##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049489_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6013028 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.821034759858094 31.0 31.0 34.0 30.0 34.0 2 31.984267992765044 33.0 31.0 34.0 30.0 34.0 3 31.943330880880648 33.0 31.0 34.0 30.0 34.0 4 35.61716858793939 37.0 35.0 37.0 33.0 37.0 5 35.74046753149994 37.0 35.0 37.0 35.0 37.0 6 35.79223911812817 37.0 35.0 37.0 35.0 37.0 7 35.80280999855647 37.0 35.0 37.0 35.0 37.0 8 35.79342720506207 37.0 35.0 37.0 35.0 37.0 9 37.53681722420052 39.0 37.0 39.0 35.0 39.0 10-11 37.491303466406606 39.0 37.0 39.0 34.5 39.0 12-13 37.471802891987195 39.0 37.0 39.0 34.5 39.0 14-15 38.7438536790449 40.0 38.0 41.0 34.5 41.0 16-17 38.74919940502522 40.0 38.0 41.0 35.0 41.0 18-19 38.82283335450958 40.0 38.0 41.0 35.0 41.0 20-21 38.84577113228144 40.0 38.0 41.0 35.0 41.0 22-23 38.8101573782793 40.0 38.0 41.0 35.0 41.0 24-25 38.76362541468292 40.0 38.0 41.0 34.5 41.0 26-27 38.66812220066163 40.0 38.0 41.0 34.0 41.0 28-29 38.574349562317025 40.0 38.0 41.0 34.0 41.0 30-31 38.44553991765879 40.0 38.0 41.0 34.0 41.0 32-33 38.28181325614982 40.0 38.0 41.0 34.0 41.0 34-35 38.16334324403611 40.0 38.0 41.0 34.0 41.0 36-37 38.01428132381888 40.0 37.5 41.0 33.0 41.0 38-39 37.90696650672506 40.0 37.0 41.0 33.0 41.0 40-41 37.77485220424718 40.0 37.0 41.0 33.0 41.0 42-43 37.625372524458555 40.0 37.0 41.0 33.0 41.0 44-45 37.43430472966366 40.0 37.0 41.0 32.5 41.0 46-47 37.179260848278105 40.0 37.0 41.0 32.0 41.0 48-49 36.941491790824855 40.0 37.0 41.0 31.0 41.0 50-51 36.72917130936361 40.0 36.0 41.0 31.0 41.0 52-53 36.62065618187708 40.0 36.0 41.0 31.0 41.0 54-55 36.48712088152591 39.5 36.0 41.0 30.5 41.0 56-57 36.31471789254931 39.0 35.0 41.0 30.0 41.0 58-59 36.141498592722336 39.0 35.0 41.0 30.0 41.0 60-61 35.947718603671895 39.0 35.0 41.0 30.0 41.0 62-63 35.73641192091572 38.5 35.0 41.0 29.0 41.0 64-65 35.45065630826931 38.0 35.0 40.0 29.0 41.0 66-67 35.19603068537183 37.5 35.0 40.0 29.0 41.0 68-69 34.8812623190845 37.0 34.0 40.0 28.0 41.0 70-71 34.57622332708246 36.5 34.0 39.0 28.0 41.0 72-73 34.25168650470279 36.0 34.0 39.0 28.0 41.0 74-75 33.91798549748978 35.5 34.0 39.0 28.0 40.5 76-77 33.625250040412254 35.0 34.0 37.5 27.5 40.0 78-79 33.29789990999544 35.0 34.0 37.0 27.0 39.0 80-81 32.990181735391886 35.0 33.5 37.0 26.5 39.0 82-83 32.69685988490325 35.0 33.0 36.0 26.0 39.0 84-85 32.42560811291749 35.0 33.0 36.0 26.0 37.0 86-87 32.14705269957166 35.0 33.0 35.5 26.0 37.0 88-89 31.93913640515228 35.0 33.0 35.0 25.5 36.5 90-91 31.787235732146932 35.0 33.0 35.0 25.0 36.0 92-93 31.6227785734575 35.0 33.0 35.0 25.0 36.0 94-95 31.435603409796194 35.0 33.0 35.0 25.0 36.0 96-97 31.263569619166915 35.0 33.0 35.0 24.0 35.5 98-99 31.07728535440048 35.0 33.0 35.0 23.5 35.0 100 30.96196791367012 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37824865969476207 1101 2 0.42422461286741253 1101 3 0.48813469402895393 1101 4 0.3709534987726073 1101 5 0.30332564179985866 1101 6 0.26730539152567445 1101 7 0.30325893833480677 1101 8 0.32881997297891274 1101 9 0.36315266598206364 1101 10-11 0.3778112763330128 1101 12-13 0.3550796107064471 1101 14-15 0.49119310462432964 1101 16-17 0.5322976300051963 1101 18-19 0.49843618679253865 1101 20-21 0.47812331090436544 1101 22-23 0.5345883191200116 1101 24-25 0.5535419098306136 1101 26-27 0.5722099545966088 1101 28-29 0.5258239741870057 1101 30-31 0.5373841887415907 1101 32-33 0.5270890233025511 1101 34-35 0.5312318580035225 1101 36-37 0.5533329496858812 1101 38-39 0.5792730362600551 1101 40-41 0.6021266499758298 1101 42-43 0.5558554145573993 1101 44-45 0.5676675575700756 1101 46-47 0.6048055488375113 1101 48-49 0.5735783934673506 1101 50-51 0.5885566343501054 1101 52-53 0.5856348919947791 1101 54-55 0.5598597557129565 1101 56-57 0.499509080919708 1101 58-59 0.5007027154822339 1101 60-61 0.5577108575954668 1101 62-63 0.56703252284575 1101 64-65 0.5422727365177451 1101 66-67 0.5281291593127406 1101 68-69 0.4792316359503346 1101 70-71 0.4544256253395531 1101 72-73 0.424177662848102 1101 74-75 0.39659027918159495 1101 76-77 0.42671292314975773 1101 78-79 0.4107420822728045 1101 80-81 0.4161596262712379 1101 82-83 0.4342621945272498 1101 84-85 0.45243900018576966 1101 86-87 0.5464370756758825 1101 88-89 0.5527107680017558 1101 90-91 0.5665915893670643 1101 92-93 0.5481895309015421 1101 94-95 0.4768510162477213 1101 96-97 0.504140681202955 1101 98-99 0.5771482753933732 1101 100 0.6177579485169851 1102 1 0.27807578371096753 1102 2 0.3158948438122593 1102 3 0.42038827187031913 1102 4 0.3611917196945811 1102 5 0.3015695420800455 1102 6 0.28964786911294027 1102 7 0.31550652120100153 1102 8 0.3183902010995112 1102 9 0.3651528593120048 1102 10-11 0.332750405061347 1102 12-13 0.29171850997194326 1102 14-15 0.444223660654643 1102 16-17 0.43307482947540876 1102 18-19 0.41742703215391685 1102 20-21 0.40238845331195705 1102 22-23 0.37667779798226064 1102 24-25 0.38329075851295613 1102 26-27 0.3296515123235011 1102 28-29 0.3657642369817964 1102 30-31 0.4639336161326568 1102 32-33 0.4970116520190899 1102 34-35 0.46140038406361583 1102 36-37 0.4865904602193041 1102 38-39 0.4340912682635505 1102 40-41 0.45013166840185903 1102 42-43 0.5342539962609862 1102 44-45 0.5445021684629978 1102 46-47 0.49807145786819973 1102 48-49 0.5751383244392443 1102 50-51 0.5632029191687806 1102 52-53 0.5744429299200604 1102 54-55 0.5069494194615274 1102 56-57 0.5223086644450774 1102 58-59 0.5555965397843323 1102 60-61 0.5386778450520211 1102 62-63 0.454167674451071 1102 64-65 0.5453537213823978 1102 66-67 0.6027643655104811 1102 68-69 0.5278069995430954 1102 70-71 0.5527608842080625 1102 72-73 0.5847143339568888 1102 74-75 0.5690848730766191 1102 76-77 0.5592600990040069 1102 78-79 0.49708866462743373 1102 80-81 0.4643371567316237 1102 82-83 0.46430470673338675 1102 84-85 0.4989590321085018 1102 86-87 0.5151963764038072 1102 88-89 0.49167197768817417 1102 90-91 0.4970210890122857 1102 92-93 0.562450518462331 1102 94-95 0.5642172611891851 1102 96-97 0.5623907671678126 1102 98-99 0.5821464512787848 1102 100 0.6843757925794982 1103 1 0.2783440787916831 1103 2 0.30521288942658487 1103 3 0.3839408530961599 1103 4 0.3187375847955565 1103 5 0.23883325995392113 1103 6 0.22579930517617441 1103 7 0.24399087199688552 1103 8 0.25237866949311893 1103 9 0.305111386185672 1103 10-11 0.2611587190151923 1103 12-13 0.23423789270162843 1103 14-15 0.3139645105454747 1103 16-17 0.3498111410387281 1103 18-19 0.3229810680217611 1103 20-21 0.340292832956294 1103 22-23 0.3484537581209608 1103 24-25 0.34266486810709296 1103 26-27 0.3624397761007998 1103 28-29 0.3994225088626564 1103 30-31 0.406804287406338 1103 32-33 0.3923510351597983 1103 34-35 0.4030298608090632 1103 36-37 0.48031281077414434 1103 38-39 0.41250659438050974 1103 40-41 0.40496309044632284 1103 42-43 0.39033524959697274 1103 44-45 0.39682686021941294 1103 46-47 0.41914203605367817 1103 48-49 0.42361525483915585 1103 50-51 0.4004197458053369 1103 52-53 0.30591528144687885 1103 54-55 0.2739376412491623 1103 56-57 0.34548182238454217 1103 58-59 0.3364818830026337 1103 60-61 0.3684981726047454 1103 62-63 0.3382002014458152 1103 64-65 0.370827354815944 1103 66-67 0.3215994938650084 1103 68-69 0.3490950519835323 1103 70-71 0.3696273859523487 1103 72-73 0.3540563236783143 1103 74-75 0.33057546178173425 1103 76-77 0.38861377751535287 1103 78-79 0.3726420423239176 1103 80-81 0.2949350706894691 1103 82-83 0.30936614439067256 1103 84-85 0.3270256246082397 1103 86-87 0.40233416693646973 1103 88-89 0.39375057723284 1103 90-91 0.36385018329630725 1103 92-93 0.3471478262915646 1103 94-95 0.3855249199154045 1103 96-97 0.42303496695642195 1103 98-99 0.44931555910391197 1103 100 0.4373316762148036 1104 1 0.264455925736641 1104 2 0.23570768539495646 1104 3 0.3513549811095835 1104 4 0.24500149344463296 1104 5 0.1676696113939542 1104 6 0.20320945889290698 1104 7 0.13749821872963253 1104 8 0.18535796985918296 1104 9 0.23174705485539704 1104 10-11 0.18761470014646164 1104 12-13 0.22694877111223377 1104 14-15 0.3257353145649091 1104 16-17 0.336381773404959 1104 18-19 0.3029555005227351 1104 20-21 0.304307029277453 1104 22-23 0.32603751275849646 1104 24-25 0.3470752709517342 1104 26-27 0.35214129842739794 1104 28-29 0.33897839959972487 1104 30-31 0.3952061316197373 1104 32-33 0.3947649612562927 1104 34-35 0.3812733121774343 1104 36-37 0.39777865915946364 1104 38-39 0.4073310640019159 1104 40-41 0.38106800870330915 1104 42-43 0.41137826139511446 1104 44-45 0.4328763881387019 1104 46-47 0.47399373455609606 1104 48-49 0.46611945553155465 1104 50-51 0.4641762585523779 1104 52-53 0.5136289419710209 1104 54-55 0.4452998379232369 1104 56-57 0.40284209388791226 1104 58-59 0.4286389374268964 1104 60-61 0.3345821364896153 1104 62-63 0.4044632627318663 1104 64-65 0.4359072040943559 1104 66-67 0.4698221962184448 1104 68-69 0.41044357970216794 1104 70-71 0.4107908900213104 1104 72-73 0.4276760680096743 1104 74-75 0.44744636710894525 1104 76-77 0.4085183668726913 1104 78-79 0.3561319860936294 1104 80-81 0.39007964876486056 1104 82-83 0.39443156150472447 1104 84-85 0.42369126166619253 1104 86-87 0.4267007895342658 1104 88-89 0.3836256475531208 1104 90-91 0.34037334480830594 1104 92-93 0.3442798385183643 1104 94-95 0.340486053156809 1104 96-97 0.3469899568952428 1104 98-99 0.40664278556615585 1104 100 0.4715447128077095 1105 1 0.22206810995800907 1105 2 0.21870874787123284 1105 3 0.33763027536958035 1105 4 0.24512036695674055 1105 5 0.1802658534127346 1105 6 0.20275063242841185 1105 7 0.20463613203513376 1105 8 0.19280946744286354 1105 9 0.21607444221996275 1105 10-11 0.19932989974874715 1105 12-13 0.1725430455973509 1105 14-15 0.2702789874792302 1105 16-17 0.2511723799896757 1105 18-19 0.28190303946670525 1105 20-21 0.22819277473476518 1105 22-23 0.2820151861883744 1105 24-25 0.2401089644600063 1105 26-27 0.25105921813890575 1105 28-29 0.30621682172431974 1105 30-31 0.3129165625225525 1105 32-33 0.3556354937367914 1105 34-35 0.30483525377542975 1105 36-37 0.32655751658975873 1105 38-39 0.3489131677615944 1105 40-41 0.32279501168657987 1105 42-43 0.3548802861904079 1105 44-45 0.4071425743607904 1105 46-47 0.3957310056815757 1105 48-49 0.3975761158677429 1105 50-51 0.4348033977697554 1105 52-53 0.44094436529831427 1105 54-55 0.49913823327852214 1105 56-57 0.5055956116333533 1105 58-59 0.4614122635462081 1105 60-61 0.5123679827718917 1105 62-63 0.4811775755883332 1105 64-65 0.46644749128871155 1105 66-67 0.4974815206162617 1105 68-69 0.5097392819643929 1105 70-71 0.49178416876117836 1105 72-73 0.49630331466846656 1105 74-75 0.4953989554962064 1105 76-77 0.47326252173564143 1105 78-79 0.4438723609812314 1105 80-81 0.43967659926978087 1105 82-83 0.47859378044727663 1105 84-85 0.4977562127368458 1105 86-87 0.527802623059543 1105 88-89 0.48000982135085124 1105 90-91 0.4686042889290505 1105 92-93 0.46898501956221494 1105 94-95 0.5193719093941169 1105 96-97 0.6011330275912954 1105 98-99 0.6173642582501344 1105 100 0.5966522048133385 1106 1 0.17427302231312325 1106 2 0.15970329450912857 1106 3 0.26938227070374765 1106 4 0.20199084437765435 1106 5 0.12065609641349795 1106 6 0.1755445404815461 1106 7 0.17612071756794023 1106 8 0.18913624305678667 1106 9 0.1701127928150612 1106 10-11 0.20207555763503393 1106 12-13 0.15107802669469095 1106 14-15 0.25363993987917866 1106 16-17 0.2528716001457667 1106 18-19 0.223026848687077 1106 20-21 0.24502547603392344 1106 22-23 0.22264469174243118 1106 24-25 0.19142489026198461 1106 26-27 0.24282785058103684 1106 28-29 0.24535002730502242 1106 30-31 0.23363764209465643 1106 32-33 0.2603038314512318 1106 34-35 0.24131650604949328 1106 36-37 0.27535259401962264 1106 38-39 0.2707340403092573 1106 40-41 0.25194806845875206 1106 42-43 0.31315935424969155 1106 44-45 0.28525702491911176 1106 46-47 0.2567995406918655 1106 48-49 0.25754136168979613 1106 50-51 0.32825643250666303 1106 52-53 0.3268323376457616 1106 54-55 0.2449294377560136 1106 56-57 0.2595716810001534 1106 58-59 0.22523773552883597 1106 60-61 0.2943806496415675 1106 62-63 0.3384723533775684 1106 64-65 0.29858980380415545 1106 66-67 0.26743039321841167 1106 68-69 0.29344875508331114 1106 70-71 0.30481082607542476 1106 72-73 0.28709379193372087 1106 74-75 0.3182943084397891 1106 76-77 0.3315960455872329 1106 78-79 0.2458282761109416 1106 80-81 0.23551707298410207 1106 82-83 0.24373162729589382 1106 84-85 0.2638557308796976 1106 86-87 0.3121831683568388 1106 88-89 0.31704566415820423 1106 90-91 0.29342296621999253 1106 92-93 0.31569795338444706 1106 94-95 0.3071574739637519 1106 96-97 0.28530908397042865 1106 98-99 0.3020451008531957 1106 100 0.33847495862743315 1107 1 0.11705543473948765 1107 2 0.14254403842299013 1107 3 0.22480910714864066 1107 4 0.13615836125371317 1107 5 0.11794311120226553 1107 6 0.13567924036870949 1107 7 0.12466438410354641 1107 8 0.1692053959822175 1107 9 0.15681768742604163 1107 10-11 0.1520935785863955 1107 12-13 0.10325840811225362 1107 14-15 0.16846535123539752 1107 16-17 0.18284357826099296 1107 18-19 0.1673365818156114 1107 20-21 0.23598115158728206 1107 22-23 0.22235680578830141 1107 24-25 0.25093156132437855 1107 26-27 0.19076448564113946 1107 28-29 0.21808003916746088 1107 30-31 0.21666416998728977 1107 32-33 0.23776642238580337 1107 34-35 0.215641092792751 1107 36-37 0.2453576855221229 1107 38-39 0.22857783599157244 1107 40-41 0.19729612996655987 1107 42-43 0.2307864989039885 1107 44-45 0.2174846633776255 1107 46-47 0.2245316041111849 1107 48-49 0.2008409934499653 1107 50-51 0.280352428462308 1107 52-53 0.274968814031638 1107 54-55 0.23801794625317285 1107 56-57 0.2345545216376621 1107 58-59 0.2381853560686693 1107 60-61 0.2466667626438337 1107 62-63 0.272264625395394 1107 64-65 0.27053650576907273 1107 66-67 0.25947690825844916 1107 68-69 0.24441714845683293 1107 70-71 0.2491114733577291 1107 72-73 0.2655669630613531 1107 74-75 0.23993706608928989 1107 76-77 0.2077222368188174 1107 78-79 0.18791175819028183 1107 80-81 0.231819831114791 1107 82-83 0.31033125259489225 1107 84-85 0.28350650689195334 1107 86-87 0.3237478072364297 1107 88-89 0.23147070713863016 1107 90-91 0.19958878581032025 1107 92-93 0.18343022961474276 1107 94-95 0.22146960298915275 1107 96-97 0.27018083077786414 1107 98-99 0.32414896667682314 1107 100 0.2981433778515701 1108 1 0.11750061956899316 1108 2 0.1486719872372788 1108 3 0.21890460359360375 1108 4 0.16544552875662077 1108 5 0.13766310390330716 1108 6 0.15341558638046848 1108 7 0.09399978570662881 1108 8 0.14074573131510704 1108 9 0.12228023512528807 1108 10-11 0.13560163713149365 1108 12-13 0.15228426127526262 1108 14-15 0.1643009637521402 1108 16-17 0.20735307605951192 1108 18-19 0.18146469938869103 1108 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-0.6202564951871281 2215 34-35 -0.6670515798614005 2215 36-37 -0.6708962848582303 2215 38-39 -0.6362879452769548 2215 40-41 -0.5908728874884588 2215 42-43 -0.5998023617224391 2215 44-45 -0.7073708906092762 2215 46-47 -0.6757049549728933 2215 48-49 -0.6335322759317066 2215 50-51 -0.6102551622382038 2215 52-53 -0.7317764457972586 2215 54-55 -0.6881904158293608 2215 56-57 -0.6819368589694932 2215 58-59 -0.6314408880414106 2215 60-61 -0.7682443944001989 2215 62-63 -0.7367711797735979 2215 64-65 -0.630559476177325 2215 66-67 -0.5051607575085413 2215 68-69 -0.4743447725525627 2215 70-71 -0.597474568506037 2215 72-73 -0.5250524665410339 2215 74-75 -0.6476820422375553 2215 76-77 -0.6268897380068452 2215 78-79 -0.5580845558683052 2215 80-81 -0.5909455476310796 2215 82-83 -0.6198886592476924 2215 84-85 -0.5898467246780186 2215 86-87 -0.4891771126621016 2215 88-89 -0.5081805537830526 2215 90-91 -0.5086436516459543 2215 92-93 -0.6369866890796025 2215 94-95 -0.6198259700756807 2215 96-97 -0.6151640303585069 2215 98-99 -0.5957484496547671 2215 100 -0.6804850142836969 2216 1 -0.5733499950464314 2216 2 -0.9891368288955427 2216 3 -0.5846495433509524 2216 4 -1.0537282069769276 2216 5 -0.542738614104394 2216 6 -0.7291129175581617 2216 7 -0.4652652051076842 2216 8 -0.5054710186486133 2216 9 -0.5435218606072709 2216 10-11 -0.7842809360699974 2216 12-13 -0.5435361479019889 2216 14-15 -0.7855122172298081 2216 16-17 -0.7860692082909111 2216 18-19 -0.7994159485037216 2216 20-21 -0.6646041542029053 2216 22-23 -0.6558217338747241 2216 24-25 -0.7267368052707894 2216 26-27 -0.8867336125365028 2216 28-29 -1.0651690433947252 2216 30-31 -1.2215899108866424 2216 32-33 -0.9147045364260649 2216 34-35 -0.9340341182556315 2216 36-37 -0.9690998926949277 2216 38-39 -1.1407586277908095 2216 40-41 -0.9247416348033397 2216 42-43 -1.5013288089950976 2216 44-45 -1.1900619039160887 2216 46-47 -1.0158753575069355 2216 48-49 -0.9984620472126338 2216 50-51 -1.218878802500221 2216 52-53 -1.1825492482152171 2216 54-55 -1.3156681202173246 2216 56-57 -1.356233866198906 2216 58-59 -1.1919621391101174 2216 60-61 -1.3952312147378905 2216 62-63 -1.3643161922074825 2216 64-65 -1.3443770397286983 2216 66-67 -1.1518841157381559 2216 68-69 -1.4523382202097892 2216 70-71 -1.2777271832140755 2216 72-73 -1.121910754453559 2216 74-75 -1.2185281223349875 2216 76-77 -1.3246946718206019 2216 78-79 -1.3739273190275156 2216 80-81 -1.180173523489124 2216 82-83 -1.2557909733502015 2216 84-85 -1.3198514313948309 2216 86-87 -1.509347646334227 2216 88-89 -1.3207654193650953 2216 90-91 -1.2224731316578072 2216 92-93 -1.276496326730829 2216 94-95 -1.3865219575069538 2216 96-97 -1.456233840576047 2216 98-99 -1.4268988470744297 2216 100 -1.4353026140405802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 353.0 3 49.0 4 61.0 5 105.0 6 373.0 7 1033.0 8 2430.0 9 3910.0 10 5727.0 11 8668.0 12 12334.0 13 16841.0 14 19659.0 15 23795.0 16 30013.0 17 41208.0 18 41772.0 19 23522.0 20 17581.0 21 16787.0 22 17475.0 23 19236.0 24 22434.0 25 27229.0 26 34696.0 27 44102.0 28 57375.0 29 75144.0 30 97395.0 31 124183.0 32 159406.0 33 206970.0 34 280930.0 35 409487.0 36 659987.0 37 1206714.0 38 1652823.0 39 649121.0 40 2100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97046512223882 20.310345527650302 14.53659676281871 23.182592587292167 2 16.100007051369893 20.628100773388926 39.319236802296885 23.952655372944292 3 17.92303312075048 25.093064592415004 30.862187902667344 26.121714384167177 4 12.833886341779198 17.74845752602092 37.73080499026297 31.686851141936913 5 12.597976426119331 36.829822951421654 35.14836204536693 15.423838577092083 6 30.126710904887826 37.97285835453774 17.540338272713665 14.360092467860767 7 26.1348026318853 32.74064248495101 22.521880822773486 18.602674060390207 8 24.70939766121162 35.292501548304784 20.864113721073643 19.133987069409955 9 24.380237171766762 16.762425441034182 22.264480479545572 36.592856907653484 10-11 23.50640974896508 26.757201197133956 28.71499184770136 21.021397206199605 12-13 24.709121008903256 24.321196429517293 28.989047026167945 21.980635535411505 14-15 21.986918232759862 26.5004281122706 26.986880398228813 24.52577325674072 16-17 20.595164366439008 29.61897899028576 27.59795064982235 22.187905993452883 18-19 20.343103341610917 27.571383336315748 30.951967960235677 21.133545361837662 20-21 21.659528277599904 27.007549274674925 30.657607115749336 20.675315331975835 22-23 21.261933255591025 27.060683901688133 30.726341204464706 20.951041638256132 24-25 21.096758571554965 26.934008955221895 30.829567066709153 21.139665406513988 26-27 20.890098965113747 26.770987928211877 30.937100243005688 21.401812863668688 28-29 20.947650974789607 26.841524024010916 31.015910403024904 21.194914598174577 30-31 21.427877694491677 27.355360245654087 30.41856089331662 20.798201166537613 32-33 20.824283539008963 27.11136552166396 30.924968252268243 21.139382687058834 34-35 20.898771800164575 26.61437798061143 30.916420146388806 21.570430072835183 36-37 21.60527108804416 27.142780642298685 30.154083766115843 21.09786450354131 38-39 21.41041917649477 27.104347426953606 30.36700477696096 21.118228619590663 40-41 21.1017061620202 26.88657029370227 30.5647005136181 21.44702303065943 42-43 21.11956737936361 26.919399011612782 30.329428035259443 21.63160557376417 44-45 21.408057637516407 27.155636062230208 30.031649611476947 21.404656688776434 46-47 21.336413201468545 27.537165966963734 29.5300387758048 21.59638205576292 48-49 21.289090953842223 27.14703806468222 29.631576636596403 21.932294344879153 50-51 21.349285251956253 27.107490602072698 29.41280166997393 22.13042247599712 52-53 21.281939814682385 27.266038009468772 29.304362128365273 22.147660047483562 54-55 21.414111159967987 27.69615408409873 29.055760259223806 21.83397449670948 56-57 21.020931550626408 27.384771865356356 29.111381154386773 22.482915429630463 58-59 20.905356891319688 26.904454516290567 29.49100793095517 22.699180661434575 60-61 21.3282226525471 27.313892434893035 29.004280372551065 22.353604540008796 62-63 21.062408157753467 27.294151964700646 29.121376118654364 22.522063758891527 64-65 20.948031507586524 26.976907474902827 29.38434678834025 22.690714229170396 66-67 20.927933480436145 27.049824148498892 29.028660767919256 22.993581603145703 68-69 21.43267917594929 27.27128494994535 28.73370953868833 22.562326335417033 70-71 21.367129838743477 26.90337547072789 28.818841355802764 22.910653334725865 72-73 21.00997201410005 27.045733031677216 29.038156815501274 22.906138138721456 74-75 21.0422935000469 26.95971979508494 28.848443745813256 23.149542959054905 76-77 21.065509756482093 27.0812642149679 28.53953116466446 23.31369486388555 78-79 21.20218193006016 27.11684218841598 28.558720444368685 23.122255437155175 80-81 20.940300644618596 26.90481460791786 28.681536194915925 23.473348552547616 82-83 20.75802270492536 26.8612529909093 28.779584276600122 23.60114002756522 84-85 20.837654506182243 26.742774189642887 28.552336692927422 23.867234611247447 86-87 20.975141058310477 26.832697838152246 28.475031920276432 23.717129183260845 88-89 20.731929497376903 26.663570055302454 28.531224178447967 24.07327626887268 90-91 20.587685751925424 26.48398439621046 28.483509926324125 24.444819925539992 92-93 20.717503212419842 26.647081847210636 28.281892346246014 24.353522594123504 94-95 20.583783165302563 26.353046556236787 28.481606310076224 24.581563968384426 96-97 20.4800178013503 26.286328000855473 28.562851954597907 24.670802243196313 98-99 20.47403250260081 26.29177669514678 28.434690916462785 24.79949988578963 100 20.648869827895595 26.264955567773573 28.29945494561929 24.78671965871154 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1291.0 1 1001.5 2 2275.0 3 3558.0 4 2098.5 5 964.5 6 1795.5 7 4499.5 8 7281.0 9 8133.5 10 6399.0 11 4114.0 12 3579.0 13 3959.5 14 4543.5 15 5039.0 16 5718.0 17 6518.5 18 7399.0 19 8289.5 20 9408.0 21 11188.5 22 13853.5 23 17968.5 24 23335.5 25 29946.5 26 39179.5 27 51425.5 28 67156.5 29 84359.5 30 99999.5 31 114911.5 32 129336.5 33 142814.0 34 156142.0 35 168008.0 36 177535.0 37 186360.0 38 194472.0 39 199466.0 40 202029.0 41 204428.5 42 204491.0 43 204159.0 44 203542.5 45 201985.5 46 200181.5 47 196915.5 48 193605.0 49 191493.0 50 187756.5 51 180825.0 52 172520.0 53 166438.0 54 162715.0 55 156549.0 56 146896.5 57 134239.0 58 121386.0 59 109056.0 60 94432.5 61 78359.5 62 64495.5 63 52375.5 64 41505.0 65 32898.5 66 25936.5 67 19996.5 68 14977.0 69 11087.5 70 8190.0 71 6129.5 72 4729.5 73 3629.5 74 2604.5 75 1794.5 76 1251.5 77 892.0 78 661.5 79 543.5 80 354.0 81 136.5 82 69.0 83 35.0 84 15.5 85 8.5 86 7.0 87 6.5 88 5.5 89 3.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061965452347802145 2 1.995666742280262E-4 3 0.0 4 0.11794390446876349 5 0.0016963167309382227 6 0.002128711191765613 7 0.0 8 0.0 9 3.3261112371337704E-5 10-11 0.0 12-13 5.820694664984097E-5 14-15 4.157639046417213E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.0788195232086066E-4 30-31 3.32611123713377E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.1641389329968195E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.989166855700655E-5 80-81 2.4945834278503276E-4 82-83 2.5777362087786717E-4 84-85 0.0 86-87 8.315278092834426E-6 88-89 5.820694664984097E-5 90-91 2.4945834278503275E-5 92-93 6.236458569625819E-4 94-95 6.984833597980918E-4 96-97 1.6630556185668852E-5 98-99 2.4945834278503275E-5 100 8.315278092834427E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6013028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.13349702526405 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04196143316283 49.27061939986357 2 13.52276014781493 17.07478275130866 3 3.8680984019850353 7.326197368654526 4 1.6151852722580609 4.078891783254183 5 0.8443785966907622 2.6654286811186436 6 0.48900312418695085 1.8523486371721014 7 0.3389301020034194 1.4978489810623725 8 0.22292724139109962 1.1259341064972248 9 0.16891338624588673 0.9597683491273771 >10 0.8326362592701503 9.118312548393813 >50 0.0383793097030263 1.6596575697461817 >100 0.015720327903321982 1.7204902257897743 >500 6.32235228901697E-4 0.2599300519095076 >1k 3.6879278776948116E-4 0.46903962793204024 >5k 2.6342341946432523E-5 0.09635909663097318 >10k+ 7.902702583929757E-5 0.8243908215390646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19365 0.32205072053547734 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18904 0.314384034133884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11301 0.18794191545424369 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 1.6630556185668852E-5 4 0.0 0.0 0.0 1.6630556185668852E-5 1.6630556185668852E-5 5 0.0 0.0 0.0 1.6630556185668852E-5 1.6630556185668852E-5 6 0.0 0.0 0.0 1.6630556185668852E-5 1.6630556185668852E-5 7 0.0 0.0 0.0 1.6630556185668852E-5 1.6630556185668852E-5 8 0.0 0.0 0.0 1.6630556185668852E-5 1.6630556185668852E-5 9 0.0 0.0 0.0 4.989166855700655E-5 1.6630556185668852E-5 10-11 0.0 0.0 0.0 4.989166855700655E-5 1.6630556185668852E-5 12-13 0.0 0.0 0.0 4.989166855700655E-5 1.6630556185668852E-5 14-15 0.0 8.315278092834426E-6 0.0 7.483750283550983E-5 1.6630556185668852E-5 16-17 0.0 1.6630556185668852E-5 1.6630556185668852E-5 9.97833371140131E-5 1.6630556185668852E-5 18-19 0.0 1.6630556185668852E-5 1.6630556185668852E-5 1.2472917139251638E-4 1.6630556185668852E-5 20-21 0.0 1.6630556185668852E-5 1.6630556185668852E-5 1.8293611804235738E-4 1.6630556185668852E-5 22-23 0.0 1.6630556185668852E-5 1.6630556185668852E-5 5.654389103127409E-4 1.6630556185668852E-5 24-25 0.0 1.6630556185668852E-5 1.6630556185668852E-5 0.0016713708966597195 1.6630556185668852E-5 26-27 0.0 1.6630556185668852E-5 1.6630556185668852E-5 0.0038998654255393453 1.6630556185668852E-5 28-29 0.0 4.989166855700655E-5 1.6630556185668852E-5 0.011192364312955137 1.6630556185668852E-5 30-31 0.0 4.989166855700655E-5 1.6630556185668852E-5 0.03166457897751349 1.6630556185668852E-5 32-33 0.0 4.989166855700655E-5 1.6630556185668852E-5 0.08174749893065525 1.6630556185668852E-5 34-35 0.0 4.989166855700655E-5 1.6630556185668852E-5 0.1614494394504732 1.6630556185668852E-5 36-37 0.0 6.652222474267541E-5 1.6630556185668852E-5 0.2882241692538269 1.6630556185668852E-5 38-39 0.0 9.146805902117868E-5 1.6630556185668852E-5 0.46260386613865756 1.6630556185668852E-5 40-41 0.0 9.97833371140131E-5 1.6630556185668852E-5 0.663168373737824 1.6630556185668852E-5 42-43 0.0 9.97833371140131E-5 1.6630556185668852E-5 0.8704932024264647 1.6630556185668852E-5 44-45 0.0 9.97833371140131E-5 1.6630556185668852E-5 1.1237932036903868 1.6630556185668852E-5 46-47 0.0 9.97833371140131E-5 1.6630556185668852E-5 1.433512366814191 1.6630556185668852E-5 48-49 0.0 1.0809861520684754E-4 1.6630556185668852E-5 1.7635374390406964 1.6630556185668852E-5 50-51 0.0 1.3304444948535082E-4 1.6630556185668852E-5 2.105353575602841 1.6630556185668852E-5 52-53 0.0 1.4967500567101966E-4 1.6630556185668852E-5 2.440110041064169 1.6630556185668852E-5 54-55 0.0 1.4967500567101966E-4 2.494583427850328E-5 2.8202097179657235 1.6630556185668852E-5 56-57 0.0 1.4967500567101966E-4 3.3261112371337704E-5 3.265201825103758 1.6630556185668852E-5 58-59 0.0 1.4967500567101966E-4 4.989166855700655E-5 3.742390689017247 1.6630556185668852E-5 60-61 0.0 1.579902837638541E-4 4.989166855700655E-5 4.2216666877320375 1.6630556185668852E-5 62-63 0.0 1.663055618566885E-4 4.989166855700655E-5 4.6919705013846595 2.494583427850328E-5 64-65 0.0 1.663055618566885E-4 4.989166855700655E-5 5.20765078758988 3.3261112371337704E-5 66-67 1.6630556185668852E-5 1.663055618566885E-4 4.989166855700655E-5 5.79822678357726 3.3261112371337704E-5 68-69 1.6630556185668852E-5 1.663055618566885E-4 4.989166855700655E-5 6.42016800853081 3.3261112371337704E-5 70-71 1.6630556185668852E-5 1.663055618566885E-4 4.989166855700655E-5 7.070281395662883 3.3261112371337704E-5 72-73 1.6630556185668852E-5 1.995666742280262E-4 6.652222474267541E-5 7.7546953049279 3.3261112371337704E-5 74-75 1.6630556185668852E-5 2.0788195232086064E-4 6.652222474267541E-5 8.424441063637156 4.989166855700655E-5 76-77 1.6630556185668852E-5 2.1619723041369507E-4 6.652222474267541E-5 9.120313426114098 4.989166855700655E-5 78-79 1.6630556185668852E-5 2.1619723041369507E-4 6.652222474267541E-5 9.878101349270285 4.989166855700655E-5 80-81 1.6630556185668852E-5 2.3282778659936392E-4 6.652222474267541E-5 10.669075547294973 4.989166855700655E-5 82-83 1.6630556185668852E-5 2.3282778659936392E-4 6.652222474267541E-5 11.461346928702145 4.989166855700655E-5 84-85 1.6630556185668852E-5 2.3282778659936392E-4 6.652222474267541E-5 12.224506521506303 4.989166855700655E-5 86-87 1.6630556185668852E-5 2.3282778659936392E-4 6.652222474267541E-5 13.016495183458318 4.989166855700655E-5 88 1.6630556185668852E-5 2.6608889897070164E-4 6.652222474267541E-5 13.642477633564987 4.989166855700655E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11580 0.0 56.029716 1 GTATCAA 20585 0.0 39.158916 1 GTGGTAT 4780 0.0 36.150635 1 TGGTATC 5200 0.0 31.853462 2 ATCAACG 25850 0.0 30.745846 3 TCAACGC 26205 0.0 30.419115 4 TATCAAC 26455 0.0 30.113869 2 CAACGCA 27235 0.0 29.371216 5 AACGCAG 28560 0.0 28.123308 6 ACGCAGA 31910 0.0 25.066914 7 CGCAGAG 32160 0.0 24.82821 8 GTACATG 14530 0.0 22.910341 1 GCAGAGT 34570 0.0 22.811857 9 TATAACG 625 0.0 21.8341 2 ATAACGC 630 0.0 21.660816 3 GAGTACT 26400 0.0 21.166351 12-13 TACATGG 15020 0.0 20.959143 2 AGAGTAC 32445 0.0 20.51809 10-11 CAGAGTA 34245 0.0 20.407127 10-11 ACATGGG 15070 0.0 19.796724 3 >>END_MODULE