FastQCFastQC Report
Wed 25 May 2016
SRR2049490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153543
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189740.3083426897317529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188690.30663635567347136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84700.13764428070137805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127050.055.7404141
GTATCAA229800.040.1608661
ATCAACG277300.032.989433
TCAACGC280200.032.664794
GTGGTAT53300.032.5983921
TATCAAC287500.032.03182
CAACGCA290800.031.5004065
AACGCAG303250.030.34596
TGGTATC55600.029.6224982
CGCAGAG341400.026.5545658
ACGCAGA344500.026.4793197
GCAGAGT367250.024.4422979
GAGTACT254900.022.81633612-13
CAGAGTA359000.021.67885610-11
GTACATG174300.020.7742791
GTACTTT278200.020.75336614-15
AGAGTAC339950.020.37759410-11
TACATGG177500.019.194142
AGTACTT267650.018.91997312-13
ACATGGG177450.018.3509483
Produced by FastQC (version 0.11.5)