FastQCFastQC Report
Wed 25 May 2016
SRR2049486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9872399
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG12300.045.880662
ATAACGC14700.037.4301953
TAACGCA16900.033.1139954
GGTATCA85850.032.4838831
GTATCAA124900.030.4606821
TACCTGG81850.029.1876132
TCAACGC130250.027.1513484
ATCAACG130300.027.141073
CAACGCA140950.025.3569855
GTATAAC28200.025.1814881
GTACCTG104700.023.4464651
AACGCAG155050.023.1891886
TATCAAC156450.023.1756732
GTACATG293600.022.3926051
TACATGG314850.020.3136862
ACATGGG302750.020.1158853
ACGCAGA180300.019.8882267
TATCACG17500.019.617262
ACCTGGG119850.019.580153
CGGATAC18050.018.7588674
Produced by FastQC (version 0.11.5)