 0.0051463848687451005 78-79 5.530168656869842 80-81 0.004196077129653027 82-83 0.004202769437674803 84-85 5.9169706759124026 86-87 0.003125307846169002 88-89 0.0013786154524856838 90-91 0.0030316155338641496 92-93 0.003359538626931132 94-95 0.003828000188455394 96-97 0.0026836155167318408 98-99 0.004925538704026521 100 0.00531369256928948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7471264.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.72173261615536 #Duplication Level Percentage of deduplicated Percentage of total 1 92.96107944465189 83.40629113642234 2 5.23640217866718 9.396381522900601 3 0.9954663881133076 2.6794490730801637 4 0.3401791093403174 1.2208583635933536 5 0.17241232352374605 0.7734566195463808 6 0.09446804380217509 0.5085501940074003 7 0.05507141905052969 0.34587721943907085 8 0.03540626423034744 0.25413690977697434 9 0.02555960002671883 0.20639264394358303 >10 0.0826607733524861 1.1186424361984248 >50 0.0010857400539665944 0.061581853884307064 >100 1.9328928662225897E-4 0.019939392103761108 >500 1.5425900740192007E-5 0.008442635103606648 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.3384616043550329E-5 0.0 0.0 0.0 4 0.0 1.3384616043550329E-5 0.0 1.3384616043550329E-5 0.0 5 0.0 1.3384616043550329E-5 0.0 1.3384616043550329E-5 0.0 6 0.0 1.3384616043550329E-5 0.0 2.6769232087100657E-5 0.0 7 0.0 1.3384616043550329E-5 0.0 4.015384813065098E-5 0.0 8 0.0 1.3384616043550329E-5 0.0 4.015384813065098E-5 0.0 9 0.0 1.3384616043550329E-5 0.0 4.015384813065098E-5 0.0 10-11 0.0 1.3384616043550329E-5 0.0 4.015384813065098E-5 0.0 12-13 0.0 4.015384813065099E-5 0.0 6.023077219597648E-5 0.0 14-15 0.0 8.700000428307714E-5 0.0 8.030769626130197E-5 6.692308021775164E-6 16-17 0.0 1.3384616043550328E-4 0.0 9.369231230485229E-5 1.3384616043550329E-5 18-19 0.0 1.539230845008288E-4 0.0 1.539230845008288E-4 1.3384616043550329E-5 20-21 0.0 1.6061539252260393E-4 0.0 3.011538609798824E-4 1.3384616043550329E-5 22-23 0.0 1.6061539252260393E-4 0.0 6.424615700904158E-4 1.3384616043550329E-5 24-25 0.0 1.8738462460970458E-4 0.0 0.0013384616043550328 1.3384616043550329E-5 26-27 0.0 2.1415385669680526E-4 0.0 0.003098538614081901 3.346154010887582E-5 28-29 0.0 2.1415385669680526E-4 0.0 0.009543231239051384 4.015384813065098E-5 30-31 0.0 2.1415385669680526E-4 0.0 0.029111539894721962 4.015384813065098E-5 32-33 0.0 2.1415385669680526E-4 0.0 0.07745008073600397 4.015384813065098E-5 34-35 0.0 2.2753847274035557E-4 0.0 0.16131139255686855 4.015384813065098E-5 36-37 0.0 2.4761539680568105E-4 0.0 0.299587860902787 4.015384813065098E-5 38-39 0.0 2.7438462889278173E-4 0.0 0.48408140844708475 6.692308021775164E-5 40-41 0.0 3.2123078504520786E-4 0.0 0.6907733416996107 6.692308021775164E-5 42-43 0.0 3.346154010887582E-4 0.0 0.9103078140459231 7.361538823952681E-5 44-45 0.0 3.346154010887582E-4 0.0 1.1812592889235343 8.030769626130197E-5 46-47 0.0 3.346154010887582E-4 0.0 1.5094166127712794 8.030769626130197E-5 48-49 0.0 3.346154010887582E-4 0.0 1.8620209378225692 8.030769626130197E-5 50-51 0.0 3.346154010887582E-4 0.0 2.2201129554517145 8.030769626130197E-5 52-53 0.0 3.346154010887582E-4 0.0 2.5710709727296477 8.030769626130197E-5 54-55 0.0 3.346154010887582E-4 0.0 2.953676914642556 8.030769626130197E-5 56-57 0.0 3.4130770911053337E-4 0.0 3.3630520886425646 8.030769626130197E-5 58-59 0.0 3.613846331758589E-4 0.0 3.800755802498747 8.030769626130197E-5 60-61 0.0 4.0153848130650984E-4 0.0 4.245145132068684 8.030769626130197E-5 62-63 0.0 4.0153848130650984E-4 0.0 4.681383230468098 8.030769626130197E-5 64-65 0.0 4.08230789328285E-4 0.0 5.154957715320995 8.030769626130197E-5 66-67 0.0 4.283077133936105E-4 0.0 5.671978663851258 8.030769626130197E-5 68-69 0.0 4.283077133936105E-4 0.0 6.169344303721566 8.030769626130197E-5 70-71 0.0 4.4169232943716086E-4 0.0 6.737046100900731 8.030769626130197E-5 72-73 0.0 4.7515386954603665E-4 0.0 7.344272669256501 8.030769626130197E-5 74-75 0.0 4.885384855895869E-4 0.0 7.878713695567443 8.030769626130197E-5 76-77 0.0 5.086154096549124E-4 0.0 8.417136645151341 8.030769626130197E-5 78-79 0.0 5.153077176766876E-4 0.0 9.027582213665585 8.030769626130197E-5 80-81 0.0 5.353846417420131E-4 0.0 9.730535555964828 8.030769626130197E-5 82-83 0.0 5.420769497637883E-4 0.0 10.432846436693978 8.030769626130197E-5 84-85 0.0 5.822307978944393E-4 0.0 11.112771547090292 8.030769626130197E-5 86-87 0.0 6.023077219597648E-4 0.0 11.889761893034432 8.030769626130197E-5 88 0.0 6.023077219597648E-4 0.0 12.522338924176685 8.030769626130197E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6425 0.0 47.965515 1 GTATCAA 10570 0.0 35.171494 1 TCAACGC 10735 0.0 33.19336 4 ATCAACG 10810 0.0 32.915634 3 CAACGCA 11700 0.0 30.455616 5 TATCAAC 12625 0.0 28.670927 2 AACGCAG 12765 0.0 27.914452 6 ACGCAGA 14815 0.0 23.913229 7 GTACATG 16975 0.0 23.682858 1 CGCAGAG 14905 0.0 23.321892 8 TACATGG 17340 0.0 21.85062 2 ACATGGG 17955 0.0 20.04565 3 GCAGAGT 17345 0.0 19.128952 9 GTCTCGC 2000 0.0 18.45994 1 GTGGTAT 3945 0.0 18.197378 1 GTCGTAC 325 8.011739E-8 17.3555 1 CAGAGTA 16845 0.0 17.093771 10-11 AGAGTAC 15020 0.0 16.477207 10-11 TCTGTCG 2200 0.0 16.437536 8 CATGGGG 12305 0.0 16.374928 4 >>END_MODULE