FastQCFastQC Report
Wed 25 May 2016
SRR2049489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013028
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193650.32205072053547734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189040.314384034133884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113010.18794191545424369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115800.056.0297161
GTATCAA205850.039.1589161
GTGGTAT47800.036.1506351
TGGTATC52000.031.8534622
ATCAACG258500.030.7458463
TCAACGC262050.030.4191154
TATCAAC264550.030.1138692
CAACGCA272350.029.3712165
AACGCAG285600.028.1233086
ACGCAGA319100.025.0669147
CGCAGAG321600.024.828218
GTACATG145300.022.9103411
GCAGAGT345700.022.8118579
TATAACG6250.021.83412
ATAACGC6300.021.6608163
GAGTACT264000.021.16635112-13
TACATGG150200.020.9591432
AGAGTAC324450.020.5180910-11
CAGAGTA342450.020.40712710-11
ACATGGG150700.019.7967243
Produced by FastQC (version 0.11.5)