##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049490_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6153543 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82174285610745 31.0 31.0 34.0 30.0 34.0 2 31.989329724355546 33.0 31.0 34.0 30.0 34.0 3 31.941960265817595 33.0 31.0 34.0 30.0 34.0 4 35.61702745881519 37.0 35.0 37.0 33.0 37.0 5 35.741367371610146 37.0 35.0 37.0 35.0 37.0 6 35.79777828805292 37.0 35.0 37.0 35.0 37.0 7 35.808574344893664 37.0 35.0 37.0 35.0 37.0 8 35.797255012275045 37.0 35.0 37.0 35.0 37.0 9 37.53708132046855 39.0 37.0 39.0 35.0 39.0 10-11 37.49193212755644 39.0 37.0 39.0 34.5 39.0 12-13 37.4746311190155 39.0 37.0 39.0 34.5 39.0 14-15 38.74473413121514 40.0 38.0 41.0 34.5 41.0 16-17 38.74721765980996 40.0 38.0 41.0 35.0 41.0 18-19 38.82565312373701 40.0 38.0 41.0 35.0 41.0 20-21 38.85952231096785 40.0 38.0 41.0 35.0 41.0 22-23 38.83372627769076 40.0 38.0 41.0 35.0 41.0 24-25 38.79431662377267 40.0 38.0 41.0 35.0 41.0 26-27 38.70049067667196 40.0 38.0 41.0 34.5 41.0 28-29 38.615093126025116 40.0 38.0 41.0 34.0 41.0 30-31 38.49823069408957 40.0 38.0 41.0 34.0 41.0 32-33 38.34463844650147 40.0 38.0 41.0 34.0 41.0 34-35 38.23899126080698 40.0 38.0 41.0 34.0 41.0 36-37 38.09428966369455 40.0 38.0 41.0 33.5 41.0 38-39 37.994597908879484 40.0 38.0 41.0 33.0 41.0 40-41 37.86957846885932 40.0 37.0 41.0 33.0 41.0 42-43 37.73116316892561 40.0 37.0 41.0 33.0 41.0 44-45 37.54766319175799 40.0 37.0 41.0 33.0 41.0 46-47 37.29966248712327 40.0 37.0 41.0 32.0 41.0 48-49 37.064110952015774 40.0 37.0 41.0 31.0 41.0 50-51 36.854645039451256 40.0 36.5 41.0 31.0 41.0 52-53 36.75203252825243 40.0 36.0 41.0 31.0 41.0 54-55 36.62601057634602 40.0 36.0 41.0 31.0 41.0 56-57 36.45911599220156 39.0 35.5 41.0 30.5 41.0 58-59 36.29492968522362 39.0 35.0 41.0 30.0 41.0 60-61 36.11096290381005 39.0 35.0 41.0 30.0 41.0 62-63 35.91849646618216 39.0 35.0 41.0 30.0 41.0 64-65 35.64465276345676 38.0 35.0 40.0 29.0 41.0 66-67 35.39854365200665 38.0 35.0 40.0 29.0 41.0 68-69 35.09543810776978 37.0 35.0 40.0 29.0 41.0 70-71 34.802947017677454 37.0 34.0 39.5 29.0 41.0 72-73 34.483055534023244 36.0 34.0 39.0 28.0 41.0 74-75 34.14966394156992 36.0 34.0 39.0 28.0 41.0 76-77 33.85392789162276 35.0 34.0 38.5 28.0 40.0 78-79 33.52203234786853 35.0 34.0 37.0 28.0 39.0 80-81 33.20693517539408 35.0 34.0 37.0 27.0 39.0 82-83 32.90384978214989 35.0 34.0 36.5 27.0 39.0 84-85 32.62262155314426 35.0 33.0 36.0 26.5 37.0 86-87 32.33901282561932 35.0 33.0 36.0 26.0 37.0 88-89 32.121692819892544 35.0 33.0 35.0 26.0 37.0 90-91 31.968187514087415 35.0 33.0 35.0 26.0 36.0 92-93 31.805563802836836 35.0 33.0 35.0 26.0 36.0 94-95 31.61923431753057 35.0 33.0 35.0 25.0 36.0 96-97 31.447017758712338 35.0 33.0 35.0 25.0 36.0 98-99 31.262275814112293 35.0 33.0 35.0 24.5 35.0 100 31.144932602242317 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31018271990154744 1101 2 0.3506559280560211 1101 3 0.4488298258933803 1101 4 0.3166307776645496 1101 5 0.2562342941498912 1101 6 0.2944969176504344 1101 7 0.25815330773606604 1101 8 0.29887995691325386 1101 9 0.305589251355471 1101 10-11 0.2904911924338194 1101 12-13 0.29870022484692527 1101 14-15 0.393118979543857 1101 16-17 0.40234388316795133 1101 18-19 0.41315723992655506 1101 20-21 0.37690134501250583 1101 22-23 0.3998411032256257 1101 24-25 0.45096710214042446 1101 26-27 0.4094815055271184 1101 28-29 0.4277581848822365 1101 30-31 0.43807538484313113 1101 32-33 0.5081560582270725 1101 34-35 0.551843942200442 1101 36-37 0.45711054085170133 1101 38-39 0.4927985076661514 1101 40-41 0.5516972716141169 1101 42-43 0.48915095309587286 1101 44-45 0.5021517392745452 1101 46-47 0.5797405446090167 1101 48-49 0.5937291653277654 1101 50-51 0.6449916738066364 1101 52-53 0.6235094770001552 1101 54-55 0.5642638158166733 1101 56-57 0.5426151267939545 1101 58-59 0.5649613543413565 1101 60-61 0.649705693193873 1101 62-63 0.6493590812398295 1101 64-65 0.6189215725876807 1101 66-67 0.59512402161522 1101 68-69 0.5926108331703261 1101 70-71 0.5727328304483947 1101 72-73 0.5624420199914653 1101 74-75 0.5851828593025488 1101 76-77 0.5584908897283043 1101 78-79 0.5311379185270226 1101 80-81 0.5829189437410562 1101 82-83 0.5582596907080983 1101 84-85 0.5432886452725683 1101 86-87 0.5819462589490598 1101 88-89 0.6031320850324633 1101 90-91 0.6036015335888685 1101 92-93 0.6027642916955536 1101 94-95 0.5247900264209662 1101 96-97 0.546901688640105 1101 98-99 0.5802552339166063 1101 100 0.5951057426955586 1102 1 0.28610266302548837 1102 2 0.37326504805574956 1102 3 0.4538644345436573 1102 4 0.34971038531500653 1102 5 0.3189867342868453 1102 6 0.252677822626751 1102 7 0.2851066636063777 1102 8 0.30369824765139697 1102 9 0.3933691380132487 1102 10-11 0.317329680044665 1102 12-13 0.338789780545639 1102 14-15 0.41803681308002183 1102 16-17 0.4525064504894729 1102 18-19 0.4223672924986914 1102 20-21 0.3816903474602569 1102 22-23 0.4376377181362585 1102 24-25 0.4196839329784581 1102 26-27 0.3705918696214212 1102 28-29 0.40963357989334526 1102 30-31 0.48451075756466366 1102 32-33 0.46556225676484786 1102 34-35 0.45910314537061225 1102 36-37 0.4939150374282306 1102 38-39 0.44139349367701186 1102 40-41 0.5117639062829227 1102 42-43 0.530041332356376 1102 44-45 0.5316644070486731 1102 46-47 0.5648823456325403 1102 48-49 0.5980058788796967 1102 50-51 0.7147666428012087 1102 52-53 0.614570988503047 1102 54-55 0.5814258330999564 1102 56-57 0.5771965936623147 1102 58-59 0.5758572907607018 1102 60-61 0.5692954258686385 1102 62-63 0.5608993669716895 1102 64-65 0.5735744433386571 1102 66-67 0.6109234756205595 1102 68-69 0.5797548938927832 1102 70-71 0.6067634074235642 1102 72-73 0.6270434929085837 1102 74-75 0.6178918281387027 1102 76-77 0.6205986066596836 1102 78-79 0.5531248596884382 1102 80-81 0.5254501111903167 1102 82-83 0.5421720828537886 1102 84-85 0.5285972233766145 1102 86-87 0.5687270964030589 1102 88-89 0.5769763954046994 1102 90-91 0.5758596718561897 1102 92-93 0.6161401639049906 1102 94-95 0.584812292445605 1102 96-97 0.6297023789851934 1102 98-99 0.6843955734336689 1102 100 0.7407637363354986 1103 1 0.30159621318088625 1103 2 0.2997070771584873 1103 3 0.37792842406711813 1103 4 0.3077702711031236 1103 5 0.21598177050150014 1103 6 0.2538572155847376 1103 7 0.28815563765928687 1103 8 0.3015769898833014 1103 9 0.3166281599854983 1103 10-11 0.28809535261941477 1103 12-13 0.2476862459602529 1103 14-15 0.34777475242354683 1103 16-17 0.3849302360832212 1103 18-19 0.3971696374675844 1103 20-21 0.33059629649745403 1103 22-23 0.3722542466901615 1103 24-25 0.40026067964506495 1103 26-27 0.40310214420889423 1103 28-29 0.40245409835790014 1103 30-31 0.4203435487809699 1103 32-33 0.351106833106023 1103 34-35 0.4393434037728099 1103 36-37 0.4807477859470026 1103 38-39 0.38169469947082746 1103 40-41 0.4446810537386625 1103 42-43 0.43915264452005687 1103 44-45 0.4883566794079215 1103 46-47 0.5002307454436092 1103 48-49 0.4860484788031556 1103 50-51 0.5112471739265061 1103 52-53 0.5142347455504606 1103 54-55 0.48364626977621583 1103 56-57 0.5295521109639552 1103 58-59 0.42325758874164165 1103 60-61 0.41747225511914365 1103 62-63 0.45834673830298556 1103 64-65 0.4800961377883297 1103 66-67 0.49985324907707707 1103 68-69 0.5145417086340842 1103 70-71 0.4803081646324827 1103 72-73 0.5395194424045826 1103 74-75 0.5316389503657888 1103 76-77 0.5204474150143881 1103 78-79 0.47163554639978145 1103 80-81 0.442665452010651 1103 82-83 0.45643339855791254 1103 84-85 0.4839862799762855 1103 86-87 0.47155208749889965 1103 88-89 0.5025418922035811 1103 90-91 0.41177224908631516 1103 92-93 0.4068480967059962 1103 94-95 0.39623588735913984 1103 96-97 0.41398677521709715 1103 98-99 0.45583912877376065 1103 100 0.43484705377881383 1104 1 0.34163916035616637 1104 2 0.29191036878878407 1104 3 0.41092762885299905 1104 4 0.27954260617273263 1104 5 0.22508439547346626 1104 6 0.2531624811166111 1104 7 0.22699486995747975 1104 8 0.2582409709696023 1104 9 0.287074066673739 1104 10-11 0.3273269874975 1104 12-13 0.3080968540196949 1104 14-15 0.38168427583338627 1104 16-17 0.38858443886767446 1104 18-19 0.38927374418598504 1104 20-21 0.3827759409038265 1104 22-23 0.37164881117074344 1104 24-25 0.37502623562270543 1104 26-27 0.4036708996158751 1104 28-29 0.42028417918808003 1104 30-31 0.4119334897432694 1104 32-33 0.4089998307571179 1104 34-35 0.3889994806807522 1104 36-37 0.42917119805084525 1104 38-39 0.4555958842301635 1104 40-41 0.4619962480941524 1104 42-43 0.4283834183626425 1104 44-45 0.4436544267604532 1104 46-47 0.51517870445781 1104 48-49 0.45642635730862224 1104 50-51 0.500876607206969 1104 52-53 0.458940722973729 1104 54-55 0.43421690670523105 1104 56-57 0.44950210584877226 1104 58-59 0.49880958265750763 1104 60-61 0.4848319426869878 1104 62-63 0.5145189915805943 1104 64-65 0.5160559844500199 1104 66-67 0.4956364010486922 1104 68-69 0.4836595095162437 1104 70-71 0.4539924386035139 1104 72-73 0.4778886896350514 1104 74-75 0.47557918021506396 1104 76-77 0.4228597941282004 1104 78-79 0.42916671142035767 1104 80-81 0.44792644909231427 1104 82-83 0.4078683174126567 1104 84-85 0.43518928689084646 1104 86-87 0.43872530028897216 1104 88-89 0.3998682430279672 1104 90-91 0.43058885974953043 1104 92-93 0.37622388295897125 1104 94-95 0.42795404352368394 1104 96-97 0.4410894175373343 1104 98-99 0.4903411657924224 1104 100 0.552848563588622 1105 1 0.19493065935981235 1105 2 0.22485078544915638 1105 3 0.2867725919935289 1105 4 0.23455589088317907 1105 5 0.16210429281179728 1105 6 0.16301951940591408 1105 7 0.17716635042295792 1105 8 0.16767256713779943 1105 9 0.190765422791209 1105 10-11 0.154491905530449 1105 12-13 0.17848074608897946 1105 14-15 0.24198690748286822 1105 16-17 0.2698832239940572 1105 18-19 0.27496936763900237 1105 20-21 0.2160654345302362 1105 22-23 0.24352500361483465 1105 24-25 0.2392030648167065 1105 26-27 0.298603654906735 1105 28-29 0.2748189572539772 1105 30-31 0.2999529628578159 1105 32-33 0.30221751335975 1105 34-35 0.21283823202517738 1105 36-37 0.2590432778877698 1105 38-39 0.2722478192120832 1105 40-41 0.2796335591732415 1105 42-43 0.25216668069960235 1105 44-45 0.2523118327915981 1105 46-47 0.2065399084736299 1105 48-49 0.2037334621920479 1105 50-51 0.24181589008601634 1105 52-53 0.2540094321199575 1105 54-55 0.254129376740309 1105 56-57 0.2540785082848558 1105 58-59 0.2645402076188219 1105 60-61 0.25844061780760086 1105 62-63 0.23242429342763415 1105 64-65 0.2404054369064994 1105 66-67 0.2649138451113373 1105 68-69 0.2940370991453989 1105 70-71 0.2680712833112935 1105 72-73 0.25262594988524967 1105 74-75 0.27743610958533793 1105 76-77 0.2692013330200922 1105 78-79 0.29005863888759364 1105 80-81 0.2969577946249089 1105 82-83 0.30840440859859797 1105 84-85 0.30772054080474476 1105 86-87 0.35243106076553943 1105 88-89 0.30649370605568294 1105 90-91 0.2851186487390116 1105 92-93 0.25420262961525353 1105 94-95 0.3008911913947898 1105 96-97 0.3306089579038982 1105 98-99 0.35078621455744496 1105 100 0.3680812921248737 1106 1 0.127043296267761 1106 2 0.168445645523839 1106 3 0.26600312700665185 1106 4 0.1936618345652832 1106 5 0.152813141397111 1106 6 0.16232414682062313 1106 7 0.1185560595509898 1106 8 0.17662244352944612 1106 9 0.14162787003728283 1106 10-11 0.1873392017944937 1106 12-13 0.1562623341700018 1106 14-15 0.2541978046026401 1106 16-17 0.25751803751850133 1106 18-19 0.2413285284054396 1106 20-21 0.21490491328075478 1106 22-23 0.24780463661933538 1106 24-25 0.19179789296303795 1106 26-27 0.2289210432380102 1106 28-29 0.22692678489522677 1106 30-31 0.26928155375988894 1106 32-33 0.2885185268197361 1106 34-35 0.2605056492409119 1106 36-37 0.3014235153714253 1106 38-39 0.3610010955919378 1106 40-41 0.3237537174894811 1106 42-43 0.34451823851478025 1106 44-45 0.3323883342481082 1106 46-47 0.3116180691806818 1106 48-49 0.30046900195159765 1106 50-51 0.34409629374606254 1106 52-53 0.2921043118356863 1106 54-55 0.29463082133548824 1106 56-57 0.34913140975320545 1106 58-59 0.321116764647428 1106 60-61 0.3584648945279483 1106 62-63 0.3823686235940116 1106 64-65 0.38683847411597583 1106 66-67 0.37945080437019385 1106 68-69 0.4143764552091156 1106 70-71 0.40450896502598965 1106 72-73 0.34535067617174775 1106 74-75 0.35015720925747473 1106 76-77 0.3626018257406969 1106 78-79 0.33971132726442477 1106 80-81 0.31198877769617894 1106 82-83 0.30801828959537403 1106 84-85 0.3125713702252213 1106 86-87 0.3890695816095402 1106 88-89 0.3767098261144852 1106 90-91 0.34937385887878847 1106 92-93 0.3016651189173949 1106 94-95 0.31366042748422274 1106 96-97 0.35354996994153964 1106 98-99 0.4351310274842781 1106 100 0.44344589439530324 1107 1 0.19240643006138924 1107 2 0.20200722760559842 1107 3 0.3073972950375854 1107 4 0.21587598978392464 1107 5 0.17284351129295317 1107 6 0.17137010501456018 1107 7 0.1744703595011643 1107 8 0.19698023886482474 1107 9 0.176456920740776 1107 10-11 0.19877845013957085 1107 12-13 0.13937311311037348 1107 14-15 0.24324874535760443 1107 16-17 0.23307644025178575 1107 18-19 0.2214177399744699 1107 20-21 0.2837617714201244 1107 22-23 0.27173706360108696 1107 24-25 0.29026397595826836 1107 26-27 0.24775298857219497 1107 28-29 0.28427653695349164 1107 30-31 0.27548816395753306 1107 32-33 0.2894803983645673 1107 34-35 0.2705720833880605 1107 36-37 0.3186449071668136 1107 38-39 0.3161699095051418 1107 40-41 0.2980422789529342 1107 42-43 0.29481292141036164 1107 44-45 0.276729568499654 1107 46-47 0.3460895618136064 1107 48-49 0.34693138361577525 1107 50-51 0.41972207968575503 1107 52-53 0.3686060794585302 1107 54-55 0.3930295211243262 1107 56-57 0.4021897531702905 1107 58-59 0.3656917047380617 1107 60-61 0.37143070466864714 1107 62-63 0.33910362252631643 1107 64-65 0.3671070578823148 1107 66-67 0.38393771921586506 1107 68-69 0.42300672545373175 1107 70-71 0.40789686926591173 1107 72-73 0.38581168170001945 1107 74-75 0.33523894698495127 1107 76-77 0.3433791052623718 1107 78-79 0.289810741220343 1107 80-81 0.35186326461101913 1107 82-83 0.37165982991853497 1107 84-85 0.34441539233829843 1107 86-87 0.3676846669062428 1107 88-89 0.3585585935237887 1107 90-91 0.3159318582619015 1107 92-93 0.2834469776499198 1107 94-95 0.3042125427000144 1107 96-97 0.338550563103567 1107 98-99 0.3857735405931564 1107 100 0.40983733388091537 1108 1 0.08623621483209121 1108 2 0.12094972120892322 1108 3 0.1895449569226706 1108 4 0.1328136902227186 1108 5 0.11778023920560798 1108 6 0.09082595348572653 1108 7 0.08279726721537628 1108 8 0.12022963393041408 1108 9 0.08263755589296551 1108 10-11 0.097353004687875 1108 12-13 0.0686533279923367 1108 14-15 0.08024618668765271 1108 16-17 0.10143530239620446 1108 18-19 0.0963163772801181 1108 20-21 0.08960977947819515 1108 22-23 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-0.5465117156465666 2215 32-33 -0.6154787731142264 2215 34-35 -0.7114394706714222 2215 36-37 -0.7528994120955019 2215 38-39 -0.7185824425324867 2215 40-41 -0.7039764939266107 2215 42-43 -0.6483210185406989 2215 44-45 -0.7110497043584232 2215 46-47 -0.6619502546911917 2215 48-49 -0.6688406033163332 2215 50-51 -0.6785390629799366 2215 52-53 -0.8018260776956225 2215 54-55 -0.734119143640676 2215 56-57 -0.7007401107872724 2215 58-59 -0.723547541169097 2215 60-61 -0.8959798113215527 2215 62-63 -0.835876904422868 2215 64-65 -0.7032879739115785 2215 66-67 -0.6026258559898849 2215 68-69 -0.6198328711805061 2215 70-71 -0.7389168284327994 2215 72-73 -0.616521141924423 2215 74-75 -0.673137815223761 2215 76-77 -0.6690606881500969 2215 78-79 -0.5986317650474007 2215 80-81 -0.6623554621825392 2215 82-83 -0.6508808740126355 2215 84-85 -0.6379554300101375 2215 86-87 -0.6202496847593366 2215 88-89 -0.5704176810815014 2215 90-91 -0.5656003079219509 2215 92-93 -0.6658025265804604 2215 94-95 -0.7243690504437374 2215 96-97 -0.7765021915202013 2215 98-99 -0.7839426112576575 2215 100 -0.8616408761872414 2216 1 -0.6014666985512562 2216 2 -1.0502864498214954 2216 3 -0.681788274910371 2216 4 -1.0340109964944233 2216 5 -0.5969340658289681 2216 6 -0.7373354733733564 2216 7 -0.4761555471349084 2216 8 -0.5508506558275741 2216 9 -0.570216189668038 2216 10-11 -0.8318545932253585 2216 12-13 -0.6483152745526013 2216 14-15 -0.8900962032605264 2216 16-17 -0.8977276053426024 2216 18-19 -0.9035937227498181 2216 20-21 -0.7147548594853319 2216 22-23 -0.7366757227278384 2216 24-25 -0.8011601582867058 2216 26-27 -0.9607039420448373 2216 28-29 -1.1912523127557861 2216 30-31 -1.2919610243429958 2216 32-33 -0.991311938857244 2216 34-35 -0.9538556414589721 2216 36-37 -0.9361312762243585 2216 38-39 -1.11866542081939 2216 40-41 -0.948710369781935 2216 42-43 -1.5791057943507951 2216 44-45 -1.2701154543301172 2216 46-47 -1.1002323543050068 2216 48-49 -1.0487595060395378 2216 50-51 -1.253823798879182 2216 52-53 -1.1440787010534308 2216 54-55 -1.3369032746788605 2216 56-57 -1.4129692278082544 2216 58-59 -1.2158173152080636 2216 60-61 -1.3793833426892306 2216 62-63 -1.3884148903035012 2216 64-65 -1.3551368124174559 2216 66-67 -1.204476354827186 2216 68-69 -1.552476169927509 2216 70-71 -1.3040773619713448 2216 72-73 -1.2419145978559953 2216 74-75 -1.278526861774509 2216 76-77 -1.3556463123526186 2216 78-79 -1.3969711474166928 2216 80-81 -1.2212592352713258 2216 82-83 -1.3040339454575829 2216 84-85 -1.2656636650342747 2216 86-87 -1.4543185559991372 2216 88-89 -1.297363965727861 2216 90-91 -1.1862639477204375 2216 92-93 -1.200303269937681 2216 94-95 -1.2935061346883003 2216 96-97 -1.3724204716031707 2216 98-99 -1.4400149309005243 2216 100 -1.4583299593181458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 416.0 3 41.0 4 61.0 5 136.0 6 340.0 7 896.0 8 2088.0 9 3592.0 10 5121.0 11 7453.0 12 10849.0 13 15133.0 14 18279.0 15 23283.0 16 29184.0 17 40814.0 18 42608.0 19 23574.0 20 16698.0 21 16163.0 22 16793.0 23 18303.0 24 21723.0 25 26598.0 26 33792.0 27 43753.0 28 56951.0 29 74154.0 30 96642.0 31 125160.0 32 161328.0 33 209791.0 34 283562.0 35 409213.0 36 661079.0 37 1211250.0 38 1730868.0 39 713714.0 40 2140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.94325414370301 21.07998483841546 15.313736990737677 22.663024027143855 2 15.548074021361103 21.04219681707432 39.47531827712947 23.934410884435106 3 17.548638239791288 25.825609734099526 31.497577899431274 25.128174126677916 4 12.571287625516009 18.38306356353848 38.84084677626668 30.204802034678828 5 12.433471890563515 37.44357197484193 35.624370471416356 14.498585663178195 6 29.121492107266743 38.92699130324558 17.819579113813482 14.131937475674194 7 25.488990651401966 33.61294460768374 22.842239015799514 18.055825725114783 8 24.669154014199624 35.509364280057845 20.854197329895964 18.967284375846567 9 24.34121243076545 16.946800857197786 22.41721389918874 36.29477281284802 10-11 23.222597128191026 27.314597460357394 28.97323541900983 20.48956999244175 12-13 24.3756645561752 24.87989439579767 29.206223146567755 21.53821790145937 14-15 21.7081578144878 26.971712143324293 27.192333065438824 24.12779697674908 16-17 20.2680553300757 30.250694599842724 27.891330246656278 21.5899198234253 18-19 20.092774195288797 28.255624442699105 31.133698098802594 20.517903263209504 20-21 21.2941065009215 27.516944303468748 30.903277997732364 20.285671197877384 22-23 20.94959765455446 27.442011862109357 31.049063929511828 20.55932655382436 24-25 20.76966066540853 27.46444609227562 31.04318926511117 20.722703977204677 26-27 20.59179565333337 27.390968097565903 31.072871352324995 20.944364896775728 28-29 20.669119729196197 27.41315658932491 31.167219804234396 20.750503877244494 30-31 21.009754571566706 27.760909397459184 30.709008251368118 20.520327779606 32-33 20.56215753463615 27.552009883088473 31.12639842224202 20.75943416003336 34-35 20.766816775311394 27.28299777867807 31.034560090016434 20.9156253559941 36-37 21.165050768313474 27.507786977355973 30.459598640978054 20.8675636133525 38-39 20.957893688237817 27.58481577198697 30.641233838781982 20.816056700993233 40-41 20.81066956060923 27.407860804742896 30.749033524263993 21.032436110383887 42-43 20.813968473121907 27.43091256533025 30.539853219519227 21.215265742028617 44-45 21.062459464409365 27.58513266259779 30.284569393599753 21.067838479393092 46-47 21.091873413414028 27.80305589804118 29.853744420084492 21.2513262684603 48-49 21.076979554705314 27.646739447502032 29.74086635942903 21.535414638363623 50-51 21.101168871331524 27.667085449796968 29.46825105471758 21.76349462415392 52-53 21.057917365654223 27.797498124251348 29.433254955722255 21.71132955437217 54-55 21.13250854020196 28.123229170577012 29.258412592550343 21.485849696670684 56-57 20.911887671866435 27.804372212886136 29.249840295257545 22.033899819989884 58-59 20.772847078255694 27.463020024431493 29.604960272415866 22.15917262489695 60-61 21.01607155422494 27.706599271346604 29.29340056614539 21.983928608283065 62-63 20.78170250861983 27.67694968573389 29.37380140189156 22.167546403754717 64-65 20.673805318334495 27.51947130295506 29.560620605072558 22.246102773637887 66-67 20.60167614007085 27.524517176527407 29.355454248064895 22.518352435336848 68-69 21.044682798566843 27.67386428224068 29.067443671673676 22.214009247518806 70-71 20.913764639330545 27.388156709069882 29.108214568420177 22.589864083179396 72-73 20.6456670571734 27.452046731452107 29.322156357727575 22.580129853646916 74-75 20.728700522609493 27.47717859451051 29.09076933402432 22.703351548855675 76-77 20.73888977455752 27.551087235434935 28.86900278424966 22.841020205757886 78-79 20.80916598568936 27.54211056135471 28.94132290008659 22.707400552869338 80-81 20.63123143595279 27.366230182606643 28.973454656698944 23.02908372474162 82-83 20.461637841724865 27.317659390349437 29.072409716382076 23.14829305154363 84-85 20.490853805685603 27.22284544042351 28.8808496178543 23.40545113603659 86-87 20.616643127382062 27.292894516216105 28.772009881136768 23.318452475265065 88-89 20.452854816321565 27.13390399135327 28.83900395861601 23.57423723370915 90-91 20.265907147090477 27.003989086605284 28.780925902964132 23.949177863340104 92-93 20.348538173634815 27.071580877292455 28.636194257545423 23.94368669152731 94-95 20.214049002213784 26.819105719958447 28.851681503080158 24.115163774747614 96-97 20.19423606855433 26.79062289806052 28.82818077325534 24.186960260129815 98-99 20.18296457275575 26.768116113957802 28.729295863958654 24.319623449327793 100 20.2508238017396 26.74629961600609 28.596503801180567 24.406372781073742 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 810.0 1 620.5 2 1039.5 3 1601.5 4 1008.0 5 560.5 6 1305.5 7 2982.0 8 5344.5 9 6788.5 10 5434.0 11 3611.5 12 3387.0 13 3768.0 14 4203.5 15 4754.5 16 5441.5 17 6179.5 18 6994.5 19 7906.5 20 9296.5 21 11234.5 22 14047.5 23 18701.5 24 24470.5 25 31710.5 26 42355.5 27 56010.0 28 74814.5 29 95534.0 30 113727.5 31 131034.5 32 147329.0 33 164194.0 34 179890.0 35 192519.0 36 202433.0 37 210447.0 38 217394.5 39 221416.0 40 224887.5 41 225323.0 42 220589.0 43 216731.0 44 213153.5 45 208255.5 46 203638.5 47 198801.0 48 193482.0 49 190465.0 50 185550.0 51 175636.5 52 163655.0 53 154552.5 54 149248.0 55 141943.5 56 131127.5 57 117979.5 58 106262.0 59 95828.0 60 82156.0 61 67433.0 62 55154.0 63 44616.0 64 35723.0 65 28340.0 66 22310.0 67 17168.0 68 13054.5 69 10000.0 70 7565.0 71 5687.5 72 4344.5 73 3298.0 74 2466.5 75 1768.5 76 1187.0 77 808.5 78 558.5 79 381.0 80 241.0 81 118.5 82 61.0 83 39.5 84 21.0 85 12.0 86 6.5 87 5.0 88 3.0 89 1.5 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061590534103686284 2 3.5751761221137156E-4 3 0.0 4 0.11887460606028104 5 0.002096353271603042 6 0.0015925784543961098 7 0.0 8 0.0 9 6.500320222024938E-5 10-11 0.0 12-13 0.0 14-15 2.4376200832593514E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.7063340582815461E-4 30-31 2.925144099911222E-4 32-33 1.6250800555062344E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6250800555062345E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.125400277531172E-6 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.5438260527309228E-4 80-81 2.518874086034663E-4 82-83 2.925144099911222E-4 84-85 0.0 86-87 0.0 88-89 5.687780194271821E-5 90-91 6.500320222024938E-5 92-93 6.825336233126185E-4 94-95 7.394114252553366E-4 96-97 0.0 98-99 3.250160111012469E-5 100 9.750480333037407E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6153543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.34977638161443 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50685198307072 46.936782608226636 2 14.403669161222943 17.673237642315666 3 4.285858916869081 7.888094584591991 4 1.7416778798123242 4.2740619382116165 5 0.8755135069674855 2.685627893576913 6 0.5222325530548841 1.9223310209470041 7 0.3379861513059008 1.451476236188966 8 0.23582755146442894 1.1574374037573105 9 0.16942896741956348 0.935498633738225 >10 0.8549509325568135 9.173939738676973 >50 0.04309194817277259 1.7897649306416055 >100 0.0213990326556076 2.4193851001475726 >500 8.485314032518477E-4 0.34516863640132034 >1k 5.568225805966455E-4 0.4973832976961805 >5k 5.303072180465515E-5 0.23421391630388555 >10k+ 5.303072180465515E-5 0.6155964185781317 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18974 0.3083426897317529 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18869 0.30663635567347136 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8470 0.13764428070137805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.6250800555062344E-5 0.0 3 0.0 0.0 0.0 1.6250800555062344E-5 0.0 4 0.0 0.0 0.0 6.500320222024938E-5 0.0 5 0.0 0.0 0.0 6.500320222024938E-5 0.0 6 0.0 0.0 0.0 6.500320222024938E-5 0.0 7 0.0 0.0 0.0 6.500320222024938E-5 0.0 8 0.0 0.0 0.0 8.125400277531172E-5 0.0 9 1.6250800555062344E-5 0.0 0.0 1.137556038854364E-4 0.0 10-11 1.6250800555062344E-5 8.125400277531172E-6 0.0 1.137556038854364E-4 0.0 12-13 1.6250800555062344E-5 1.6250800555062344E-5 0.0 1.137556038854364E-4 0.0 14-15 1.6250800555062344E-5 8.937940305284289E-5 0.0 1.3813180471802992E-4 0.0 16-17 1.6250800555062344E-5 1.6250800555062345E-4 0.0 1.462572049955611E-4 0.0 18-19 1.6250800555062344E-5 1.8688420638321695E-4 0.0 1.5438260527309228E-4 0.0 20-21 1.6250800555062344E-5 2.0313500693827928E-4 0.0 2.518874086034663E-4 0.0 22-23 1.6250800555062344E-5 2.1938580749334164E-4 0.0 7.06909824145212E-4 0.0 24-25 1.6250800555062344E-5 2.3563660804840398E-4 0.0 0.0019500960666074812 0.0 26-27 1.6250800555062344E-5 2.7626360943605984E-4 0.0 0.003843314331272244 0.0 28-29 1.6250800555062344E-5 2.925144099911222E-4 0.0 0.010010493141918403 0.0 30-31 1.6250800555062344E-5 2.925144099911222E-4 0.0 0.026513181105584212 0.0 32-33 1.6250800555062344E-5 3.087652105461845E-4 0.0 0.0667582886801961 0.0 34-35 3.250160111012469E-5 3.250160111012469E-4 0.0 0.13278529133541442 0.0 36-37 3.250160111012469E-5 3.981446135990274E-4 0.0 0.23943116997801106 0.0 38-39 3.250160111012469E-5 4.550224155417456E-4 0.0 0.3906286183423111 0.0 40-41 3.250160111012469E-5 4.550224155417456E-4 0.0 0.5568824334208764 0.0 42-43 3.250160111012469E-5 4.7127321609680795E-4 0.0 0.7352674711137956 0.0 44-45 4.875240166518703E-5 4.7127321609680795E-4 0.0 0.9617548784496996 0.0 46-47 6.500320222024938E-5 5.037748172069327E-4 0.0 1.2367346746419094 0.0 48-49 6.500320222024938E-5 5.20025617761995E-4 0.0 1.5303703898713312 0.0 50-51 6.500320222024938E-5 5.525272188721197E-4 0.0 1.8280606798392407 0.0 52-53 6.500320222024938E-5 5.525272188721197E-4 0.0 2.125612838002432 0.0 54-55 6.500320222024938E-5 5.525272188721197E-4 0.0 2.4654820808110056 0.0 56-57 6.500320222024938E-5 5.525272188721197E-4 0.0 2.868298799569614 0.0 58-59 6.500320222024938E-5 5.68778019427182E-4 0.0 3.303990887851113 0.0 60-61 6.500320222024938E-5 5.68778019427182E-4 0.0 3.747044588784055 0.0 62-63 6.500320222024938E-5 5.68778019427182E-4 0.0 4.182200400647236 0.0 64-65 6.500320222024938E-5 5.68778019427182E-4 0.0 4.651726655684376 0.0 66-67 6.500320222024938E-5 5.850288199822443E-4 0.0 5.194714654630674 0.0 68-69 6.500320222024938E-5 5.850288199822443E-4 0.0 5.78019849702846 0.0 70-71 6.500320222024938E-5 5.850288199822443E-4 0.0 6.395543185446173 0.0 72-73 6.500320222024938E-5 6.337812216474315E-4 0.0 7.026057996182036 0.0 74-75 9.750480333037406E-5 6.500320222024938E-4 0.0 7.649812473886994 0.0 76-77 9.750480333037406E-5 6.825336233126185E-4 0.0 8.30783988996258 0.0 78-79 9.750480333037406E-5 6.825336233126185E-4 0.0 9.033080617133901 0.0 80-81 9.750480333037406E-5 6.906590235901496E-4 0.0 9.792699912879458 0.0 82-83 9.750480333037406E-5 6.987844238676808E-4 0.0 10.547411466857387 0.0 84-85 9.750480333037406E-5 7.475368255328678E-4 0.0 11.287546052737422 0.0 86-87 9.750480333037406E-5 7.637876260879301E-4 0.0 12.045767779635245 0.0 88 9.750480333037406E-5 7.800384266429925E-4 0.0 12.643967873467366 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12705 0.0 55.740414 1 GTATCAA 22980 0.0 40.160866 1 ATCAACG 27730 0.0 32.98943 3 TCAACGC 28020 0.0 32.66479 4 GTGGTAT 5330 0.0 32.598392 1 TATCAAC 28750 0.0 32.0318 2 CAACGCA 29080 0.0 31.500406 5 AACGCAG 30325 0.0 30.3459 6 TGGTATC 5560 0.0 29.622498 2 CGCAGAG 34140 0.0 26.554565 8 ACGCAGA 34450 0.0 26.479319 7 GCAGAGT 36725 0.0 24.442297 9 GAGTACT 25490 0.0 22.816336 12-13 CAGAGTA 35900 0.0 21.678856 10-11 GTACATG 17430 0.0 20.774279 1 GTACTTT 27820 0.0 20.753366 14-15 AGAGTAC 33995 0.0 20.377594 10-11 TACATGG 17750 0.0 19.19414 2 AGTACTT 26765 0.0 18.919973 12-13 ACATGGG 17745 0.0 18.350948 3 >>END_MODULE