FastQCFastQC Report
Wed 25 May 2016
SRR2049487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6609529
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62950.043.2983631
GTATCAA94650.034.6970861
TCAACGC99050.031.9589734
ATCAACG99450.031.7789233
CAACGCA109050.028.9810815
TATCAAC115600.027.9152492
AACGCAG116650.027.2241676
ACGCAGA135300.023.1306237
GTACATG162500.022.4482351
CGCAGAG139700.022.3994338
TACATGG163950.021.276222
ACATGGG166550.020.1136973
GCAGAGT162950.018.5374289
GTGGTAT38400.017.2113151
GAGTACA126600.016.875551
CATGGGG118550.016.6780224
AGAGTAC142850.016.38176710-11
CAGAGTA160850.016.2634610-11
AATACCG5700.016.1753145
TACGACC5757.2759576E-1215.1439754
Produced by FastQC (version 0.11.5)