##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049488_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6763947
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654385819403966	31.0	31.0	34.0	30.0	34.0
2	31.931997840905613	33.0	31.0	34.0	30.0	34.0
3	31.908966465881534	33.0	31.0	34.0	30.0	34.0
4	35.58122542947188	37.0	35.0	37.0	33.0	37.0
5	35.717677415272476	37.0	35.0	37.0	33.0	37.0
6	35.801118193267925	37.0	35.0	37.0	35.0	37.0
7	35.7852379683046	37.0	35.0	37.0	33.0	37.0
8	35.77019364581065	37.0	35.0	37.0	33.0	37.0
9	37.49950036568885	39.0	37.0	39.0	35.0	39.0
10-11	37.455569654818405	39.0	37.0	39.0	34.5	39.0
12-13	37.44300117963668	39.0	37.0	39.0	34.0	39.0
14-15	38.70903468049055	40.0	38.0	41.0	34.0	41.0
16-17	38.713834097162504	40.0	38.0	41.0	34.5	41.0
18-19	38.78447583932872	40.0	38.0	41.0	35.0	41.0
20-21	38.81401465741823	40.0	38.0	41.0	35.0	41.0
22-23	38.78572451853925	40.0	38.0	41.0	35.0	41.0
24-25	38.74798442388742	40.0	38.0	41.0	34.0	41.0
26-27	38.64988105317798	40.0	38.0	41.0	34.0	41.0
28-29	38.56109339709492	40.0	38.0	41.0	34.0	41.0
30-31	38.4377314015027	40.0	38.0	41.0	34.0	41.0
32-33	38.28388602098745	40.0	38.0	41.0	34.0	41.0
34-35	38.16893390796823	40.0	38.0	41.0	34.0	41.0
36-37	38.02025814217646	40.0	37.5	41.0	33.0	41.0
38-39	37.921368322371535	40.0	37.0	41.0	33.0	41.0
40-41	37.789927463949674	40.0	37.0	41.0	33.0	41.0
42-43	37.64083123359778	40.0	37.0	41.0	33.0	41.0
44-45	37.44961625216756	40.0	37.0	41.0	33.0	41.0
46-47	37.197304916788966	40.0	37.0	41.0	32.0	41.0
48-49	36.95646824258085	40.0	37.0	41.0	31.0	41.0
50-51	36.741169468063546	40.0	36.0	41.0	31.0	41.0
52-53	36.63649352959152	40.0	36.0	41.0	31.0	41.0
54-55	36.50281048920105	39.5	35.5	41.0	30.5	41.0
56-57	36.328224925476206	39.0	35.0	41.0	30.0	41.0
58-59	36.15705556238096	39.0	35.0	41.0	30.0	41.0
60-61	35.966458637242425	39.0	35.0	41.0	30.0	41.0
62-63	35.759443561577285	38.5	35.0	40.5	29.5	41.0
64-65	35.48947352780854	38.0	35.0	40.0	29.0	41.0
66-67	35.23000054553946	37.5	35.0	40.0	29.0	41.0
68-69	34.91986439278723	37.0	34.0	40.0	28.5	41.0
70-71	34.632131209780326	36.5	34.0	39.0	28.0	41.0
72-73	34.32290096300281	36.0	34.0	39.0	28.0	41.0
74-75	33.98248810938347	35.5	34.0	39.0	28.0	40.5
76-77	33.694641604968226	35.0	34.0	37.5	28.0	40.0
78-79	33.374307856049136	35.0	34.0	37.0	27.0	39.0
80-81	33.07491417363264	35.0	34.0	37.0	27.0	39.0
82-83	32.79093759900839	35.0	33.0	36.0	27.0	39.0
84-85	32.524142486628	35.0	33.0	36.0	26.0	37.0
86-87	32.26293553157646	35.0	33.0	36.0	26.0	37.0
88-89	32.04648417558565	35.0	33.0	35.0	26.0	37.0
90-91	31.895851416340193	35.0	33.0	35.0	26.0	36.0
92-93	31.741354049639952	35.0	33.0	35.0	25.5	36.0
94-95	31.560525385547816	35.0	33.0	35.0	25.0	36.0
96-97	31.392157862857292	35.0	33.0	35.0	25.0	36.0
98-99	31.213433887048495	35.0	33.0	35.0	24.5	35.0
100	31.100860784391127	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5282365312852555
1101	2	0.5010248469693437
1101	3	0.5767538995074979
1101	4	0.4232579545981068
1101	5	0.3744595508987558
1101	6	0.38743462050879884
1101	7	0.3554104980828541
1101	8	0.3677190943836308
1101	9	0.4076076467931955
1101	10-11	0.4154736520648754
1101	12-13	0.39640677694480786
1101	14-15	0.5761908576752788
1101	16-17	0.5739305295666952
1101	18-19	0.5393703044892391
1101	20-21	0.5189186806884152
1101	22-23	0.5319184624410056
1101	24-25	0.5650807922425756
1101	26-27	0.5558947679128678
1101	28-29	0.573099710052638
1101	30-31	0.5476979383204537
1101	32-33	0.6079210150593823
1101	34-35	0.6398805974389958
1101	36-37	0.6433767282133473
1101	38-39	0.6321684407533894
1101	40-41	0.6591399172409353
1101	42-43	0.6076257722604765
1101	44-45	0.636066638343479
1101	46-47	0.6951272304116785
1101	48-49	0.7291429400586154
1101	50-51	0.8180743107664696
1101	52-53	0.7631316229785838
1101	54-55	0.7354846661754664
1101	56-57	0.6492580956582756
1101	58-59	0.695544281216101
1101	60-61	0.7164471720363466
1101	62-63	0.70118985007025
1101	64-65	0.7458699561772022
1101	66-67	0.7330427787494784
1101	68-69	0.7139528630672416
1101	70-71	0.6901457780713187
1101	72-73	0.6682824538284109
1101	74-75	0.6785014745933893
1101	76-77	0.6291988099282548
1101	78-79	0.6265915056413434
1101	80-81	0.6202972236537647
1101	82-83	0.6005717835173243
1101	84-85	0.596683599712982
1101	86-87	0.6511265875434091
1101	88-89	0.6490511608500569
1101	90-91	0.6514108290056946
1101	92-93	0.6673224924425725
1101	94-95	0.5780212733333592
1101	96-97	0.6448271602544189
1101	98-99	0.7217634690463015
1101	100	0.7507016845335812
1102	1	0.3318165407342839
1102	2	0.3928012019231204
1102	3	0.44154672547913165
1102	4	0.37042225627213554
1102	5	0.3005508489122306
1102	6	0.2941709474941163
1102	7	0.2939912206267081
1102	8	0.28509944485606553
1102	9	0.3839736651115331
1102	10-11	0.33191372706814803
1102	12-13	0.30951726709547955
1102	14-15	0.4366361611991465
1102	16-17	0.4289880756875277
1102	18-19	0.4087725311961776
1102	20-21	0.39431611009727874
1102	22-23	0.373708679621501
1102	24-25	0.3538208883231633
1102	26-27	0.33634671436723096
1102	28-29	0.3628947342074085
1102	30-31	0.40020162685073046
1102	32-33	0.37969945670950267
1102	34-35	0.4345639707182585
1102	36-37	0.4173293202393964
1102	38-39	0.4228004047343177
1102	40-41	0.42153328432321757
1102	42-43	0.43647665256445833
1102	44-45	0.4689630801475815
1102	46-47	0.5176874048864164
1102	48-49	0.49145566852767075
1102	50-51	0.5093221551796958
1102	52-53	0.43633283603912076
1102	54-55	0.37074101008995797
1102	56-57	0.35030206436960754
1102	58-59	0.39550794282968127
1102	60-61	0.4238451311127065
1102	62-63	0.4427148864296697
1102	64-65	0.4498271065409227
1102	66-67	0.44595186694310485
1102	68-69	0.4268815350743296
1102	70-71	0.4338632339172861
1102	72-73	0.40467863458511744
1102	74-75	0.43467204813617855
1102	76-77	0.39188404104038455
1102	78-79	0.3392839097721563
1102	80-81	0.3111038537948332
1102	82-83	0.3144884284125169
1102	84-85	0.35157088655758173
1102	86-87	0.36307433346961204
1102	88-89	0.3314570607315446
1102	90-91	0.338038039862564
1102	92-93	0.3663080466981441
1102	94-95	0.3683759927799777
1102	96-97	0.41644343068017164
1102	98-99	0.43484295324525135
1102	100	0.47643214394754807
1103	1	0.41531116319511696
1103	2	0.4323442757779219
1103	3	0.47651356480803386
1103	4	0.4070305389238058
1103	5	0.3124384681332728
1103	6	0.3085520039564287
1103	7	0.3387646073979411
1103	8	0.3370407861811273
1103	9	0.33822031278342024
1103	10-11	0.38086296501875694
1103	12-13	0.3279355100371717
1103	14-15	0.4694729802066675
1103	16-17	0.4438565418944478
1103	18-19	0.46458711056401114
1103	20-21	0.44985778040526725
1103	22-23	0.44014332664338696
1103	24-25	0.49712525548727626
1103	26-27	0.5179372156804973
1103	28-29	0.4928972118309005
1103	30-31	0.4998131444199885
1103	32-33	0.5022321938703982
1103	34-35	0.537412486625847
1103	36-37	0.573316292872768
1103	38-39	0.5648004518304788
1103	40-41	0.5880002287557318
1103	42-43	0.5686712636470972
1103	44-45	0.6158874318369456
1103	46-47	0.5667337076195267
1103	48-49	0.5642372388659496
1103	50-51	0.6206268223788385
1103	52-53	0.5378443995169775
1103	54-55	0.49464360609329106
1103	56-57	0.5845454345952774
1103	58-59	0.5274588921776626
1103	60-61	0.4920324658384132
1103	62-63	0.5188645884804686
1103	64-65	0.5057087471884856
1103	66-67	0.6016771361550184
1103	68-69	0.5792607894994717
1103	70-71	0.5956506266200066
1103	72-73	0.5640318611745911
1103	74-75	0.5443800590409751
1103	76-77	0.5668885095931984
1103	78-79	0.47747751112934367
1103	80-81	0.44594091985420903
1103	82-83	0.4516281922597756
1103	84-85	0.49169517170332
1103	86-87	0.500947183578063
1103	88-89	0.5227607849924496
1103	90-91	0.49657352890089257
1103	92-93	0.5181899900197422
1103	94-95	0.5332585066350326
1103	96-97	0.5000176547886745
1103	98-99	0.5216866964034992
1103	100	0.5349994990200244
1104	1	0.3385209265993403
1104	2	0.34150911176975285
1104	3	0.4007301662225764
1104	4	0.35429415006555587
1104	5	0.24431733715473314
1104	6	0.2631861339117094
1104	7	0.20524634652193186
1104	8	0.2168842765570389
1104	9	0.28118918723912856
1104	10-11	0.3376176879813144
1104	12-13	0.31564719310537015
1104	14-15	0.40379327081905103
1104	16-17	0.3700289217291868
1104	18-19	0.35246504522862665
1104	20-21	0.3765725609126278
1104	22-23	0.3960208092661972
1104	24-25	0.3810729793586205
1104	26-27	0.38079356270033315
1104	28-29	0.40737544558363226
1104	30-31	0.43214564167458036
1104	32-33	0.46167760277892
1104	34-35	0.45176847270504084
1104	36-37	0.476833143713705
1104	38-39	0.4580628487818217
1104	40-41	0.47034729307377177
1104	42-43	0.47697795473703763
1104	44-45	0.45772811910349276
1104	46-47	0.5698003932160347
1104	48-49	0.5814812391482675
1104	50-51	0.599252007324651
1104	52-53	0.5757942874991429
1104	54-55	0.5421094843020882
1104	56-57	0.6111873788151385
1104	58-59	0.6624906314539842
1104	60-61	0.6254880698089735
1104	62-63	0.6012717267743142
1104	64-65	0.5843139090928631
1104	66-67	0.5719364331832537
1104	68-69	0.5738368312880482
1104	70-71	0.594912002858095
1104	72-73	0.5976702263388631
1104	74-75	0.6477041245536128
1104	76-77	0.6215640993535629
1104	78-79	0.586342732132934
1104	80-81	0.5933436065241011
1104	82-83	0.6161044077954969
1104	84-85	0.6265033740399772
1104	86-87	0.6293027871275143
1104	88-89	0.5896160475699546
1104	90-91	0.5858062577128926
1104	92-93	0.6173508377042651
1104	94-95	0.6168493049471699
1104	96-97	0.6198664687941253
1104	98-99	0.6988680343466953
1104	100	0.7747173965277057
1105	1	0.32473610035714984
1105	2	0.3159452325723606
1105	3	0.420800461736885
1105	4	0.3336654566831285
1105	5	0.23058193020734308
1105	6	0.2631799133011725
1105	7	0.2458082858415338
1105	8	0.2594734343915448
1105	9	0.2746930391513871
1105	10-11	0.28481657601262356
1105	12-13	0.28418293872297795
1105	14-15	0.3640958469195823
1105	16-17	0.33887160217120993
1105	18-19	0.3942655118416667
1105	20-21	0.36428121826333637
1105	22-23	0.3636249484044143
1105	24-25	0.37087254643028444
1105	26-27	0.4028606321649235
1105	28-29	0.4149849662037681
1105	30-31	0.461132523901405
1105	32-33	0.42010112951651024
1105	34-35	0.4025923187822613
1105	36-37	0.44763328496826915
1105	38-39	0.47073136031096396
1105	40-41	0.49447904482029514
1105	42-43	0.4992657344522584
1105	44-45	0.5094594448446799
1105	46-47	0.5018285431412721
1105	48-49	0.5684848659510067
1105	50-51	0.5879036195658642
1105	52-53	0.5608127829807827
1105	54-55	0.5809422573477079
1105	56-57	0.6045359443003164
1105	58-59	0.5658590976623259
1105	60-61	0.5578783041005195
1105	62-63	0.5136198591144563
1105	64-65	0.5558282997281125
1105	66-67	0.5773104569186742
1105	68-69	0.571445249384368
1105	70-71	0.593723301160594
1105	72-73	0.5531852160207791
1105	74-75	0.538474145163633
1105	76-77	0.50227926426615
1105	78-79	0.43317227935299485
1105	80-81	0.4873414655132393
1105	82-83	0.5047809781349173
1105	84-85	0.5374210368247176
1105	86-87	0.5780754429226533
1105	88-89	0.5350235492115516
1105	90-91	0.531267596590407
1105	92-93	0.5158318514762286
1105	94-95	0.5762374372888068
1105	96-97	0.5807717015661034
1105	98-99	0.6785986232537624
1105	100	0.6660169349341203
1106	1	0.24879496314975924
1106	2	0.2529545342636439
1106	3	0.36734031442793125
1106	4	0.29558933218676486
1106	5	0.21789574739680262
1106	6	0.23433508536623293
1106	7	0.20583303272358933
1106	8	0.22410487417391778
1106	9	0.26595855814715463
1106	10-11	0.2665995412291622
1106	12-13	0.20329262863632636
1106	14-15	0.3224418082438447
1106	16-17	0.2968820533353096
1106	18-19	0.3046859680363383
1106	20-21	0.2853107789065845
1106	22-23	0.2326463319350509
1106	24-25	0.2826480783192693
1106	26-27	0.3136576666277975
1106	28-29	0.32091934218941987
1106	30-31	0.34760189214038917
1106	32-33	0.3885181442761052
1106	34-35	0.32292127016655314
1106	36-37	0.3138570619410146
1106	38-39	0.363195025980815
1106	40-41	0.37550102704074106
1106	42-43	0.3805145908320853
1106	44-45	0.3743029579850443
1106	46-47	0.38389293163876914
1106	48-49	0.38838374016742705
1106	50-51	0.4671502202391551
1106	52-53	0.45517397163583695
1106	54-55	0.37019949248998074
1106	56-57	0.4669868556993251
1106	58-59	0.4216503449593887
1106	60-61	0.4284146530487405
1106	62-63	0.43425508450799555
1106	64-65	0.4065045729670089
1106	66-67	0.39179863503861867
1106	68-69	0.40502477878871446
1106	70-71	0.41524822369474634
1106	72-73	0.40588735912832874
1106	74-75	0.38304550393220893
1106	76-77	0.401086405789421
1106	78-79	0.40862332929808076
1106	80-81	0.33284704232711704
1106	82-83	0.3486132149859529
1106	84-85	0.3724133543747641
1106	86-87	0.39911389833917355
1106	88-89	0.3823325858561333
1106	90-91	0.37927095563599167
1106	92-93	0.356147066211598
1106	94-95	0.3895677932747681
1106	96-97	0.4101721529606941
1106	98-99	0.4636448033462095
1106	100	0.44436597853748694
1107	1	0.20041678283773834
1107	2	0.22311556188925863
1107	3	0.31873065100069553
1107	4	0.25658684129852816
1107	5	0.2017180447001934
1107	6	0.2110121563957179
1107	7	0.16284112302774645
1107	8	0.20884133227122703
1107	9	0.18530710198405842
1107	10-11	0.19024508585599875
1107	12-13	0.18228962660668202
1107	14-15	0.25780586297688046
1107	16-17	0.19488245092094303
1107	18-19	0.233395739088742
1107	20-21	0.234044164221352
1107	22-23	0.2326509054163992
1107	24-25	0.30461800735464095
1107	26-27	0.29193735662405373
1107	28-29	0.30878582595897086
1107	30-31	0.30083347192196896
1107	32-33	0.28055111870790483
1107	34-35	0.27177308319111404
1107	36-37	0.3255674653101295
1107	38-39	0.28917901531990964
1107	40-41	0.26806166359068584
1107	42-43	0.30481140008006236
1107	44-45	0.32926957701376125
1107	46-47	0.31752846938308465
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2216	3	-1.225676141384362
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2216	80-81	-1.8717802596471884
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2216	84-85	-2.0649963708601966
2216	86-87	-2.195856464058302
2216	88-89	-2.0746139295765786
2216	90-91	-1.907045283499695
2216	92-93	-2.016739731422824
2216	94-95	-2.1318351620183336
2216	96-97	-2.179571225401393
2216	98-99	-2.320214953163962
2216	100	-2.349428081129645
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	366.0
3	38.0
4	58.0
5	171.0
6	483.0
7	1136.0
8	2786.0
9	4434.0
10	6312.0
11	8721.0
12	12421.0
13	17716.0
14	20779.0
15	26644.0
16	33205.0
17	45845.0
18	45759.0
19	25216.0
20	18282.0
21	18095.0
22	19206.0
23	20736.0
24	24080.0
25	29856.0
26	37792.0
27	49458.0
28	64155.0
29	84114.0
30	108771.0
31	140492.0
32	179695.0
33	234846.0
34	320782.0
35	466658.0
36	760073.0
37	1378193.0
38	1846464.0
39	707786.0
40	2323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.616426185434975	20.493500949040286	14.941197372125123	22.94887549339962
2	15.84287141603013	20.757183167557123	39.39128359840081	24.008661818011934
3	18.199979982102164	25.11620803652069	30.789714940108194	25.89409704126895
4	12.822236629041187	17.60268558893793	37.57078829968946	32.004289482331416
5	12.730946809660368	37.330667589613654	35.33340006070515	14.604985540020825
6	30.576165569927248	37.94074280619122	17.191662069916823	14.29142955396471
7	26.362403490151536	32.90031693033668	22.284207726642446	18.45307185286934
8	24.87971889785653	35.89846283538295	20.324316556590404	18.89750171017011
9	24.49132111255225	16.685031201475233	21.906260889543276	36.91738679642924
10-11	23.771105835098943	27.014249224602143	28.448641007979514	20.7660039323194
12-13	24.59486666510939	24.490563644357895	28.95001675057725	21.964552939955464
14-15	21.79948079120389	26.49313185625165	26.914032645591245	24.793354706953213
16-17	20.614960466130206	29.68529321711125	27.65077106606542	22.048975250693122
18-19	20.422935011170253	27.794555457043057	30.889967056217326	20.892542475569368
20-21	21.48143680014051	27.108476751813697	30.746877525799654	20.663208922246138
22-23	21.161970961629358	27.0069088359208	30.842731322406873	20.98838888004297
24-25	21.083932207038288	27.014278793136608	30.836728909910143	21.065060089914954
26-27	20.838358136159258	26.99846701933058	30.926476804150006	21.236698040360164
28-29	20.90261740937974	27.00984928902385	31.038618024405896	21.048915277190513
30-31	21.198260035859317	27.353665933242237	30.60485154161429	20.843222489284155
32-33	20.84920547857715	27.167928306937995	30.94790378279188	21.03496243169298
34-35	21.022037872266	26.892153353655786	30.856406769597694	21.22940200448052
36-37	21.448275688736178	27.212284484192438	30.29113770406539	21.04830212300599
38-39	21.2297050819588	27.293657091044622	30.429503661102014	21.04713416589456
40-41	21.133149032658004	27.115477102348674	30.45848082487932	21.292893040114006
42-43	21.182550661618134	27.197381942821252	30.258560571216776	21.361506824343834
44-45	21.36045714137027	27.3806625037127	29.98449721737914	21.27438313753789
46-47	21.452060461147905	27.59791731070631	29.544103465033068	21.405918763112723
48-49	21.481739877618793	27.471282669719322	29.337308527107027	21.709668925554855
50-51	21.572411788560732	27.526634966240866	29.12702450211393	21.773928743084475
52-53	21.533285225327756	27.648642131583824	29.054308083726855	21.76376455936157
54-55	21.611915350608157	27.945362374956517	28.866126538247567	21.576595736187762
56-57	21.371774497937373	27.727797098351008	28.921264462894225	21.979163940817394
58-59	21.30618747578608	27.35498647642617	29.291257397050042	22.047568650737713
60-61	21.602623438652017	27.63911367135195	28.97884179163438	21.779421098361652
62-63	21.392834686611234	27.664195180713275	29.06153019827033	21.881439934405165
64-65	21.34985682176398	27.42509661888244	29.19536477739994	22.029681781953645
66-67	21.273370415232407	27.459455255932667	29.048889649785842	22.218284679049084
68-69	21.640168085291027	27.650386675117357	28.85396647844816	21.855478761143456
70-71	21.58338910698147	27.402558003485243	28.910390634344118	22.103662255189167
72-73	21.398467492427127	27.57483167742148	29.013799191507562	22.01290163864383
74-75	21.4436260366913	27.537117011709288	28.852776344935876	22.166480606663537
76-77	21.471575693895886	27.596882411999974	28.65111893987342	22.280422954230716
78-79	21.530014929181746	27.60434977632839	28.77194691728216	22.0936883772077
80-81	21.32425438654202	27.47602095646508	28.85522714837106	22.344497508621842
82-83	21.190154420603765	27.50864179680183	28.950200393883495	22.35100338871091
84-85	21.247098772358804	27.3800415644889	28.78592928064043	22.58693038251187
86-87	21.364355954349723	27.464057223401845	28.735940529389204	22.435646292859225
88-89	21.15055565612974	27.384614042657528	28.77893440960931	22.685895891603423
90-91	20.994170263435137	27.260826600819467	28.807624311385283	22.937378824360113
92-93	21.051895083559078	27.39993675243724	28.695720824844024	22.85244733915966
94-95	20.901616743582295	27.16243797037362	28.85421956695134	23.081725719092745
96-97	20.86694505936734	27.23393805672473	28.81606174738372	23.083055136524216
98-99	20.820339192536427	27.176266634890347	28.77366259281195	23.229731579761282
100	20.893443954765992	27.091083789717956	28.682130544309903	23.33334171120615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1829.0
1	1312.5
2	1853.0
3	2909.5
4	1885.0
5	968.5
6	2069.0
7	5353.0
8	8598.0
9	9665.0
10	7642.0
11	4917.5
12	4441.0
13	4807.5
14	5481.0
15	6231.0
16	7027.0
17	7994.5
18	9093.5
19	10392.0
20	12032.5
21	14513.5
22	18312.5
23	23651.0
24	30633.5
25	39697.5
26	52121.0
27	67658.0
28	87009.0
29	107604.5
30	125130.0
31	140779.0
32	155449.0
33	169995.5
34	183704.5
35	196018.5
36	205536.0
37	212631.0
38	220759.0
39	225989.5
40	228417.5
41	229931.0
42	227472.5
43	225009.0
44	223018.0
45	219487.0
46	216700.0
47	212764.5
48	206841.0
49	204694.5
50	202303.0
51	194766.5
52	185084.0
53	177223.5
54	171370.0
55	163075.5
56	151232.0
57	137329.0
58	125549.5
59	116154.5
60	102940.0
61	86680.5
62	73339.0
63	61333.5
64	50101.5
65	40625.5
66	32193.5
67	25353.0
68	19851.0
69	15220.0
70	11436.0
71	8511.0
72	6614.0
73	5193.5
74	3863.5
75	2698.5
76	1925.0
77	1453.5
78	1191.0
79	1051.0
80	661.5
81	232.0
82	121.0
83	57.5
84	28.0
85	16.5
86	15.0
87	13.0
88	7.0
89	4.0
90	3.0
91	2.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058545698243939515
2	1.3305840509986256E-4
3	0.0
4	0.11660351566917955
5	0.0015671323267317143
6	0.0016262693956649867
7	0.0
8	0.0
9	5.9137068933272244E-5
10-11	0.0
12-13	1.4784267233318061E-5
14-15	2.217640084997709E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.0348987063322644E-4
30-31	2.143718748831119E-4
32-33	1.4784267233318061E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.587246765830661E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.921954740331348E-4
80-81	2.956853446663612E-4
82-83	2.661168101997251E-4
84-85	0.0
86-87	1.4784267233318061E-5
88-89	9.60977370165674E-5
90-91	7.39213361665903E-5
92-93	7.39213361665903E-4
94-95	6.209392237993585E-4
96-97	7.3921336166590305E-6
98-99	1.4784267233318061E-5
100	0.00144885818886517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6763947.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.23130657186639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53688999171499	42.658436020293195
2	14.92868832469718	17.087766764531736
3	4.598245044442708	7.894907154931981
4	2.0742570248924186	4.748497588018619
5	1.0651921410359997	3.048116899078701
6	0.6352303645614892	2.181303824278622
7	0.42243979927937614	1.6923747162500975
8	0.2981219531134593	1.3649527115551972
9	0.22063792505304666	1.1364657057082255
>10	1.1282932922070288	11.376799099635495
>50	0.06366927124683172	2.4796975804841233
>100	0.026964776867134572	2.624741675109406
>500	8.531038678043271E-4	0.31679105805801816
>1k	4.1358961741245137E-4	0.4532413663361886
>5k	2.584935076279993E-5	0.09269871012575474
>10k+	7.754805228839978E-5	0.8432091256044756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21840	0.32288839637566646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21792	0.32217875154846715	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13365	0.1975917315732959	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9568534466636122E-5	0.0	1.4784267233318061E-5	0.0
3	0.0	2.9568534466636122E-5	0.0	1.4784267233318061E-5	0.0
4	0.0	2.9568534466636122E-5	0.0	2.9568534466636122E-5	0.0
5	0.0	4.435280169995418E-5	0.0	2.9568534466636122E-5	0.0
6	0.0	4.435280169995418E-5	0.0	2.9568534466636122E-5	0.0
7	0.0	4.435280169995418E-5	0.0	4.435280169995418E-5	0.0
8	0.0	4.435280169995418E-5	0.0	4.435280169995418E-5	0.0
9	1.4784267233318061E-5	4.435280169995418E-5	0.0	5.9137068933272244E-5	0.0
10-11	1.4784267233318061E-5	5.9137068933272244E-5	0.0	5.9137068933272244E-5	0.0
12-13	1.4784267233318061E-5	5.9137068933272244E-5	0.0	8.131346978324934E-5	0.0
14-15	1.4784267233318061E-5	7.39213361665903E-5	0.0	1.1827413786654449E-4	0.0
16-17	1.4784267233318061E-5	1.1827413786654449E-4	0.0	1.3305840509986256E-4	0.0
18-19	2.217640084997709E-5	1.478426723331806E-4	0.0	1.3305840509986256E-4	0.0
20-21	2.9568534466636122E-5	1.478426723331806E-4	0.0	1.4045053871652159E-4	0.0
22-23	2.9568534466636122E-5	1.478426723331806E-4	0.0	2.4394040934974803E-4	0.0
24-25	2.9568534466636122E-5	1.5523480594983966E-4	0.0	4.509201506162009E-4	0.0
26-27	2.9568534466636122E-5	2.0697974126645285E-4	0.0	9.018403012324018E-4	0.0
28-29	2.9568534466636122E-5	2.0697974126645285E-4	0.0	0.00243940409349748	0.0
30-31	2.9568534466636122E-5	2.0697974126645285E-4	0.0	0.007650858293242097	0.0
32-33	2.9568534466636122E-5	2.0697974126645285E-4	0.0	0.019241723804163457	0.0
34-35	2.9568534466636122E-5	2.143718748831119E-4	0.0	0.03815819372919392	0.0
36-37	2.9568534466636122E-5	2.4394040934974803E-4	0.0	0.06811851127751296	0.0
38-39	2.9568534466636122E-5	2.7350894381638415E-4	0.0	0.11040151556480263	0.0
40-41	2.9568534466636122E-5	2.8090107743304317E-4	0.0	0.15719372135825427	0.0
42-43	2.9568534466636122E-5	2.8090107743304317E-4	0.0	0.20620356723670366	0.0
44-45	2.9568534466636122E-5	2.8090107743304317E-4	0.0	0.2682900974830229	0.0
46-47	2.9568534466636122E-5	2.8090107743304317E-4	0.0	0.3442590546614277	0.0
48-49	2.9568534466636122E-5	2.8090107743304317E-4	0.0	0.42465589987621133	0.0
50-51	2.9568534466636122E-5	3.1046961189967927E-4	0.0	0.5086674984295412	0.0
52-53	2.9568534466636122E-5	3.1046961189967927E-4	0.0	0.5949632662704187	0.0
54-55	2.9568534466636122E-5	3.1046961189967927E-4	0.0	0.6947496779616991	0.0
56-57	2.9568534466636122E-5	3.1046961189967927E-4	0.0	0.8145392032196586	0.0
58-59	2.9568534466636122E-5	3.2525387913299737E-4	0.0	0.9537330792213481	0.0
60-61	4.435280169995418E-5	3.2525387913299737E-4	0.0	1.0995946597452642	0.0
62-63	5.9137068933272244E-5	3.2525387913299737E-4	0.0	1.2455745144070467	0.0
64-65	5.9137068933272244E-5	3.2525387913299737E-4	0.0	1.412459322936741	0.0
66-67	5.9137068933272244E-5	3.2525387913299737E-4	0.0	1.6101471522470534	0.0
68-69	5.9137068933272244E-5	3.2525387913299737E-4	0.0	1.827801134455962	0.0
70-71	5.9137068933272244E-5	3.2525387913299737E-4	0.0	2.0664635604034154	0.0
72-73	7.39213361665903E-5	3.5482241359963346E-4	0.0	2.319488901968037	0.0
74-75	8.870560339990837E-5	3.5482241359963346E-4	0.0	2.58200574309645	0.0
76-77	8.870560339990837E-5	3.5482241359963346E-4	0.0	2.8728640245111325	0.0
78-79	8.870560339990837E-5	3.5482241359963346E-4	0.0	3.20386898359789	0.0
80-81	8.870560339990837E-5	3.5482241359963346E-4	0.0	3.564538574888301	0.0
82-83	8.870560339990837E-5	3.5482241359963346E-4	0.0	3.9408129602434796	0.0
84-85	8.870560339990837E-5	3.5482241359963346E-4	0.0	4.3227201514145515	0.0
86-87	8.870560339990837E-5	3.696066808329515E-4	0.0	4.732414372850645	0.0
88	8.870560339990837E-5	3.843909480662696E-4	0.0	5.065237796807101	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12825	0.0	56.38545	1
GTATCAA	22745	0.0	40.362854	1
GTGGTAT	5170	0.0	34.148678	1
ATCAACG	28840	0.0	31.506838	3
TCAACGC	29045	0.0	31.333065	4
TATCAAC	29775	0.0	30.612276	2
CAACGCA	30095	0.0	30.249834	5
TGGTATC	5615	0.0	29.583035	2
AACGCAG	31490	0.0	28.954119	6
ACGCAGA	35545	0.0	25.584145	7
CGCAGAG	35800	0.0	25.388971	8
GCAGAGT	38850	0.0	23.105429	9
GTACATG	17485	0.0	22.294508	1
TAGACCG	260	7.1486284E-10	21.691801	5
AGAGTAC	36040	0.0	21.164371	10-11
TACATGG	17735	0.0	21.014147	2
GAGTACT	29235	0.0	20.962648	12-13
CAGAGTA	37925	0.0	20.794	10-11
ACATGGG	18250	0.0	19.776539	3
AGTACTT	30515	0.0	19.428782	12-13
>>END_MODULE