FastQCFastQC Report
Wed 25 May 2016
SRR2049490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153543
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66550.042.8504561
GTATCAA110500.035.9540441
TCAACGC113950.033.6000374
ATCAACG115500.033.3267023
CAACGCA124850.030.7895875
TATCAAC128700.030.4264532
AACGCAG135750.028.4296826
ACGCAGA155650.024.4978317
CGCAGAG156550.024.1278638
GTGGTAT41900.021.9941331
GTACATG168600.020.5275651
TGGTATC41200.020.2515752
GCAGAGT179650.019.8923159
TACATGG166500.019.7988552
ACATGGG169300.018.7754823
TCTGTCG18000.018.0944548
AGAGTAC154000.017.69790310-11
CAGAGTA171550.016.96849310-11
CTGTCGC19450.016.7455089
CATGGGG115650.015.6678754
Produced by FastQC (version 0.11.5)