##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049492_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7471264
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643862136313214	31.0	31.0	34.0	30.0	34.0
2	31.694170491097623	31.0	31.0	34.0	30.0	34.0
3	31.72139332782244	31.0	31.0	34.0	30.0	34.0
4	35.450806717578175	37.0	35.0	37.0	33.0	37.0
5	35.60569148674173	37.0	35.0	37.0	33.0	37.0
6	35.70247524381416	37.0	35.0	37.0	33.0	37.0
7	35.717033021453936	37.0	35.0	37.0	33.0	37.0
8	35.726614265002546	37.0	35.0	37.0	33.0	37.0
9	37.43881410160316	39.0	37.0	39.0	34.0	39.0
10-11	37.411980489512885	39.0	37.0	39.0	34.0	39.0
12-13	37.402526466739765	39.0	37.0	39.0	34.0	39.0
14-15	38.66151892102863	40.0	38.0	41.0	34.0	41.0
16-17	38.67739822873345	40.0	38.0	41.0	34.0	41.0
18-19	38.74865129916437	40.0	38.0	41.0	34.0	41.0
20-21	38.77827285985343	40.0	38.0	41.0	34.0	41.0
22-23	38.750003413077096	40.0	38.0	41.0	34.0	41.0
24-25	38.71578256370006	40.0	38.0	41.0	34.0	41.0
26-27	38.62078605440793	40.0	38.0	41.0	34.0	41.0
28-29	38.530591209198334	40.0	38.0	41.0	34.0	41.0
30-31	38.41007873634234	40.0	38.0	41.0	34.0	41.0
32-33	38.25841919118372	40.0	38.0	41.0	34.0	41.0
34-35	38.1461392610407	40.0	38.0	41.0	34.0	41.0
36-37	37.99574221979039	40.0	37.5	41.0	33.0	41.0
38-39	37.891223225414066	40.0	37.0	41.0	33.0	41.0
40-41	37.75802822654908	40.0	37.0	41.0	33.0	41.0
42-43	37.61280641401508	40.0	37.0	41.0	33.0	41.0
44-45	37.4315009481662	40.0	37.0	41.0	32.5	41.0
46-47	37.19454077650047	40.0	37.0	41.0	32.0	41.0
48-49	36.95918876645237	40.0	37.0	41.0	31.0	41.0
50-51	36.751014620819184	40.0	36.0	41.0	31.0	41.0
52-53	36.646467451826084	40.0	36.0	41.0	31.0	41.0
54-55	36.51704323391597	39.5	36.0	41.0	30.5	41.0
56-57	36.34764438788403	39.0	35.0	41.0	30.0	41.0
58-59	36.182934440544464	39.0	35.0	41.0	30.0	41.0
60-61	35.99781857527722	39.0	35.0	41.0	30.0	41.0
62-63	35.79641577382355	39.0	35.0	41.0	29.0	41.0
64-65	35.530449399191355	38.0	35.0	40.0	29.0	41.0
66-67	35.27531104509224	37.5	35.0	40.0	29.0	41.0
68-69	34.96285882817151	37.0	34.0	40.0	28.5	41.0
70-71	34.66976879681938	36.5	34.0	39.5	28.0	41.0
72-73	34.36222277783251	36.0	34.0	39.0	28.0	41.0
74-75	34.01551082922515	36.0	34.0	39.0	28.0	40.5
76-77	33.7235210133118	35.0	34.0	38.5	28.0	40.0
78-79	33.390451401529916	35.0	34.0	37.0	27.0	39.0
80-81	33.07554130064203	35.0	33.5	37.0	27.0	39.0
82-83	32.78497795018353	35.0	33.0	36.0	26.0	39.0
84-85	32.505069155634175	35.0	33.0	36.0	26.0	37.0
86-87	32.23784576478626	35.0	33.0	36.0	26.0	37.0
88-89	32.00702297496113	35.0	33.0	35.0	26.0	37.0
90-91	31.84362411768611	35.0	33.0	35.0	26.0	36.0
92-93	31.676903801016802	35.0	33.0	35.0	25.0	36.0
94-95	31.48554059928815	35.0	33.0	35.0	25.0	36.0
96-97	31.30484426731541	35.0	33.0	35.0	25.0	35.5
98-99	31.11119463855112	35.0	33.0	35.0	24.0	35.0
100	30.992337708853547	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42787782088621995
1101	2	0.49489340920672475
1101	3	0.6010683766268343
1101	4	0.5060709763190445
1101	5	0.39324448769352927
1101	6	0.34818439406503643
1101	7	0.3666174179423365
1101	8	0.3772422112624838
1101	9	0.40948731213254774
1101	10-11	0.45028450318137203
1101	12-13	0.43276588969678187
1101	14-15	0.570661270225223
1101	16-17	0.5682746462930055
1101	18-19	0.5816476629443557
1101	20-21	0.5403071314656174
1101	22-23	0.5382029258780037
1101	24-25	0.5800638952927244
1101	26-27	0.5665060926939915
1101	28-29	0.5607870641994808
1101	30-31	0.5668184450833138
1101	32-33	0.6062685436026172
1101	34-35	0.6095036389151716
1101	36-37	0.5861248320507713
1101	38-39	0.5935456940924055
1101	40-41	0.6116927321707522
1101	42-43	0.5708360680992541
1101	44-45	0.6041036096747945
1101	46-47	0.667178422227849
1101	48-49	0.6735995829520931
1101	50-51	0.687878706301781
1101	52-53	0.7244269892318229
1101	54-55	0.6521021932222482
1101	56-57	0.602765619844746
1101	58-59	0.6155239114130424
1101	60-61	0.6614666540968273
1101	62-63	0.649495992996286
1101	64-65	0.633653288082769
1101	66-67	0.6940021127856326
1101	68-69	0.6298210417557115
1101	70-71	0.6067549701389936
1101	72-73	0.6319844266258627
1101	74-75	0.5854803432138809
1101	76-77	0.5626655127433295
1101	78-79	0.5397326967713383
1101	80-81	0.5460887971812909
1101	82-83	0.5606262496305519
1101	84-85	0.5885773806905661
1101	86-87	0.6459197318345815
1101	88-89	0.6297213982786616
1101	90-91	0.6460448302080373
1101	92-93	0.5888039291946825
1101	94-95	0.5890468057710265
1101	96-97	0.6345173305702687
1101	98-99	0.7067982155307995
1101	100	0.7342979099722307
1102	1	0.3590121739478498
1102	2	0.43337313685319856
1102	3	0.5122044867132018
1102	4	0.4329967063301936
1102	5	0.33609275097655456
1102	6	0.3146700935214142
1102	7	0.31666911967005973
1102	8	0.3396900466196726
1102	9	0.4324483931703256
1102	10-11	0.3717556523036336
1102	12-13	0.3961304373711556
1102	14-15	0.4687784103645427
1102	16-17	0.511722601219482
1102	18-19	0.5478848293932472
1102	20-21	0.49720322670067674
1102	22-23	0.4879088273334631
1102	24-25	0.4763628129939974
1102	26-27	0.43823470470970705
1102	28-29	0.4533358748955365
1102	30-31	0.5637796176453946
1102	32-33	0.5779629182671044
1102	34-35	0.5771495278208221
1102	36-37	0.5957665679652919
1102	38-39	0.5879976616122562
1102	40-41	0.5779313116719891
1102	42-43	0.5883889483428959
1102	44-45	0.6011919526280494
1102	46-47	0.6578803518826462
1102	48-49	0.5963655071987404
1102	50-51	0.6475705839687862
1102	52-53	0.5674171065510336
1102	54-55	0.543286587350984
1102	56-57	0.6208084965203184
1102	58-59	0.6036809596944508
1102	60-61	0.5967223536996968
1102	62-63	0.6082106995828767
1102	64-65	0.5503415704173662
1102	66-67	0.6488258478995874
1102	68-69	0.5791895477916214
1102	70-71	0.6016921547760603
1102	72-73	0.6400865750142515
1102	74-75	0.5744584483780812
1102	76-77	0.5642871739804036
1102	78-79	0.5277504621668783
1102	80-81	0.5033517717999771
1102	82-83	0.4877469679036466
1102	84-85	0.5125281601445124
1102	86-87	0.5172747146569208
1102	88-89	0.5251544735477225
1102	90-91	0.5440429239269484
1102	92-93	0.5666450600202992
1102	94-95	0.5464535444232723
1102	96-97	0.526531048154169
1102	98-99	0.6327770276100892
1102	100	0.7033490297557279
1103	1	0.40882781213785435
1103	2	0.41570424209484
1103	3	0.5312335309028953
1103	4	0.45558883796489624
1103	5	0.29508638583254054
1103	6	0.34602614886991034
1103	7	0.3264931914476037
1103	8	0.31809723643898025
1103	9	0.3542438747059862
1103	10-11	0.3396053060460318
1103	12-13	0.3188453129891684
1103	14-15	0.44255922872910247
1103	16-17	0.424076137771209
1103	18-19	0.44382459741505187
1103	20-21	0.4243858337443811
1103	22-23	0.4184254779464851
1103	24-25	0.44523695839190935
1103	26-27	0.4487924349756227
1103	28-29	0.47006406849190085
1103	30-31	0.49978389277850965
1103	32-33	0.4418397498775093
1103	34-35	0.4814862849543644
1103	36-37	0.5603893498154591
1103	38-39	0.5421079137881719
1103	40-41	0.5650965871396352
1103	42-43	0.5735908737228357
1103	44-45	0.5923101933681352
1103	46-47	0.610430320124479
1103	48-49	0.6234468632644194
1103	50-51	0.6058987826553803
1103	52-53	0.5973370019302564
1103	54-55	0.5872401013106696
1103	56-57	0.6490953986407533
1103	58-59	0.5623731305677282
1103	60-61	0.5774347282458479
1103	62-63	0.6097960132413149
1103	64-65	0.6029522371868268
1103	66-67	0.618143930040965
1103	68-69	0.6333775912600501
1103	70-71	0.6253221316597148
1103	72-73	0.634813640909563
1103	74-75	0.6288060243186919
1103	76-77	0.6207283760467561
1103	78-79	0.5871478862206914
1103	80-81	0.5355294202065508
1103	82-83	0.5832828021187879
1103	84-85	0.600852818393534
1103	86-87	0.5814815932201611
1103	88-89	0.5889202290423512
1103	90-91	0.5676413388983725
1103	92-93	0.5820431373755284
1103	94-95	0.6067434446568072
1103	96-97	0.6161702048896132
1103	98-99	0.6470933606042557
1103	100	0.5921449227655131
1104	1	0.35953139051761696
1104	2	0.34461340371466775
1104	3	0.4920003911177062
1104	4	0.3595236174729166
1104	5	0.26031662464980343
1104	6	0.2862417982066461
1104	7	0.23525040888787174
1104	8	0.24983787341261632
1104	9	0.2902391695029536
1104	10-11	0.3044757445825823
1104	12-13	0.3140235140780874
1104	14-15	0.40005123090873695
1104	16-17	0.41796137949578593
1104	18-19	0.3986996393346445
1104	20-21	0.38770227472954844
1104	22-23	0.4048898844556561
1104	24-25	0.382589988913729
1104	26-27	0.4289524193744185
1104	28-29	0.3871477121523341
1104	30-31	0.4631254241653764
1104	32-33	0.4181218911623219
1104	34-35	0.4410641580772392
1104	36-37	0.4157758603577264
1104	38-39	0.47058622616635404
1104	40-41	0.5099012435042027
1104	42-43	0.4597409962921901
1104	44-45	0.4991821141044852
1104	46-47	0.5165225213447044
1104	48-49	0.49265335523963927
1104	50-51	0.5395752186070979
1104	52-53	0.5502764291293971
1104	54-55	0.5180427610594762
1104	56-57	0.4949965063435684
1104	58-59	0.5239437500397912
1104	60-61	0.5312095442898794
1104	62-63	0.522874283980407
1104	64-65	0.5007489400273499
1104	66-67	0.5642617709858655
1104	68-69	0.47874453215147383
1104	70-71	0.4499475196131968
1104	72-73	0.5039680389382539
1104	74-75	0.5221039358069817
1104	76-77	0.4881250581437868
1104	78-79	0.4729757853062466
1104	80-81	0.502384536959994
1104	82-83	0.5182930751877421
1104	84-85	0.5233526172978884
1104	86-87	0.5520473490824997
1104	88-89	0.5516749429780177
1104	90-91	0.5251088034492248
1104	92-93	0.5294866884997447
1104	94-95	0.585644631841312
1104	96-97	0.5871383480691819
1104	98-99	0.6579252829749258
1104	100	0.6535636935583504
1105	1	0.2936507595816735
1105	2	0.2975899301600222
1105	3	0.41210077506083564
1105	4	0.34268985417046594
1105	5	0.2338879795293778
1105	6	0.252302449344441
1105	7	0.23752484155057374
1105	8	0.23186133797000252
1105	9	0.2709978365647032
1105	10-11	0.2278014292441739
1105	12-13	0.24346955195416342
1105	14-15	0.3514485093271844
1105	16-17	0.3013041237144449
1105	18-19	0.32507543679189155
1105	20-21	0.3204628661555162
1105	22-23	0.3102233671935082
1105	24-25	0.30433048735600465
1105	26-27	0.32612953990892635
1105	28-29	0.3259769187918309
1105	30-31	0.3315531872542934
1105	32-33	0.3335713283536421
1105	34-35	0.28608131360509503
1105	36-37	0.36774620808387937
1105	38-39	0.36801276202884026
1105	40-41	0.3640437287816809
1105	42-43	0.3789181187761059
1105	44-45	0.40106293533249726
1105	46-47	0.3677432510045904
1105	48-49	0.4027626592175082
1105	50-51	0.4128118903642175
1105	52-53	0.3654832101852463
1105	54-55	0.3304710866271705
1105	56-57	0.34298809270467956
1105	58-59	0.3560025400940958
1105	60-61	0.37028267792800307
1105	62-63	0.38872177875300196
1105	64-65	0.36674012106183795
1105	66-67	0.39007209057458425
1105	68-69	0.38024839437581903
1105	70-71	0.38221957100075343
1105	72-73	0.38428595266532284
1105	74-75	0.35329204589626784
1105	76-77	0.3438330631270645
1105	78-79	0.3207777993731682
1105	80-81	0.2851670892010887
1105	82-83	0.28737196632327766
1105	84-85	0.2692995235596243
1105	86-87	0.3125978525092563
1105	88-89	0.26229015920620213
1105	90-91	0.22121120862709276
1105	92-93	0.2621734086621288
1105	94-95	0.2740186595372833
1105	96-97	0.3174552908418029
1105	98-99	0.34881800023179466
1105	100	0.3447895194169597
1106	1	0.2972624530100312
1106	2	0.3070240801358288
1106	3	0.4252674540825865
1106	4	0.3672503650733958
1106	5	0.27519313062346384
1106	6	0.2660670498958808
1106	7	0.24303786783414694
1106	8	0.2663847606277372
1106	9	0.2989867905428696
1106	10-11	0.2724492202122022
1106	12-13	0.27205612217645125
1106	14-15	0.3484073299681114
1106	16-17	0.3511695789792597
1106	18-19	0.33476799124334633
1106	20-21	0.3348926983159828
1106	22-23	0.32897260218380353
1106	24-25	0.34945734320490374
1106	26-27	0.38618991677353876
1106	28-29	0.3920822227729346
1106	30-31	0.40381869777220203
1106	32-33	0.43697334447386993
1106	34-35	0.40075461279816693
1106	36-37	0.41654135012139903
1106	38-39	0.40324404493502897
1106	40-41	0.4108196438625953
1106	42-43	0.3887469060123294
1106	44-45	0.38744496882455337
1106	46-47	0.39287855715788567
1106	48-49	0.3558410905985028
1106	50-51	0.41099599436761025
1106	52-53	0.3871554395140393
1106	54-55	0.30936500398831157
1106	56-57	0.35691771985690934
1106	58-59	0.34859888031638775
1106	60-61	0.3480080878582754
1106	62-63	0.35833383694372856
1106	64-65	0.3037511002800102
1106	66-67	0.3263542561798616
1106	68-69	0.3629048413747995
1106	70-71	0.37485581025039494
1106	72-73	0.40712022814581417
1106	74-75	0.3579952645111888
1106	76-77	0.3606317352713546
1106	78-79	0.4056986320025473
1106	80-81	0.3040549453867385
1106	82-83	0.3268558304368696
1106	84-85	0.2980653035908105
1106	86-87	0.3740959986716561
1106	88-89	0.3931058348225491
1106	90-91	0.4216947773679145
1106	92-93	0.44680307787911744
1106	94-95	0.4502888737514894
1106	96-97	0.4927500925397936
1106	98-99	0.5084969317639256
1106	100	0.5274602034979772
1107	1	0.17563220283591363
1107	2	0.22895984169778671
1107	3	0.30246984627590123
1107	4	0.21223724734330318
1107	5	0.21332650467169856
1107	6	0.19629631133513925
1107	7	0.18703654669777592
1107	8	0.22918938864968652
1107	9	0.20161943559833162
1107	10-11	0.21204529294747942
1107	12-13	0.1966605018727563
1107	14-15	0.2664895078840246
1107	16-17	0.21550622674759978
1107	18-19	0.24856865740857614
1107	20-21	0.2820014544932974
1107	22-23	0.28734390469176674
1107	24-25	0.28810470229365137
1107	26-27	0.28556171267972985
1107	28-29	0.3015370364027632
1107	30-31	0.2784821988247188
1107	32-33	0.28388039813011545
1107	34-35	0.2814829373346157
1107	36-37	0.3470772809567606
1107	38-39	0.3067292176253176
1107	40-41	0.32758700340279745
1107	42-43	0.295362868700181
1107	44-45	0.33538117644346244
1107	46-47	0.3686432939912052
1107	48-49	0.35803637635529384
1107	50-51	0.43577921711045065
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2214	3	-0.26063456273335817
2214	4	-0.25194810129328715
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2214	92-93	-0.38866302928966334
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2214	96-97	-0.36410173583822925
2214	98-99	-0.3159551019072282
2214	100	-0.33501953989694755
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2215	2	-0.562015526455454
2215	3	-1.2713698196634198
2215	4	-0.610089676900003
2215	5	-0.4948455524213742
2215	6	-0.48665365908120606
2215	7	-0.4451621972251658
2215	8	-0.5053720670801383
2215	9	-0.733542136296812
2215	10-11	-0.5748385318949332
2215	12-13	-0.5576946816214701
2215	14-15	-0.7116707773808173
2215	16-17	-0.7125762072169053
2215	18-19	-0.7109994438275393
2215	20-21	-0.6833085182554441
2215	22-23	-0.7222011995197164
2215	24-25	-0.7574999356957761
2215	26-27	-0.7293403565855172
2215	28-29	-0.6474145918340852
2215	30-31	-0.6798571885705655
2215	32-33	-0.7277432292194206
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2215	64-65	-0.885168990042267
2215	66-67	-0.7374541956833269
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2215	70-71	-0.8421893182346452
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2215	90-91	-0.6248117325971982
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2215	94-95	-0.9221625065604044
2215	96-97	-1.0109430569344227
2215	98-99	-1.0015744101719548
2215	100	-1.0829533998609833
2216	1	-1.2433223391124493
2216	2	-1.6922229595902003
2216	3	-1.4117090650119586
2216	4	-1.8343418005005887
2216	5	-1.1169295414060016
2216	6	-1.244007875027414
2216	7	-0.962241589651633
2216	8	-0.9376298807434722
2216	9	-1.035374695117703
2216	10-11	-1.2726277481467392
2216	12-13	-1.1092001265435982
2216	14-15	-1.4072508432393107
2216	16-17	-1.3388755924340572
2216	18-19	-1.4292257668821904
2216	20-21	-1.2462093753091636
2216	22-23	-1.3197903790627166
2216	24-25	-1.3501985579277687
2216	26-27	-1.447750829983029
2216	28-29	-1.6961195626247445
2216	30-31	-1.7572371683216943
2216	32-33	-1.5803998757464583
2216	34-35	-1.589392932737141
2216	36-37	-1.6173892569959492
2216	38-39	-1.7106366524716776
2216	40-41	-1.6705058700740025
2216	42-43	-2.1740861430556606
2216	44-45	-1.9355539473328918
2216	46-47	-1.8542920399285236
2216	48-49	-1.772875977518808
2216	50-51	-1.9643570259151488
2216	52-53	-1.8355860658820973
2216	54-55	-1.9472617617506387
2216	56-57	-2.0283917014541473
2216	58-59	-1.8985897353559267
2216	60-61	-2.157267980557961
2216	62-63	-2.163552733813056
2216	64-65	-2.1108867843836876
2216	66-67	-1.9401617002773506
2216	68-69	-2.1116284031574324
2216	70-71	-1.9269849044852805
2216	72-73	-1.8096950037308872
2216	74-75	-1.9067340851530474
2216	76-77	-2.011990592932978
2216	78-79	-2.062061234086471
2216	80-81	-1.8540183770442695
2216	82-83	-1.9697848434842413
2216	84-85	-2.0706968773547985
2216	86-87	-2.1679791225065337
2216	88-89	-1.9992920538757417
2216	90-91	-1.8484623808368177
2216	92-93	-1.9979604990244404
2216	94-95	-2.14172945491163
2216	96-97	-2.360248469449047
2216	98-99	-2.2767204559272862
2216	100	-2.3800962784426396
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	486.0
3	44.0
4	71.0
5	129.0
6	449.0
7	1178.0
8	2644.0
9	4822.0
10	7112.0
11	10191.0
12	14686.0
13	20398.0
14	24208.0
15	30491.0
16	37473.0
17	50663.0
18	49274.0
19	26791.0
20	20205.0
21	19510.0
22	20306.0
23	22675.0
24	26908.0
25	32978.0
26	42451.0
27	55163.0
28	71719.0
29	94303.0
30	122879.0
31	157556.0
32	203318.0
33	264246.0
34	358651.0
35	514028.0
36	835404.0
37	1510392.0
38	2032383.0
39	782509.0
40	2570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19821752189795	21.169097034542276	14.63702244623879	22.995662997320977
2	15.94120243675031	21.26731735548827	38.9433672651287	23.848112942632717
3	18.197871203587507	25.317242169464233	30.868123519661467	25.6167631072868
4	12.987345205293686	17.906404528379152	37.97287978789236	31.133370478434802
5	12.473543197752525	37.347026377898636	35.415259864590965	14.764170559757876
6	29.977063808815817	38.50805269648818	17.415992318196285	14.09889117649972
7	25.949825892914507	32.96268208431666	22.67890680880772	18.408585213961118
8	24.568332747979458	35.730272682105735	20.813800716987114	18.887593852927697
9	24.16047996168787	16.83829092885634	22.408259487050582	36.59296962240521
10-11	23.354736226694705	27.142689643947797	28.849643112597818	20.65293101675968
12-13	24.426004010299195	24.586003731899144	29.148271182529644	21.83972107527201
14-15	21.64470574488505	26.82091188458284	27.082668145756067	24.451714224776037
16-17	20.355879540597147	29.97166878322062	27.769210136330347	21.90324153985189
18-19	20.08351598872694	28.070611612707037	31.03779360493753	20.808078793628496
20-21	21.381669821866822	27.494751892049322	30.6922162033091	20.431362082774747
22-23	20.931739261254855	27.517191200846337	30.81406974776959	20.73699979012922
24-25	20.755075714096037	27.490408584143193	30.89759644418936	20.85691925757141
26-27	20.56611170479319	27.30424195959345	31.019329527105455	21.110316808507903
28-29	20.671313143278187	27.319341703415862	31.09980555469113	20.909539598614824
30-31	21.10619552532529	27.856333755262007	30.494507811078925	20.542962908333777
32-33	20.55246077186275	27.574807125996827	31.037501220509757	20.835230881630665
34-35	20.655206401487085	27.173741953168832	31.01874060399954	21.152311041344543
36-37	21.24643835367081	27.59166989682067	30.291808722058278	20.870083027450242
38-39	21.077905960758446	27.606874820646144	30.501010270818966	20.814208947776443
40-41	20.866777027287483	27.391134886948176	30.607009737575865	21.135078348188472
42-43	20.82777425613658	27.36229773168235	30.418012266732912	21.39191574544816
44-45	21.129095424817006	27.56443889548007	30.193699486459053	21.112766193243875
46-47	21.1064419621633	27.967884684572788	29.655022229170324	21.270651124093593
48-49	20.99822064914317	27.637913745251137	29.723022770979586	21.64084283462611
50-51	21.115369501064343	27.561213203013573	29.48347160534014	21.839945690581942
52-53	21.0248761119939	27.69088336324349	29.39695344723463	21.88728707752798
54-55	21.14014442536096	28.104548306685455	29.185202129117645	21.570105138835945
56-57	20.836280179632254	27.77861282910094	29.211984745820786	22.173122245446017
58-59	20.664955218366927	27.339061509746355	29.620324831638058	22.37565844024866
60-61	20.988704187136207	27.671602823832753	29.173209513142623	22.16648347588842
62-63	20.787754254166362	27.639633668412735	29.25515682486926	22.317455252551643
64-65	20.65222163210937	27.348779269478364	29.495276836690554	22.503722261721713
66-67	20.60688793756987	27.424750350141558	29.179373128830676	22.7889885834579
68-69	21.123621116855194	27.58299666562445	28.898276114992054	22.3951061025283
70-71	20.99520241822535	27.25949049585184	28.970506195471074	22.774800890451736
72-73	20.64101870848092	27.349756346449546	29.209488514928665	22.79973643014087
74-75	20.7098557887929	27.31931972956651	28.990830734933205	22.979993746707382
76-77	20.746362329051685	27.402766118290025	28.696991566621122	23.15387998603717
78-79	20.855872215854486	27.44095687799728	28.75439074463455	22.948780161513685
80-81	20.61628027516437	27.20731160749881	28.794858580101156	23.381549537235667
82-83	20.42526026314386	27.168106644730095	28.927018974703856	23.479614117422194
84-85	20.508490932725707	27.022422176488476	28.737111953211663	23.73197493757415
86-87	20.658077401628425	27.158209106250293	28.605045946709957	23.578667545411324
88-89	20.46287251758673	26.959803824202027	28.66184650563533	23.91547715257591
90-91	20.252365715263174	26.771118474586718	28.650332832056918	24.32618297809319
92-93	20.341846468304137	26.880524658658466	28.4675814972375	24.310047375799897
94-95	20.240925942855093	26.599508487037653	28.682837079956318	24.476728490150933
96-97	20.190033465559974	26.583637866850623	28.66595647884434	24.56037218874506
98-99	20.22958373425851	26.549614583133955	28.55718064192912	24.663621040678407
100	20.252401626086776	26.546862426698404	28.428102732791626	24.772633214423195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	812.0
1	659.0
2	1159.5
3	1781.0
4	1158.5
5	653.5
6	1449.0
7	3448.0
8	5500.5
9	6437.5
10	5449.5
11	4040.0
12	3994.5
13	4463.0
14	5100.0
15	5781.0
16	6509.0
17	7395.0
18	8413.5
19	9752.5
20	11559.0
21	14385.5
22	18586.0
23	24657.0
24	32187.0
25	41368.0
26	54442.0
27	71574.5
28	93593.0
29	116261.0
30	135564.5
31	154451.0
32	171835.0
33	188412.5
34	205691.0
35	220903.5
36	232774.5
37	243115.0
38	252875.5
39	258974.5
40	262487.5
41	262532.5
42	259158.5
43	257519.0
44	255028.5
45	251922.0
46	249072.0
47	243920.0
48	238827.0
49	236902.5
50	232373.0
51	222037.5
52	209561.5
53	198901.0
54	191942.0
55	185037.5
56	174198.5
57	157641.0
58	139940.5
59	124116.0
60	105521.5
61	85519.5
62	68999.5
63	55456.5
64	43479.0
65	33520.5
66	25782.0
67	19414.5
68	14528.5
69	10784.0
70	7908.0
71	5816.5
72	4385.0
73	3249.0
74	2385.0
75	1691.5
76	1053.0
77	638.0
78	443.0
79	341.5
80	220.0
81	99.0
82	52.5
83	35.0
84	24.0
85	13.5
86	6.5
87	4.5
88	3.5
89	2.5
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05830338748570523
2	2.6769232087100654E-5
3	0.0
4	0.11561631338418775
5	0.0016463077733566902
6	0.0013652308364421335
7	0.0
8	0.0
9	1.3384616043550327E-5
10-11	0.0
12-13	1.3384616043550327E-5
14-15	6.6923080217751635E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.539230845008288E-4
30-31	2.2753847274035557E-4
32-33	6.6923080217751635E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.2046154439195296E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.1376923637017779E-4
80-81	2.7438462889278173E-4
82-83	3.6807694119763405E-4
84-85	0.0
86-87	0.0
88-89	8.030769626130197E-5
90-91	4.684615615242615E-5
92-93	5.956154139379896E-4
94-95	6.290769540468654E-4
96-97	1.3384616043550327E-5
98-99	4.015384813065098E-5
100	0.0010172308193098249
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7471264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.40870559246214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33827410351847	47.64172873963161
2	14.667244456115855	18.307274822288136
3	4.3639448812447394	8.170444539460055
4	1.717110009368775	4.286504521782632
5	0.8859801046325323	2.7646435755395258
6	0.5050281829417222	1.8910893111063636
7	0.3122748957729086	1.3642070423945778
8	0.23404873534639925	1.1685342894817217
9	0.15948776236337384	0.8958082326242728
>10	0.7595690142338231	8.267515592060427
>50	0.03691449247769559	1.5706138667187033
>100	0.018731033477425104	2.1230414296126976
>500	8.997234401203943E-4	0.3848838558595818
>1k	4.069346250698366E-4	0.41759475529582135
>5k	2.1417610292887358E-5	0.0844225193752044
>10k+	6.425283087866208E-5	0.6616929067685431
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19360	0.25912616660313437	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19273	0.25796170500734544	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10871	0.1455041610094356	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.3384616043550329E-5	0.0	1.3384616043550329E-5	0.0
5	0.0	1.3384616043550329E-5	0.0	1.3384616043550329E-5	0.0
6	0.0	1.3384616043550329E-5	0.0	2.6769232087100657E-5	0.0
7	0.0	2.6769232087100657E-5	0.0	4.015384813065098E-5	0.0
8	0.0	2.6769232087100657E-5	0.0	5.3538464174201315E-5	0.0
9	0.0	2.6769232087100657E-5	0.0	6.692308021775164E-5	0.0
10-11	0.0	2.6769232087100657E-5	0.0	7.361538823952681E-5	0.0
12-13	0.0	2.6769232087100657E-5	0.0	1.0038462032662746E-4	0.0
14-15	0.0	4.015384813065099E-5	0.0	1.2046154439195296E-4	0.0
16-17	0.0	5.3538464174201315E-5	0.0	1.3384616043550328E-4	0.0
18-19	0.0	6.023077219597648E-5	0.0	1.806923165879294E-4	1.3384616043550329E-5
20-21	0.0	6.692308021775164E-5	0.0	3.2123078504520786E-4	2.6769232087100657E-5
22-23	0.0	8.030769626130197E-5	0.0	6.692308021775165E-4	2.6769232087100657E-5
24-25	0.0	8.030769626130197E-5	0.0	0.0013518462203985831	2.6769232087100657E-5
26-27	0.0	8.030769626130197E-5	0.0	0.0030918463060601257	2.6769232087100657E-5
28-29	0.0	9.36923123048523E-5	0.0	0.009623538935312686	2.6769232087100657E-5
30-31	0.0	1.0038462032662746E-4	0.0	0.02954653991613735	2.6769232087100657E-5
32-33	0.0	1.137692363701778E-4	0.0	0.07880192695640256	2.6769232087100657E-5
34-35	0.0	1.271538524137281E-4	0.0	0.1643430080907327	2.6769232087100657E-5
36-37	0.0	1.4053846845727845E-4	0.0	0.3050621688645991	2.6769232087100657E-5
38-39	0.0	1.539230845008288E-4	0.0	0.4934841012176788	2.6769232087100657E-5
40-41	0.0	1.6061539252260393E-4	0.0	0.7042449577474441	2.6769232087100657E-5
42-43	0.0	2.1415385669680526E-4	0.0	0.9279219687592354	2.6769232087100657E-5
44-45	0.0	2.1415385669680526E-4	0.0	1.2032569053911093	2.6769232087100657E-5
46-47	0.0	2.2753847274035557E-4	0.0	1.5362059217824453	2.6769232087100657E-5
48-49	0.0	2.2753847274035557E-4	0.0	1.8935952470693045	2.6769232087100657E-5
50-51	0.0	3.2123078504520786E-4	0.0	2.2569474188035654	2.6769232087100657E-5
52-53	0.0	3.346154010887582E-4	0.0	2.6139485902251614	4.015384813065098E-5
54-55	0.0	3.546923251540837E-4	0.0	3.0172873023895286	4.015384813065098E-5
56-57	0.0	3.613846331758589E-4	0.0	3.4915979411248217	4.015384813065098E-5
58-59	0.0	3.747692492194092E-4	0.0	3.999711427678101	5.3538464174201315E-5
60-61	0.0	3.881538652629595E-4	0.0	4.514925453042483	5.3538464174201315E-5
62-63	0.0	3.881538652629595E-4	0.0	5.020863939488686	5.3538464174201315E-5
64-65	0.0	3.881538652629595E-4	0.0	5.56933204341327	5.3538464174201315E-5
66-67	0.0	4.0153848130650984E-4	0.0	6.186971843050921	5.3538464174201315E-5
68-69	0.0	4.0153848130650984E-4	0.0	6.849744567987425	5.3538464174201315E-5
70-71	0.0	4.149230973500602E-4	0.0	7.529288216826497	6.023077219597648E-5
72-73	0.0	4.149230973500602E-4	0.0	8.230903097521384	6.692308021775164E-5
74-75	0.0	4.2161540537183535E-4	0.0	8.924902131687489	6.692308021775164E-5
76-77	0.0	4.350000214153857E-4	0.0	9.646868321076594	6.692308021775164E-5
78-79	0.0	4.5507694548071114E-4	0.0	10.436540590721998	6.692308021775164E-5
80-81	0.0	4.5507694548071114E-4	0.0	11.25255378474111	6.692308021775164E-5
82-83	0.0	4.5507694548071114E-4	0.0	12.06856697876022	6.692308021775164E-5
84-85	0.0	4.7515386954603665E-4	0.0	12.860079633111614	6.692308021775164E-5
86-87	0.0	4.818461775678118E-4	0.0	13.675724750189527	6.692308021775164E-5
88	0.0	4.952307936113621E-4	0.0	14.321043935805239	6.692308021775164E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13080	0.0	57.91022	1
GTATCAA	24015	0.0	40.597977	1
GTGGTAT	5235	0.0	36.150665	1
TGGTATC	5350	0.0	33.94521	2
ATCAACG	30270	0.0	31.847435	3
TCAACGC	30480	0.0	31.674322	4
TATCAAC	31255	0.0	30.994299	2
CAACGCA	31740	0.0	30.413975	5
AACGCAG	33435	0.0	28.970137	6
ACGCAGA	38060	0.0	25.32624	7
CGCAGAG	37935	0.0	25.298195	8
GCAGAGT	40730	0.0	23.123692	9
GTACATG	17720	0.0	22.661686	1
TACATGG	17790	0.0	21.448153	2
GAGTACT	31035	0.0	20.928118	12-13
CAGAGTA	40025	0.0	20.624846	10-11
AGAGTAC	38120	0.0	20.441158	10-11
GCGGTAT	470	0.0	20.032679	1
ACATGGG	18295	0.0	19.930319	3
AGTACTT	32335	0.0	18.938492	12-13
>>END_MODULE