FastQCFastQC Report
Wed 25 May 2016
SRR2049488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6763947
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218400.32288839637566646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217920.32217875154846715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133650.1975917315732959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128250.056.385451
GTATCAA227450.040.3628541
GTGGTAT51700.034.1486781
ATCAACG288400.031.5068383
TCAACGC290450.031.3330654
TATCAAC297750.030.6122762
CAACGCA300950.030.2498345
TGGTATC56150.029.5830352
AACGCAG314900.028.9541196
ACGCAGA355450.025.5841457
CGCAGAG358000.025.3889718
GCAGAGT388500.023.1054299
GTACATG174850.022.2945081
TAGACCG2607.1486284E-1021.6918015
AGAGTAC360400.021.16437110-11
TACATGG177350.021.0141472
GAGTACT292350.020.96264812-13
CAGAGTA379250.020.79410-11
ACATGGG182500.019.7765393
AGTACTT305150.019.42878212-13
Produced by FastQC (version 0.11.5)