FastQCFastQC Report
Wed 25 May 2016
SRR2049487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6609529
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191270.28938521943091555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191070.28908262600860063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112050.16952796485195845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121500.056.7322921
GTATCAA218300.040.979381
GTGGTAT50300.033.5104751
ATCAACG275550.032.1411173
TCAACGC279900.031.6920434
TATCAAC282650.031.3504032
CAACGCA289300.030.6885455
TGGTATC53800.030.0022372
AACGCAG302600.029.4166986
ACGCAGA343000.025.8825827
CGCAGAG346000.025.5495018
GCAGAGT372500.023.492179
GTACATG163500.022.9800361
TACATGG166400.021.7760542
GAGTACT280450.021.72635312-13
CAGAGTA367850.020.83146510-11
ACATGGG168350.020.7131843
AGAGTAC349200.020.6789310-11
AGTACTT294150.019.66794412-13
GTACTTT311800.019.2931714-15
Produced by FastQC (version 0.11.5)