FastQCFastQC Report
Wed 25 May 2016
SRR2049491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140024
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137510.4379265890961343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127700.40668478967039745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80710.25703625195221436No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79100.25190890260711385No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50780.16171850915789177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.11531758992924894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.057.4042241
ATAACGC4050.042.9854743
TATAACG4300.041.5805442
GTGGTAT24950.038.8724371
GTATCAA120900.038.747341
TAACGCA5800.034.0718964
TGGTATC29650.030.6272242
ATCAACG152800.030.5774273
TCAACGC153550.030.4587174
TATCAAC156200.029.941972
TACCTGG25850.029.8509622
CAACGCA159050.029.5494965
GTACCTG26950.028.8250681
AACGCAG166600.028.2945486
GTCTCGC13000.025.7134361
ACGCAGA186650.025.204387
CGCAGAG187150.024.9863688
TCGCTCT13850.023.7806264
GCAGAGT200000.023.2635029
TCTGTCG12250.022.6352798
Produced by FastQC (version 0.11.5)