FastQCFastQC Report
Wed 25 May 2016
SRR2049491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140024
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.039.9717831
GTATCAA51200.034.1901471
TCAACGC55000.030.2392884
TCGCTCT13300.030.0119274
GTCTCGC13500.029.9528471
ATCAACG56000.029.7906863
CAACGCA58300.028.6148935
TCTGTCG13950.027.889558
TATCAAC62050.027.5458132
GTGGTAT21050.026.0181541
AACGCAG64400.025.9839386
TACCTGG21250.025.7677042
TCTCGCT16250.025.5083622
GTACCTG23350.025.4282171
CTCGCTC15100.025.0787373
CGCTCTG14300.024.6919945
CTGTCGC17150.023.2132439
GTACATG68850.023.1950571
CGCAGAG72700.023.0864268
ACGCAGA72150.022.9800937
Produced by FastQC (version 0.11.5)