FastQCFastQC Report
Wed 25 May 2016
SRR2049489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013028
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59400.042.2258451
GTATCAA92850.035.3382341
ATCAACG99400.031.3546223
TCAACGC99400.031.148684
CAACGCA108250.028.6491225
TATCAAC113700.027.6362152
AACGCAG114850.027.0470666
ACGCAGA132850.023.1504297
CGCAGAG136550.022.826268
GTACATG146650.022.5136741
GTGGTAT36800.021.9774841
TACATGG145850.021.298692
ACATGGG151800.019.7895983
TCTGTCG17100.019.31238
GCAGAGT158650.018.7626029
GTCTCGC15700.017.6060871
TGGTATC42800.017.4574092
AGAGTAC138500.016.9521510-11
TAGACCG2453.8789512E-516.7105395
CATGGGG112450.016.7023374
Produced by FastQC (version 0.11.5)