##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049490_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6153543 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07033964010652 31.0 31.0 34.0 28.0 34.0 2 31.164019004986233 31.0 31.0 34.0 28.0 34.0 3 31.32129522780616 31.0 31.0 34.0 28.0 34.0 4 34.89897348568134 37.0 35.0 37.0 32.0 37.0 5 34.9195573996964 37.0 35.0 37.0 32.0 37.0 6 34.93117623456926 37.0 35.0 37.0 32.0 37.0 7 30.89315927425875 37.0 33.0 37.0 2.0 37.0 8 32.700275272310606 37.0 33.0 37.0 17.0 37.0 9 35.531339262600426 39.0 35.0 39.0 30.0 39.0 10-11 36.101231518167666 39.0 36.0 39.0 32.0 39.0 12-13 36.280286738875475 39.0 37.0 39.0 32.0 39.0 14-15 37.493287363068724 40.0 38.0 41.0 32.0 41.0 16-17 37.51316680487973 40.0 38.0 41.0 32.0 41.0 18-19 37.51625413196918 40.0 38.0 41.0 32.0 41.0 20-21 37.59475573665448 40.0 38.0 41.0 32.0 41.0 22-23 37.5534371824492 40.0 38.0 41.0 32.0 41.0 24-25 37.53110110711829 40.0 38.0 41.0 32.0 41.0 26-27 37.56055292048825 40.0 38.0 41.0 32.0 41.0 28-29 37.54175342562813 40.0 38.0 41.0 32.0 41.0 30-31 37.46281166800979 40.0 38.0 41.0 32.0 41.0 32-33 37.40669367224703 40.0 38.0 41.0 32.0 41.0 34-35 37.341529018323264 40.0 37.0 41.0 31.0 41.0 36-37 37.16255781425433 40.0 37.0 41.0 30.5 41.0 38-39 37.048824067695634 40.0 37.0 41.0 30.0 41.0 40-41 36.97040704842722 40.0 37.0 41.0 30.0 41.0 42-43 36.85297656975827 40.0 37.0 41.0 30.0 41.0 44-45 36.73665545198921 40.0 36.5 41.0 30.0 41.0 46-47 36.58057536934413 40.0 36.0 41.0 30.0 41.0 48-49 36.4965540177423 40.0 36.0 41.0 30.0 41.0 50-51 36.364205547925806 40.0 36.0 41.0 30.0 41.0 52-53 36.2490279989918 39.5 35.5 41.0 30.0 41.0 54-55 36.13237642119345 39.0 35.0 41.0 29.0 41.0 56-57 35.93700799685645 39.0 35.0 41.0 29.0 41.0 58-59 35.76386311105651 39.0 35.0 41.0 29.0 41.0 60-61 35.542747730860086 39.0 35.0 40.5 28.0 41.0 62-63 35.30812769164041 38.5 35.0 40.0 28.0 41.0 64-65 34.94843978501491 38.0 34.0 40.0 27.5 41.0 66-67 31.411086913669084 36.5 31.0 40.0 7.0 41.0 68-69 31.776475438621297 36.0 31.0 39.5 11.0 41.0 70-71 31.92850663430807 36.0 30.0 39.0 15.5 41.0 72-73 33.175686104736734 35.0 32.5 39.0 25.5 41.0 74-75 33.250936736770996 35.0 33.0 39.0 26.0 40.0 76-77 32.92319172873253 35.0 33.0 37.5 25.5 39.5 78-79 29.502529518360397 35.0 29.0 37.0 6.5 39.0 80-81 31.26452427487709 35.0 30.0 36.0 21.5 39.0 82-83 31.63232588120372 35.0 32.0 36.0 22.5 38.0 84-85 29.74078104272612 35.0 30.5 36.0 11.5 37.0 86-87 29.323855947703624 35.0 28.5 35.0 11.5 37.0 88-89 30.513366852234558 35.0 30.5 35.0 18.0 36.5 90-91 30.602258487508742 35.0 32.0 35.0 14.5 36.0 92-93 30.391552638861874 35.0 32.0 35.0 4.5 36.0 94-95 30.1298864735324 35.0 32.0 35.0 2.0 36.0 96-97 29.877028729627796 35.0 31.5 35.0 2.0 35.0 98-99 29.623325212808297 35.0 31.0 35.0 2.0 35.0 100 29.382523856581486 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3646171014136321 1101 2 0.3452372035748503 1101 3 0.32854516656969324 1101 4 0.349176192233692 1101 5 0.36652280689861527 1101 6 0.4019859917495836 1101 7 -12.179344592205478 1101 8 -6.581128401252283 1101 9 -2.142395929528327 1101 10-11 -0.0805081657961253 1101 12-13 0.4378009698120664 1101 14-15 0.630086712590419 1101 16-17 0.6562401998032996 1101 18-19 0.6570381059308019 1101 20-21 0.6510031710050939 1101 22-23 0.570162347352067 1101 24-25 0.7007849587450323 1101 26-27 0.6484191744661132 1101 28-29 0.5599384351520555 1101 30-31 0.6487321374667161 1101 32-33 0.6002651449376302 1101 34-35 0.6281996352139956 1101 36-37 0.6711915694372124 1101 38-39 0.6634678363406437 1101 40-41 0.6089290230550404 1101 42-43 0.6372407041518287 1101 44-45 0.588850875517295 1101 46-47 0.5963617803780892 1101 48-49 0.5498370936655235 1101 50-51 0.6106828000126896 1101 52-53 0.6233387966628783 1101 54-55 0.5091926328243304 1101 56-57 0.48983139659785735 1101 58-59 0.4386452781855752 1101 60-61 0.43186963742667217 1101 62-63 0.45523893868460164 1101 64-65 0.35123587294852854 1101 66-67 -10.343093334272854 1101 68-69 -8.196502091903337 1101 70-71 -6.7274468195916235 1101 72-73 -1.4766008878465868 1101 74-75 -0.2193176483170447 1101 76-77 0.09938529129898654 1101 78-79 3.2492984323032736 1101 80-81 1.1435526636637583 1101 82-83 0.4326432366832087 1101 84-85 -5.3083154400860195 1101 86-87 -5.818011592657488 1101 88-89 -1.202826074109229 1101 90-91 0.12901412805205226 1101 92-93 0.4788825764756943 1101 94-95 0.5676348837790144 1101 96-97 0.6408840533183557 1101 98-99 0.6279248919259572 1101 100 0.6416319612809112 1102 1 0.23472187179066495 1102 2 0.2968238949756987 1102 3 0.2684427835691423 1102 4 0.2624014409279738 1102 5 0.2593278440968234 1102 6 0.3453005344174329 1102 7 -12.350181209797064 1102 8 -6.726838809161311 1102 9 -2.277189116808877 1102 10-11 -0.21555096824376818 1102 12-13 0.3059217181631979 1102 14-15 0.4847626677764296 1102 16-17 0.514997100899997 1102 18-19 0.4922226553951887 1102 20-21 0.4663423487948464 1102 22-23 0.42882312331035166 1102 24-25 0.4941401172784623 1102 26-27 0.4629502100834415 1102 28-29 0.4586547356158519 1102 30-31 0.4528997573299094 1102 32-33 0.48013960272344036 1102 34-35 0.48886411797585794 1102 36-37 0.6066302761770856 1102 38-39 0.6328725196421345 1102 40-41 0.581169463717103 1102 42-43 0.5141334893449994 1102 44-45 0.5329765376542284 1102 46-47 0.5354550426034166 1102 48-49 0.49060930886839316 1102 50-51 0.5629069098563733 1102 52-53 0.569129155373119 1102 54-55 0.5074806457017687 1102 56-57 0.572320370906688 1102 58-59 0.5608728195367618 1102 60-61 0.5241017665843017 1102 62-63 0.4784291716006024 1102 64-65 0.3722154146276324 1102 66-67 -10.413186880933875 1102 68-69 -6.751285818304456 1102 70-71 -6.266908982720892 1102 72-73 -0.9774575461066632 1102 74-75 -0.02846357130393784 1102 76-77 0.15414803122063603 1102 78-79 3.318186367687904 1102 80-81 1.1922049384014919 1102 82-83 0.5407653227406506 1102 84-85 -5.338667532419329 1102 86-87 -5.858155577493495 1102 88-89 -1.26031850346698 1102 90-91 0.09580759062867727 1102 92-93 0.40768570598837783 1102 94-95 0.4482256791409682 1102 96-97 0.556457267397338 1102 98-99 0.52214985729238 1102 100 0.6239070341346711 1103 1 0.14571292891490373 1103 2 0.16616280745135725 1103 3 0.18741974839089437 1103 4 0.15673818537749895 1103 5 0.1601099379777864 1103 6 0.1972439344817687 1103 7 -12.348654521594227 1103 8 -6.748790796465563 1103 9 -2.3767381275922546 1103 10-11 -0.31274241967030036 1103 12-13 0.24111398031215714 1103 14-15 0.3794685269632012 1103 16-17 0.2875235687199904 1103 18-19 0.389947732973404 1103 20-21 0.385862341178246 1103 22-23 0.35550093022793305 1103 24-25 0.4030172362090809 1103 26-27 0.37590936420583887 1103 28-29 0.3863590465535012 1103 30-31 0.3645975114139546 1103 32-33 0.3539817640558809 1103 34-35 0.31349064066614574 1103 36-37 0.4302102323179895 1103 38-39 0.4119775611051395 1103 40-41 0.41667002979071555 1103 42-43 0.4131463611880051 1103 44-45 0.4107623186382341 1103 46-47 0.43535788586029867 1103 48-49 0.3345264905351968 1103 50-51 0.35763442941435386 1103 52-53 0.3265649082059383 1103 54-55 0.317650047440587 1103 56-57 0.37575699068476354 1103 58-59 0.3217035819579621 1103 60-61 0.26951616588458904 1103 62-63 0.20939396842538827 1103 64-65 0.09798648936659049 1103 66-67 -10.586617926166458 1103 68-69 -8.464822608846081 1103 70-71 -6.985790680363593 1103 72-73 -1.7100849736660777 1103 74-75 -0.5201737156242032 1103 76-77 -0.2047221990887209 1103 78-79 2.9703898446979444 1103 80-81 0.8594429738807428 1103 82-83 0.13598698650644891 1103 84-85 -5.636548078024653 1103 86-87 -6.128532762326802 1103 88-89 -1.550502503248456 1103 90-91 -0.2850526039075767 1103 92-93 0.1263054769016101 1103 94-95 0.1983247585983996 1103 96-97 0.324322432614192 1103 98-99 0.36893633204475407 1103 100 0.4084744671731606 1104 1 0.28434990078442723 1104 2 0.31420988963802543 1104 3 0.33537086657624826 1104 4 0.3043459991407502 1104 5 0.34550117592743845 1104 6 0.2778547288310307 1104 7 -12.322708524210245 1104 8 -6.678400697633997 1104 9 -2.313577219063305 1104 10-11 -0.2022841485122342 1104 12-13 0.3352016258175965 1104 14-15 0.5042150381111199 1104 16-17 0.5334916519835318 1104 18-19 0.5503558854871571 1104 20-21 0.5361332038133639 1104 22-23 0.5652683894129353 1104 24-25 0.6564834972663647 1104 26-27 0.6290640005694996 1104 28-29 0.6159100784056832 1104 30-31 0.5707928213089275 1104 32-33 0.5676150685519659 1104 34-35 0.5819424529098924 1104 36-37 0.6495860823513695 1104 38-39 0.6378410649098925 1104 40-41 0.6389464915802563 1104 42-43 0.5377242624503182 1104 44-45 0.5232605997562558 1104 46-47 0.5514075238554597 1104 48-49 0.4511270509353764 1104 50-51 0.48533767907715486 1104 52-53 0.5587735994855549 1104 54-55 0.5034805729477796 1104 56-57 0.49142651020654426 1104 58-59 0.459490558713064 1104 60-61 0.4309855606008739 1104 62-63 0.3764180434882931 1104 64-65 0.23323830972320536 1104 66-67 -10.516316341784599 1104 68-69 -8.358305928611191 1104 70-71 -6.745261618055494 1104 72-73 -1.6096820090506796 1104 74-75 -0.3537387291622238 1104 76-77 -0.07643063256792004 1104 78-79 3.0840098892472803 1104 80-81 0.9376065777697171 1104 82-83 0.2442099967426934 1104 84-85 -5.567190571236505 1104 86-87 -6.01817443506749 1104 88-89 -1.450845641841493 1104 90-91 -0.13621231668144063 1104 92-93 0.22836339640002024 1104 94-95 0.22744616562480502 1104 96-97 0.33091607028892156 1104 98-99 0.3959625463025347 1104 100 0.48222052522506687 1105 1 0.15976253821855835 1105 2 0.15676755215277183 1105 3 0.14012195369538816 1105 4 0.20081905715341009 1105 5 0.18706275319196664 1105 6 0.21648051179317207 1105 7 -12.257976689199918 1105 8 -6.674491491271176 1105 9 -2.4526746189930364 1105 10-11 -0.35158757841394817 1105 12-13 0.16756363247164785 1105 14-15 0.3293596116772761 1105 16-17 0.41112580514171526 1105 18-19 0.41246087124970643 1105 20-21 0.39663041553490075 1105 22-23 0.39847690649129675 1105 24-25 0.42237433310662453 1105 26-27 0.3857984861865802 1105 28-29 0.3937083289272536 1105 30-31 0.4116631952350289 1105 32-33 0.45460840621606735 1105 34-35 0.448505778483252 1105 36-37 0.5362354356842189 1105 38-39 0.45597011982896163 1105 40-41 0.45047659441249266 1105 42-43 0.3534478819929703 1105 44-45 0.34663057894223925 1105 46-47 0.2832507031118183 1105 48-49 0.3050616900137655 1105 50-51 0.3746511931267307 1105 52-53 0.3678153106010953 1105 54-55 0.3255882244244219 1105 56-57 0.3228065886477438 1105 58-59 0.29801029182001315 1105 60-61 0.29353517723998834 1105 62-63 0.24389726358904085 1105 64-65 0.14735828537602913 1105 66-67 -10.42958580478697 1105 68-69 -8.400258804195445 1105 70-71 -6.830861309914539 1105 72-73 -1.7169864981716927 1105 74-75 -0.4734222133821291 1105 76-77 -0.15839425629189918 1105 78-79 2.9611096873450258 1105 80-81 0.8505469322950638 1105 82-83 0.13232146014175683 1105 84-85 -5.538778317090149 1105 86-87 -6.001866478325159 1105 88-89 -1.5321337117231657 1105 90-91 -0.22459608984135215 1105 92-93 0.121412297146275 1105 94-95 0.1753647826406386 1105 96-97 0.26370055851323926 1105 98-99 0.33938578007201414 1105 100 0.4198567288388375 1106 1 0.14005348093536796 1106 2 0.14951227554989188 1106 3 0.17714483655220903 1106 4 0.1085061531270668 1106 5 0.19596988002908233 1106 6 0.20187645411808575 1106 7 -12.293921850434387 1106 8 -6.737148864086944 1106 9 -2.562645928154275 1106 10-11 -0.3973678134029157 1106 12-13 0.19156490094890444 1106 14-15 0.29875589907044997 1106 16-17 0.36557541009580774 1106 18-19 0.3427792137082193 1106 20-21 0.3713485570402568 1106 22-23 0.32971157180487154 1106 24-25 0.4413405441565956 1106 26-27 0.37231187440689695 1106 28-29 0.3191328480610949 1106 30-31 0.3011583776836986 1106 32-33 0.3404606261490031 1106 34-35 0.3858506280858123 1106 36-37 0.39794991203778807 1106 38-39 0.3703152436616506 1106 40-41 0.41601926661290634 1106 42-43 0.33803826167231676 1106 44-45 0.2741909118982022 1106 46-47 0.3598275705808476 1106 48-49 0.277681461373227 1106 50-51 0.3546958895662655 1106 52-53 0.3410806537583042 1106 54-55 0.30596999539999814 1106 56-57 0.3943844577176563 1106 58-59 0.3815262939766271 1106 60-61 0.34963576440524236 1106 62-63 0.2676322840241241 1106 64-65 0.16615663984630658 1106 66-67 -10.4693633809724 1106 68-69 -8.406507115855664 1106 70-71 -6.85499990289987 1106 72-73 -1.6453156598745693 1106 74-75 -0.38007363914609726 1106 76-77 -0.12633359139273637 1106 78-79 3.00961037180333 1106 80-81 0.8722080291858028 1106 82-83 0.15659727291388137 1106 84-85 -5.586976485035532 1106 86-87 -6.00255461350628 1106 88-89 -1.4335500158680254 1106 90-91 -0.19404954341892022 1106 92-93 0.13301905718894957 1106 94-95 0.2172123262330814 1106 96-97 0.2345693900340997 1106 98-99 0.2775585561317442 1106 100 0.40355506936489505 1107 1 0.14804266165997504 1107 2 0.20096409892996547 1107 3 0.16957181830654378 1107 4 0.1531247499429753 1107 5 0.18377019796393057 1107 6 0.1838343633405728 1107 7 -12.31368790733049 1107 8 -6.765414695581477 1107 9 -2.424380333924553 1107 10-11 -0.420982780067213 1107 12-13 0.2533564875386958 1107 14-15 0.3853691811545872 1107 16-17 0.41510728662319707 1107 18-19 0.42048486298337906 1107 20-21 0.4062158073783593 1107 22-23 0.4636340336645475 1107 24-25 0.5230870504322525 1107 26-27 0.409069176231462 1107 28-29 0.391528510860347 1107 30-31 0.45170622213289136 1107 32-33 0.45065874226640545 1107 34-35 0.41956904220780444 1107 36-37 0.48083196770010517 1107 38-39 0.4748107000405142 1107 40-41 0.5518142223630207 1107 42-43 0.5553189696705161 1107 44-45 0.47963210581473703 1107 46-47 0.5133253284283796 1107 48-49 0.5045028246968357 1107 50-51 0.5272699755035788 1107 52-53 0.48616689951719394 1107 54-55 0.4489054356448534 1107 56-57 0.46809433030644954 1107 58-59 0.4488333383688712 1107 60-61 0.41638120298924264 1107 62-63 0.42371670284396856 1107 64-65 0.2736394974636909 1107 66-67 -10.390931701374804 1107 68-69 -8.29112761079006 1107 70-71 -6.764768569708892 1107 72-73 -1.6159128554045026 1107 74-75 -0.27310422258026534 1107 76-77 -0.07327714609414215 1107 78-79 3.1175354910288995 1107 80-81 1.0114383421380957 1107 82-83 0.30559388002707877 1107 84-85 -5.505513035679705 1107 86-87 -5.946300313081576 1107 88-89 -1.3555757828265236 1107 90-91 -0.03951944637761429 1107 92-93 0.3554775951631832 1107 94-95 0.4622133939247348 1107 96-97 0.465136452933006 1107 98-99 0.49848507521969765 1107 100 0.5701609962398813 1108 1 0.06764739925158025 1108 2 0.08109029577388682 1108 3 0.11889186044934519 1108 4 0.08769504390058103 1108 5 0.06006394716796137 1108 6 0.0753141081682358 1108 7 -12.302744441201028 1108 8 -6.739603620361208 1108 9 -2.5819043286914365 1108 10-11 -0.5247455300006365 1108 12-13 0.07631366165560394 1108 14-15 0.2300352226258795 1108 16-17 0.20359350338075188 1108 18-19 0.273030913253713 1108 20-21 0.25313324685597394 1108 22-23 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10-11 -0.7492414854542488 2216 12-13 -1.1177176056700873 2216 14-15 -1.3583924024545198 2216 16-17 -1.417978143641399 2216 18-19 -1.940423559856434 2216 20-21 -1.5172694607066575 2216 22-23 -1.3468233479109983 2216 24-25 -1.348402255593271 2216 26-27 -1.762444451559169 2216 28-29 -1.93683379086918 2216 30-31 -1.8410439887367644 2216 32-33 -2.7909258339179175 2216 34-35 -1.8840663764466683 2216 36-37 -1.5510983312822901 2216 38-39 -1.7705213310983794 2216 40-41 -1.763510480699921 2216 42-43 -1.4182017286532371 2216 44-45 -1.575425590500899 2216 46-47 -1.6685914883423152 2216 48-49 -1.7977643951180298 2216 50-51 -2.349600667148515 2216 52-53 -2.0493565045576716 2216 54-55 -1.682925771408712 2216 56-57 -1.7030383771667914 2216 58-59 -2.046602842467564 2216 60-61 -2.1770921757192028 2216 62-63 -1.9876172138393784 2216 64-65 -1.6944272661176853 2216 66-67 1.764849116235748 2216 68-69 0.8846015120801063 2216 70-71 0.5210507965806173 2216 72-73 -1.2667185455494554 2216 74-75 -2.10812150329005 2216 76-77 -1.7810732156182922 2216 78-79 0.8740698179190041 2216 80-81 -0.6315348139354491 2216 82-83 -1.5067257792303188 2216 84-85 0.3420599179796504 2216 86-87 0.35802808711721923 2216 88-89 -0.9158355597572054 2216 90-91 -1.2314293487957748 2216 92-93 -1.3097230012191154 2216 94-95 -1.261866224043068 2216 96-97 -1.2701986656168565 2216 98-99 -1.2588002475802398 2216 100 -2.3524368764977623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73472.0 3 14515.0 4 8395.0 5 7712.0 6 7147.0 7 8313.0 8 9867.0 9 11549.0 10 13105.0 11 14722.0 12 16591.0 13 18573.0 14 21717.0 15 23199.0 16 24278.0 17 25158.0 18 23568.0 19 22119.0 20 21168.0 21 21278.0 22 23407.0 23 26415.0 24 31636.0 25 40162.0 26 50120.0 27 62164.0 28 78731.0 29 100892.0 30 125191.0 31 153945.0 32 204805.0 33 288303.0 34 435838.0 35 604810.0 36 658367.0 37 1117513.0 38 1308022.0 39 455838.0 40 938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.459681769914894 21.06416078042488 15.013390837731524 22.462766611928693 2 15.460503916805088 20.86826751641421 40.027256666564284 23.643971900216417 3 17.927964031744267 25.69796077827214 31.120289737781846 25.25378545220175 4 12.717232130683234 17.943072940527184 39.38485545268351 29.95483947610607 5 12.878504098790247 37.7559011675007 35.51518907999007 13.850405653718974 6 29.49181717762956 38.57394312693463 18.37394367948214 13.560296015953671 7 26.337148613909285 32.92169318804266 22.98941136905682 17.75174682899123 8 24.558404808416874 35.87710364581835 21.23345851325001 18.331033032514764 9 24.24983460747735 17.71265756979353 22.45421865094629 35.58328917178282 10-11 23.204436858570745 27.522274566050807 29.34329052384943 19.92999805152901 12-13 23.90761712398857 25.033919483458554 29.17342090564736 21.885042486905512 14-15 21.501247330196605 27.545180069433172 26.42720624524766 24.52636635512257 16-17 20.8326169418964 30.719558185014574 27.0820420682386 21.365782804850422 18-19 20.75782195720417 29.577310177242605 29.05196242229883 20.612905443254398 20-21 22.03848254574641 28.85601026920589 28.44706699863802 20.65844018640968 22-23 21.72084439809716 28.79284340744836 28.481323686208093 21.004988508246388 24-25 21.46341546650442 28.814278213380483 28.479747358554253 21.242558961560846 26-27 21.216409952394834 28.77332765204515 28.51782406662578 21.492438328934234 28-29 21.268194599436455 28.774699388628633 28.669694840842098 21.287411171092817 30-31 21.659603256205408 29.20321674846507 28.17669430440317 20.96048569092635 32-33 21.167262597113776 28.94837404633185 28.606960949729366 21.27740240682501 34-35 21.357922210967637 28.61012057935423 28.59379664489113 21.438160564787008 36-37 21.75531588148744 28.865732938850503 28.08302112831927 21.295930051342786 38-39 21.50559328360695 28.95874099156892 28.309626928625324 21.226038796198807 40-41 21.307651543184146 28.72174615501996 28.522340706809068 21.44826159498682 42-43 21.236786978685362 28.68694393301808 28.44766708731874 21.62860200097781 44-45 21.458380088316744 28.8040545163242 28.35590546113915 21.3816599342199 46-47 21.348480311758422 28.944845459461373 28.256040380386228 21.45063384839398 48-49 21.22305659818695 28.629360749271026 28.495185098694225 21.652397553847802 50-51 21.21553265937269 28.53546776323065 28.439262734714067 21.809736842682597 52-53 21.17680984853237 28.586407207834984 28.419454147102 21.81732879653064 54-55 21.252128153410844 28.911692836395712 28.29839300802931 21.53778600216413 56-57 21.10198679328056 28.636408289744704 28.28579245313835 21.975812463836395 58-59 21.004802774864363 28.270141379105247 28.56355851913196 22.161497326898434 60-61 21.232214371804083 28.536667631706624 28.287929462756594 21.943188533732698 62-63 21.080001605619277 28.579161824484032 28.30221687593617 22.038619693960516 64-65 21.052061512179975 28.361201437137893 28.473479358551636 22.113257692130496 66-67 20.958990552605506 28.41674297591848 28.254304553494908 22.369961917981104 68-69 21.558693266155615 28.580234657892994 28.005236011589357 21.85583606436203 70-71 21.264673881689124 28.354371957457424 28.156829637117255 22.2241245237362 72-73 21.03216514018204 28.40720745375409 28.477509330924715 22.08311807513915 74-75 21.124561143630427 28.506318873543957 28.272056293224917 22.0970636896007 76-77 21.226892376019883 28.585415568315746 27.985553448599777 22.20213860706459 78-79 21.39738137563596 28.58554116226733 28.11664971805866 21.90042774403805 80-81 21.226476842972502 28.43602904723764 28.18581040149964 22.151683708290225 82-83 21.209790826897553 28.4186644118072 28.24971246803914 22.121832293256112 84-85 21.385946813081816 28.312592557958418 28.053130500262206 22.248330128697564 86-87 21.54648823909217 28.361967520239922 27.970088939138275 22.121455301529632 88-89 21.426022907392195 28.272799464558663 28.032673167487037 22.26850446056211 90-91 21.464444533465706 28.146372297370704 27.979893509805613 22.409289659357974 92-93 21.681039018446583 28.30481005540516 27.752318570134843 22.26183235601341 94-95 21.70302415552442 28.01514608936076 27.88447576220227 22.39735399291255 96-97 21.80281087697564 28.01758360267864 27.82761380331824 22.351991717027474 98-99 21.90864847018695 27.995803475158766 27.74959770843936 22.345950346214924 100 22.053849016679315 28.05485988431904 27.53941489940892 22.351876199592727 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1913.0 1 1520.5 2 1297.5 3 1501.5 4 1396.5 5 1244.0 6 1191.0 7 1158.0 8 1185.0 9 1206.5 10 1131.0 11 1026.5 12 1036.5 13 1087.0 14 1181.5 15 1380.0 16 1652.0 17 2121.5 18 2773.5 19 3726.0 20 5231.0 21 7503.5 22 11028.5 23 16429.0 24 23006.0 25 31276.5 26 43435.5 27 59309.5 28 79419.5 29 100934.5 30 119883.0 31 137844.5 32 155140.5 33 172737.0 34 188515.0 35 200831.0 36 210469.5 37 218904.0 38 226041.0 39 229404.5 40 231616.0 41 229709.5 42 223132.5 43 218197.5 44 213451.0 45 207803.5 46 202931.0 47 197293.5 48 191596.0 49 188215.5 50 182793.0 51 173064.0 52 162770.5 53 154550.5 54 148077.0 55 139369.0 56 127483.5 57 113708.5 58 100924.5 59 90120.5 60 77544.5 61 63795.5 62 52088.5 63 42361.5 64 34366.5 65 27343.0 66 21304.5 67 16629.5 68 12895.5 69 10031.0 70 7723.0 71 5965.5 72 4642.5 73 3494.5 74 2614.5 75 1905.5 76 1311.0 77 940.0 78 686.5 79 501.0 80 370.5 81 264.5 82 190.0 83 135.0 84 103.5 85 64.5 86 47.5 87 45.0 88 36.5 89 30.0 90 25.5 91 18.5 92 12.0 93 13.0 94 13.0 95 11.0 96 8.5 97 10.0 98 12.5 99 19.0 100 29.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01953346226718494 2 0.003022648903241596 3 0.0019013436649422942 4 0.002600128088809975 5 0.0023076136788188527 6 0.0036726809254440896 7 11.618899876055144 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.7626360943605984E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.462572049955611E-4 28-29 0.0 30-31 0.0 32-33 8.125400277531172E-6 34-35 3.250160111012469E-5 36-37 1.6250800555062344E-5 38-39 3.250160111012469E-5 40-41 0.0 42-43 2.4376200832593514E-5 44-45 1.6250800555062345E-4 46-47 9.831734335812717E-4 48-49 6.825336233126185E-4 50-51 3.0063981026865334E-4 52-53 2.7626360943605984E-4 54-55 4.468970152642144E-4 56-57 0.001373192646902768 58-59 2.7626360943605984E-4 60-61 0.001373192646902768 62-63 0.002502623285479601 64-65 0.0010156750346913966 66-67 5.809669323835066 68-69 5.462511596977547 70-71 0.002892642498801097 72-73 0.0013000640444049876 74-75 0.003112028306294439 76-77 0.006159053410368628 78-79 5.411614089639091 80-81 0.00527338478011773 82-83 0.005102751374289576 84-85 5.809864333441726 86-87 0.0038026873298845883 88-89 0.0018607166635546384 90-91 0.003355790314620374 92-93 0.003827063530717182 94-95 0.004412092350699426 96-97 0.0031932823090697507 98-99 0.005793410397879725 100 0.006711580629240748 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6153543.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.14674112494355 #Duplication Level Percentage of deduplicated Percentage of total 1 92.60826392753697 82.55724930378587 2 5.388952299071992 9.608150710800805 3 1.1014254883658057 2.9456547863928297 4 0.40038645538218975 1.4277259075155933 5 0.1841659509074238 0.8208897174786586 6 0.10545027404362069 0.5640328969032605 7 0.0654686067147929 0.4085419054230069 8 0.03866105837354159 0.2757205889953954 9 0.02403284927755682 0.19282051737370298 >10 0.08164677022544334 1.0953116029231769 >50 0.0013090592179570433 0.07345066531222184 >100 2.37260882742967E-4 0.030451397095445608 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 8.125400277531172E-6 0.0 0.0 0.0 12-13 0.0 4.062700138765586E-5 0.0 8.125400277531172E-6 0.0 14-15 0.0 9.750480333037406E-5 0.0 4.875240166518703E-5 0.0 16-17 0.0 1.137556038854364E-4 0.0 8.937940305284289E-5 0.0 18-19 0.0 1.3000640444049875E-4 0.0 1.0563020360790522E-4 0.0 20-21 0.0 1.3000640444049875E-4 0.0 1.7875880610568578E-4 0.0 22-23 0.0 1.3000640444049875E-4 0.0 6.256558213699002E-4 0.0 24-25 0.0 1.6250800555062345E-4 0.0 0.001893218264664763 0.0 26-27 0.0 1.7875880610568578E-4 0.0 0.003762060328496933 0.0 28-29 0.0 1.7875880610568578E-4 0.0 0.009856110536645311 0.0 30-31 0.0 1.7875880610568578E-4 0.0 0.026188165094482966 0.0 32-33 0.0 1.7875880610568578E-4 0.0 0.06617325986021386 0.0 34-35 0.0 1.9500960666074812E-4 0.0 0.13172898929933535 1.6250800555062344E-5 36-37 0.0 2.1126040721581045E-4 0.0 0.23764358191695417 1.6250800555062344E-5 38-39 0.0 2.4376200832593517E-4 0.0 0.3879228600498932 1.6250800555062344E-5 40-41 0.0 3.087652105461845E-4 0.0 0.5533072572987627 1.6250800555062344E-5 42-43 8.125400277531172E-6 3.4126681165630923E-4 0.0 0.7310666391703121 1.6250800555062344E-5 44-45 1.6250800555062344E-5 3.4126681165630923E-4 0.0 0.956513995270692 1.6250800555062344E-5 46-47 1.6250800555062344E-5 3.737684127664339E-4 0.0 1.2299337146096159 1.6250800555062344E-5 48-49 1.6250800555062344E-5 4.062700138765586E-4 0.0 1.5223912467987954 1.6250800555062344E-5 50-51 1.6250800555062344E-5 4.062700138765586E-4 0.0 1.819423379344225 1.6250800555062344E-5 52-53 1.6250800555062344E-5 4.062700138765586E-4 0.0 2.115699849663844 1.6250800555062344E-5 54-55 1.6250800555062344E-5 4.062700138765586E-4 0.0 2.4435435000616716 1.6250800555062344E-5 56-57 1.6250800555062344E-5 4.062700138765586E-4 0.0 2.7956983480898727 1.6250800555062344E-5 58-59 1.6250800555062344E-5 4.2252081443162095E-4 0.0 3.1755851872652876 1.6250800555062344E-5 60-61 1.6250800555062344E-5 4.306462147091521E-4 0.0 3.5617854624563443 1.6250800555062344E-5 62-63 1.6250800555062344E-5 4.387716149866833E-4 0.0 3.9423710860556267 1.6250800555062344E-5 64-65 1.6250800555062344E-5 4.387716149866833E-4 0.0 4.352533166665123 1.6250800555062344E-5 66-67 1.6250800555062344E-5 4.550224155417456E-4 0.0 4.811658909347022 1.6250800555062344E-5 68-69 1.6250800555062344E-5 4.550224155417456E-4 0.0 5.255744536115211 1.6250800555062344E-5 70-71 1.6250800555062344E-5 4.631478158192768E-4 0.0 5.777452111734654 1.6250800555062344E-5 72-73 1.6250800555062344E-5 5.037748172069327E-4 0.0 6.327882977335171 1.6250800555062344E-5 74-75 1.6250800555062344E-5 5.037748172069327E-4 0.0 6.818811536703327 1.6250800555062344E-5 76-77 1.6250800555062344E-5 5.20025617761995E-4 0.0 7.314119686821072 1.6250800555062344E-5 78-79 1.6250800555062344E-5 5.362764183170573E-4 0.0 7.881662645406069 1.6250800555062344E-5 80-81 1.6250800555062344E-5 5.525272188721197E-4 0.0 8.54506907646538 1.6250800555062344E-5 82-83 1.6250800555062344E-5 5.850288199822443E-4 0.0 9.203096492540965 1.6250800555062344E-5 84-85 1.6250800555062344E-5 6.012796205373067E-4 0.0 9.847270101143359 1.6250800555062344E-5 86-87 2.4376200832593514E-5 6.012796205373067E-4 0.0 10.577296689078146 1.6250800555062344E-5 88 3.250160111012469E-5 6.17530421092369E-4 0.0 11.171044063558181 1.6250800555062344E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6655 0.0 42.850456 1 GTATCAA 11050 0.0 35.954044 1 TCAACGC 11395 0.0 33.600037 4 ATCAACG 11550 0.0 33.326702 3 CAACGCA 12485 0.0 30.789587 5 TATCAAC 12870 0.0 30.426453 2 AACGCAG 13575 0.0 28.429682 6 ACGCAGA 15565 0.0 24.497831 7 CGCAGAG 15655 0.0 24.127863 8 GTGGTAT 4190 0.0 21.994133 1 GTACATG 16860 0.0 20.527565 1 TGGTATC 4120 0.0 20.251575 2 GCAGAGT 17965 0.0 19.892315 9 TACATGG 16650 0.0 19.798855 2 ACATGGG 16930 0.0 18.775482 3 TCTGTCG 1800 0.0 18.094454 8 AGAGTAC 15400 0.0 17.697903 10-11 CAGAGTA 17155 0.0 16.968493 10-11 CTGTCGC 1945 0.0 16.745508 9 CATGGGG 11565 0.0 15.667875 4 >>END_MODULE