##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049487_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6609529
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.76341007052091	31.0	31.0	34.0	28.0	34.0
2	30.954205360170143	31.0	31.0	34.0	28.0	34.0
3	31.049090638682422	31.0	31.0	34.0	28.0	34.0
4	34.721846140625146	37.0	35.0	37.0	32.0	37.0
5	34.749604850814634	37.0	35.0	37.0	32.0	37.0
6	34.84739563136798	37.0	35.0	37.0	32.0	37.0
7	30.89648097466552	37.0	33.0	37.0	2.0	37.0
8	32.65471654636813	37.0	33.0	37.0	17.0	37.0
9	35.50928863463645	39.0	35.0	39.0	30.0	39.0
10-11	36.06224134881623	39.0	36.0	39.0	32.0	39.0
12-13	36.21922522769776	39.0	37.0	39.0	32.0	39.0
14-15	37.427590982655495	40.0	38.0	41.0	32.0	41.0
16-17	37.44276112564148	40.0	38.0	41.0	32.0	41.0
18-19	37.44700257764207	40.0	38.0	41.0	32.0	41.0
20-21	37.50835891634638	40.0	38.0	41.0	32.0	41.0
22-23	37.46676359238306	40.0	38.0	41.0	32.0	41.0
24-25	37.4495110014647	40.0	38.0	41.0	32.0	41.0
26-27	37.467649888517016	40.0	38.0	41.0	32.0	41.0
28-29	37.44468471202713	40.0	38.0	41.0	32.0	41.0
30-31	37.35664144903517	40.0	37.0	41.0	31.0	41.0
32-33	37.30057815012235	40.0	37.0	41.0	31.0	41.0
34-35	37.23127374129079	40.0	37.0	41.0	31.0	41.0
36-37	37.05754025740714	40.0	37.0	41.0	30.5	41.0
38-39	36.92908291952422	40.0	37.0	41.0	30.0	41.0
40-41	36.84281345917387	40.0	37.0	41.0	30.0	41.0
42-43	36.714788451643074	40.0	36.5	41.0	30.0	41.0
44-45	36.587626818794504	40.0	36.0	41.0	30.0	41.0
46-47	36.4331215582835	40.0	36.0	41.0	30.0	41.0
48-49	36.34673249788298	40.0	36.0	41.0	30.0	41.0
50-51	36.21766029016591	39.5	35.5	41.0	30.0	41.0
52-53	36.08752045720656	39.0	35.0	41.0	29.0	41.0
54-55	35.96099767472084	39.0	35.0	41.0	29.0	41.0
56-57	35.75948936754797	39.0	35.0	41.0	28.5	41.0
58-59	35.579820286740556	39.0	35.0	41.0	28.0	41.0
60-61	35.358305939802975	38.5	35.0	40.0	28.0	41.0
62-63	35.112588884926595	38.0	34.0	40.0	28.0	41.0
64-65	34.76256432190554	38.0	34.0	40.0	27.0	41.0
66-67	31.250769911138903	36.0	31.0	40.0	6.5	41.0
68-69	31.60231387138176	36.0	30.5	39.5	11.0	41.0
70-71	31.754992224105532	35.5	30.0	39.0	15.5	41.0
72-73	32.98572175112629	35.0	32.5	39.0	24.5	40.5
74-75	33.051358727679386	35.0	33.0	38.5	25.0	40.0
76-77	32.723718361777365	35.0	33.0	37.5	24.5	39.5
78-79	29.314179497510338	35.0	29.0	37.0	6.5	39.0
80-81	31.072103549284677	35.0	30.0	36.0	19.0	39.0
82-83	31.440007828091836	35.0	32.0	36.0	20.5	37.5
84-85	29.565180514375534	35.0	30.0	35.5	11.0	37.0
86-87	29.152666627228655	34.0	28.0	35.0	10.5	37.0
88-89	30.33202547412985	34.0	30.5	35.0	16.0	36.0
90-91	30.431317571948014	35.0	31.5	35.0	8.5	36.0
92-93	30.218104724254935	35.0	32.0	35.0	2.0	36.0
94-95	29.94524103003406	35.0	31.0	35.0	2.0	36.0
96-97	29.689508208527414	35.0	31.0	35.0	2.0	35.0
98-99	29.430777593985894	34.5	31.0	35.0	2.0	35.0
100	29.180091803818396	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.15122517447053951
1101	2	0.15744531576205034
1101	3	0.1726926218861209
1101	4	0.24941087530044115
1101	5	0.22484652804744343
1101	6	0.251308843693991
1101	7	-12.241571354478317
1101	8	-6.678298707972843
1101	9	-2.2756710697113007
1101	10-11	-0.23408170742995082
1101	12-13	0.24305326492854107
1101	14-15	0.4280594417265746
1101	16-17	0.46739187501574264
1101	18-19	0.48318953517307506
1101	20-21	0.4410986275238997
1101	22-23	0.39039848517636244
1101	24-25	0.4305342298322046
1101	26-27	0.39950052352545384
1101	28-29	0.3721825626504085
1101	30-31	0.45338905740414503
1101	32-33	0.474778749090234
1101	34-35	0.47418922081092774
1101	36-37	0.5347954535660548
1101	38-39	0.5363995398559425
1101	40-41	0.5435229300199893
1101	42-43	0.47159886096584813
1101	44-45	0.37538053553195994
1101	46-47	0.4343345911395389
1101	48-49	0.311290654509385
1101	50-51	0.39396039994135634
1101	52-53	0.3736097871398698
1101	54-55	0.2899596259788879
1101	56-57	0.28137706289631126
1101	58-59	0.2775429114601522
1101	60-61	0.27912061966648594
1101	62-63	0.2951785294150966
1101	64-65	0.13787699632197814
1101	66-67	-10.405522330790319
1101	68-69	-8.313920197017417
1101	70-71	-6.858623543069477
1101	72-73	-1.7289525808948625
1101	74-75	-0.41377858261200373
1101	76-77	-0.14095903068859172
1101	78-79	3.0008871723548296
1101	80-81	0.8692865818482147
1101	82-83	0.2130760333416255
1101	84-85	-5.491985245452437
1101	86-87	-5.996993562279286
1101	88-89	-1.4857603928138658
1101	90-91	-0.15546175926122174
1101	92-93	0.18044416690306875
1101	94-95	0.2615930977682126
1101	96-97	0.3715773212957778
1101	98-99	0.4018457999927669
1101	100	0.39178700293165747
1102	1	0.05195515164315623
1102	2	0.06580963574716137
1102	3	0.05230350644852422
1102	4	0.10354234471916612
1102	5	0.07443280767105875
1102	6	0.12464561312624767
1102	7	-12.419831766958055
1102	8	-6.779515894851002
1102	9	-2.521665683754712
1102	10-11	-0.4398526574450301
1102	12-13	0.13262139232945458
1102	14-15	0.27177589558231574
1102	16-17	0.298252581957712
1102	18-19	0.28153002494205026
1102	20-21	0.2625881883560055
1102	22-23	0.3033576745815978
1102	24-25	0.3072047072236401
1102	26-27	0.3089466038210702
1102	28-29	0.3194794737408273
1102	30-31	0.26165755476949926
1102	32-33	0.24658783398793815
1102	34-35	0.32696357879419224
1102	36-37	0.4648918257272143
1102	38-39	0.4161792652673668
1102	40-41	0.3225303311120058
1102	42-43	0.35422739955718185
1102	44-45	0.3066469259763096
1102	46-47	0.33552147322102144
1102	48-49	0.20030162739512747
1102	50-51	0.29114179463326195
1102	52-53	0.3059592452532769
1102	54-55	0.24485188453185458
1102	56-57	0.2415629120292948
1102	58-59	0.2367692664831864
1102	60-61	0.1634138483335974
1102	62-63	0.16943590467550962
1102	64-65	0.028109701410919286
1102	66-67	-10.53316907254625
1102	68-69	-6.94430563517097
1102	70-71	-6.472053324075198
1102	72-73	-1.2911070268696676
1102	74-75	-0.35925074236652677
1102	76-77	-0.16997435482425516
1102	78-79	2.9698811017931703
1102	80-81	0.8525734228065147
1102	82-83	0.21011588618354082
1102	84-85	-5.595870531366341
1102	86-87	-6.091336139449055
1102	88-89	-1.502201880494134
1102	90-91	-0.22097098308154983
1102	92-93	0.15785113459219602
1102	94-95	0.16813081244775674
1102	96-97	0.279688954794306
1102	98-99	0.28254592273119883
1102	100	0.40556445453099244
1103	1	0.0530148608207881
1103	2	0.06430774390479854
1103	3	0.10558587230967476
1103	4	0.12816713791988832
1103	5	0.1451640963692995
1103	6	0.15503723481972287
1103	7	-12.27413254666553
1103	8	-6.702689415252188
1103	9	-2.4004074924386103
1103	10-11	-0.40318138699416295
1103	12-13	0.10919324427021593
1103	14-15	0.26809044857326114
1103	16-17	0.31397173047378857
1103	18-19	0.34050742026483505
1103	20-21	0.33322212653689576
1103	22-23	0.37239982291958995
1103	24-25	0.4593362883444456
1103	26-27	0.3316158049887008
1103	28-29	0.29135946628186105
1103	30-31	0.2815073817162528
1103	32-33	0.3124519517002824
1103	34-35	0.3261269773225308
1103	36-37	0.36202741378563985
1103	38-39	0.39361188290099136
1103	40-41	0.38871035810882404
1103	42-43	0.3552533627062573
1103	44-45	0.2994212753812633
1103	46-47	0.32854412693644264
1103	48-49	0.3004214161178922
1103	50-51	0.3187213171818044
1103	52-53	0.2554651530666092
1103	54-55	0.24726680647076904
1103	56-57	0.2824671959037346
1103	58-59	0.25671412108491154
1103	60-61	0.22204887955028596
1103	62-63	0.11319934178916924
1103	64-65	0.02083283492646615
1103	66-67	-10.449656185176686
1103	68-69	-8.393208211741548
1103	70-71	-6.983355614288463
1103	72-73	-1.8487601325366754
1103	74-75	-0.6581611585976646
1103	76-77	-0.30300587735730744
1103	78-79	2.8699280429705496
1103	80-81	0.7103684973058719
1103	82-83	0.028761744519908206
1103	84-85	-5.707678205458425
1103	86-87	-6.09267649556967
1103	88-89	-1.6622537771112675
1103	90-91	-0.37930125152701066
1103	92-93	-0.02330036959024895
1103	94-95	-0.015202940291281664
1103	96-97	0.1291260962024232
1103	98-99	0.1610924980577515
1103	100	0.19846807768012198
1104	1	0.004698502572654917
1104	2	0.0030458443397129997
1104	3	0.01914472096967401
1104	4	0.06526230161309599
1104	5	0.0903142418237195
1104	6	0.11340288878525229
1104	7	-12.281024963287663
1104	8	-6.739792214672242
1104	9	-2.5168467080909807
1104	10-11	-0.48613910328204923
1104	12-13	0.11441572640215014
1104	14-15	0.2980404036902158
1104	16-17	0.19880589576175822
1104	18-19	0.21616254144495173
1104	20-21	0.2754412128285324
1104	22-23	0.29437477588319894
1104	24-25	0.3593901191896407
1104	26-27	0.3083252173186679
1104	28-29	0.3391102970787827
1104	30-31	0.2966896661713676
1104	32-33	0.3021170159962878
1104	34-35	0.27864296475425476
1104	36-37	0.36373675665313954
1104	38-39	0.3648255219968135
1104	40-41	0.37306878777117447
1104	42-43	0.325252694837431
1104	44-45	0.31811925120359774
1104	46-47	0.35659847170103376
1104	48-49	0.29781892989641534
1104	50-51	0.3117607450085913
1104	52-53	0.321607743370663
1104	54-55	0.3167419336051083
1104	56-57	0.3307912156055508
1104	58-59	0.3452944799579001
1104	60-61	0.3269917160499247
1104	62-63	0.23865308353715875
1104	64-65	0.10914445634836056
1104	66-67	-10.428317431300556
1104	68-69	-8.370342518311041
1104	70-71	-6.8370756385293205
1104	72-73	-1.7601298378624506
1104	74-75	-0.5110704374156114
1104	76-77	-0.20100951039909631
1104	78-79	2.9500790636521614
1104	80-81	0.8092517671093447
1104	82-83	0.12956323857026675
1104	84-85	-5.568744663346621
1104	86-87	-6.038364450375219
1104	88-89	-1.5925851944122833
1104	90-91	-0.3029411634094892
1104	92-93	0.04694097494805405
1104	94-95	0.10784812018459888
1104	96-97	0.1769123425706951
1104	98-99	0.24184664854352178
1104	100	0.28225080235237954
1105	1	-0.0025817943909025587
1105	2	0.014674623144717458
1105	3	0.002896211033490914
1105	4	0.09554312807865273
1105	5	0.07673565999788678
1105	6	0.10088425903719411
1105	7	-12.319949799285837
1105	8	-6.777882464819669
1105	9	-2.600416099873165
1105	10-11	-0.5545577714747054
1105	12-13	0.023799235409214248
1105	14-15	0.12351916767358517
1105	16-17	0.24472439290519077
1105	18-19	0.23158276630395846
1105	20-21	0.23302825673152938
1105	22-23	0.211728062271888
1105	24-25	0.26197543916939026
1105	26-27	0.2524189768587064
1105	28-29	0.26307181108474964
1105	30-31	0.2560861085531414
1105	32-33	0.281188660808688
1105	34-35	0.29469656549426304
1105	36-37	0.36841823141496377
1105	38-39	0.3457591441570784
1105	40-41	0.37123253693607694
1105	42-43	0.2908540461973246
1105	44-45	0.2384269485920143
1105	46-47	0.1899147758276456
1105	48-49	0.21824723080650443
1105	50-51	0.27238112746989884
1105	52-53	0.27014895035078723
1105	54-55	0.23565318995486706
1105	56-57	0.21969060358910042
1105	58-59	0.21960338978107075
1105	60-61	0.10023063798638532
1105	62-63	0.02977243551582376
1105	64-65	-0.09681458814939958
1105	66-67	-10.493110241323897
1105	68-69	-8.51928786757378
1105	70-71	-6.972437655696499
1105	72-73	-1.968683675227119
1105	74-75	-0.7274018785680454
1105	76-77	-0.43213079290318746
1105	78-79	2.7241106485807407
1105	80-81	0.6343618173290757
1105	82-83	-0.07048401130600723
1105	84-85	-5.6692849714690645
1105	86-87	-6.166397137129358
1105	88-89	-1.7205660627355144
1105	90-91	-0.4750086530296045
1105	92-93	-0.10846789153211844
1105	94-95	-0.03797983741036148
1105	96-97	0.08765623599526506
1105	98-99	0.13572777573966377
1105	100	0.21392985540497378
1106	1	-0.05827100506052574
1106	2	-0.04592858490508789
1106	3	-0.0494251667255341
1106	4	-0.07456761930520628
1106	5	-0.05367875194141192
1106	6	0.0019373986479465088
1106	7	-12.36989344132262
1106	8	-6.919565709762782
1106	9	-2.7683735621826244
1106	10-11	-0.6482984960210132
1106	12-13	-0.07774053589642449
1106	14-15	5.542482059865961E-4
1106	16-17	0.059004973386080906
1106	18-19	0.07873908084885528
1106	20-21	0.07624441987649533
1106	22-23	0.019769405345314794
1106	24-25	0.07844288033388125
1106	26-27	0.06285131725344684
1106	28-29	0.031176730110800577
1106	30-31	0.051992475224551526
1106	32-33	0.0814980316845606
1106	34-35	0.1335052783495172
1106	36-37	0.22091460095639093
1106	38-39	0.1744801495488062
1106	40-41	0.1590313138640198
1106	42-43	0.09272744254037235
1106	44-45	-0.017609302027373985
1106	46-47	0.10576395051157306
1106	48-49	0.06606585873846882
1106	50-51	0.10378450207925738
1106	52-53	0.09422885600793052
1106	54-55	0.0625289166598364
1106	56-57	0.10124030039674636
1106	58-59	0.0808528746484356
1106	60-61	0.053593426889932516
1106	62-63	7.000207457537044E-4
1106	64-65	-0.15506006786053206
1106	66-67	-10.553602450556053
1106	68-69	-8.508484340387046
1106	70-71	-6.97055811149076
1106	72-73	-1.8919251565278046
1106	74-75	-0.6982672186046415
1106	76-77	-0.4057291721977947
1106	78-79	2.7944198140168126
1106	80-81	0.6106285376758365
1106	82-83	-0.12926309790225687
1106	84-85	-5.73730647273727
1106	86-87	-6.202714294480302
1106	88-89	-1.8028144235098829
1106	90-91	-0.5581758969718891
1106	92-93	-0.199651043874006
1106	94-95	-0.12050032926390841
1106	96-97	-0.029628579611785
1106	98-99	0.001129444278799241
1106	100	0.10894413807051251
1107	1	-0.07511457499193952
1107	2	-0.06694509612545829
1107	3	-0.07390033938969509
1107	4	-0.027349144426857208
1107	5	-0.08054308041490543
1107	6	-0.07674860580703324
1107	7	-12.393989298129764
1107	8	-6.871949001788185
1107	9	-2.7483546038595605
1107	10-11	-0.649826487932728
1107	12-13	-0.0024652741923887334
1107	14-15	0.08779444896401856
1107	16-17	0.09399109078426449
1107	18-19	0.10443589072630033
1107	20-21	0.07898039796312162
1107	22-23	0.17220436963225438
1107	24-25	0.20757284232759332
1107	26-27	0.09195603136223696
1107	28-29	0.08686108038114071
1107	30-31	0.09356191325743168
1107	32-33	0.1341652503685964
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2212	72-73	0.3284675253415159
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2212	82-83	-0.11449516838528595
2212	84-85	1.2788512182219733
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2213	84-85	1.7209350475240228
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2214	4	-0.10290300447942258
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2214	74-75	0.09220137245175408
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2214	78-79	3.02179821660701
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2214	84-85	1.7342973034216342
2214	86-87	1.8514226883600244
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2214	90-91	0.14216497181254084
2214	92-93	0.07416596788501195
2214	94-95	0.13610526516500698
2214	96-97	0.04713460832333638
2214	98-99	0.032599269609125514
2214	100	0.08767444404973546
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2215	3	-0.19734103073652776
2215	4	-0.1888228125675937
2215	5	-0.15378957543870087
2215	6	-0.3414403731243141
2215	7	3.5923863210072007
2215	8	1.7751496747714626
2215	9	0.452819633492318
2215	10-11	-0.190398200323024
2215	12-13	-0.4611861483756172
2215	14-15	-0.536497985738869
2215	16-17	-0.5836086505866476
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2215	20-21	-0.5816433228569196
2215	22-23	-0.5752732043571314
2215	24-25	-0.5027668407830959
2215	26-27	-0.5967919920084057
2215	28-29	-0.4180345818501152
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2215	86-87	1.9609454200301286
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2215	100	-0.20064690770590943
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2216	2	-0.01901389811595422
2216	3	-0.1240614132320843
2216	4	-0.16581367442793038
2216	5	0.006116177232385667
2216	6	-0.24783688886427768
2216	7	3.8428186011344074
2216	8	1.9697414496336805
2216	9	0.7380122344263285
2216	10-11	-0.007661945226885791
2216	12-13	-0.2698874553791839
2216	14-15	-0.4637381635098805
2216	16-17	-0.5522106546750436
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2216	20-21	-0.5190842735095487
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2216	36-37	-0.42486632776394373
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2216	40-41	-0.5341447400096442
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2216	58-59	-0.6763815075747885
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2216	62-63	-0.6347988768269488
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2216	66-67	3.0213825896275566
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2216	72-73	0.027191028041876564
2216	74-75	-0.6821119109330667
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2216	80-81	0.6817321291857859
2216	82-83	-0.18318735982242274
2216	84-85	1.6711861208383745
2216	86-87	1.5983194899272775
2216	88-89	0.35765799102993867
2216	90-91	0.05947419164995793
2216	92-93	-0.001800174660306908
2216	94-95	0.05800672518473249
2216	96-97	-0.008985772508733447
2216	98-99	-0.007208744135802192
2216	100	-1.1251208997100228
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82582.0
3	14600.0
4	9046.0
5	8319.0
6	8036.0
7	9749.0
8	11657.0
9	13502.0
10	15292.0
11	16982.0
12	18990.0
13	21583.0
14	24619.0
15	25745.0
16	26618.0
17	26820.0
18	25626.0
19	24500.0
20	24201.0
21	24223.0
22	26113.0
23	29551.0
24	35502.0
25	43979.0
26	55273.0
27	68397.0
28	86414.0
29	110587.0
30	137168.0
31	171031.0
32	228046.0
33	320942.0
34	482373.0
35	652096.0
36	735135.0
37	1214612.0
38	1343005.0
39	435692.0
40	923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67490624734238	20.788439346244886	14.49910461710086	23.03754978931188
2	15.6955168915622	20.409207238516863	40.23581690525489	23.659458964666047
3	18.435910982971002	25.011513848693806	30.330352139185337	26.222223029149855
4	12.774456322335586	17.332399307953768	38.72502509688874	31.168119272821905
5	13.212597155232164	37.464794443426534	35.423352538817085	13.899255862524221
6	30.67440163067292	37.947052151999245	17.886927745405863	13.49161847192197
7	26.952060768975823	32.35458531507815	22.550710789043187	18.142643126902843
8	24.467855425099124	36.29405363075039	20.909629112755233	18.32846183139525
9	24.385807218638423	17.439623912687274	22.052115967718727	36.12245290095558
10-11	23.489404464372573	27.211243040162163	29.107444721098886	20.191907774366374
12-13	24.05610142568404	24.709688088213245	28.966073074193332	22.26813741190938
14-15	21.60827193586714	27.185552858607625	26.124751097998058	25.081424107527177
16-17	21.06268168410857	30.377601864705795	26.829671074932964	21.730045376252676
18-19	20.934335865687252	29.35808285280237	28.759553063463372	20.948028218047003
20-21	22.163228272392782	28.69133337640246	28.18548038748298	20.959957963721774
22-23	21.81187948490732	28.583685766413915	28.20316697301729	21.401267775661474
24-25	21.676113381150156	28.623045605821535	28.201434625674537	21.499406387353773
26-27	21.381513227774995	28.566005658050386	28.339272115125446	21.713208999049176
28-29	21.391070377329456	28.64881900056721	28.42038366122609	21.539726960877243
30-31	21.783972806534322	29.05138172477948	27.902457194756238	21.262188273929958
32-33	21.265172036490352	28.804711924966504	28.411529537358792	21.518586501184352
34-35	21.45637977670411	28.43034432423747	28.39889728854989	21.71437861050853
36-37	21.889452620519954	28.766789398577323	27.869145875469474	21.47461210543325
38-39	21.672621706118804	28.836469109443215	28.0551198209615	21.435789363476484
40-41	21.488217995563677	28.55106619548836	28.24785245665765	21.712863352290306
42-43	21.435825566074797	28.486275533875073	28.2617587623686	21.81614013768153
44-45	21.54699154356013	28.61858026279976	28.152373871684155	21.682054321955953
46-47	21.471041817365634	28.79876764870575	28.04508494542459	21.685105588504026
48-49	21.311862706606178	28.532187406148275	28.237497721081567	21.91845216616398
50-51	21.349419245904762	28.39023618997475	28.220473227213517	22.039871336906973
52-53	21.308780337430335	28.37755432038293	28.236015893759188	22.07764944842755
54-55	21.4070515448819	28.68598874771503	28.05979179671933	21.84716791068374
56-57	21.236940905538475	28.46100187361188	28.107431106163293	22.19462611468635
58-59	21.133087878744323	28.051783000391104	28.403390269014096	22.41173885185048
60-61	21.414163467076868	28.384276370724848	28.0216738450347	22.179886317163586
62-63	21.24093662588487	28.375520331953346	28.052395370960514	22.331147671201272
64-65	21.255015446428914	28.100434581875227	28.20241724867998	22.442132723015877
66-67	21.162650843473134	28.143335389872792	27.99286355629403	22.70115021036004
68-69	21.739575573639026	28.204886828335667	27.81529914336977	22.24023845465553
70-71	21.48244335617835	27.953204473784844	27.97157983884298	22.592772331193828
72-73	21.281332828703217	28.003393317292186	28.194407365864826	22.520866488139767
74-75	21.32970242119564	27.996937978171406	28.07596228892713	22.59739731170582
76-77	21.34616080038117	28.08076256693182	27.8306245386132	22.74245209407381
78-79	21.59186821182061	28.034587073389478	27.927717082670167	22.445827632119748
80-81	21.383111525079553	27.883732364259846	28.02579192835683	22.70736418230377
82-83	21.33815245099582	27.79909223946141	28.133314127874463	22.729441181668307
84-85	21.441358712417458	27.685207066928374	27.979007526090534	22.894426694563634
86-87	21.58687081127457	27.76446705791635	27.91501928068552	22.733642850123566
88-89	21.50622174637074	27.581700018844263	27.98790699088886	22.924171243896136
90-91	21.589348883862833	27.435261835323686	27.937014242959822	23.038375037853655
92-93	21.76355091374331	27.532652494304994	27.747055056998505	22.956741534953192
94-95	21.707604179590454	27.25325528122664	27.892646075354882	23.146494463828024
96-97	21.77377252792286	27.269242527092967	27.85760816289045	23.099376782093724
98-99	21.9118124113355	27.203532236354743	27.760870328318898	23.12378502399086
100	22.114623001856355	27.167324563029364	27.56870463991314	23.14934779520114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2392.0
1	1852.0
2	1508.5
3	1730.0
4	1640.5
5	1447.0
6	1347.0
7	1322.5
8	1385.5
9	1442.0
10	1294.5
11	1117.0
12	1116.0
13	1206.0
14	1268.5
15	1383.0
16	1679.5
17	2176.0
18	2871.5
19	3911.0
20	5533.0
21	7993.0
22	11648.0
23	16973.0
24	24083.5
25	33208.0
26	45317.5
27	61072.5
28	80374.0
29	101087.0
30	120181.5
31	138715.5
32	156932.5
33	173369.0
34	188815.0
35	202542.0
36	213090.5
37	222272.0
38	229954.0
39	234081.5
40	237725.5
41	239442.0
42	236023.0
43	232574.0
44	229974.0
45	225821.5
46	220940.0
47	214670.0
48	208894.5
49	205567.5
50	200227.0
51	192207.5
52	183603.0
53	175301.5
54	168446.0
55	160343.5
56	147510.0
57	132230.0
58	118313.0
59	104719.5
60	89743.0
61	74774.5
62	62038.0
63	51032.0
64	41658.0
65	33559.5
66	26596.0
67	21001.5
68	16484.0
69	12975.0
70	10245.0
71	8064.0
72	6272.0
73	4920.0
74	3797.0
75	2766.5
76	2032.5
77	1600.0
78	1378.5
79	1338.0
80	934.5
81	438.5
82	273.0
83	175.5
84	142.5
85	112.0
86	73.5
87	52.0
88	49.0
89	40.0
90	31.0
91	31.5
92	22.0
93	16.0
94	18.0
95	14.0
96	15.0
97	14.0
98	15.5
99	27.5
100	53.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.017898400929930107
2	0.0024812660629827026
3	0.001467578098227574
4	0.002178672640667739
5	0.001997116587278761
6	0.003525213369969328
7	11.71313417340328
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	2.1938023117834874E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.2860220448385957E-4
28-29	0.0
30-31	0.0
32-33	1.5129671115748186E-5
34-35	4.538901334724456E-5
36-37	1.5129671115748186E-5
38-39	1.5129671115748186E-5
40-41	0.0
42-43	7.564835557874093E-6
44-45	1.5129671115748187E-4
46-47	7.564835557874093E-4
48-49	5.144088179354383E-4
50-51	2.874637511992156E-4
52-53	2.496395734098451E-4
54-55	4.0850112012520104E-4
56-57	0.001550791289364189
58-59	3.631121067779565E-4
60-61	0.0013314110581858405
62-63	0.002519090240772073
64-65	0.0010969011558917437
66-67	5.856725948248355
68-69	5.496760813062474
70-71	0.0020652001072996276
72-73	0.0010212528003130026
74-75	0.002579608925235066
76-77	0.005719015681752815
78-79	5.4873274631218045
80-81	0.004985226632639028
82-83	0.004493512321377211
84-85	5.856824291110607
86-87	0.002942721032013022
88-89	0.0013692352359752108
90-91	0.002927591360897274
92-93	0.003525213369969328
94-95	0.004069881530136262
96-97	0.0030788880720547564
98-99	0.005219736534933125
100	0.005023050810428398
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6609529.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.9465949811402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.33119172078871	82.12545114114998
2	5.657309688539234	10.063968670987594
3	1.1048673270958147	2.9482255995325914
4	0.3952451554478615	1.4062284303951493
5	0.2003653732840248	0.891090885286956
6	0.10287274932022056	0.5490108461029213
7	0.06371684906299718	0.3967177736956597
8	0.03681814964766713	0.2619879235732846
9	0.02357788335283723	0.18874551969876427
>10	0.0829328864013297	1.094274602865121
>50	0.001022970524416283	0.05921490849732094
>100	6.158193642237517E-5	0.006803443154567091
>500	1.7664598561549188E-5	0.008280255060107542
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0259342231496373E-5	0.0
5	0.0	0.0	0.0	4.538901334724456E-5	0.0
6	0.0	0.0	0.0	4.538901334724456E-5	0.0
7	0.0	0.0	0.0	7.564835557874094E-5	0.0
8	0.0	0.0	0.0	7.564835557874094E-5	0.0
9	4.538901334724456E-5	0.0	0.0	9.077802669448913E-5	0.0
10-11	4.538901334724456E-5	0.0	7.564835557874093E-6	9.834286225236322E-5	0.0
12-13	4.538901334724456E-5	0.0	2.2694506673622278E-5	1.286022044838596E-4	0.0
14-15	4.538901334724456E-5	0.0	4.538901334724456E-5	1.5129671115748187E-4	0.0
16-17	6.0518684462992746E-5	1.5129671115748186E-5	4.538901334724456E-5	1.6642638227323006E-4	0.0
18-19	6.0518684462992746E-5	3.0259342231496373E-5	6.0518684462992746E-5	1.7399121783110414E-4	0.0
20-21	6.0518684462992746E-5	3.0259342231496373E-5	6.0518684462992746E-5	2.4207473785197098E-4	0.0
22-23	6.808352002086684E-5	3.0259342231496373E-5	6.0518684462992746E-5	5.144088179354384E-4	0.0
24-25	7.564835557874094E-5	3.0259342231496373E-5	7.564835557874094E-5	0.0010817714847759954	0.0
26-27	1.059076978102373E-4	3.0259342231496373E-5	7.564835557874094E-5	0.0026401276096980585	0.0
28-29	1.4373187559960776E-4	3.0259342231496373E-5	7.564835557874094E-5	0.007776650953494568	0.0
30-31	1.5129671115748187E-4	3.0259342231496373E-5	7.564835557874094E-5	0.02529681010553097	0.0
32-33	1.5129671115748187E-4	3.0259342231496373E-5	7.564835557874094E-5	0.06747076834067904	0.0
34-35	1.5129671115748187E-4	3.0259342231496373E-5	7.564835557874094E-5	0.13799773024673923	0.0
36-37	1.5129671115748187E-4	3.782417778937047E-5	7.564835557874094E-5	0.25116767019253566	0.0
38-39	1.6642638227323006E-4	4.538901334724456E-5	7.564835557874094E-5	0.4104982367124798	0.0
40-41	1.6642638227323006E-4	4.538901334724456E-5	7.564835557874094E-5	0.5841036479301325	0.0
42-43	1.6642638227323006E-4	4.538901334724456E-5	7.564835557874094E-5	0.7664388793815716	0.0
44-45	1.8155605338897825E-4	4.538901334724456E-5	7.564835557874094E-5	0.9949952560916218	0.0
46-47	1.9668572450472644E-4	4.538901334724456E-5	9.077802669448913E-5	1.2735325013325458	0.0
48-49	2.1938023117834874E-4	4.538901334724456E-5	9.077802669448913E-5	1.5712541695482387	0.0
50-51	2.2694506673622282E-4	4.538901334724456E-5	9.077802669448913E-5	1.879687644913881	0.0
52-53	2.4207473785197098E-4	4.538901334724456E-5	9.077802669448913E-5	2.1812825089352055	0.0
54-55	2.4207473785197098E-4	4.538901334724456E-5	9.077802669448913E-5	2.5125769173567436	0.0
56-57	2.4207473785197098E-4	4.538901334724456E-5	9.077802669448913E-5	2.8730715910316755	0.0
58-59	2.4963957340984506E-4	4.538901334724456E-5	9.077802669448913E-5	3.2597103363946207	7.564835557874093E-6
60-61	2.572044089677192E-4	4.538901334724456E-5	9.077802669448913E-5	3.652794321652874	1.5129671115748186E-5
62-63	2.572044089677192E-4	4.538901334724456E-5	9.077802669448913E-5	4.0417932957098754	1.5129671115748186E-5
64-65	2.572044089677192E-4	4.538901334724456E-5	9.077802669448913E-5	4.462156077989824	1.5129671115748186E-5
66-67	2.572044089677192E-4	4.538901334724456E-5	9.077802669448913E-5	4.918716598414198	1.5129671115748186E-5
68-69	2.572044089677192E-4	4.538901334724456E-5	9.077802669448913E-5	5.366902845876007	1.5129671115748186E-5
70-71	2.647692445255933E-4	4.538901334724456E-5	9.077802669448913E-5	5.887469439955556	1.5129671115748186E-5
72-73	2.7233408008346736E-4	4.538901334724456E-5	9.077802669448913E-5	6.435314831056797	1.5129671115748186E-5
74-75	2.874637511992156E-4	4.538901334724456E-5	9.077802669448913E-5	6.920440170547705	1.5129671115748186E-5
76-77	2.9502858675708966E-4	4.538901334724456E-5	9.077802669448913E-5	7.416806855677613	1.5129671115748186E-5
78-79	3.0259342231496374E-4	4.538901334724456E-5	9.077802669448913E-5	7.977308216667178	1.5129671115748186E-5
80-81	3.0259342231496374E-4	4.538901334724456E-5	9.077802669448913E-5	8.633466923286061	1.5129671115748186E-5
82-83	3.0259342231496374E-4	6.0518684462992746E-5	9.077802669448913E-5	9.294051058706302	1.5129671115748186E-5
84-85	3.0259342231496374E-4	6.0518684462992746E-5	9.077802669448913E-5	9.930631970901405	1.5129671115748186E-5
86-87	3.0259342231496374E-4	6.808352002086684E-5	9.077802669448913E-5	10.65681836027953	1.5129671115748186E-5
88	3.0259342231496374E-4	9.077802669448913E-5	9.077802669448913E-5	11.244931371055335	3.0259342231496373E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6295	0.0	43.298363	1
GTATCAA	9465	0.0	34.697086	1
TCAACGC	9905	0.0	31.958973	4
ATCAACG	9945	0.0	31.778923	3
CAACGCA	10905	0.0	28.981081	5
TATCAAC	11560	0.0	27.915249	2
AACGCAG	11665	0.0	27.224167	6
ACGCAGA	13530	0.0	23.130623	7
GTACATG	16250	0.0	22.448235	1
CGCAGAG	13970	0.0	22.399433	8
TACATGG	16395	0.0	21.27622	2
ACATGGG	16655	0.0	20.113697	3
GCAGAGT	16295	0.0	18.537428	9
GTGGTAT	3840	0.0	17.211315	1
GAGTACA	12660	0.0	16.87555	1
CATGGGG	11855	0.0	16.678022	4
AGAGTAC	14285	0.0	16.381767	10-11
CAGAGTA	16085	0.0	16.26346	10-11
AATACCG	570	0.0	16.175314	5
TACGACC	575	7.2759576E-12	15.143975	4
>>END_MODULE