##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049487_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6609529 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.643454775673124 31.0 31.0 34.0 30.0 34.0 2 31.915612595088092 33.0 31.0 34.0 30.0 34.0 3 31.895263792624256 33.0 31.0 34.0 30.0 34.0 4 35.56890846533845 37.0 35.0 37.0 33.0 37.0 5 35.70825561095201 37.0 35.0 37.0 33.0 37.0 6 35.789777758748016 37.0 35.0 37.0 35.0 37.0 7 35.77511029908485 37.0 35.0 37.0 33.0 37.0 8 35.76093213298557 37.0 35.0 37.0 33.0 37.0 9 37.49179147258451 39.0 37.0 39.0 35.0 39.0 10-11 37.44889386217989 39.0 37.0 39.0 34.0 39.0 12-13 37.43530219778141 39.0 37.0 39.0 34.0 39.0 14-15 38.69445122337764 40.0 38.0 41.0 34.0 41.0 16-17 38.6979280974484 40.0 38.0 41.0 34.0 41.0 18-19 38.773399738468505 40.0 38.0 41.0 34.5 41.0 20-21 38.801576632767635 40.0 38.0 41.0 35.0 41.0 22-23 38.77457448178229 40.0 38.0 41.0 34.0 41.0 24-25 38.7384626801698 40.0 38.0 41.0 34.0 41.0 26-27 38.639602988352124 40.0 38.0 41.0 34.0 41.0 28-29 38.54874394226881 40.0 38.0 41.0 34.0 41.0 30-31 38.42430708753982 40.0 38.0 41.0 34.0 41.0 32-33 38.268784583591355 40.0 38.0 41.0 34.0 41.0 34-35 38.15690217865751 40.0 38.0 41.0 34.0 41.0 36-37 38.004813580513826 40.0 37.5 41.0 33.0 41.0 38-39 37.899769862572654 40.0 37.0 41.0 33.0 41.0 40-41 37.76401593820074 40.0 37.0 41.0 33.0 41.0 42-43 37.613533354645995 40.0 37.0 41.0 33.0 41.0 44-45 37.420967439586086 40.0 37.0 41.0 32.5 41.0 46-47 37.17061374569958 40.0 37.0 41.0 31.5 41.0 48-49 36.93341272880413 40.0 37.0 41.0 31.0 41.0 50-51 36.71969719778822 40.0 36.0 41.0 31.0 41.0 52-53 36.615908637362814 40.0 36.0 41.0 31.0 41.0 54-55 36.48875751963567 39.5 36.0 41.0 30.5 41.0 56-57 36.31933357127262 39.0 35.0 41.0 30.0 41.0 58-59 36.15454656451314 39.0 35.0 41.0 30.0 41.0 60-61 35.96986116559894 39.0 35.0 41.0 30.0 41.0 62-63 35.77280332683312 39.0 35.0 41.0 29.0 41.0 64-65 35.506211562124925 38.0 35.0 40.0 29.0 41.0 66-67 35.251170015291564 37.5 35.0 40.0 29.0 41.0 68-69 34.94116297848152 37.0 34.5 40.0 28.5 41.0 70-71 34.64729884686186 36.5 34.0 39.5 28.0 41.0 72-73 34.3334879081399 36.0 34.0 39.0 28.0 41.0 74-75 33.98906253380535 36.0 34.0 39.0 28.0 40.5 76-77 33.6975593873633 35.0 34.0 38.5 28.0 40.0 78-79 33.36599029976266 35.0 34.0 37.0 27.0 39.0 80-81 33.05292109316716 35.0 34.0 37.0 27.0 39.0 82-83 32.75485356066976 35.0 33.0 36.0 26.0 39.0 84-85 32.47795311889848 35.0 33.0 36.0 26.0 37.0 86-87 32.20764906243697 35.0 33.0 36.0 26.0 37.0 88-89 31.988570365603962 35.0 33.0 35.0 26.0 37.0 90-91 31.830302053293057 35.0 33.0 35.0 26.0 36.0 92-93 31.665048220531297 35.0 33.0 35.0 25.0 36.0 94-95 31.47849309686061 35.0 33.0 35.0 25.0 36.0 96-97 31.30188497546497 35.0 33.0 35.0 24.5 35.5 98-99 31.113358304351188 35.0 33.0 35.0 24.0 35.0 100 30.99492611349462 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4407472440492093 1101 2 0.5012761184995149 1101 3 0.5514001177751311 1101 4 0.45473664889541965 1101 5 0.33114138166487805 1101 6 0.3573969347231838 1101 7 0.3597655466148737 1101 8 0.3642576785439715 1101 9 0.40174271612610113 1101 10-11 0.4289441277944803 1101 12-13 0.35094362321379435 1101 14-15 0.5496580737832844 1101 16-17 0.5571770540961793 1101 18-19 0.5597963749506931 1101 20-21 0.5048262648612507 1101 22-23 0.5648996412187159 1101 24-25 0.5917372833719696 1101 26-27 0.6525848815205464 1101 28-29 0.6117476587056174 1101 30-31 0.6024180788920859 1101 32-33 0.5964622767971051 1101 34-35 0.6699141684806023 1101 36-37 0.649115864135851 1101 38-39 0.6744614282510781 1101 40-41 0.7313299990674409 1101 42-43 0.6243037343574542 1101 44-45 0.6309928694748734 1101 46-47 0.7581936700394394 1101 48-49 0.75117118761721 1101 50-51 0.8221843827990511 1101 52-53 0.8084681194374497 1101 54-55 0.7097106724781028 1101 56-57 0.7328688061665787 1101 58-59 0.7555129195005534 1101 60-61 0.7885342555983286 1101 62-63 0.7596511966307062 1101 64-65 0.7476255472997622 1101 66-67 0.7413746570341715 1101 68-69 0.7015244004262442 1101 70-71 0.6977878611270185 1101 72-73 0.7115768268972573 1101 74-75 0.7417783633272705 1101 76-77 0.7244116713870881 1101 78-79 0.6860681872548255 1101 80-81 0.6866090222740908 1101 82-83 0.7041939689168899 1101 84-85 0.7065625256944728 1101 86-87 0.7594570876752655 1101 88-89 0.7453298119895067 1101 90-91 0.7541783007210228 1101 92-93 0.7689377357273486 1101 94-95 0.6885505424024423 1101 96-97 0.7371673228814295 1101 98-99 0.7773437691244922 1101 100 0.8629904726000035 1102 1 0.3634889805062791 1102 2 0.4047314761720777 1102 3 0.4396544118168393 1102 4 0.4254449874807307 1102 5 0.36261621916020914 1102 6 0.32555079612838966 1102 7 0.3125597257316528 1102 8 0.3130589366917462 1102 9 0.41796096106306635 1102 10-11 0.38312038776805224 1102 12-13 0.3427238317167465 1102 14-15 0.42063480180924984 1102 16-17 0.469114170871066 1102 18-19 0.4739123230931028 1102 20-21 0.4173163266821831 1102 22-23 0.41138914908026436 1102 24-25 0.4310393396395966 1102 26-27 0.4170497050550992 1102 28-29 0.4601206081588032 1102 30-31 0.48268298647626295 1102 32-33 0.4904724310059052 1102 34-35 0.49348798568627217 1102 36-37 0.4784826378075593 1102 38-39 0.502603911354143 1102 40-41 0.4930760408816397 1102 42-43 0.48282085638161476 1102 44-45 0.5255353383863408 1102 46-47 0.5402578106729905 1102 48-49 0.5417800772406238 1102 50-51 0.5896513692906993 1102 52-53 0.6073986899975736 1102 54-55 0.5613482596586366 1102 56-57 0.5751159467930975 1102 58-59 0.5758329410386054 1102 60-61 0.5574877256705477 1102 62-63 0.5856183252637877 1102 64-65 0.589843430143489 1102 66-67 0.602538785667079 1102 68-69 0.5379776209733009 1102 70-71 0.5838298959954358 1102 72-73 0.5634631165018789 1102 74-75 0.5430487844932799 1102 76-77 0.5168697746027817 1102 78-79 0.46866324506982693 1102 80-81 0.46858972300204016 1102 82-83 0.4425688183227052 1102 84-85 0.5312751987143614 1102 86-87 0.5655684603332176 1102 88-89 0.5439128505811084 1102 90-91 0.49017900953476357 1102 92-93 0.5788082995667523 1102 94-95 0.5962599602798555 1102 96-97 0.5921518719319927 1102 98-99 0.6745511081214843 1102 100 0.7584717432912065 1103 1 0.3502224681809132 1103 2 0.3360095924855422 1103 3 0.4834612735523294 1103 4 0.36953543199916083 1103 5 0.2500767627616227 1103 6 0.29753349467505075 1103 7 0.2759236369673346 1103 8 0.30659444293379323 1103 9 0.3464105730160014 1103 10-11 0.31752188291714134 1103 12-13 0.2894425950248092 1103 14-15 0.42640482423777115 1103 16-17 0.45378437603143595 1103 18-19 0.4354711282901178 1103 20-21 0.4138313878637945 1103 22-23 0.3913872391711948 1103 24-25 0.4228294406324906 1103 26-27 0.4511010448305015 1103 28-29 0.4576377586260847 1103 30-31 0.4668932164912576 1103 32-33 0.45457152818672597 1103 34-35 0.459789237063859 1103 36-37 0.4012482055022204 1103 38-39 0.4035974028227898 1103 40-41 0.416777980213638 1103 42-43 0.44737570238874724 1103 44-45 0.5484135165137545 1103 46-47 0.5255832565980398 1103 48-49 0.5264587673011007 1103 50-51 0.5421723202189384 1103 52-53 0.4929083034216504 1103 54-55 0.467168723689106 1103 56-57 0.5424848599257857 1103 58-59 0.48551785766074573 1103 60-61 0.45320425451404844 1103 62-63 0.4404906097731356 1103 64-65 0.46293110142232763 1103 66-67 0.4779515615392427 1103 68-69 0.469122721153191 1103 70-71 0.5008834644282558 1103 72-73 0.5001141909547826 1103 74-75 0.44991990821667116 1103 76-77 0.43925684477645177 1103 78-79 0.3472035615727336 1103 80-81 0.3280862780680778 1103 82-83 0.3545315757410279 1103 84-85 0.3994250291454051 1103 86-87 0.4602547351846198 1103 88-89 0.4853883488996189 1103 90-91 0.47271645601214374 1103 92-93 0.4842367321766403 1103 94-95 0.4774186266213256 1103 96-97 0.4590388717745526 1103 98-99 0.5180260425006189 1103 100 0.5057362179500657 1104 1 0.35350090146148716 1104 2 0.3179100384552136 1104 3 0.40426541849653574 1104 4 0.3014610542568761 1104 5 0.24769034085396413 1104 6 0.2657678727960402 1104 7 0.2190134739692624 1104 8 0.2653344018758048 1104 9 0.29851289340224696 1104 10-11 0.3269145061258172 1104 12-13 0.31280855205254454 1104 14-15 0.3811545036041295 1104 16-17 0.3487780096670008 1104 18-19 0.40848539423589614 1104 20-21 0.38116977209666203 1104 22-23 0.37945872624545984 1104 24-25 0.37013104474227987 1104 26-27 0.39585943736387463 1104 28-29 0.3794678746119544 1104 30-31 0.42843218385901594 1104 32-33 0.4036735842039434 1104 34-35 0.35935384112723767 1104 36-37 0.393162835194687 1104 38-39 0.42353141964629515 1104 40-41 0.4071486957810606 1104 42-43 0.4154738465881067 1104 44-45 0.4078421778833601 1104 46-47 0.4526047252364478 1104 48-49 0.38248226496411775 1104 50-51 0.4765227797054905 1104 52-53 0.48796732954541255 1104 54-55 0.43556023107317543 1104 56-57 0.40820656937773947 1104 58-59 0.4813730472330988 1104 60-61 0.4803596468036275 1104 62-63 0.47625596841509577 1104 64-65 0.47813232083349533 1104 66-67 0.42875146052536905 1104 68-69 0.3830676062298508 1104 70-71 0.422206509494373 1104 72-73 0.4233304023232307 1104 74-75 0.4547367449326245 1104 76-77 0.40637492491185867 1104 78-79 0.39693697488567636 1104 80-81 0.36824848272264177 1104 82-83 0.40319395487504295 1104 84-85 0.42802245726383603 1104 86-87 0.43102627392867277 1104 88-89 0.41261121240626153 1104 90-91 0.4220935474114853 1104 92-93 0.44829588559681355 1104 94-95 0.4267013313810075 1104 96-97 0.47287067708780484 1104 98-99 0.5084522663033617 1104 100 0.5537877132095872 1105 1 0.31757823593167345 1105 2 0.3067535806481949 1105 3 0.39806865707044636 1105 4 0.2923417412284408 1105 5 0.2235750287179883 1105 6 0.2482518407838441 1105 7 0.23166118069310215 1105 8 0.22415572584254306 1105 9 0.29328015033222954 1105 10-11 0.25247083677967197 1105 12-13 0.23182182562953102 1105 14-15 0.34821310143038176 1105 16-17 0.3236019257601299 1105 18-19 0.32365756613269525 1105 20-21 0.2969339204343271 1105 22-23 0.3152161673207843 1105 24-25 0.35543614953027003 1105 26-27 0.33799974970526847 1105 28-29 0.41584131209911135 1105 30-31 0.3968841513437127 1105 32-33 0.3876995134475578 1105 34-35 0.36875763704506426 1105 36-37 0.43777898811852367 1105 38-39 0.4021785128051505 1105 40-41 0.462564651125291 1105 42-43 0.4648987834776577 1105 44-45 0.4825223138789383 1105 46-47 0.4203380885080108 1105 48-49 0.46470792296239694 1105 50-51 0.507686603294573 1105 52-53 0.5146386225731803 1105 54-55 0.4463714301905739 1105 56-57 0.48069561630257596 1105 58-59 0.4546241598825631 1105 60-61 0.46167364607171635 1105 62-63 0.4041888659271464 1105 64-65 0.4583780723344617 1105 66-67 0.43741217007068656 1105 68-69 0.46591152022772775 1105 70-71 0.4149682045201928 1105 72-73 0.43463466166195985 1105 74-75 0.4481767508233503 1105 76-77 0.40059596007800025 1105 78-79 0.3980523204414297 1105 80-81 0.4183222017733996 1105 82-83 0.4113847037435079 1105 84-85 0.4087839636219712 1105 86-87 0.45894017233698037 1105 88-89 0.3888780155225362 1105 90-91 0.38599360102534064 1105 92-93 0.4199271760205221 1105 94-95 0.4436658580653159 1105 96-97 0.5399701232833856 1105 98-99 0.5864483473407134 1105 100 0.6014669506635038 1106 1 0.2065466092409345 1106 2 0.21797798346228703 1106 3 0.34950238157751556 1106 4 0.2699182936174296 1106 5 0.20684839611548256 1106 6 0.19205087037207846 1106 7 0.16375029495933546 1106 8 0.2147881308741617 1106 9 0.21473269557804286 1106 10-11 0.2374935854047635 1106 12-13 0.18002171303625403 1106 14-15 0.27301147913053825 1106 16-17 0.2423372230161931 1106 18-19 0.2236326067300496 1106 20-21 0.2049255593410777 1106 22-23 0.25556572467466765 1106 24-25 0.27101227325532307 1106 26-27 0.2974677035493798 1106 28-29 0.298262441613268 1106 30-31 0.28639080759325 1106 32-33 0.31065046607497493 1106 34-35 0.27120546760076536 1106 36-37 0.2697500818186995 1106 38-39 0.2295269043648176 1106 40-41 0.23572860189823075 1106 42-43 0.27246723172645915 1106 44-45 0.28759896132216767 1106 46-47 0.2509154111429339 1106 48-49 0.29717414191339486 1106 50-51 0.3238491043371141 1106 52-53 0.3271533406484437 1106 54-55 0.23591414572452862 1106 56-57 0.3171197709944593 1106 58-59 0.29143987670188665 1106 60-61 0.30326698810506514 1106 62-63 0.30036250364663175 1106 64-65 0.2752683515363543 1106 66-67 0.30497210636595895 1106 68-69 0.3430292366202252 1106 70-71 0.3625174303494276 1106 72-73 0.29923649479823666 1106 74-75 0.29581094287460985 1106 76-77 0.31108514903846185 1106 78-79 0.34463590255111853 1106 80-81 0.25544837799291287 1106 82-83 0.29259021222847537 1106 84-85 0.2804704036139256 1106 86-87 0.34244622330567864 1106 88-89 0.37220013654436457 1106 90-91 0.368213476191535 1106 92-93 0.37759185901771275 1106 94-95 0.33275151603686837 1106 96-97 0.369175598652685 1106 98-99 0.41820978512495444 1106 100 0.40421959869054547 1107 1 0.2074477271694697 1107 2 0.22791511772276962 1107 3 0.32420347799306626 1107 4 0.23502760276657142 1107 5 0.21033439698496892 1107 6 0.20362192748159913 1107 7 0.14527958101189142 1107 8 0.2048330691214204 1107 9 0.1570732492088851 1107 10-11 0.15229014384912887 1107 12-13 0.1457329937613565 1107 14-15 0.17270320096158542 1107 16-17 0.1859180509824796 1107 18-19 0.19521929623932976 1107 20-21 0.2330998552761656 1107 22-23 0.19082330352202348 1107 24-25 0.17944080904232607 1107 26-27 0.19563442138409926 1107 28-29 0.21418940314236323 1107 30-31 0.2551361203010032 1107 32-33 0.2067223284276949 1107 34-35 0.21860350685599883 1107 36-37 0.2875259933943326 1107 38-39 0.24436166143982518 1107 40-41 0.2147925122876586 1107 42-43 0.19234786824207362 1107 44-45 0.2425148384321929 1107 46-47 0.22445642140194622 1107 48-49 0.2085164367519141 1107 50-51 0.34821242625660886 1107 52-53 0.31797761126095025 1107 54-55 0.27483291999200077 1107 56-57 0.2936199099325236 1107 58-59 0.22705157020750022 1107 60-61 0.19396969724793678 1107 62-63 0.17941364069782395 1107 64-65 0.21712398517609444 1107 66-67 0.19420349127967285 1107 68-69 0.2796525542371242 1107 70-71 0.30119925359033317 1107 72-73 0.2700114984210842 1107 74-75 0.2767673895261282 1107 76-77 0.2573129245504049 1107 78-79 0.22252812060022364 1107 80-81 0.2448946687200646 1107 82-83 0.26517449198400556 1107 84-85 0.32956331919186255 1107 86-87 0.3336311259668676 1107 88-89 0.2906719522856065 1107 90-91 0.2822430460960561 1107 92-93 0.2005133063489808 1107 94-95 0.22547016234686978 1107 96-97 0.28402987988723183 1107 98-99 0.42330994191581084 1107 100 0.43437956047510795 1108 1 0.1199130335126668 1108 2 0.172499404547505 1108 3 0.24505128724505454 1108 4 0.15814586038229805 1108 5 0.14616215274525501 1108 6 0.1740077868253067 1108 7 0.11149795472535118 1108 8 0.16417659517117755 1108 9 0.14231259437921295 1108 10-11 0.12232646681243864 1108 12-13 0.1328779605699708 1108 14-15 0.18621780609954186 1108 16-17 0.19415773208667986 1108 18-19 0.19560052945965367 1108 20-21 0.1280576711651662 1108 22-23 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-0.8232825256416945 2215 44-45 -0.921802006504187 2215 46-47 -0.8700284294359975 2215 48-49 -0.8454442570142859 2215 50-51 -0.8572490663491408 2215 52-53 -0.9992059438167118 2215 54-55 -0.8883408276761244 2215 56-57 -0.8369950446502301 2215 58-59 -0.8065672138924072 2215 60-61 -0.9823643938538495 2215 62-63 -0.9582623344503318 2215 64-65 -0.883934962553198 2215 66-67 -0.7856077110682733 2215 68-69 -0.7394526001994635 2215 70-71 -0.8563673641343712 2215 72-73 -0.7466606884130798 2215 74-75 -0.8844174866940193 2215 76-77 -0.7941737173045595 2215 78-79 -0.7832163268112069 2215 80-81 -0.8195085707994707 2215 82-83 -0.8045750297555472 2215 84-85 -0.7859363602950573 2215 86-87 -0.7478192755923061 2215 88-89 -0.7393618395914068 2215 90-91 -0.7292447262046977 2215 92-93 -0.8712456488021658 2215 94-95 -0.8463816716033215 2215 96-97 -0.9627834251426961 2215 98-99 -0.9325328780498694 2215 100 -0.9708513396793244 2216 1 -0.8578731772927561 2216 2 -1.360752059650494 2216 3 -0.9655880086369457 2216 4 -1.3903306570740668 2216 5 -0.7875873667893316 2216 6 -0.9040442272205667 2216 7 -0.7140458669780045 2216 8 -0.6908238282898651 2216 9 -0.7385091806391912 2216 10-11 -0.9935854076867372 2216 12-13 -0.8208532198439826 2216 14-15 -1.1253558728648656 2216 16-17 -1.0744273216928661 2216 18-19 -1.0517503959655983 2216 20-21 -0.8921935389562066 2216 22-23 -0.955209411889868 2216 24-25 -1.0031046817915836 2216 26-27 -1.1338315883243553 2216 28-29 -1.3998835721344918 2216 30-31 -1.525639969237858 2216 32-33 -1.2753350455592312 2216 34-35 -1.3091104815151482 2216 36-37 -1.2593633925040422 2216 38-39 -1.3875671763336754 2216 40-41 -1.2074624711898423 2216 42-43 -1.7584790110261324 2216 44-45 -1.5612964772830153 2216 46-47 -1.4661515458725844 2216 48-49 -1.374498491143278 2216 50-51 -1.5000764236094923 2216 52-53 -1.503074402403179 2216 54-55 -1.6417444673621802 2216 56-57 -1.6498749962094195 2216 58-59 -1.4860122773419633 2216 60-61 -1.7303686274247667 2216 62-63 -1.7367791862644637 2216 64-65 -1.6227726931764437 2216 66-67 -1.540288533856483 2216 68-69 -1.898331808832502 2216 70-71 -1.6467927218588514 2216 72-73 -1.5021781582188822 2216 74-75 -1.5383006459851742 2216 76-77 -1.6581215156912634 2216 78-79 -1.6869715462664558 2216 80-81 -1.526595879643807 2216 82-83 -1.6362135565591203 2216 84-85 -1.6680580061211074 2216 86-87 -1.7749688188533455 2216 88-89 -1.549308260978428 2216 90-91 -1.4518126351794223 2216 92-93 -1.5874076649495592 2216 94-95 -1.7414796571657298 2216 96-97 -1.8484539424958868 2216 98-99 -1.8729295614492756 2216 100 -1.857591486066699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 390.0 3 33.0 4 63.0 5 166.0 6 438.0 7 1171.0 8 2492.0 9 4302.0 10 6131.0 11 8992.0 12 12606.0 13 17653.0 14 21496.0 15 27423.0 16 33630.0 17 46114.0 18 45084.0 19 24493.0 20 18290.0 21 17707.0 22 18688.0 23 20645.0 24 24179.0 25 29497.0 26 37568.0 27 48535.0 28 62933.0 29 82216.0 30 107237.0 31 137811.0 32 176813.0 33 230715.0 34 312362.0 35 451519.0 36 731347.0 37 1324536.0 38 1819236.0 39 702983.0 40 2035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.14317470616473 20.776903796246156 15.126822358472442 22.95309913911667 2 15.748154439867035 20.886764542792953 39.51897500435357 23.846106012986436 3 18.029378492779138 25.254764749500303 30.939647893215994 25.776208864504568 4 12.740328030360637 17.85402136962779 37.99299756759844 31.412653032413136 5 12.635919806482029 37.397381189128474 35.508771430802014 14.457927573587485 6 30.191113447841527 38.43213290112226 17.301626086989412 14.075127564046797 7 26.151439837846237 32.9525598571396 22.458922564678964 18.4370777403352 8 24.70769097162597 35.97065691065127 20.443983224825853 18.877668892896907 9 24.382436617220847 16.77276608724017 21.98778610986207 36.85701118567691 10-11 23.55656507445538 27.107461061143688 28.719693944908936 20.616279919491994 12-13 24.58641152796213 24.62434161344931 28.963009315792398 21.826237542796164 14-15 21.723442930963806 26.70054756578214 26.97363827403344 24.602371229220616 16-17 20.4583412827147 29.911722907940945 27.70082406779666 21.929111741547697 18-19 20.225495644243335 28.03141494651132 30.950851414677206 20.792237994568147 20-21 21.389443937684515 27.284047017571144 30.81755901214746 20.508950032596875 22-23 21.027413602391334 27.21827077239543 30.90798149157073 20.846334133642504 24-25 20.96790104105754 27.242576589042883 30.867797085087307 20.921725284812275 26-27 20.772017189121947 27.15151866343275 30.9492174101967 21.1272467372486 28-29 20.787749713083898 27.21111495321779 31.06836731706531 20.932768016633002 30-31 21.136192670433363 27.57807545013968 30.591773603586592 20.693958275840366 32-33 20.68263265677726 27.373639435853857 31.005479437754147 20.93824846961474 34-35 20.85912627057087 27.05864517728873 30.906612256334753 21.175616295805646 36-37 21.314688232701602 27.37571769486146 30.330663501136012 20.978930571300918 38-39 21.157052189346622 27.420418308172945 30.45910684407316 20.96342265840728 40-41 21.011338326830852 27.20353447272869 30.56139855048673 21.223728649953728 42-43 21.053724100461622 27.212423154509196 30.340823075290235 21.393029669738947 44-45 21.24188425529262 27.39236033308879 30.093732851463393 21.272022560155193 46-47 21.279715997917553 27.636205242461305 29.646219874366235 21.437858885254908 48-49 21.238374171593772 27.51221758766774 29.51404706749906 21.735361173239426 50-51 21.340090950504944 27.522989913502155 29.262743230266487 21.874175905726414 52-53 21.237693336393562 27.579605142817286 29.267607419530194 21.915094101258955 54-55 21.31524046569733 27.91984875170379 29.003481186027024 21.76142959657186 56-57 21.11159509247936 27.62777045081427 29.099161226163012 22.161473230543358 58-59 20.930940663004375 27.256446346549573 29.446764583020336 22.365848407425716 60-61 21.191903386761748 27.55353671948485 29.11742273919972 22.137137154553677 62-63 20.95665213058298 27.52254358820424 29.159831207337167 22.360973073875613 64-65 20.869490095285155 27.284160490104515 29.346690210452213 22.499659204158117 66-67 20.871918407499233 27.35368889371694 29.079734728450397 22.694657970333438 68-69 21.29872642967449 27.47337972191362 28.80695432306901 22.42093952534288 70-71 21.214620587942047 27.22902040372317 28.838598030207596 22.717760978127185 72-73 20.99297847093189 27.309411911196698 28.975899795583015 22.721709822288396 74-75 21.03454724232241 27.29719470177073 28.762874026273277 22.90538402963358 76-77 20.995149578737003 27.377624033422048 28.560408767402336 23.066817620438613 78-79 21.11974209330115 27.346841420605 28.631533414973127 22.901883071120725 80-81 20.95233549118399 27.200031046179074 28.628692388496635 23.218941074140304 82-83 20.769969739798274 27.132192093312867 28.757646278129844 23.34019188875902 84-85 20.780232600537797 26.999548681910618 28.59761262867596 23.62260608887562 86-87 20.836923478208508 27.098670722225442 28.52236520938179 23.542040590184264 88-89 20.705257782081667 26.965017842049654 28.547347529357815 23.782376846510868 90-91 20.649910299942015 26.797233236415156 28.50465483349604 24.048201630146785 92-93 20.67319763391568 26.913509379814816 28.36600519057043 24.047287795699077 94-95 20.51624449682876 26.58848053883779 28.564837393899627 24.330437570433823 96-97 20.45082940100573 26.60071542162838 28.534832058381166 24.413623118984727 98-99 20.482523145565246 26.57449416769958 28.45578598470163 24.487196702033543 100 20.620098694841214 26.515644838976353 28.28565221022461 24.57860425595782 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1040.0 1 779.5 2 1181.5 3 1793.0 4 1128.5 5 646.0 6 1450.5 7 3866.0 8 6357.5 9 7165.5 10 5782.5 11 3888.0 12 3559.5 13 3906.5 14 4528.5 15 5210.0 16 5943.0 17 6800.0 18 7681.5 19 8876.0 20 10474.5 21 12548.5 22 15495.0 23 20038.0 24 26326.5 25 33885.0 26 44397.5 27 59113.0 28 77318.5 29 96952.0 30 115054.5 31 132829.0 32 150522.0 33 166393.5 34 181728.5 35 195125.0 36 205331.0 37 214263.0 38 222650.5 39 228560.5 40 232389.5 41 234893.0 42 233035.0 43 230529.5 44 229036.0 45 226183.5 46 222169.0 47 216057.0 48 209504.0 49 206185.0 50 201481.5 51 193286.0 52 183781.5 53 174813.0 54 168517.0 55 160851.5 56 148823.5 57 134311.5 58 120708.5 59 107930.0 60 93434.5 61 77651.5 62 64412.0 63 53038.0 64 42778.0 65 34207.0 66 27140.0 67 21439.0 68 16690.5 69 12949.0 70 10039.0 71 7767.0 72 5986.0 73 4696.0 74 3548.5 75 2508.0 76 1767.5 77 1341.5 78 1128.0 79 1081.0 80 756.5 81 285.5 82 132.0 83 75.5 84 42.5 85 24.0 86 14.5 87 7.0 88 5.0 89 5.5 90 4.5 91 3.5 92 1.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 1.0 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05858208656017697 2 2.7233408008346736E-4 3 0.0 4 0.11835941713849807 5 0.001770171520542538 6 0.0015734857960378115 7 0.0 8 0.0 9 7.564835557874094E-5 10-11 0.0 12-13 0.0 14-15 5.295384890511866E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.7989891564134144E-4 30-31 3.7824177789370466E-4 32-33 7.564835557874093E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5129671115748187E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.0590769781023732E-4 80-81 3.0259342231496374E-4 82-83 3.404176001043342E-4 84-85 0.0 86-87 0.0 88-89 3.782417778937047E-5 90-91 2.269450667362228E-5 92-93 6.581406935350461E-4 94-95 6.732703646507944E-4 96-97 0.0 98-99 2.269450667362228E-5 100 9.834286225236322E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6609529.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.56468534206939 #Duplication Level Percentage of deduplicated Percentage of total 1 75.77251631292498 45.89138608069121 2 14.489138070509961 17.550601762364682 3 4.521173094784334 8.214702775879298 4 1.867246283660161 4.5235673450410445 5 0.9866168191211248 2.9877068601632155 6 0.5796746551109909 2.1064687852541857 7 0.37072636791933694 1.5717048074730022 8 0.25807846215303415 1.2504352683090942 9 0.19032243853648473 1.0374136743147786 >10 0.9010389619113179 9.431786048644785 >50 0.042835565408107144 1.7631342855436136 >100 0.019209263507330616 2.0660826276174866 >500 9.24182706532454E-4 0.365150644956459 >1k 3.9961739752652304E-4 0.4024606789033528 >5k 2.4976087180276364E-5 0.08954999658005224 >10k+ 7.49282615408291E-5 0.7478483582637167 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19127 0.28938521943091555 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19107 0.28908262600860063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11205 0.16952796485195845 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.0259342231496373E-5 0.0 5 0.0 0.0 0.0 3.0259342231496373E-5 0.0 6 0.0 0.0 0.0 3.0259342231496373E-5 0.0 7 0.0 0.0 0.0 4.538901334724456E-5 0.0 8 0.0 1.5129671115748186E-5 0.0 4.538901334724456E-5 0.0 9 1.5129671115748186E-5 1.5129671115748186E-5 0.0 7.564835557874094E-5 0.0 10-11 2.2694506673622278E-5 1.5129671115748186E-5 0.0 9.834286225236322E-5 0.0 12-13 3.0259342231496373E-5 1.5129671115748186E-5 0.0 1.3616704004173368E-4 0.0 14-15 6.0518684462992746E-5 1.5129671115748186E-5 0.0 1.3616704004173368E-4 0.0 16-17 6.0518684462992746E-5 2.2694506673622278E-5 0.0 1.6642638227323006E-4 0.0 18-19 6.808352002086684E-5 3.0259342231496373E-5 0.0 1.7399121783110414E-4 0.0 20-21 8.321319113661503E-5 3.0259342231496373E-5 0.0 2.4207473785197098E-4 0.0 22-23 9.077802669448913E-5 3.0259342231496373E-5 0.0 4.614549690303197E-4 0.0 24-25 9.077802669448913E-5 3.0259342231496373E-5 0.0 9.909934580815062E-4 0.0 26-27 9.077802669448913E-5 3.0259342231496373E-5 0.0 0.0025720440896771918 0.0 28-29 9.834286225236322E-5 4.538901334724456E-5 0.0 0.007738826775705198 0.0 30-31 1.2103736892598549E-4 4.538901334724456E-5 0.0 0.02533463428332034 0.0 32-33 1.3616704004173368E-4 4.538901334724456E-5 0.0 0.06787926946080425 0.0 34-35 1.5129671115748187E-4 4.538901334724456E-5 0.0 0.13917784459376759 0.0 36-37 1.6642638227323006E-4 4.538901334724456E-5 0.0 0.25358085273549746 0.0 38-39 1.6642638227323006E-4 4.538901334724456E-5 0.0 0.4146210720915212 0.0 40-41 1.6642638227323006E-4 6.0518684462992746E-5 0.0 0.5892704306161605 0.0 42-43 1.6642638227323006E-4 6.0518684462992746E-5 7.564835557874093E-6 0.77242266430785 0.0 44-45 1.6642638227323006E-4 6.0518684462992746E-5 3.0259342231496373E-5 1.001576663026972 0.0 46-47 1.6642638227323006E-4 6.0518684462992746E-5 6.0518684462992746E-5 1.2817706072550705 0.0 48-49 1.7399121783110414E-4 6.0518684462992746E-5 6.0518684462992746E-5 1.5804605744221714 0.0 50-51 1.8155605338897825E-4 6.0518684462992746E-5 6.0518684462992746E-5 1.8903540630504836 0.0 52-53 2.2694506673622282E-4 6.0518684462992746E-5 6.0518684462992746E-5 2.193703968921235 0.0 54-55 2.2694506673622282E-4 6.0518684462992746E-5 6.0518684462992746E-5 2.537654347231096 0.0 56-57 2.345099022940969E-4 6.0518684462992746E-5 6.0518684462992746E-5 2.949657986219593 0.0 58-59 2.4207473785197098E-4 6.0518684462992746E-5 9.077802669448913E-5 3.392896831226552 0.0 60-61 2.572044089677192E-4 6.0518684462992746E-5 9.077802669448913E-5 3.844948709658434 0.0 62-63 3.101582578728378E-4 6.0518684462992746E-5 9.077802669448913E-5 4.29161442517311 0.0 64-65 3.177230934307119E-4 6.0518684462992746E-5 1.0590769781023732E-4 4.771943658920325 0.0 66-67 3.177230934307119E-4 6.0518684462992746E-5 1.0590769781023732E-4 5.313366504632932 0.0 68-69 3.177230934307119E-4 6.0518684462992746E-5 1.0590769781023732E-4 5.904626487000813 0.0 70-71 3.177230934307119E-4 6.0518684462992746E-5 1.0590769781023732E-4 6.5203587123984175 0.0 72-73 3.25287928988586E-4 6.0518684462992746E-5 1.0590769781023732E-4 7.147846692252958 0.0 74-75 3.328527645464601E-4 6.0518684462992746E-5 1.0590769781023732E-4 7.769993898203639 0.0 76-77 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 8.430699070992805 0.0 78-79 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 9.14876839181733 0.0 80-81 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 9.903338044208596 0.0 82-83 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 10.659897248351584 0.0 84-85 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 11.391348763277989 0.0 86-87 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 12.145804943135888 0.0 88 3.479824356622083E-4 6.0518684462992746E-5 1.0590769781023732E-4 12.738638411299807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12150 0.0 56.732292 1 GTATCAA 21830 0.0 40.97938 1 GTGGTAT 5030 0.0 33.510475 1 ATCAACG 27555 0.0 32.141117 3 TCAACGC 27990 0.0 31.692043 4 TATCAAC 28265 0.0 31.350403 2 CAACGCA 28930 0.0 30.688545 5 TGGTATC 5380 0.0 30.002237 2 AACGCAG 30260 0.0 29.416698 6 ACGCAGA 34300 0.0 25.882582 7 CGCAGAG 34600 0.0 25.549501 8 GCAGAGT 37250 0.0 23.49217 9 GTACATG 16350 0.0 22.980036 1 TACATGG 16640 0.0 21.776054 2 GAGTACT 28045 0.0 21.726353 12-13 CAGAGTA 36785 0.0 20.831465 10-11 ACATGGG 16835 0.0 20.713184 3 AGAGTAC 34920 0.0 20.67893 10-11 AGTACTT 29415 0.0 19.667944 12-13 GTACTTT 31180 0.0 19.29317 14-15 >>END_MODULE