FastQCFastQC Report
Wed 25 May 2016
SRR2049492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7471264
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193600.25912616660313437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192730.25796170500734544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108710.1455041610094356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130800.057.910221
GTATCAA240150.040.5979771
GTGGTAT52350.036.1506651
TGGTATC53500.033.945212
ATCAACG302700.031.8474353
TCAACGC304800.031.6743224
TATCAAC312550.030.9942992
CAACGCA317400.030.4139755
AACGCAG334350.028.9701376
ACGCAGA380600.025.326247
CGCAGAG379350.025.2981958
GCAGAGT407300.023.1236929
GTACATG177200.022.6616861
TACATGG177900.021.4481532
GAGTACT310350.020.92811812-13
CAGAGTA400250.020.62484610-11
AGAGTAC381200.020.44115810-11
GCGGTAT4700.020.0326791
ACATGGG182950.019.9303193
AGTACTT323350.018.93849212-13
Produced by FastQC (version 0.11.5)