##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6763947 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89640397832804 31.0 31.0 34.0 28.0 34.0 2 31.049881230589182 31.0 31.0 34.0 28.0 34.0 3 31.11202039282685 31.0 31.0 34.0 28.0 34.0 4 34.704715308975665 37.0 35.0 37.0 32.0 37.0 5 34.711180173351444 37.0 35.0 37.0 32.0 37.0 6 34.737245280011805 37.0 35.0 37.0 32.0 37.0 7 30.701753724563485 37.0 33.0 37.0 2.0 37.0 8 32.49024437950209 37.0 33.0 37.0 17.0 37.0 9 35.28318155065378 39.0 34.0 39.0 28.0 39.0 10-11 35.85508490826436 39.0 36.0 39.0 30.5 39.0 12-13 36.043435585760804 39.0 37.0 39.0 31.0 39.0 14-15 37.2598501289262 40.0 37.5 41.0 31.0 41.0 16-17 37.27542505877116 40.0 38.0 41.0 31.0 41.0 18-19 37.278691790459035 40.0 37.5 41.0 31.0 41.0 20-21 37.3343840512056 40.0 38.0 41.0 31.0 41.0 22-23 37.29435904805286 40.0 37.5 41.0 31.0 41.0 24-25 37.278112247183486 40.0 37.0 41.0 31.0 41.0 26-27 37.29927932610944 40.0 37.0 41.0 31.0 41.0 28-29 37.28054721599681 40.0 37.0 41.0 31.0 41.0 30-31 37.20090008097343 40.0 37.0 41.0 31.0 41.0 32-33 37.157373719811815 40.0 37.0 41.0 31.0 41.0 34-35 37.0930711757499 40.0 37.0 41.0 31.0 41.0 36-37 36.93472649918753 40.0 37.0 41.0 30.0 41.0 38-39 36.815897877378404 40.0 37.0 41.0 30.0 41.0 40-41 36.72855856203486 40.0 37.0 41.0 30.0 41.0 42-43 36.60298920142337 40.0 36.0 41.0 30.0 41.0 44-45 36.47666421691358 40.0 36.0 41.0 30.0 41.0 46-47 36.32277226595655 40.0 36.0 41.0 30.0 41.0 48-49 36.237926243360576 40.0 36.0 41.0 29.5 41.0 50-51 36.10853973279211 39.0 35.0 41.0 29.0 41.0 52-53 35.975656225573616 39.0 35.0 41.0 29.0 41.0 54-55 35.84696516693582 39.0 35.0 41.0 29.0 41.0 56-57 35.642441831670176 39.0 35.0 41.0 28.0 41.0 58-59 35.45680192349231 39.0 35.0 40.5 28.0 41.0 60-61 35.232797063607975 38.5 34.5 40.0 28.0 41.0 62-63 34.97814863126515 38.0 34.0 40.0 27.5 41.0 64-65 34.62849058397412 37.5 34.0 40.0 26.5 41.0 66-67 31.13159003167825 36.0 30.5 40.0 6.5 41.0 68-69 31.48597453528243 36.0 30.5 39.0 11.0 41.0 70-71 31.64620250572632 35.0 30.0 39.0 15.5 41.0 72-73 32.877184504846056 35.0 32.0 39.0 24.0 40.5 74-75 32.958946529297165 35.0 33.0 38.5 25.0 40.0 76-77 32.65622010343961 35.0 33.0 37.0 24.5 39.5 78-79 29.278982301310165 35.0 29.0 36.5 6.5 39.0 80-81 31.076296354776286 35.0 30.0 36.0 19.5 39.0 82-83 31.487101983501645 35.0 32.0 36.0 22.0 37.5 84-85 29.662200265614146 35.0 30.5 35.5 11.0 37.0 86-87 29.29713878597807 34.0 28.5 35.0 12.5 37.0 88-89 30.531104767674847 34.0 30.5 35.0 19.0 36.0 90-91 30.69166967156898 35.0 31.5 35.0 18.5 36.0 92-93 30.546744674374295 35.0 32.0 35.0 16.0 36.0 94-95 30.33758040978145 35.0 32.0 35.0 8.5 36.0 96-97 30.149329378246165 35.0 32.0 35.0 2.0 35.0 98-99 29.955708109481044 35.0 31.5 35.0 2.0 35.0 100 29.755246751637763 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.12632710954700954 1101 2 0.1495616141483076 1101 3 0.19100725360858206 1101 4 0.13424661820206296 1101 5 0.1642587723211264 1101 6 0.1867823054166564 1101 7 -12.184979826439893 1101 8 -6.68809052837355 1101 9 -2.453113256679032 1101 10-11 -0.3475442518552967 1101 12-13 0.17728527310584496 1101 14-15 0.34636049915098255 1101 16-17 0.37160987928657363 1101 18-19 0.42798312089440316 1101 20-21 0.3823520749878071 1101 22-23 0.31012393692691376 1101 24-25 0.4018070860770493 1101 26-27 0.3373944539046647 1101 28-29 0.28479724852764576 1101 30-31 0.3587316154438156 1101 32-33 0.3381862149483936 1101 34-35 0.3355130723170632 1101 36-37 0.4145857027845068 1101 38-39 0.38545629802595727 1101 40-41 0.36049810672270155 1101 42-43 0.28735114022945396 1101 44-45 0.220221252372248 1101 46-47 0.25619742916194355 1101 48-49 0.19632443470715089 1101 50-51 0.2805236598704539 1101 52-53 0.3053966919252389 1101 54-55 0.23002162337568421 1101 56-57 0.2165064505587253 1101 58-59 0.14261828363663653 1101 60-61 0.14612347384140634 1101 62-63 0.1328548388868782 1101 64-65 0.023846980286442943 1101 66-67 -10.371585573544976 1101 68-69 -8.315898727240771 1101 70-71 -6.871040794369389 1101 72-73 -1.7857942732309091 1101 74-75 -0.5388627742673364 1101 76-77 -0.25547594151123576 1101 78-79 2.877247983056776 1101 80-81 0.7547090133832839 1101 82-83 0.0867610797512306 1101 84-85 -5.542168964953941 1101 86-87 -6.083570365528896 1101 88-89 -1.6273333673999808 1101 90-91 -0.35464669827078055 1101 92-93 0.009018134615587314 1101 94-95 0.0246702945126529 1101 96-97 0.048789943958606585 1101 98-99 0.12507427883050326 1101 100 0.1681741480431107 1102 1 -0.022782510585905413 1102 2 0.02181385366570865 1102 3 0.015967802005842913 1102 4 -0.018700419677784907 1102 5 -0.07908302559495439 1102 6 -0.03361666587570511 1102 7 -12.353506513645371 1102 8 -6.839629908666808 1102 9 -2.6223951722999317 1102 10-11 -0.6735784707303836 1102 12-13 -0.05025057831464608 1102 14-15 0.04986444515267152 1102 16-17 0.03596410987409371 1102 18-19 0.036766755725899714 1102 20-21 0.011703531026455494 1102 22-23 0.04589220509095071 1102 24-25 0.03034423621358684 1102 26-27 0.03062757322540932 1102 28-29 0.00815763529008251 1102 30-31 -0.009397684423284147 1102 32-33 0.027615194280421917 1102 34-35 0.038945225026211006 1102 36-37 0.15081706187592658 1102 38-39 0.11904693448096282 1102 40-41 -0.002885662818513879 1102 42-43 -0.0958406995257306 1102 44-45 -0.035837382112170246 1102 46-47 -0.038595905862621294 1102 48-49 -0.11774224407582068 1102 50-51 -0.055347502196440246 1102 52-53 -0.08942893220100956 1102 54-55 -0.12115604535537017 1102 56-57 -0.07479198361967576 1102 58-59 -0.15456712690614438 1102 60-61 -0.2492842796659076 1102 62-63 -0.23395935847336347 1102 64-65 -0.3408353475411232 1102 66-67 -10.641673296828849 1102 68-69 -7.121851102183079 1102 70-71 -6.703031574200626 1102 72-73 -1.6932882161319966 1102 74-75 -0.7888731446433184 1102 76-77 -0.6089511744029537 1102 78-79 2.5313117610794045 1102 80-81 0.42658482718434243 1102 82-83 -0.2634466958851611 1102 84-85 -5.930803390479287 1102 86-87 -6.375407659588586 1102 88-89 -1.9686848629848903 1102 90-91 -0.7367022771754606 1102 92-93 -0.4086233684284224 1102 94-95 -0.33828838427200125 1102 96-97 -0.23069392894980822 1102 98-99 -0.2579467652496952 1102 100 -0.19475480793979472 1103 1 0.07352901752923202 1103 2 0.1074400747783315 1103 3 0.08710227037696328 1103 4 0.11346305109817933 1103 5 0.05742557356525424 1103 6 0.09989660727401173 1103 7 -12.274423715447245 1103 8 -6.7412045647607926 1103 9 -2.4269740559848927 1103 10-11 -0.5366488024528095 1103 12-13 0.024073766858400347 1103 14-15 0.21098555404802255 1103 16-17 0.17275315449496986 1103 18-19 0.23746318513651943 1103 20-21 0.2520573947336118 1103 22-23 0.27437573508628077 1103 24-25 0.3274153218805935 1103 26-27 0.2691620330502005 1103 28-29 0.24752704712499707 1103 30-31 0.24393810927847426 1103 32-33 0.29525952752108253 1103 34-35 0.27968319649912843 1103 36-37 0.329379041489986 1103 38-39 0.337326370622165 1103 40-41 0.3231949783957191 1103 42-43 0.3283099379522767 1103 44-45 0.24733622012400502 1103 46-47 0.26819428469717366 1103 48-49 0.23603635504277776 1103 50-51 0.21056850849885933 1103 52-53 0.12597834352661152 1103 54-55 0.17884753760689875 1103 56-57 0.21622350502887855 1103 58-59 0.1931644976485316 1103 60-61 0.18443798269970557 1103 62-63 0.09377289639847675 1103 64-65 -0.05523104035356852 1103 66-67 -10.485248735896512 1103 68-69 -8.439990026317965 1103 70-71 -6.965857381435427 1103 72-73 -1.8325250837378846 1103 74-75 -0.5941308068284386 1103 76-77 -0.2885901640563091 1103 78-79 2.829798918697165 1103 80-81 0.7274070967260258 1103 82-83 0.05580821442253381 1103 84-85 -5.714298134740535 1103 86-87 -6.197646172616611 1103 88-89 -1.6476709313516693 1103 90-91 -0.35802142035418427 1103 92-93 0.03759173943142713 1103 94-95 0.05715160095767047 1103 96-97 0.18210346326265991 1103 98-99 0.1969569702980536 1103 100 0.25686826889505454 1104 1 -0.057509145731831524 1104 2 -0.05206521404666731 1104 3 -0.04098745248749225 1104 4 0.009185960226815837 1104 5 -0.010992303234353074 1104 6 -0.01300771121165667 1104 7 -12.388305801855928 1104 8 -6.857174719833406 1104 9 -2.7101514720278956 1104 10-11 -0.6153196728455725 1104 12-13 -0.04052027276847525 1104 14-15 0.15296972295974598 1104 16-17 0.16848063760195942 1104 18-19 0.11828488492041345 1104 20-21 0.1240629258902004 1104 22-23 0.1134830676121581 1104 24-25 0.17137589383786178 1104 26-27 0.16561427535042128 1104 28-29 0.13264901913725424 1104 30-31 0.0701347135390904 1104 32-33 0.03960317841316652 1104 34-35 0.0025821079097525512 1104 36-37 0.06603624983438294 1104 38-39 0.08184544629507684 1104 40-41 0.13575499973293148 1104 42-43 0.08284330454220878 1104 44-45 0.0837435227472767 1104 46-47 0.04172351729946655 1104 48-49 0.039265111227472005 1104 50-51 0.015180087789957497 1104 52-53 0.03978252675874927 1104 54-55 0.009371165174208329 1104 56-57 -0.021755703377117186 1104 58-59 -0.0621594714942546 1104 60-61 -0.07509379910472092 1104 62-63 -0.1481778234772193 1104 64-65 -0.2688704109392219 1104 66-67 -10.638773819704433 1104 68-69 -8.583790054933957 1104 70-71 -7.111556935523964 1104 72-73 -2.107958552602902 1104 74-75 -0.8415509772734637 1104 76-77 -0.5449802999973983 1104 78-79 2.633124879199144 1104 80-81 0.5458497597532777 1104 82-83 -0.11134747528149092 1104 84-85 -5.814523213481561 1104 86-87 -6.328168336976596 1104 88-89 -1.8035298648136369 1104 90-91 -0.5223174372496118 1104 92-93 -0.2010567116753137 1104 94-95 -0.15569402816549527 1104 96-97 -0.029540817334474667 1104 98-99 -9.408800931893779E-4 1104 100 0.10343981207792297 1105 1 -0.04266416306613152 1105 2 -0.026871134267096863 1105 3 -0.05385506558486952 1105 4 -0.0344473541725705 1105 5 -0.03865069749153349 1105 6 0.008605491860478764 1105 7 -12.264869647932766 1105 8 -6.79599664977577 1105 9 -2.6925692152415763 1105 10-11 -0.628622060852031 1105 12-13 -0.043428487585323694 1105 14-15 0.08472444155903958 1105 16-17 0.08723185171848513 1105 18-19 0.06182187419311447 1105 20-21 0.12170008395439424 1105 22-23 0.05269968875472841 1105 24-25 0.09468637648382838 1105 26-27 0.059372729566440796 1105 28-29 0.00873104810538905 1105 30-31 0.05996751791192878 1105 32-33 0.05105625643196987 1105 34-35 0.08058171759912369 1105 36-37 0.15620279930263337 1105 38-39 0.10083050212823252 1105 40-41 0.08104752749259347 1105 42-43 0.012721864587810217 1105 44-45 -0.07508640600357097 1105 46-47 -0.0752327125607195 1105 48-49 -0.1056851129601597 1105 50-51 -0.0803785214481536 1105 52-53 -0.057341957726833925 1105 54-55 -0.09023233068187864 1105 56-57 -0.10934518279663763 1105 58-59 -0.10521629127940457 1105 60-61 -0.23816399394974752 1105 62-63 -0.2948212212621115 1105 64-65 -0.4134026726786715 1105 66-67 -10.5329630792207 1105 68-69 -8.597129537767337 1105 70-71 -7.064626496726017 1105 72-73 -2.1308162980933645 1105 74-75 -1.0075047987112669 1105 76-77 -0.6716900548842304 1105 78-79 2.4490012007963387 1105 80-81 0.326700985109877 1105 82-83 -0.3148527407481012 1105 84-85 -5.849464957277103 1105 86-87 -6.335485797130083 1105 88-89 -1.9993660515173595 1105 90-91 -0.7228848139058819 1105 92-93 -0.3892445409742109 1105 94-95 -0.32735388675065735 1105 96-97 -0.23226817490523644 1105 98-99 -0.2486071003641115 1105 100 -0.19464525983354974 1106 1 -0.06313349410862656 1106 2 -0.036147895214199366 1106 3 -0.013049494925780891 1106 4 -0.042511031956607326 1106 5 -0.09333720725486927 1106 6 -0.04918055646739106 1106 7 -12.40511654939829 1106 8 -6.886097446471737 1106 9 -2.769000492177142 1106 10-11 -0.6663467590349015 1106 12-13 -0.1010733168011555 1106 14-15 0.006100050764622722 1106 16-17 0.005702502874513016 1106 18-19 0.06061982243043218 1106 20-21 0.05184980398388461 1106 22-23 0.040387185021060645 1106 24-25 0.09359939683620411 1106 26-27 0.057491642870289184 1106 28-29 0.04295871999921275 1106 30-31 -0.006119833338743774 1106 32-33 -0.010975243747665786 1106 34-35 0.07504554215008596 1106 36-37 0.09394694875263099 1106 38-39 0.07846711265678863 1106 40-41 0.056275950299585986 1106 42-43 0.034322174237722436 1106 44-45 -0.04669064968876313 1106 46-47 0.009404551294487362 1106 48-49 -0.02951525171934577 1106 50-51 -0.009927385205770634 1106 52-53 -0.025717538021908126 1106 54-55 -0.06962202928792038 1106 56-57 -0.11411993381263841 1106 58-59 -0.16382574160466845 1106 60-61 -0.13647025833421367 1106 62-63 -0.23020856335478612 1106 64-65 -0.32129877901315496 1106 66-67 -10.698138087073392 1106 68-69 -8.678857958385237 1106 70-71 -7.162301098330094 1106 72-73 -2.192558005782004 1106 74-75 -0.9607360483066927 1106 76-77 -0.6938114817372067 1106 78-79 2.490345953198524 1106 80-81 0.3885962549575588 1106 82-83 -0.306632977016001 1106 84-85 -5.957164356419927 1106 86-87 -6.411362084719297 1106 88-89 -1.951695444555689 1106 90-91 -0.7434107402386658 1106 92-93 -0.36092805786751825 1106 94-95 -0.25350525106034283 1106 96-97 -0.15281320424034917 1106 98-99 -0.1680044524734221 1106 100 -0.07058966801624322 1107 1 -0.08461545199685361 1107 2 -0.056210853705703556 1107 3 -0.05960570034289603 1107 4 -0.0585905145327672 1107 5 -0.022423772599879044 1107 6 -0.06466188737609002 1107 7 -12.315364514954023 1107 8 -6.841381588879376 1107 9 -2.7022452035560605 1107 10-11 -0.7292344318049615 1107 12-13 -0.060177602147902576 1107 14-15 0.05435712160378614 1107 16-17 0.03399216235784763 1107 18-19 0.06928281686371207 1107 20-21 0.09187465229616976 1107 22-23 0.16439805775752347 1107 24-25 0.19225264176598245 1107 26-27 0.04388159003876524 1107 28-29 0.03874457734823267 1107 30-31 0.08755109525071703 1107 32-33 0.08464487011077892 1107 34-35 0.05166199480065359 1107 36-37 0.13083045180688657 1107 38-39 0.09904349929331602 1107 40-41 0.12494755393841217 1107 42-43 0.09840848536388336 1107 44-45 0.06695219790082518 1107 46-47 0.10285808590032985 1107 48-49 0.09487918335985057 1107 50-51 0.060364029651680084 1107 52-53 0.08371175945026721 1107 54-55 0.012296860573485446 1107 56-57 0.051203924496306286 1107 58-59 -0.0019913003301184062 1107 60-61 -0.023573090103944594 1107 62-63 -0.059516438671955996 1107 64-65 -0.25704618102118815 1107 66-67 -10.500399872864588 1107 68-69 -8.521796383806365 1107 70-71 -7.0228146093420385 1107 72-73 -1.9994870468407733 1107 74-75 -0.7218845630629147 1107 76-77 -0.4580262479968482 1107 78-79 2.6932802610819166 1107 80-81 0.5893678591968197 1107 82-83 -0.13820304455850518 1107 84-85 -5.7863523468336275 1107 86-87 -6.242784062760336 1107 88-89 -1.7746870261196115 1107 90-91 -0.5605981175074071 1107 92-93 -0.17249187896500473 1107 94-95 -0.09922869838449344 1107 96-97 -0.04511323574733339 1107 98-99 0.0449700295372395 1107 100 0.12248134798377919 1108 1 -0.15370616000802073 1108 2 -0.15188722389406806 1108 3 -0.09910091521775044 1108 4 -0.22139099212853353 1108 5 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-0.152737936481131 2215 58-59 -0.24207553566033368 2215 60-61 -0.23049076565268933 2215 62-63 -0.3099850201339862 2215 64-65 -0.2056073103816516 2215 66-67 3.120247737231253 2215 68-69 2.4790886147875426 2215 70-71 1.9968679334487582 2215 72-73 0.3729352111730364 2215 74-75 0.03624891126096941 2215 76-77 -0.0808855386598637 2215 78-79 2.979588946633271 2215 80-81 0.7933850021689679 2215 82-83 0.077444405073539 2215 84-85 1.7226725718329874 2215 86-87 1.933478175323323 2215 88-89 0.5344520666126833 2215 90-91 0.13024543791324916 2215 92-93 -0.1727738056836401 2215 94-95 -0.10101789683250928 2215 96-97 -0.17974719608612233 2215 98-99 -0.24759935473328198 2215 100 -0.20289406929006404 2216 1 -0.15535342808202657 2216 2 -0.0043292715366121115 2216 3 -0.06513535080010868 2216 4 -0.04300678103492572 2216 5 0.07679167376569751 2216 6 -0.10068384491624016 2216 7 4.043253584265901 2216 8 2.1151292598479827 2216 9 0.8506664053181225 2216 10-11 0.249079719135473 2216 12-13 -0.06747302778450859 2216 14-15 -0.14255116481246688 2216 16-17 -0.311161729203036 2216 18-19 -0.6257277949684052 2216 20-21 -0.24291492945889104 2216 22-23 -0.13798763563164584 2216 24-25 -0.1855387855122146 2216 26-27 -0.47142848459381526 2216 28-29 -0.510555860158938 2216 30-31 -0.4015921105487905 2216 32-33 -1.2309988478614997 2216 34-35 -0.3865989751038299 2216 36-37 -0.20612849561819502 2216 38-39 -0.3210823207311222 2216 40-41 -0.2656727368799139 2216 42-43 -0.0022854774213030282 2216 44-45 -0.12857649532694637 2216 46-47 -0.20215323348600833 2216 48-49 -0.29272791356128636 2216 50-51 -0.622955148012899 2216 52-53 -0.4161804781806282 2216 54-55 -0.18231524948775757 2216 56-57 -0.1257491483668005 2216 58-59 -0.30907895491399984 2216 60-61 -0.46797576919535544 2216 62-63 -0.2892830170861842 2216 64-65 -0.07810888830981355 2216 66-67 3.2505444337741736 2216 68-69 2.5075024756687156 2216 70-71 2.137569301027437 2216 72-73 0.38558458223256054 2216 74-75 -0.3712666930364037 2216 76-77 -0.08708534836704729 2216 78-79 2.625555288463673 2216 80-81 1.04133267737528 2216 82-83 0.16166589604578618 2216 84-85 1.8888327767712703 2216 86-87 1.8767879236448053 2216 88-89 0.6372034944227778 2216 90-91 0.325915856815314 2216 92-93 0.33274929080492655 2216 94-95 0.3438926759820191 2216 96-97 0.29997468230739344 2216 98-99 0.319359914254143 2216 100 -0.5659854188463846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97779.0 3 18219.0 4 10560.0 5 9553.0 6 9135.0 7 9933.0 8 11201.0 9 12725.0 10 14434.0 11 16030.0 12 18258.0 13 20749.0 14 24340.0 15 26321.0 16 27596.0 17 28135.0 18 26255.0 19 25171.0 20 24141.0 21 24709.0 22 26077.0 23 30254.0 24 35631.0 25 44901.0 26 56816.0 27 69149.0 28 85994.0 29 109313.0 30 135678.0 31 168198.0 32 226171.0 33 321897.0 34 491508.0 35 666599.0 36 760227.0 37 1257452.0 38 1376776.0 39 445119.0 40 943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.06524487529629 20.411026153252408 14.318813277873316 23.204915693577984 2 15.673136246159524 20.24300213089991 40.167677709409006 23.91618391353156 3 18.382637649975006 24.964898090252085 30.20872496646524 26.443739293307672 4 12.843795804327316 17.04773304339881 38.31812054301811 31.790350609255768 5 13.243899994174912 37.49009069692006 35.16902957281996 14.09697973608506 6 31.00889139440939 37.56889503736917 17.724270820350483 13.697942747870961 7 27.16202461925652 32.42348444695791 22.333673275891528 18.08081765789405 8 24.727958394706523 36.3066268851604 20.66068820468286 18.304726515450227 9 24.489591654103734 17.495657491106893 21.830641192191482 36.18410966259789 10-11 23.74467895741939 27.210222078913393 28.73460569693997 20.310493266727253 12-13 24.114633068532324 24.676886143549027 28.773577025366993 22.43490376255166 14-15 21.651588931728767 27.090558219926912 25.918247141794577 25.33960570654974 16-17 21.2875894412926 30.219011598871308 26.62185940903622 21.871539550799877 18-19 21.14223396487288 29.201648090974103 28.530656730456343 21.125461213696678 20-21 22.267213211457747 28.546438935727913 28.036817852061823 21.14953000075252 22-23 21.95445366103945 28.444178261906718 28.04614140576068 21.555226671293156 24-25 21.81557602388073 28.454044657653288 28.071435213788636 21.658944104677342 26-27 21.55901317264984 28.38734205024617 28.199847751435957 21.85379702566803 28-29 21.596406654280408 28.467195263357326 28.289155725200093 21.64724235716217 30-31 21.902603612949658 28.822054637625044 27.812318754123886 21.46302299530141 32-33 21.486280235953966 28.652592092501028 28.224580132323634 21.63654753922137 34-35 21.665168896640118 28.29870942909126 28.213315469989443 21.822806204279175 36-37 22.050539880902353 28.589149631675774 27.77259958703097 21.587710900390903 38-39 21.845140395857683 28.705004445629818 27.903748788059513 21.546106370452986 40-41 21.68198538516047 28.458701701831785 28.083743116260372 21.775569796747373 42-43 21.65141632202684 28.475857766742795 28.054513449287843 21.818212461942515 44-45 21.788083440414653 28.595853260388676 27.923345866215115 21.69271743298156 46-47 21.74473338587202 28.748380465750216 27.801331316845175 21.705554831532588 48-49 21.657513192062115 28.46836063605757 27.93988718961028 21.934238982270035 50-51 21.67451972942028 28.429986953574193 27.916638299246966 21.97885501775856 52-53 21.674137049711835 28.445368134587383 27.90829890747727 21.972195908223508 54-55 21.7760012288722 28.739169288603193 27.744414097996795 21.740415384527818 56-57 21.61593459228586 28.51888876018179 27.771910393347692 22.093266254184655 58-59 21.510802348785045 28.219720140199716 28.07693317006515 22.192544340950093 60-61 21.819702465927758 28.539892286420898 27.729260966808322 21.91114428084302 62-63 21.627661724524422 28.57108746990043 27.751681340122218 22.04956946545293 64-65 21.66028669369063 28.35344803880143 27.863366467205037 22.122898800302902 66-67 21.54678287002537 28.340652194177913 27.794723839146368 22.31784109665035 68-69 22.06538644485752 28.460361941956602 27.618774917541323 21.855476695644555 70-71 21.804635001555706 28.267339896048334 27.78367804626279 22.144347056133178 72-73 21.64806652864438 28.400119546707153 27.940930679837994 22.01088324481047 74-75 21.663750156716823 28.384499405067455 27.82772592652169 22.12402451169403 76-77 21.685907978801733 28.42333902854882 27.6292467944453 22.261506198204145 78-79 21.84290027611791 28.423615266378256 27.723436509817063 22.010047947686775 80-81 21.609526093454207 28.326750558725134 27.851752879970054 22.211970467850612 82-83 21.550010489896653 28.30608793978282 27.95095429968223 22.1929472706383 84-85 21.663458084535367 28.167037052801742 27.806014626862343 22.36349023580055 86-87 21.80560139833517 28.266374127103166 27.773334570318347 22.154689904243313 88-89 21.595749650939375 28.150682420177333 27.862696315185335 22.39087161369796 90-91 21.554441842176814 28.046955842158184 27.844243119839128 22.55435919582587 92-93 21.687147245046184 28.188128582992363 27.73434756735379 22.390376604607667 94-95 21.63503749999538 27.960562815957466 27.86973930933181 22.534660374715347 96-97 21.621788049504236 28.005714881749306 27.845108578539595 22.527388490206867 98-99 21.61068471147352 27.959877331743648 27.830035389395718 22.599402567387113 100 21.745824803648453 27.896246307828008 27.71638737400634 22.641541514517197 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3402.0 1 2651.5 2 2241.5 3 2518.0 4 2296.0 5 2006.0 6 1876.5 7 1856.0 8 1948.0 9 1984.5 10 1739.0 11 1475.0 12 1384.5 13 1480.0 14 1631.5 15 1806.5 16 2102.5 17 2718.0 18 3572.0 19 4816.0 20 6708.0 21 9490.0 22 13814.0 23 20060.5 24 28313.5 25 38871.5 26 52664.5 27 69951.0 28 90683.0 29 111668.5 30 129476.5 31 145957.5 32 161592.5 33 176159.0 34 190055.0 35 201874.0 36 210993.0 37 218946.5 38 225948.0 39 230019.0 40 232080.5 41 232376.5 42 229621.0 43 227342.0 44 224136.0 45 220123.0 46 218215.5 47 214655.0 48 209407.5 49 206219.0 50 202374.0 51 194874.5 52 185823.0 53 178131.0 54 172075.0 55 164209.0 56 151907.0 57 137654.0 58 125005.0 59 113783.0 60 100486.5 61 84911.5 62 71487.0 63 59556.0 64 48689.0 65 39739.0 66 31905.5 67 25244.0 68 19896.0 69 15367.5 70 11628.0 71 8823.5 72 6945.0 73 5509.5 74 4210.0 75 3064.0 76 2261.0 77 1753.5 78 1388.5 79 1302.0 80 902.0 81 393.0 82 271.0 83 207.5 84 160.5 85 117.0 86 100.5 87 87.0 88 70.0 89 53.0 90 46.0 91 46.0 92 31.5 93 26.0 94 24.5 95 18.0 96 17.0 97 17.5 98 11.5 99 25.5 100 57.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.016514026499616273 2 0.0020402288781978923 3 0.0010348987063322644 4 0.0017888963352314853 5 0.0014932109905651243 6 0.0031934017223967013 7 11.743306090364102 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.921954740331348E-4 18-19 0.0 20-21 0.0 22-23 7.3921336166590305E-6 24-25 0.0 26-27 1.1827413786654449E-4 28-29 0.0 30-31 0.0 32-33 7.3921336166590305E-6 34-35 3.696066808329515E-5 36-37 2.217640084997709E-5 38-39 1.4784267233318061E-5 40-41 0.0 42-43 2.217640084997709E-5 44-45 9.60977370165674E-5 46-47 6.283313574160177E-4 48-49 4.5831228423285985E-4 50-51 3.7699881444961053E-4 52-53 2.143718748831119E-4 54-55 3.1786174551633834E-4 56-57 0.001426681788015193 58-59 3.622145472162925E-4 60-61 0.0012270941803653992 62-63 0.0021585030160644367 64-65 0.0010792515080322183 66-67 5.871978299061184 68-69 5.512565370485605 70-71 0.001929346873948007 72-73 9.38800969315697E-4 74-75 0.0022915614211642997 76-77 0.005544100212494274 78-79 5.5040865932272975 80-81 0.00495272952316155 82-83 0.004376143101062146 84-85 5.871837848522468 86-87 0.0028016186407137725 88-89 0.0011679571114321268 90-91 0.0027794422398637955 92-93 0.0032821073257966096 94-95 0.004132202691712398 96-97 0.0029938141147469073 98-99 0.005011866592094823 100 0.004627475644028553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6763947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.0432729393147 #Duplication Level Percentage of deduplicated Percentage of total 1 91.34681912962614 79.51126109638261 2 6.1233216467502665 10.659879147865947 3 1.3317718477199032 3.4776534130193695 4 0.5157419344734762 1.7956746387449973 5 0.24127402491838046 1.0500640402068804 6 0.13745770251424705 0.7178860990535237 7 0.08639750997612028 0.5264225429490021 8 0.061548319420999247 0.4285893733054528 9 0.036753750413557246 0.2879250055911643 >10 0.11783593155076286 1.4726529232617067 >50 0.001021847121388177 0.05698702174016442 >100 3.856989597301042E-5 0.005514731604385021 >500 1.7785618882228712E-5 0.009489966274801639 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.4784267233318061E-5 0.0 0.0 1.4784267233318061E-5 0.0 10-11 2.9568534466636122E-5 0.0 0.0 4.435280169995418E-5 0.0 12-13 2.9568534466636122E-5 0.0 0.0 6.652920254993127E-5 0.0 14-15 2.9568534466636122E-5 7.3921336166590305E-6 0.0 7.39213361665903E-5 0.0 16-17 2.9568534466636122E-5 1.4784267233318061E-5 0.0 7.39213361665903E-5 0.0 18-19 4.435280169995418E-5 1.4784267233318061E-5 0.0 7.39213361665903E-5 0.0 20-21 5.9137068933272244E-5 1.4784267233318061E-5 0.0 8.131346978324934E-5 0.0 22-23 5.9137068933272244E-5 1.4784267233318061E-5 0.0 1.8480334041647576E-4 0.0 24-25 5.9137068933272244E-5 1.4784267233318061E-5 0.0 3.7699881444961053E-4 0.0 26-27 5.9137068933272244E-5 1.4784267233318061E-5 0.0 8.057425642158343E-4 0.0 28-29 5.9137068933272244E-5 3.696066808329515E-5 0.0 0.0023359142228642537 0.0 30-31 5.9137068933272244E-5 4.435280169995418E-5 0.0 0.007451270685592303 0.0 32-33 5.9137068933272244E-5 4.435280169995418E-5 0.0 0.018968214860347072 0.0 34-35 5.9137068933272244E-5 4.435280169995418E-5 0.0 0.037714665712194374 0.0 36-37 5.9137068933272244E-5 5.9137068933272244E-5 0.0 0.06734972938138042 0.0 38-39 5.9137068933272244E-5 7.39213361665903E-5 0.0 0.10927791125507044 0.0 40-41 5.9137068933272244E-5 8.870560339990837E-5 0.0 0.1559961957123555 0.0 42-43 5.9137068933272244E-5 8.870560339990837E-5 0.0 0.2046290427763553 0.0 44-45 5.9137068933272244E-5 9.609773701656739E-5 0.0 0.2662129079367417 0.0 46-47 5.9137068933272244E-5 1.0348987063322643E-4 0.0 0.341716160697297 0.0 48-49 5.9137068933272244E-5 1.256662714832035E-4 0.0 0.4218764576363475 0.0 50-51 5.9137068933272244E-5 1.3305840509986256E-4 0.0 0.5058289191207441 0.0 52-53 5.9137068933272244E-5 1.3305840509986256E-4 0.0 0.5921616476297049 0.0 54-55 5.9137068933272244E-5 1.3305840509986256E-4 0.0 0.6885255014564722 0.0 56-57 5.9137068933272244E-5 1.4045053871652159E-4 0.0 0.7926658798479644 0.0 58-59 5.9137068933272244E-5 1.478426723331806E-4 0.0 0.914170380104989 0.0 60-61 5.9137068933272244E-5 1.478426723331806E-4 0.0 1.0410120008332413 0.0 62-63 7.39213361665903E-5 1.478426723331806E-4 0.0 1.167217898070461 0.0 64-65 7.39213361665903E-5 1.478426723331806E-4 0.0 1.3124363629697275 0.0 66-67 7.39213361665903E-5 1.478426723331806E-4 0.0 1.4797573073828048 0.0 68-69 7.39213361665903E-5 1.478426723331806E-4 0.0 1.6458141969474331 0.0 70-71 7.39213361665903E-5 1.478426723331806E-4 0.0 1.8483143052421906 0.0 72-73 7.39213361665903E-5 1.7741120679981673E-4 0.0 2.0699970002721786 0.0 74-75 7.39213361665903E-5 1.7741120679981673E-4 0.0 2.2749808654621333 0.0 76-77 7.39213361665903E-5 1.7741120679981673E-4 0.0 2.4947637821526394 0.0 78-79 7.39213361665903E-5 1.7741120679981673E-4 0.0 2.7539467710199386 0.0 80-81 7.39213361665903E-5 1.7741120679981673E-4 0.0 3.0671071195560815 0.0 82-83 7.39213361665903E-5 1.7741120679981673E-4 0.0 3.3951921858642593 0.0 84-85 7.39213361665903E-5 1.7741120679981673E-4 0.0 3.727269004325433 0.0 86-87 7.39213361665903E-5 1.9219547403313478E-4 0.0 4.121728038377592 0.0 88 7.39213361665903E-5 2.0697974126645285E-4 0.0 4.451498511150368 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6175 0.0 43.067482 1 GTATCAA 9790 0.0 34.963337 1 ATCAACG 10140 0.0 32.5388 3 TCAACGC 10245 0.0 31.955269 4 CAACGCA 11035 0.0 29.853043 5 TATCAAC 11870 0.0 28.055386 2 AACGCAG 12060 0.0 27.485249 6 GTACATG 16190 0.0 23.325972 1 ACGCAGA 14210 0.0 23.037865 7 CGCAGAG 14580 0.0 22.235407 8 TACATGG 16750 0.0 21.410995 2 ACATGGG 17240 0.0 19.82176 3 GTGGTAT 3900 0.0 18.919819 1 GCAGAGT 16930 0.0 18.668251 9 TAGACCG 390 2.0263542E-9 17.077675 5 GAGTACA 13055 0.0 16.83835 1 AGAGTAC 14510 0.0 16.81154 10-11 CAGAGTA 16145 0.0 16.25727 10-11 CATGGGG 12475 0.0 15.852595 4 GTGTTAG 3055 0.0 14.760143 1 >>END_MODULE