FastQCFastQC Report
Wed 25 May 2016
SRR2049488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6763947
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.043.0674821
GTATCAA97900.034.9633371
ATCAACG101400.032.53883
TCAACGC102450.031.9552694
CAACGCA110350.029.8530435
TATCAAC118700.028.0553862
AACGCAG120600.027.4852496
GTACATG161900.023.3259721
ACGCAGA142100.023.0378657
CGCAGAG145800.022.2354078
TACATGG167500.021.4109952
ACATGGG172400.019.821763
GTGGTAT39000.018.9198191
GCAGAGT169300.018.6682519
TAGACCG3902.0263542E-917.0776755
GAGTACA130550.016.838351
AGAGTAC145100.016.8115410-11
CAGAGTA161450.016.2572710-11
CATGGGG124750.015.8525954
GTGTTAG30550.014.7601431
Produced by FastQC (version 0.11.5)