##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049491_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3140024 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.826126488205187 31.0 31.0 34.0 30.0 34.0 2 31.975868655780975 33.0 31.0 34.0 30.0 34.0 3 31.932641916112743 33.0 31.0 34.0 30.0 34.0 4 35.616274588984034 37.0 35.0 37.0 33.0 37.0 5 35.73601698585743 37.0 35.0 37.0 35.0 37.0 6 35.788600341908214 37.0 35.0 37.0 35.0 37.0 7 35.803421247735685 37.0 35.0 37.0 35.0 37.0 8 35.79441080705116 37.0 35.0 37.0 35.0 37.0 9 37.531439568614765 39.0 37.0 39.0 35.0 39.0 10-11 37.48531285111197 39.0 37.0 39.0 35.0 39.0 12-13 37.46504453469146 39.0 37.0 39.0 34.0 39.0 14-15 38.72759316489301 40.0 38.0 41.0 34.5 41.0 16-17 38.72828901944698 40.0 38.0 41.0 34.5 41.0 18-19 38.815696950087 40.0 38.0 41.0 35.0 41.0 20-21 38.829453851308145 40.0 38.0 41.0 35.0 41.0 22-23 38.7658704200987 40.0 38.0 41.0 35.0 41.0 24-25 38.70478840289118 40.0 38.0 41.0 34.0 41.0 26-27 38.58953625832159 40.0 38.0 41.0 34.0 41.0 28-29 38.477234568907754 40.0 38.0 41.0 34.0 41.0 30-31 38.31969803415515 40.0 38.0 41.0 34.0 41.0 32-33 38.1016619618194 40.0 37.5 41.0 34.0 41.0 34-35 37.9445814745365 40.0 37.0 41.0 33.0 41.0 36-37 37.755927343230496 40.0 37.0 41.0 33.0 41.0 38-39 37.620515161667555 40.0 37.0 41.0 33.0 41.0 40-41 37.47388316140258 40.0 37.0 41.0 33.0 41.0 42-43 37.313055091298665 40.0 37.0 41.0 32.5 41.0 44-45 37.094918223554984 40.0 37.0 41.0 31.5 41.0 46-47 36.79110000433118 40.0 36.5 41.0 31.0 41.0 48-49 36.513541457007975 40.0 36.0 41.0 30.0 41.0 50-51 36.28488715372876 40.0 36.0 41.0 30.0 41.0 52-53 36.161815005235624 39.5 35.5 41.0 30.0 41.0 54-55 36.012948627144254 39.0 35.0 41.0 29.0 41.0 56-57 35.83370079336973 39.0 35.0 41.0 29.0 41.0 58-59 35.657318542788204 39.0 35.0 41.0 28.0 41.0 60-61 35.442295663982186 39.0 35.0 41.0 28.0 41.0 62-63 35.19442972410402 38.0 35.0 40.0 28.0 41.0 64-65 34.88282032239244 37.5 34.5 40.0 28.0 41.0 66-67 34.6166344588449 37.0 34.0 40.0 27.5 41.0 68-69 34.28306360078776 36.5 34.0 39.5 26.5 41.0 70-71 33.963453941753315 36.0 34.0 39.0 26.0 41.0 72-73 33.628435324061215 36.0 34.0 39.0 26.0 41.0 74-75 33.307017239358686 35.0 34.0 38.5 26.0 40.0 76-77 33.033264395431374 35.0 33.5 37.0 26.0 39.5 78-79 32.72055500212737 35.0 33.0 37.0 26.0 39.0 80-81 32.43025276239926 35.0 33.0 36.5 25.0 39.0 82-83 32.15819608385159 35.0 33.0 36.0 25.0 38.0 84-85 31.897596961042336 35.0 33.0 36.0 24.5 37.0 86-87 31.6300762350861 35.0 33.0 35.0 24.0 37.0 88-89 31.4391566752356 35.0 33.0 35.0 23.5 36.5 90-91 31.301833361783224 35.0 33.0 35.0 23.0 36.0 92-93 31.14678024753951 35.0 33.0 35.0 22.5 36.0 94-95 30.95886448638609 35.0 33.0 35.0 20.0 36.0 96-97 30.784569958700953 35.0 32.5 35.0 19.0 35.5 98-99 30.606881030208687 35.0 32.0 35.0 16.5 35.0 100 30.49268317694387 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3683719798061986 1101 2 0.37275819132184296 1101 3 0.4852534916573852 1101 4 0.32959612036695063 1101 5 0.30248725186965686 1101 6 0.32495530196309375 1101 7 0.3042298164681867 1101 8 0.34607960643806024 1101 9 0.33816405093134705 1101 10-11 0.2980756583454891 1101 12-13 0.3269379949704998 1101 14-15 0.5026765296944475 1101 16-17 0.47646615098580725 1101 18-19 0.4930838462075968 1101 20-21 0.4194875988769198 1101 22-23 0.47461941297279253 1101 24-25 0.5140210242465386 1101 26-27 0.4747501649338517 1101 28-29 0.46020465261604215 1101 30-31 0.4651898497391187 1101 32-33 0.4611826663208376 1101 34-35 0.47545958734039573 1101 36-37 0.5183316225483381 1101 38-39 0.5359172940568371 1101 40-41 0.542011360781629 1101 42-43 0.4565490950321731 1101 44-45 0.465213120508686 1101 46-47 0.5630235767277512 1101 48-49 0.4820058219889063 1101 50-51 0.5467804016815876 1101 52-53 0.5592605464228271 1101 54-55 0.49868260548227283 1101 56-57 0.5019432522879015 1101 58-59 0.44913250623729795 1101 60-61 0.4745920471452507 1101 62-63 0.46384876831646693 1101 64-65 0.4624507734820611 1101 66-67 0.5171581785391126 1101 68-69 0.489591894198405 1101 70-71 0.46385296848897184 1101 72-73 0.49800837440055545 1101 74-75 0.5270911235913758 1101 76-77 0.45775365212606545 1101 78-79 0.5068277467930358 1101 80-81 0.5152002343479793 1101 82-83 0.5513628573083267 1101 84-85 0.5188379623547519 1101 86-87 0.5329535924798314 1101 88-89 0.4642676195607258 1101 90-91 0.48384173837893485 1101 92-93 0.5443154043227345 1101 94-95 0.4631619263175537 1101 96-97 0.4594442936026404 1101 98-99 0.5012304385512607 1101 100 0.49040521712127116 1102 1 0.29387738296514243 1102 2 0.33895374502890974 1102 3 0.4083854583350579 1102 4 0.37205808568273824 1102 5 0.32186778085609546 1102 6 0.3402531131050779 1102 7 0.3072360364434985 1102 8 0.3285290189073464 1102 9 0.39473506317299467 1102 10-11 0.3400963224880158 1102 12-13 0.3389393723841394 1102 14-15 0.37499706249583653 1102 16-17 0.4560429089561424 1102 18-19 0.47915914691877504 1102 20-21 0.4825360121127673 1102 22-23 0.41758081664207225 1102 24-25 0.4594170338179211 1102 26-27 0.36112995925770974 1102 28-29 0.391803967518527 1102 30-31 0.4795421054428246 1102 32-33 0.5332781732957272 1102 34-35 0.5212817788508346 1102 36-37 0.5322641909736276 1102 38-39 0.5227081091168131 1102 40-41 0.4709666982909937 1102 42-43 0.5416108404737869 1102 44-45 0.5359224004029599 1102 46-47 0.4819183806425116 1102 48-49 0.4126891169453444 1102 50-51 0.3859613252999736 1102 52-53 0.39119006078379215 1102 54-55 0.40842565614292425 1102 56-57 0.376084860651261 1102 58-59 0.3221417458625737 1102 60-61 0.4431323412159287 1102 62-63 0.44946891817419754 1102 64-65 0.4498684964141759 1102 66-67 0.4592467900520205 1102 68-69 0.43273727827835984 1102 70-71 0.4525204676752992 1102 72-73 0.5196017045624473 1102 74-75 0.5058624492135095 1102 76-77 0.4705039614880562 1102 78-79 0.4488689336434746 1102 80-81 0.43741565647049896 1102 82-83 0.4626580689073023 1102 84-85 0.40637121470243187 1102 86-87 0.48665888555480663 1102 88-89 0.518758609189625 1102 90-91 0.5135880709941105 1102 92-93 0.5095429493221282 1102 94-95 0.5034835389743151 1102 96-97 0.5721653717456832 1102 98-99 0.5853858693151324 1102 100 0.6520712748916431 1103 1 0.2538283507311192 1103 2 0.24261129778300727 1103 3 0.31342038474013023 1103 4 0.28214820828154785 1103 5 0.20107229391137338 1103 6 0.19398846458847885 1103 7 0.2324903301557697 1103 8 0.23653688139839346 1103 9 0.24830669713260534 1103 10-11 0.25246305014629655 1103 12-13 0.22031159860227234 1103 14-15 0.33836513998839024 1103 16-17 0.3622960982585539 1103 18-19 0.33153271689631936 1103 20-21 0.31950515019517667 1103 22-23 0.3042037989779942 1103 24-25 0.35476154327688647 1103 26-27 0.3106985327019771 1103 28-29 0.34525751629075785 1103 30-31 0.3576599269812064 1103 32-33 0.33068983670627006 1103 34-35 0.31767949721376 1103 36-37 0.3314733553480451 1103 38-39 0.3987265596868781 1103 40-41 0.36308758236966554 1103 42-43 0.3646298685607121 1103 44-45 0.3771729491654625 1103 46-47 0.3626097667315662 1103 48-49 0.3155420542624867 1103 50-51 0.35387431060042474 1103 52-53 0.35640899677580506 1103 54-55 0.2702371819376026 1103 56-57 0.2637980275495835 1103 58-59 0.2405724005058758 1103 60-61 0.17442551323333078 1103 62-63 0.19356131763118611 1103 64-65 0.22009277423461526 1103 66-67 0.2971273598902471 1103 68-69 0.29888099047246897 1103 70-71 0.3176044202395474 1103 72-73 0.29448281951827227 1103 74-75 0.23762799235474574 1103 76-77 0.28096301044009664 1103 78-79 0.20423187220799832 1103 80-81 0.22271667801924622 1103 82-83 0.24025515329766023 1103 84-85 0.24128229087634168 1103 86-87 0.26245122977104884 1103 88-89 0.28997060890450754 1103 90-91 0.26722726754099924 1103 92-93 0.22927641881336314 1103 94-95 0.29832766207206873 1103 96-97 0.30190029110391947 1103 98-99 0.3971314727352002 1103 100 0.3172031277406724 1104 1 0.2569136254783402 1104 2 0.2738527344292905 1104 3 0.355679229087972 1104 4 0.2668364964003729 1104 5 0.16214749699348374 1104 6 0.2067228972822619 1104 7 0.12329770056174993 1104 8 0.18295220347338415 1104 9 0.22819544320217489 1104 10-11 0.2611785848246839 1104 12-13 0.2363376973821616 1104 14-15 0.30772618607607427 1104 16-17 0.35527044709532873 1104 18-19 0.3379189467913051 1104 20-21 0.27318030938647553 1104 22-23 0.30821834274573234 1104 24-25 0.3293336078172828 1104 26-27 0.3851960089800315 1104 28-29 0.38791774970164994 1104 30-31 0.36591529025318437 1104 32-33 0.43877532626304117 1104 34-35 0.36668938483934 1104 36-37 0.3197420687433379 1104 38-39 0.40047682210286695 1104 40-41 0.3871815214891541 1104 42-43 0.3555556164462885 1104 44-45 0.40249761638482795 1104 46-47 0.3979137890113762 1104 48-49 0.43235911664318394 1104 50-51 0.5636938938002345 1104 52-53 0.4949782113159742 1104 54-55 0.4327363256022281 1104 56-57 0.4670225221085147 1104 58-59 0.47891876465102 1104 60-61 0.4340409983313549 1104 62-63 0.5102561998226705 1104 64-65 0.46469880480955794 1104 66-67 0.4765676474795981 1104 68-69 0.45196857297094795 1104 70-71 0.46562380360840905 1104 72-73 0.5313488120312897 1104 74-75 0.5389181994776067 1104 76-77 0.5548515742118312 1104 78-79 0.4379943747044379 1104 80-81 0.4251700353183594 1104 82-83 0.45572916250312545 1104 84-85 0.442943610526882 1104 86-87 0.4782670474174786 1104 88-89 0.5099997417241617 1104 90-91 0.5184919587725858 1104 92-93 0.46223657860844725 1104 94-95 0.49540889375520436 1104 96-97 0.4213420214006618 1104 98-99 0.4881298784999899 1104 100 0.5410191497464609 1105 1 0.28502794788519736 1105 2 0.2699814562834746 1105 3 0.3502958605053159 1105 4 0.264801291616763 1105 5 0.22961825188576768 1105 6 0.2449854988284983 1105 7 0.2499222721821397 1105 8 0.20110438729599878 1105 9 0.23976190737371184 1105 10-11 0.20394910975543468 1105 12-13 0.24509467794295148 1105 14-15 0.3069341607977023 1105 16-17 0.26771706565403974 1105 18-19 0.25494375540220915 1105 20-21 0.26121279861507674 1105 22-23 0.24764937992025438 1105 24-25 0.2517977617256477 1105 26-27 0.24048546055901454 1105 28-29 0.2537724176330798 1105 30-31 0.29222487976269207 1105 32-33 0.23689183623898202 1105 34-35 0.22265187928052654 1105 36-37 0.30304532590147915 1105 38-39 0.29089635980897555 1105 40-41 0.3045798175050649 1105 42-43 0.2751818692239567 1105 44-45 0.2944806976373613 1105 46-47 0.33714179750461426 1105 48-49 0.32348248092493037 1105 50-51 0.3076142959788868 1105 52-53 0.33662312408115014 1105 54-55 0.2491752693427287 1105 56-57 0.23819616104270125 1105 58-59 0.18677944503372856 1105 60-61 0.29082532744938305 1105 62-63 0.32976978003349444 1105 64-65 0.3473479718514483 1105 66-67 0.3921390797295672 1105 68-69 0.41891143473351633 1105 70-71 0.4170463382609597 1105 72-73 0.4120005405936382 1105 74-75 0.37014694288271244 1105 76-77 0.3737749051348942 1105 78-79 0.2856797387073513 1105 80-81 0.3383728382833411 1105 82-83 0.34727414998897643 1105 84-85 0.29900819925809685 1105 86-87 0.4236854588618719 1105 88-89 0.3208351448968507 1105 90-91 0.2977988311032256 1105 92-93 0.29191488532326204 1105 94-95 0.34308430582663973 1105 96-97 0.40351089755849756 1105 98-99 0.43590364998740583 1105 100 0.4325295026549405 1106 1 0.24499761543113152 1106 2 0.24641908545171987 1106 3 0.3280023042909157 1106 4 0.2736412569067923 1106 5 0.20528799942767506 1106 6 0.21429065922253443 1106 7 0.23535365563845545 1106 8 0.2218738772601725 1106 9 0.24920112250484294 1106 10-11 0.23192446275346157 1106 12-13 0.21506355461201565 1106 14-15 0.2762029231330061 1106 16-17 0.34067000715108264 1106 18-19 0.3344489899215688 1106 20-21 0.2960771100637203 1106 22-23 0.22504299535072647 1106 24-25 0.24769634516992056 1106 26-27 0.3001387552036334 1106 28-29 0.34445103650951125 1106 30-31 0.34057672116551885 1106 32-33 0.4589018952180197 1106 34-35 0.47612869141315173 1106 36-37 0.38683950562370484 1106 38-39 0.4637010949682363 1106 40-41 0.48247888522296023 1106 42-43 0.4545586360971541 1106 44-45 0.4669407256791587 1106 46-47 0.4869187458585884 1106 48-49 0.4307970729133288 1106 50-51 0.4789020318269053 1106 52-53 0.4336006230803733 1106 54-55 0.37664711955375196 1106 56-57 0.499328240359894 1106 58-59 0.4856680304208467 1106 60-61 0.4452320955831226 1106 62-63 0.5142964623803508 1106 64-65 0.5101954786065761 1106 66-67 0.572240573379176 1106 68-69 0.5019875558568643 1106 70-71 0.5177593680349304 1106 72-73 0.4776942071763415 1106 74-75 0.5303697574872075 1106 76-77 0.4926073670636981 1106 78-79 0.49254406256189753 1106 80-81 0.42685412651320576 1106 82-83 0.37568138378745886 1106 84-85 0.39311629949875027 1106 86-87 0.5413707062612296 1106 88-89 0.4458296010050695 1106 90-91 0.4919296385929428 1106 92-93 0.4746661709412443 1106 94-95 0.49849141135686637 1106 96-97 0.5036155281314976 1106 98-99 0.5437317137989872 1106 100 0.5522831478330197 1107 1 0.15204133227028294 1107 2 0.18734318451901544 1107 3 0.24239940731901655 1107 4 0.20656976733333465 1107 5 0.0965036113356561 1107 6 0.11532423531512137 1107 7 0.12639442708834991 1107 8 0.20690484419515087 1107 9 0.1600375323577765 1107 10-11 0.150379881798969 1107 12-13 0.1378807387391916 1107 14-15 0.18162348168102227 1107 16-17 0.1886848954885494 1107 18-19 0.18902880122836763 1107 20-21 0.21476873184345635 1107 22-23 0.21018239699288443 1107 24-25 0.23619049810152148 1107 26-27 0.2540624104451439 1107 28-29 0.24689258263713754 1107 30-31 0.222858658128807 1107 32-33 0.22773086762684613 1107 34-35 0.19575016182140814 1107 36-37 0.2297361076666533 1107 38-39 0.19669954157801328 1107 40-41 0.2511187273285742 1107 42-43 0.21025355967170611 1107 44-45 0.26745532821971807 1107 46-47 0.23075982260682792 1107 48-49 0.2203412772883695 1107 50-51 0.2498341332074716 1107 52-53 0.21140361823190545 1107 54-55 0.15388272379564683 1107 56-57 0.13441434928549967 1107 58-59 0.12560932437472871 1107 60-61 0.20309403214292843 1107 62-63 0.18501724216879012 1107 64-65 0.17441942233485008 1107 66-67 0.08963215548358505 1107 68-69 0.18784128163978409 1107 70-71 0.15902289295306105 1107 72-73 0.162177788984863 1107 74-75 0.19158947142132376 1107 76-77 0.16544640624813667 1107 78-79 0.19947615049320433 1107 80-81 0.25553569496661055 1107 82-83 0.24451069242794432 1107 84-85 0.18882278944205666 1107 86-87 0.22672680920191013 1107 88-89 0.2256205657881658 1107 90-91 0.2388010030882235 1107 92-93 0.2017570274577487 1107 94-95 0.2402806344600137 1107 96-97 0.24352521631026036 1107 98-99 0.3339214829423369 1107 100 0.27461968881629417 1108 1 0.06791626465296474 1108 2 0.06786335991291992 1108 3 0.1878652929625524 1108 4 0.1816294227502624 1108 5 0.09672977308822084 1108 6 0.14153562576262146 1108 7 0.08633403433366738 1108 8 0.10653560360595549 1108 9 0.11328228931460416 1108 10-11 0.081551756155271 1108 12-13 0.07260316344204654 1108 14-15 0.12154300256785433 1108 16-17 0.20010307933065263 1108 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0.08288600356849685 1108 84-85 0.08077151092192736 1108 86-87 0.11175773450951354 1108 88-89 0.08757162119006168 1108 90-91 0.08159311032891381 1108 92-93 0.14825674135052225 1108 94-95 0.13254621359972774 1108 96-97 0.09449441610626508 1108 98-99 0.18425246940608986 1108 100 0.13843929009564704 1109 1 0.08638989436752453 1109 2 0.15046260106002052 1109 3 0.21136740989349434 1109 4 0.039259648157489835 1109 5 0.09532142734048676 1109 6 0.08491740577567697 1109 7 0.0864702850028678 1109 8 0.10865425044357124 1109 9 0.08799179553400904 1109 10-11 0.16085987975109362 1109 12-13 0.17726240930215198 1109 14-15 0.18525289625392816 1109 16-17 0.18199554924029115 1109 18-19 0.1965892435433787 1109 20-21 0.26167294568445953 1109 22-23 0.21325055674862625 1109 24-25 0.20011427183495556 1109 26-27 0.22535305882452406 1109 28-29 0.11534934714980949 1109 30-31 0.16400128749749854 1109 32-33 0.24366716333508975 1109 34-35 0.2381634228939049 1109 36-37 0.2188943235129699 1109 38-39 0.1809706932300159 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-0.638450741167091 2215 24-25 -0.5634880165672982 2215 26-27 -0.5496600324162273 2215 28-29 -0.46245117021366156 2215 30-31 -0.5086904880602248 2215 32-33 -0.5696235372286509 2215 34-35 -0.5703491230514572 2215 36-37 -0.5892196660567421 2215 38-39 -0.6964096368175134 2215 40-41 -0.5976957936058085 2215 42-43 -0.5699975097849332 2215 44-45 -0.709555854356303 2215 46-47 -0.6061013599234357 2215 48-49 -0.49445116681476975 2215 50-51 -0.5108889601300604 2215 52-53 -0.6501935448133551 2215 54-55 -0.6662379270222232 2215 56-57 -0.5179954635073329 2215 58-59 -0.5435762593406679 2215 60-61 -0.7824646420572989 2215 62-63 -0.7478806275828234 2215 64-65 -0.5051888061275065 2215 66-67 -0.3948400908162242 2215 68-69 -0.43515416411182173 2215 70-71 -0.5109366185674205 2215 72-73 -0.41875035384163795 2215 74-75 -0.46535475818089367 2215 76-77 -0.47363181076377003 2215 78-79 -0.5361626509587154 2215 80-81 -0.5375144920757968 2215 82-83 -0.6045861617021657 2215 84-85 -0.5345342701006075 2215 86-87 -0.5273435483982709 2215 88-89 -0.48736663154744164 2215 90-91 -0.4981832954791088 2215 92-93 -0.5392154801613955 2215 94-95 -0.6024509657143824 2215 96-97 -0.6645997556585499 2215 98-99 -0.60408214410295 2215 100 -0.7102692497024208 2216 1 -0.6264852699890362 2216 2 -0.9795827807940469 2216 3 -0.6533480011012003 2216 4 -1.1050002976929463 2216 5 -0.6521661350299439 2216 6 -0.8536677728473947 2216 7 -0.5801142033834097 2216 8 -0.5352397666130528 2216 9 -0.583175688966044 2216 10-11 -0.8811370949945072 2216 12-13 -0.6620042414453238 2216 14-15 -0.9735604281111847 2216 16-17 -0.8816321615796596 2216 18-19 -1.0090315229840314 2216 20-21 -0.7693875581884058 2216 22-23 -0.8440506416871543 2216 24-25 -0.8510881133706434 2216 26-27 -0.9785570779347168 2216 28-29 -1.1543612880867826 2216 30-31 -1.2415494376156957 2216 32-33 -1.0760290565247743 2216 34-35 -0.9901664987496304 2216 36-37 -1.0045725730705968 2216 38-39 -1.086573913930465 2216 40-41 -1.0171144689000187 2216 42-43 -1.5466593516190912 2216 44-45 -1.2621333200325182 2216 46-47 -1.0394556250279763 2216 48-49 -0.9010841056577448 2216 50-51 -1.1002877324673932 2216 52-53 -0.9768647825940917 2216 54-55 -1.1479927134484456 2216 56-57 -1.1869309933732026 2216 58-59 -1.0284569754288952 2216 60-61 -1.106633512031621 2216 62-63 -1.0869021679994688 2216 64-65 -1.1081347490442042 2216 66-67 -0.9745653597508053 2216 68-69 -1.3359082236765474 2216 70-71 -1.1301225281048062 2216 72-73 -0.9972486780452883 2216 74-75 -0.9403747838148178 2216 76-77 -1.1021483901485034 2216 78-79 -1.1796768478994295 2216 80-81 -0.9426634220113073 2216 82-83 -1.0708175618404034 2216 84-85 -0.9857278689795663 2216 86-87 -1.1662714708931432 2216 88-89 -0.9692976965264073 2216 90-91 -0.9243512994650018 2216 92-93 -1.0429965122266651 2216 94-95 -1.086218561854853 2216 96-97 -1.118752973156866 2216 98-99 -1.1721235046694716 2216 100 -1.1575223712059497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 165.0 3 25.0 4 42.0 5 96.0 6 368.0 7 881.0 8 1831.0 9 3033.0 10 4360.0 11 6372.0 12 9507.0 13 13721.0 14 13664.0 15 15266.0 16 18390.0 17 25579.0 18 26029.0 19 14528.0 20 10830.0 21 10107.0 22 10333.0 23 11245.0 24 12725.0 25 15608.0 26 19223.0 27 24266.0 28 31359.0 29 39970.0 30 51808.0 31 66081.0 32 84553.0 33 109611.0 34 149297.0 35 218086.0 36 343884.0 37 643298.0 38 828159.0 39 304684.0 40 1040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45241462471073 19.46743970687865 13.053211416130726 23.026934252279894 2 17.550084888021377 20.89487356605167 37.99034337053374 23.564698175393207 3 18.113906135749282 25.215221284932852 30.68779092134328 25.983081657974587 4 13.102575302981508 18.06519084407572 37.4101577321264 31.422076120816367 5 11.985923454804578 35.56193918741391 34.116322582186164 18.335814775595345 6 28.901714958698506 37.47459759824889 18.345430625049563 15.278256818003038 7 25.25665408926811 32.234435150814136 23.10577880933394 19.40313195058382 8 24.51831578357363 33.04500220380481 22.425242609610628 20.011439403010932 9 23.956034716943154 16.559815007724467 23.491770601680308 35.99237967365207 10-11 22.96781808037136 25.94449915032497 29.12479331368168 21.962889455621994 12-13 24.85187210412153 23.83234135546141 29.1632417979104 22.152544742506663 14-15 22.362042455726453 25.96878240421092 27.71762254046466 23.951552599597964 16-17 20.38014677594821 28.821865692746297 27.795201565338356 23.00278596596714 18-19 19.8827142722476 26.228079785377435 31.917319740231285 21.97188620214368 20-21 21.735311577236352 26.3117415663065 31.160924247712757 20.792022608744393 22-23 21.084010822847212 26.79350539995873 31.207739176515847 20.914744600678212 24-25 20.820254877032788 26.172809507188482 31.751238207096506 21.255697408682224 26-27 20.69428450228406 25.643068333235668 32.03357681342563 21.62907035105464 28-29 20.947509888472048 25.935296590467576 31.74745702256673 21.36973649849364 30-31 21.522419963732645 27.021551496267215 30.817521832705204 20.638506707294933 32-33 20.396710406785175 26.329771098846873 31.885414233364813 21.388104261003143 34-35 20.396563847919634 25.457337268759726 31.93238331936317 22.21371556395747 36-37 21.954195254558563 26.65009885274762 30.503779588945818 20.891926303747997 38-39 21.553593220943533 26.561994430615815 30.830990463767154 21.053421884673494 40-41 20.838694226540944 26.263589068109034 31.182691597261673 21.715025108088344 42-43 20.767898589310146 26.413763079517867 30.828156090526697 21.990182240645296 44-45 21.517192225282354 26.830575180317094 30.337061118004193 21.31517147639636 46-47 21.254789772307472 27.799819364437976 29.4403800735281 21.505010789726448 48-49 21.17810246036336 26.866609936739337 30.080279641174716 21.875007961722588 50-51 21.346461046157607 26.464431482052365 29.880997724858155 22.30810974693187 52-53 21.30636581121673 26.825208979294423 29.518532342427957 22.349892867060888 54-55 21.667684705594606 27.8885766478218 29.028870479970852 21.414868166612738 56-57 20.798774149496946 27.373930899891214 29.193550749930573 22.633744200681267 58-59 20.53663713657647 26.51950188175703 30.022730765035543 22.921130216630957 60-61 21.718560112916336 27.38458368471069 28.90874400959993 21.98811219277305 62-63 21.381651859985784 27.23073135746733 29.120159591136886 22.267457191410003 64-65 21.17883493884123 26.606866699108032 29.826842087831174 22.38745627421956 66-67 21.12332580897471 26.882931468039732 29.098567399484843 22.895175323500712 68-69 22.08596176334958 27.306415492365666 28.77770997928678 21.829912764997975 70-71 22.17288785053872 26.730798872874857 29.069937045067174 22.026376231519247 72-73 21.477702081257977 26.989570780350725 29.656126832151603 21.876600306239695 74-75 21.40832363064741 26.831036960227056 29.40582619750677 22.354813211618765 76-77 21.61470740351029 27.17867761520294 28.729000160508328 22.47761482077844 78-79 21.91990815345125 27.504585958051226 28.738272367691923 21.837233520805597 80-81 21.367419566107376 26.91861651045464 29.203493038969704 22.51047088446828 82-83 21.110864650741153 27.017718235924782 29.2736161803126 22.597800933021468 84-85 21.476937755889765 27.073375872286327 28.935192852029157 22.514493519794755 86-87 21.78361085514169 27.223458677936645 28.773431154257285 22.21949931266438 88-89 21.097339620126803 27.088588406162188 28.967333503926646 22.846738469784363 90-91 20.814472000514648 26.81610192540052 28.969048624500083 23.40037744958475 92-93 21.310397045862224 27.297366801459553 28.485168403528345 22.90706774914988 94-95 21.169599471082464 26.92705536755119 28.897805030449064 23.005540130917282 96-97 20.893757499942677 26.900702032850702 29.10793038524546 23.097610081961157 98-99 20.884780002442657 26.84962389612689 29.01916179926679 23.246434302163664 100 21.331710399262164 27.094718712659137 28.54408546263812 23.029485425440573 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1389.0 1 1114.5 2 3584.5 3 5610.5 4 3091.0 5 1270.5 6 1963.0 7 4237.5 8 6556.5 9 7136.5 10 5396.5 11 3363.5 12 2973.0 13 3207.0 14 3589.0 15 4021.0 16 4405.0 17 4817.0 18 5385.5 19 6048.0 20 6826.5 21 7831.5 22 9246.5 23 11726.5 24 14734.5 25 18185.0 26 23022.5 27 28940.5 28 37019.5 29 45587.0 30 52434.0 31 59253.5 32 66294.0 33 72920.0 34 79049.0 35 84148.0 36 87785.0 37 90850.0 38 93567.5 39 95212.5 40 96837.5 41 96791.0 42 95052.0 43 94345.0 44 93598.0 45 93060.5 46 94105.5 47 96138.5 48 98199.0 49 101453.5 50 103310.5 51 100541.0 52 95395.5 53 91325.0 54 88762.5 55 85521.5 56 80886.5 57 75764.0 58 70707.5 59 65276.0 60 57335.5 61 47493.0 62 38306.0 63 29969.5 64 22917.5 65 17218.0 66 12826.5 67 9465.5 68 6831.0 69 4869.5 70 3511.5 71 2578.0 72 1875.5 73 1404.5 74 1051.0 75 684.5 76 443.5 77 338.0 78 270.5 79 223.5 80 132.0 81 43.5 82 21.0 83 13.5 84 8.5 85 8.0 86 4.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06171927348325999 2 1.592344517111971E-4 3 0.0 4 0.11764241292423243 5 0.0015604976267697317 6 0.0020700478722455624 7 0.0 8 0.0 9 3.184689034223942E-5 10-11 0.0 12-13 3.184689034223942E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.2738756136895769E-4 30-31 3.184689034223942E-4 32-33 1.592344517111971E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.0700478722455627E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.2738756136895769E-4 80-81 2.3885167756679566E-4 82-83 3.3439234859351395E-4 84-85 0.0 86-87 1.592344517111971E-5 88-89 7.961722585559855E-5 90-91 6.369378068447884E-5 92-93 6.847081423581475E-4 94-95 8.280191488982251E-4 96-97 0.0 98-99 1.592344517111971E-5 100 0.0010191004909516615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3140024.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.44558950452667 #Duplication Level Percentage of deduplicated Percentage of total 1 82.77196909737114 57.48148188417402 2 11.34126636018652 15.752018562220204 3 2.722003548927175 5.670934232659841 4 1.0946943549116894 3.0408677921647924 5 0.5724539172756031 1.9877199874689901 6 0.34627583520501476 1.4428397704190747 7 0.23770070422542933 1.1555085871403252 8 0.16704490933505567 0.928042576200252 9 0.11726900580582686 0.7329433714915865 >10 0.5906628502601959 7.0368679232386295 >50 0.02615692104961852 1.2307225102978536 >100 0.01144693593486094 1.3905896874724595 >500 5.96620593988138E-4 0.30060994810611685 >1k 2.294694588880624E-4 0.3324174889026837 >5k 1.376816753328374E-4 0.6711773632599825 >10k+ 9.178778355522494E-5 0.8452583147831326 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13751 0.4379265890961343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12770 0.40668478967039745 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8071 0.25703625195221436 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7910 0.25190890260711385 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5078 0.16171850915789177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3621 0.11531758992924894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.184689034223942E-5 0.0 8 0.0 0.0 0.0 3.184689034223942E-5 0.0 9 0.0 0.0 0.0 6.369378068447884E-5 0.0 10-11 0.0 0.0 0.0 6.369378068447884E-5 0.0 12-13 0.0 0.0 0.0 1.1146411619783797E-4 0.0 14-15 0.0 0.0 0.0 1.7515789688231682E-4 0.0 16-17 0.0 0.0 0.0 2.3885167756679566E-4 0.0 18-19 0.0 0.0 0.0 2.866220130801548E-4 0.0 20-21 0.0 0.0 0.0 4.2993301962023216E-4 0.0 22-23 0.0 0.0 0.0 8.28019148898225E-4 0.0 24-25 0.0 0.0 0.0 0.001703808633309809 0.0 26-27 0.0 0.0 0.0 0.002579598117721393 0.0 28-29 0.0 0.0 0.0 0.005413971358180702 0.0 30-31 0.0 0.0 0.0 0.013104995375831523 0.0 32-33 0.0 0.0 0.0 0.031432880767790314 0.0 34-35 0.0 0.0 0.0 0.06111418256675745 0.0 36-37 0.0 0.0 0.0 0.10270622135372215 0.0 38-39 0.0 0.0 0.0 0.1585815904591812 0.0 40-41 0.0 0.0 0.0 0.23135173489119826 0.0 42-43 0.0 0.0 0.0 0.30695625256367465 0.0 44-45 0.0 0.0 0.0 0.38932823443387693 0.0 46-47 0.0 0.0 0.0 0.4845026662216595 0.0 48-49 0.0 0.0 0.0 0.5892152416669427 0.0 50-51 0.0 0.0 0.0 0.700456429632385 0.0 52-53 0.0 0.0 0.0 0.8080033783181275 0.0 54-55 0.0 0.0 0.0 0.9339737530668555 0.0 56-57 0.0 0.0 0.0 1.0738930657854846 0.0 58-59 0.0 0.0 0.0 1.2248791728980415 0.0 60-61 0.0 0.0 0.0 1.3759926675719676 0.0 62-63 0.0 0.0 0.0 1.5280456455109896 0.0 64-65 0.0 0.0 0.0 1.7010857241855475 0.0 66-67 0.0 0.0 0.0 1.9045873534724576 0.0 68-69 0.0 0.0 0.0 2.114776829731238 0.0 70-71 0.0 0.0 0.0 2.3382146123723895 0.0 72-73 0.0 0.0 0.0 2.5972412949709938 0.0 74-75 0.0 1.592344517111971E-5 0.0 2.8540546186908125 0.0 76-77 0.0 3.184689034223942E-5 0.0 3.113638621870406 0.0 78-79 0.0 3.184689034223942E-5 0.0 3.4006268741894967 0.0 80-81 0.0 3.184689034223942E-5 0.0 3.7115002942652664 0.0 82-83 0.0 3.184689034223942E-5 0.0 4.0346347671228 0.0 84-85 0.0 4.777033551335913E-5 0.0 4.359855211297749 0.0 86-87 0.0 6.369378068447884E-5 0.0 4.709916229939644 0.0 88 0.0 6.369378068447884E-5 0.0 4.990853573093709 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6955 0.0 57.404224 1 ATAACGC 405 0.0 42.985474 3 TATAACG 430 0.0 41.580544 2 GTGGTAT 2495 0.0 38.872437 1 GTATCAA 12090 0.0 38.74734 1 TAACGCA 580 0.0 34.071896 4 TGGTATC 2965 0.0 30.627224 2 ATCAACG 15280 0.0 30.577427 3 TCAACGC 15355 0.0 30.458717 4 TATCAAC 15620 0.0 29.94197 2 TACCTGG 2585 0.0 29.850962 2 CAACGCA 15905 0.0 29.549496 5 GTACCTG 2695 0.0 28.825068 1 AACGCAG 16660 0.0 28.294548 6 GTCTCGC 1300 0.0 25.713436 1 ACGCAGA 18665 0.0 25.20438 7 CGCAGAG 18715 0.0 24.986368 8 TCGCTCT 1385 0.0 23.780626 4 GCAGAGT 20000 0.0 23.263502 9 TCTGTCG 1225 0.0 22.635279 8 >>END_MODULE