##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049486_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9872399 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.91219672138454 31.0 30.0 34.0 26.0 34.0 2 30.043588189658866 31.0 30.0 34.0 26.0 34.0 3 30.13229276896122 31.0 30.0 34.0 26.0 34.0 4 33.78401855516577 35.0 35.0 37.0 28.0 37.0 5 33.78985978990517 37.0 35.0 37.0 29.0 37.0 6 33.83635436533714 37.0 35.0 37.0 30.0 37.0 7 33.71952318782902 37.0 35.0 37.0 29.0 37.0 8 33.66898916869142 37.0 35.0 37.0 29.0 37.0 9 35.0225131703044 38.0 35.0 39.0 28.0 39.0 10-11 34.93681247080876 38.0 35.0 39.0 27.5 39.0 12-13 34.83980226082839 38.0 35.0 39.0 27.0 39.0 14-15 35.722318860896934 39.0 36.0 41.0 27.0 41.0 16-17 35.75862680388019 39.0 36.0 41.0 27.0 41.0 18-19 35.82207146408892 39.0 36.0 41.0 27.0 41.0 20-21 35.95438601093817 39.0 36.0 41.0 27.0 41.0 22-23 35.887189223207045 39.0 36.0 41.0 27.0 41.0 24-25 35.79700263330119 39.0 35.5 41.0 27.0 41.0 26-27 35.64272184501457 39.0 35.0 41.0 27.0 41.0 28-29 35.55026002291844 39.0 35.0 41.0 27.0 41.0 30-31 35.52237789416736 39.0 35.0 41.0 27.0 41.0 32-33 35.41877860690193 39.0 35.0 41.0 26.5 41.0 34-35 35.35289983721282 39.0 35.0 40.5 26.0 41.0 36-37 35.24349253914879 39.0 35.0 40.0 25.5 41.0 38-39 35.10948909175977 39.0 34.5 40.0 25.0 41.0 40-41 35.07869682941299 39.0 34.0 40.0 25.5 41.0 42-43 34.938255686383826 39.0 34.0 40.0 24.5 41.0 44-45 34.827527584733964 38.0 34.0 40.0 24.5 41.0 46-47 34.59604403144565 38.0 34.0 40.0 24.0 41.0 48-49 34.52006082817358 38.0 33.5 40.0 24.0 41.0 50-51 34.431936199094054 38.0 33.0 40.0 24.0 41.0 52-53 34.25478518443187 38.0 33.0 40.0 23.5 41.0 54-55 34.19209631823025 38.0 33.0 40.0 24.0 41.0 56-57 33.959508727311366 38.0 33.0 40.0 23.0 41.0 58-59 33.77672990121246 37.0 33.0 40.0 23.0 41.0 60-61 33.48364045051259 37.0 33.0 40.0 22.0 41.0 62-63 33.18409299502583 36.0 32.0 39.5 22.0 41.0 64-65 32.966853396018536 36.0 32.0 39.0 22.0 41.0 66-67 32.662677430278094 35.5 32.0 39.0 20.5 41.0 68-69 32.367419155161784 35.0 32.0 39.0 20.0 40.0 70-71 32.075420877944666 35.0 32.0 38.0 19.5 40.0 72-73 31.735308155596222 35.0 31.0 37.0 18.0 40.0 74-75 31.376618135065243 35.0 31.0 37.0 17.5 39.0 76-77 31.10769742997624 35.0 31.0 36.0 15.0 39.0 78-79 30.80462413441758 35.0 31.0 36.0 12.0 38.5 80-81 30.467410656720823 34.5 31.0 35.5 7.5 37.0 82-83 30.178243201069975 34.0 30.0 35.0 6.0 37.0 84-85 29.919783175295084 34.0 30.0 35.0 2.0 36.5 86-87 29.696461974440055 34.0 30.0 35.0 2.0 36.0 88-89 29.44028903207822 34.0 30.0 35.0 2.0 36.0 90-91 29.11866877544151 34.0 30.0 35.0 2.0 35.5 92-93 28.92931596464041 34.0 30.0 35.0 2.0 35.0 94-95 28.677619340547317 34.0 29.0 35.0 2.0 35.0 96-97 28.41930228913965 34.0 29.0 35.0 2.0 35.0 98-99 28.149461088434535 34.0 29.0 35.0 2.0 35.0 100 27.98428031525063 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.533146642308882 1101 2 0.5378959486863621 1101 3 0.5023966801095128 1101 4 0.4822467568152504 1101 5 0.4459045486212361 1101 6 0.48030036398825615 1101 7 0.4804417491387767 1101 8 0.5163112075527536 1101 9 0.5982844956084605 1101 10-11 0.63645475532239 1101 12-13 0.5801029725867153 1101 14-15 0.8083074518116646 1101 16-17 0.8188233905077027 1101 18-19 0.7312638537866718 1101 20-21 0.6308615421552091 1101 22-23 0.5301066859703099 1101 24-25 0.5303394300185005 1101 26-27 0.6889553268988777 1101 28-29 0.7913256981027885 1101 30-31 0.766318805162463 1101 32-33 0.7554033472762995 1101 34-35 0.7555860777659049 1101 36-37 0.8255085744555544 1101 38-39 0.8611521255589452 1101 40-41 0.8009235279451374 1101 42-43 0.789093839701458 1101 44-45 0.7136071431862803 1101 46-47 0.8095654856963463 1101 48-49 0.6814043501311886 1101 50-51 0.6151843765020146 1101 52-53 0.6803080878205918 1101 54-55 0.5364375862995558 1101 56-57 0.5571668717181026 1101 58-59 0.45804018817349146 1101 60-61 0.5515588960488671 1101 62-63 0.5638112673995721 1101 64-65 0.5849755571685549 1101 66-67 0.6488091486245793 1101 68-69 0.5192126009853197 1101 70-71 0.515545488527593 1101 72-73 0.4686222703748726 1101 74-75 0.46713364143367997 1101 76-77 0.48916363291744247 1101 78-79 0.4523073954015224 1101 80-81 0.5251001857992215 1101 82-83 0.5739389730420008 1101 84-85 0.5647167050672124 1101 86-87 0.5545583519210915 1101 88-89 0.5652115231104773 1101 90-91 0.6083026774048435 1101 92-93 0.513973327991657 1101 94-95 0.4821359784928205 1101 96-97 0.44546161276941376 1101 98-99 0.3744964018529089 1101 100 0.40181702818077625 1102 1 0.3699893625148327 1102 2 0.44512518007809376 1102 3 0.4423846004697367 1102 4 0.4370862536597002 1102 5 0.424889586926966 1102 6 0.3256203616611586 1102 7 0.38857087621303776 1102 8 0.4535256512982997 1102 9 0.489518726986681 1102 10-11 0.5231196387008339 1102 12-13 0.559325628503835 1102 14-15 0.7960625942031072 1102 16-17 0.7748969861435242 1102 18-19 0.5999575079487869 1102 20-21 0.4806347048518873 1102 22-23 0.5250816760139472 1102 24-25 0.4905708774506863 1102 26-27 0.6297929822098638 1102 28-29 0.6938864319090996 1102 30-31 0.6333861179929059 1102 32-33 0.6164184434944033 1102 34-35 0.6147142689939855 1102 36-37 0.6640616577006568 1102 38-39 0.6885422123959728 1102 40-41 0.5359498994105465 1102 42-43 0.643362679471359 1102 44-45 0.5401829855396088 1102 46-47 0.6194042775254971 1102 48-49 0.6318191919975504 1102 50-51 0.4889600825029916 1102 52-53 0.5853093251202566 1102 54-55 0.44782146135086975 1102 56-57 0.4448956216762596 1102 58-59 0.4382099514403137 1102 60-61 0.37877625680186355 1102 62-63 0.4367693585573136 1102 64-65 0.40027110636956564 1102 66-67 0.4418378509454186 1102 68-69 0.45355913751814114 1102 70-71 0.40221002809062156 1102 72-73 0.38982748692826164 1102 74-75 0.4458569998915891 1102 76-77 0.40546432260437726 1102 78-79 0.44092683163160373 1102 80-81 0.48470127914773187 1102 82-83 0.5215488259162164 1102 84-85 0.46735230831154695 1102 86-87 0.4144074465907046 1102 88-89 0.37954317853742836 1102 90-91 0.4291914133233128 1102 92-93 0.37400743651446433 1102 94-95 0.3461752242377365 1102 96-97 0.22223394030455523 1102 98-99 0.17846661324002255 1102 100 0.1564064821185127 1103 1 0.24492852386541486 1103 2 0.29946543503377754 1103 3 0.2587960048532132 1103 4 0.19485233795422374 1103 5 0.2812088662623182 1103 6 0.1657463951730307 1103 7 0.13006818608806725 1103 8 0.255411264867881 1103 9 0.33512508619930514 1103 10-11 0.41955051309731317 1103 12-13 0.3463324764056779 1103 14-15 0.5951421745412802 1103 16-17 0.4329977362969828 1103 18-19 0.41135981171467506 1103 20-21 0.23467599402093953 1103 22-23 0.3373617813583678 1103 24-25 0.3399113077397402 1103 26-27 0.42594345481859364 1103 28-29 0.42130554826594135 1103 30-31 0.36282421257767794 1103 32-33 0.4332868058032915 1103 34-35 0.3685853805118384 1103 36-37 0.35119410933348405 1103 38-39 0.3570494327366873 1103 40-41 0.29553661287516064 1103 42-43 0.43607056457694426 1103 44-45 0.3436455809351102 1103 46-47 0.4477993105377962 1103 48-49 0.3549111449736415 1103 50-51 0.3402235282346382 1103 52-53 0.3261116596521134 1103 54-55 0.15963065226212336 1103 56-57 0.19221132138971342 1103 58-59 0.13989784112309422 1103 60-61 0.28041539620902967 1103 62-63 0.24752916602341202 1103 64-65 0.25831773761538557 1103 66-67 0.3016599413027876 1103 68-69 0.2524395534632049 1103 70-71 0.19737935718828936 1103 72-73 0.17143982014363246 1103 74-75 0.17416670189709293 1103 76-77 0.17684527706992625 1103 78-79 0.16107284032563385 1103 80-81 0.2250355942915192 1103 82-83 0.13145498895432794 1103 84-85 0.10700610736968486 1103 86-87 0.13040908184843758 1103 88-89 0.1280298448805084 1103 90-91 0.16250548845339807 1103 92-93 0.15602726559434998 1103 94-95 0.1980938196921116 1103 96-97 0.1414759216889081 1103 98-99 0.1375055718808298 1103 100 0.06751393112956094 1104 1 0.247183369121732 1104 2 0.2621768843666743 1104 3 0.29737554924475873 1104 4 0.2784272515754651 1104 5 0.20431490546703657 1104 6 0.2214612640117224 1104 7 0.17695217991482792 1104 8 0.15629584440508637 1104 9 0.39172615351359497 1104 10-11 0.3615586540712954 1104 12-13 0.36478836003134774 1104 14-15 0.5392455507089906 1104 16-17 0.4656252420415399 1104 18-19 0.38658555681007556 1104 20-21 0.3155145541847304 1104 22-23 0.308654854508859 1104 24-25 0.3849310520230631 1104 26-27 0.47312434654945434 1104 28-29 0.4017120431668175 1104 30-31 0.3426508685988523 1104 32-33 0.4278115795029933 1104 34-35 0.40960080199638327 1104 36-37 0.3555883746711359 1104 38-39 0.4488678546599232 1104 40-41 0.39660458967199475 1104 42-43 0.3258301027717323 1104 44-45 0.35049716846749135 1104 46-47 0.4880315344193349 1104 48-49 0.46338594685080636 1104 50-51 0.42227659544871443 1104 52-53 0.4168097808764415 1104 54-55 0.1893226494671012 1104 56-57 0.28464436370759216 1104 58-59 0.21576285926570193 1104 60-61 0.3036323513554322 1104 62-63 0.2841424024356769 1104 64-65 0.29646969798415057 1104 66-67 0.4024208236829949 1104 68-69 0.3129765189353577 1104 70-71 0.27872468705724174 1104 72-73 0.26196778721097047 1104 74-75 0.3200886054289853 1104 76-77 0.2829653097342266 1104 78-79 0.18018605959208145 1104 80-81 0.18973321509126606 1104 82-83 0.22327535173742774 1104 84-85 0.23197893649810553 1104 86-87 0.2213394017604564 1104 88-89 0.1935712568345913 1104 90-91 0.17112724981264194 1104 92-93 0.18244912855947248 1104 94-95 0.22020137048140143 1104 96-97 0.2030045848997588 1104 98-99 0.16920832088953475 1104 100 0.2601516525153045 1105 1 0.03568408465468664 1105 2 0.036570089232885294 1105 3 -0.006099745360476305 1105 4 0.0315267993615862 1105 5 -0.06662642340722158 1105 6 -0.020259018550177643 1105 7 -0.04923340003494303 1105 8 -0.009395859535054285 1105 9 0.09328009122689451 1105 10-11 0.09083434074736374 1105 12-13 0.10654832541045067 1105 14-15 0.1660365915296964 1105 16-17 0.11859282663195359 1105 18-19 0.14052132392470895 1105 20-21 -0.03897223127611937 1105 22-23 0.0065307954862205975 1105 24-25 0.04399417038487741 1105 26-27 0.1527990790343523 1105 28-29 0.17886873181723928 1105 30-31 0.08972422287305193 1105 32-33 0.20046599604182092 1105 34-35 0.19895577701161926 1105 36-37 0.16539028356877594 1105 38-39 0.1747227950763488 1105 40-41 0.14965184355663297 1105 42-43 0.1042082538404685 1105 44-45 0.1233670040421444 1105 46-47 0.26822469242991787 1105 48-49 0.165599176039251 1105 50-51 0.03984473303127345 1105 52-53 0.06924585496355462 1105 54-55 -0.07711499021641544 1105 56-57 -0.03704120777650388 1105 58-59 -0.11469965880665711 1105 60-61 -0.05882790601558696 1105 62-63 -0.020064992987556707 1105 64-65 -0.047127892392374804 1105 66-67 -0.042061258731386886 1105 68-69 -0.07187056166074512 1105 70-71 -0.10415659189165893 1105 72-73 -0.09815200750762898 1105 74-75 -0.014392707713852815 1105 76-77 -0.13259574686943765 1105 78-79 -0.14594952230847014 1105 80-81 -0.12030335843342499 1105 82-83 -0.08792026370925399 1105 84-85 -0.1412884771962517 1105 86-87 -0.11457865480933904 1105 88-89 -0.1948840891609258 1105 90-91 -0.14524442207574495 1105 92-93 -0.2471137236859171 1105 94-95 -0.34544579208444404 1105 96-97 -0.3572602717948925 1105 98-99 -0.3390487278830179 1105 100 -0.35034212227438033 1106 1 0.1291446623639665 1106 2 0.13098314540096823 1106 3 0.09666582769252585 1106 4 0.0995856539150779 1106 5 0.08174744416939461 1106 6 0.07470343465464424 1106 7 0.010189882504214154 1106 8 0.0603349857569242 1106 9 0.02884718058086122 1106 10-11 0.05729170623529001 1106 12-13 0.02588838606234134 1106 14-15 0.11673825731556775 1106 16-17 0.14148198310452642 1106 18-19 0.059740569880929684 1106 20-21 -0.011546068954274347 1106 22-23 -0.020753212236918728 1106 24-25 -0.029443365577371594 1106 26-27 0.07499873510252542 1106 28-29 0.0653384536553645 1106 30-31 0.08097751830550237 1106 32-33 0.05513193160529539 1106 34-35 0.13989260562419048 1106 36-37 0.1522425288742042 1106 38-39 0.07605454334152739 1106 40-41 0.0043372794160703165 1106 42-43 0.05665426363223247 1106 44-45 -0.01646025657767325 1106 46-47 0.19203892643708542 1106 48-49 0.1020663911245947 1106 50-51 -0.010553621661046009 1106 52-53 -0.016957996164862266 1106 54-55 -0.057562318476236385 1106 56-57 -0.13459250450491567 1106 58-59 -0.12629136411337782 1106 60-61 -0.13535981551702747 1106 62-63 -0.03982534437814422 1106 64-65 -0.10260598312404312 1106 66-67 -0.07616646490974688 1106 68-69 -0.1113797334713027 1106 70-71 -0.19276385276818786 1106 72-73 -0.2528762908889277 1106 74-75 -0.2483887233001454 1106 76-77 -0.21366689833399732 1106 78-79 -0.23576765727849391 1106 80-81 -0.12925959378480556 1106 82-83 -0.12128247841247486 1106 84-85 -0.14881113073548136 1106 86-87 -0.09723259174550591 1106 88-89 -0.129199561043837 1106 90-91 -0.11643374565656117 1106 92-93 -0.21338119239175768 1106 94-95 -0.19878405694787915 1106 96-97 -0.2795711226586235 1106 98-99 -0.2639039950681017 1106 100 -0.2175842226420137 1107 1 -0.09248298438382108 1107 2 -0.08804807478692922 1107 3 -0.1296135947805972 1107 4 -0.09539594636815707 1107 5 -0.13134215361630197 1107 6 -0.17563861626216948 1107 7 -0.09452846747713295 1107 8 -0.07695574527821947 1107 9 0.04204262006503967 1107 10-11 0.002870559676566131 1107 12-13 -0.06197060548847588 1107 14-15 0.13650574691408934 1107 16-17 0.05343691802305983 1107 18-19 -0.024286482710174084 1107 20-21 -0.14223994192406053 1107 22-23 -0.07602474339115872 1107 24-25 -0.0018589531537571702 1107 26-27 0.11207759511755455 1107 28-29 -0.016059817804439547 1107 30-31 -0.10355241577428131 1107 32-33 -0.14637204034918483 1107 34-35 -0.12126491975030973 1107 36-37 -0.12440879883482836 1107 38-39 -0.0450789776649998 1107 40-41 -0.1488565851454169 1107 42-43 -0.10274725907285642 1107 44-45 -0.07116681270622394 1107 46-47 -0.012199097138896775 1107 48-49 -0.06713164550883732 1107 50-51 -0.08076514022758374 1107 52-53 -0.06708630399937476 1107 54-55 -0.1412368271516442 1107 56-57 -0.18509326515700053 1107 58-59 -0.2601923214334434 1107 60-61 -0.24100700437035272 1107 62-63 -0.2046057103455894 1107 64-65 -0.2651497629034907 1107 66-67 -0.18509159712640155 1107 68-69 -0.15811873317413472 1107 70-71 -0.33115392348377526 1107 72-73 -0.3111642326209001 1107 74-75 -0.18939048198235042 1107 76-77 -0.24594011773531932 1107 78-79 -0.27479191233880584 1107 80-81 -0.1674320674994263 1107 82-83 -0.17712884523175987 1107 84-85 -0.23911724945469004 1107 86-87 -0.21557635789934082 1107 88-89 -0.23864771018862996 1107 90-91 -0.20878765302281366 1107 92-93 -0.19647005691835417 1107 94-95 -0.1600902549135519 1107 96-97 -0.2166723626420115 1107 98-99 -0.2830824660805611 1107 100 -0.34082724641686823 1108 1 0.022431792492369595 1108 2 3.956608095805336E-4 1108 3 0.003924673385927946 1108 4 0.0034283363838838454 1108 5 -0.032687427173947015 1108 6 -0.0016828486690627642 1108 7 -0.023694608215379276 1108 8 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2215 72-73 -0.05605539834026274 2215 74-75 -0.08152114066726313 2215 76-77 -0.04730457322780168 2215 78-79 -0.018844800094271363 2215 80-81 -0.08943207929125307 2215 82-83 0.00990611143805964 2215 84-85 -0.06462383314922349 2215 86-87 -0.056848941956801013 2215 88-89 -0.05347825583829291 2215 90-91 -0.0012362526249241057 2215 92-93 -0.0880202830719945 2215 94-95 -0.04699117529589003 2215 96-97 6.285858996122329E-4 2215 98-99 0.06820621684441619 2215 100 0.10494458485337432 2216 1 -0.3799085812346874 2216 2 -0.3409655694783069 2216 3 -0.33545545014365885 2216 4 -0.426664504646709 2216 5 -0.4218324964192419 2216 6 -0.28778893513467807 2216 7 -0.3735233993664835 2216 8 -0.31142457290017234 2216 9 -0.17289546037720527 2216 10-11 -0.37731656028527283 2216 12-13 -0.35139491302045656 2216 14-15 -0.13433244073997486 2216 16-17 -0.3788616927037509 2216 18-19 -0.38680526778605895 2216 20-21 -0.2954976316100897 2216 22-23 -0.46281046801303916 2216 24-25 -0.3766437757125658 2216 26-27 -0.3441531548594128 2216 28-29 -0.3097223746914608 2216 30-31 -0.28392489904032203 2216 32-33 -0.4440030854139465 2216 34-35 -0.7279180829187339 2216 36-37 -0.6531912970514284 2216 38-39 -0.539454216041861 2216 40-41 -0.6857522199440425 2216 42-43 -0.5205378697620375 2216 44-45 -0.6855444600290284 2216 46-47 -0.3613120516372206 2216 48-49 -0.3230161599623358 2216 50-51 -0.3165855972241616 2216 52-53 -0.4134033836884612 2216 54-55 -0.49092153092212243 2216 56-57 -0.4119670309405379 2216 58-59 -0.5378740811070983 2216 60-61 -0.42175980280224934 2216 62-63 -0.3534644681698751 2216 64-65 -0.5525502813677505 2216 66-67 -0.5071689912959556 2216 68-69 -0.5100564674111325 2216 70-71 -0.5406046426376925 2216 72-73 -0.545738181186433 2216 74-75 -0.5330790779281287 2216 76-77 -0.5729247987685575 2216 78-79 -0.4547053234873921 2216 80-81 -0.4422784901319581 2216 82-83 -0.37630888734068435 2216 84-85 -0.3975237523009234 2216 86-87 -0.38370463475504124 2216 88-89 -0.36063331778610674 2216 90-91 -0.35578606924580214 2216 92-93 -0.5449854349987469 2216 94-95 -0.43897644517482703 2216 96-97 -0.4598864551695101 2216 98-99 -0.3586932466948909 2216 100 -0.32019441857872266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 253842.0 3 40546.0 4 27172.0 5 21569.0 6 20044.0 7 21260.0 8 23319.0 9 25814.0 10 28432.0 11 30634.0 12 32865.0 13 34074.0 14 35319.0 15 36113.0 16 36898.0 17 37492.0 18 37573.0 19 38110.0 20 39372.0 21 41907.0 22 48734.0 23 60078.0 24 75218.0 25 94879.0 26 116253.0 27 138640.0 28 168627.0 29 209604.0 30 254242.0 31 310053.0 32 389175.0 33 500157.0 34 663105.0 35 914218.0 36 1350756.0 37 1852639.0 38 1534044.0 39 329243.0 40 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54109997877774 18.405546517945798 13.039549698445013 24.013803804831454 2 17.597590132856205 19.559340898495304 37.08761386931706 25.75545509933143 3 19.08416525153152 24.428445362864732 29.08330490888383 27.40408447671992 4 13.87489309745119 16.98650935238134 35.79340334797121 33.34519420219626 5 14.30224694075214 35.58150189422478 32.73782579423244 17.37842537079063 6 31.803944855497875 35.302982643682284 18.026905020192352 14.86616748062749 7 28.31084438009373 30.73860698272873 21.600193457722188 19.35035517945536 8 26.444382346929174 33.60717188448024 20.202514057110967 19.74593171147962 9 25.45084509438481 17.371971511079575 20.75115265029978 36.42603074423584 10-11 24.9545253994729 25.769702348437935 27.394546170482055 21.881226081607114 12-13 25.443997384073363 23.535964581383308 27.28326631144916 23.73677172309417 14-15 23.24020049316355 25.354053271557454 24.667382230946682 26.738364004332315 16-17 23.28098527065705 28.01412795493199 24.822797350486344 23.88208942392462 18-19 23.20935739197062 27.453470041982797 26.285900332167966 23.051272233878617 20-21 24.344038234129812 26.952045646579627 25.6533361853153 23.050579933975264 22-23 23.943401046360396 26.968540255274633 25.57136224526517 23.5166964530998 24-25 23.601084855436156 26.87323908538956 25.936390823686967 23.589285235487317 26-27 23.6801909636903 26.627302909544454 25.914260563094732 23.77824556367051 28-29 23.639492398835973 26.72654663911085 26.004311374234824 23.62964958781835 30-31 23.836647391693614 27.23467000860834 25.50292876168691 23.42575383801114 32-33 23.57911620818006 26.953264161562196 25.873501936377984 23.594117693879753 34-35 23.53760795811234 26.40410548225134 26.00521761008278 24.05306894955354 36-37 24.03255783812596 26.70516102004053 25.874557978684404 23.387723163149104 38-39 23.79885602632753 26.812024860566297 25.806542188134486 23.582576924971683 40-41 23.723246621829666 26.53329944244762 25.937348659920424 23.806105275802288 42-43 23.32081382086048 26.788980510752108 26.123279982160795 23.76692568622661 44-45 23.563078242785796 26.821349754089173 25.928476224902568 23.687095778222456 46-47 23.52961720106662 26.812804512630006 25.908253009828293 23.749325276475076 48-49 23.38298080591603 26.585027617983226 26.254127820627797 23.777863755472946 50-51 23.38151289092196 26.487514760080177 26.112274076435995 24.01869827256187 52-53 23.34534047893058 26.5170950664777 26.111469350558426 24.026095104033292 54-55 23.365462551217043 26.901676707395684 26.0124676981729 23.72039304321437 56-57 22.94962592570837 26.70357632345578 26.08469482482121 24.262102926014638 58-59 23.078080934761946 26.277463968848025 26.294452121436102 24.35000297495393 60-61 23.431830825465134 26.65633053615518 26.000569750813334 23.91126888756635 62-63 23.229229866538596 26.787122803355963 25.969296715584118 24.014350614521323 64-65 23.265607810345493 26.298449817681806 26.128414224746916 24.30752814722579 66-67 22.92186690505024 26.493819316590734 26.15198594918185 24.43232782917718 68-69 23.41951075787202 26.448212118627147 25.979566650056945 24.15271047344389 70-71 23.315954001539446 26.15831208815449 26.20476611752074 24.32096779278533 72-73 23.017061688778266 26.43070216910755 26.39000849711041 24.162227645003775 74-75 23.061782379787292 26.2357302097209 26.336914590023337 24.36557282046848 76-77 23.212567198074694 26.297566262466248 26.027202790241855 24.4626637492172 78-79 23.213477406900818 26.54670717745317 26.285085292059463 23.95473012358655 80-81 23.147602865448338 26.254041659868584 26.393475219017382 24.2048802556657 82-83 23.18269001861984 26.19896059949639 26.41036426356639 24.207985118317374 84-85 23.26666510221218 26.30131484627942 26.215967603019706 24.21605244848869 86-87 23.464532704271857 26.308339901402682 26.199037361185496 24.028090033139968 88-89 23.300083397256024 26.08665661642305 26.3728376834974 24.240422302823518 90-91 23.1932412767491 26.032331821358934 26.357013498287053 24.417413403604915 92-93 23.44830704108095 26.190814682992997 26.174666960832955 24.186211315093097 94-95 23.541603131763665 25.85145470831708 26.292636344233483 24.31430581568577 96-97 23.308888602467896 25.910286153325124 26.424257531150268 24.356567713056716 98-99 23.455060550235537 25.988348609207083 26.383239025824125 24.17335181473326 100 23.62284472010908 25.76228195826946 26.376182218684963 24.238691102936496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10547.0 1 7133.5 2 4618.0 3 5313.0 4 4637.5 5 3893.0 6 3482.5 7 3317.5 8 3238.0 9 3155.5 10 2943.0 11 2692.5 12 2641.0 13 2648.5 14 2690.0 15 2827.0 16 3066.0 17 3500.0 18 4079.5 19 4914.0 20 6241.5 21 8128.0 22 10822.5 23 14683.0 24 20265.5 25 27800.5 26 36814.0 27 48349.5 28 63228.0 29 80093.5 30 95593.0 31 110401.0 32 125125.0 33 138454.5 34 153610.0 35 170503.5 36 188400.0 37 208882.0 38 229025.5 39 244551.5 40 259769.0 41 275286.5 42 286103.0 43 295711.0 44 305314.0 45 314981.0 46 326013.5 47 332399.0 48 337263.5 49 348320.0 50 360199.0 51 367998.5 52 371880.0 53 378320.0 54 382258.0 55 371428.5 56 349745.5 57 321045.0 58 292270.0 59 267882.5 60 239560.0 61 208314.0 62 178694.5 63 147924.0 64 118261.0 65 93368.5 66 72263.5 67 54472.5 68 40690.5 69 30779.0 70 23076.5 71 17328.0 72 13449.0 73 10239.0 74 7928.5 75 5494.5 76 3152.0 77 1928.0 78 1296.0 79 891.5 80 585.5 81 345.5 82 232.5 83 166.0 84 118.5 85 97.0 86 80.5 87 70.0 88 59.5 89 56.5 90 44.0 91 32.0 92 27.0 93 17.0 94 17.0 95 17.5 96 17.0 97 16.5 98 13.5 99 13.0 100 23.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15018639339840295 2 0.16464083349953745 3 0.16066003815283397 4 0.1667274590502268 5 0.17669464129235457 6 0.1088387938939664 7 0.09905393815626778 8 0.09901342115528353 9 0.09599490458195623 10-11 0.08762814387870668 12-13 0.08314088601969997 14-15 0.0802540496995715 16-17 0.0866152188541002 18-19 0.11724100697307716 20-21 0.08016288644735692 22-23 0.07828897515183493 24-25 0.0777369310134244 26-27 0.07583769659228724 28-29 0.07425753355390113 30-31 0.09996557067841363 32-33 0.10172299559610587 34-35 0.0744094723075921 36-37 0.11883636388683237 38-39 0.07229245900616456 40-41 0.07502229194747903 42-43 0.08842329002302277 44-45 0.10214335948131757 46-47 0.08279142688621074 48-49 0.08187979436406491 50-51 0.07627831897799106 52-53 0.07114278910323621 54-55 0.06750638826489894 56-57 0.06866112279295032 58-59 0.06786597664863424 60-61 0.0676887147693281 62-63 0.07128459860668111 64-65 0.06905616355254686 66-67 0.07157328223869396 68-69 0.07791925751785356 70-71 0.07908918592127405 72-73 0.08566813395609314 74-75 0.07333577178150923 76-77 0.07253556101207012 78-79 0.07300657114851213 80-81 0.07582250271691815 82-83 0.07480957769231167 84-85 0.07501216269723296 86-87 0.07212532637710449 88-89 0.07056542183921051 90-91 0.07914996142275044 92-93 0.07323954390417162 94-95 0.07510332594944755 96-97 0.07450570018492972 98-99 0.06709615362993332 100 0.0671265413806715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9872399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.13978329630202 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25311738939527 41.05213917036246 2 12.542406132852907 13.330014878280261 3 3.9370112014980405 6.276357662481587 4 1.8257807374195931 3.880863709321588 5 1.0622197132626177 2.8223062687917793 6 0.6463611772265206 2.0608495733375984 7 0.44590594869225186 1.6586741838826764 8 0.3330410983909826 1.4158185437807376 9 0.23945237126519237 1.1452002406936181 >10 1.5297354376385277 15.072399956789898 >50 0.11986544172477676 4.377114622247302 >100 0.06271332262248794 5.808576704204886 >500 0.0020653319183477088 0.7421863378099172 >1k 2.864966387109821E-4 0.2141154057372454 >5k 3.819945385278564E-5 0.14338274227854483 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0129250246064812E-5 0.0 2 0.0 0.0 0.0 1.0129250246064812E-5 0.0 3 0.0 0.0 0.0 1.0129250246064812E-5 0.0 4 0.0 0.0 0.0 2.0258500492129625E-5 0.0 5 0.0 0.0 0.0 3.0387750738194435E-5 0.0 6 0.0 0.0 0.0 4.051700098425925E-5 0.0 7 0.0 1.0129250246064812E-5 0.0 4.051700098425925E-5 0.0 8 0.0 1.0129250246064812E-5 0.0 5.0646251230324056E-5 0.0 9 0.0 1.0129250246064812E-5 0.0 1.1142175270671293E-4 0.0 10-11 0.0 1.5193875369097218E-5 0.0 1.1142175270671293E-4 0.0 12-13 0.0 2.0258500492129625E-5 0.0 1.2155100295277774E-4 0.0 14-15 0.0 2.532312561516203E-5 0.0 1.721972541831018E-4 0.0 16-17 0.0 3.0387750738194435E-5 1.0129250246064812E-5 2.0258500492129623E-4 0.0 18-19 1.5193875369097218E-5 3.545237586122684E-5 1.0129250246064812E-5 2.582958812746527E-4 0.0 20-21 3.0387750738194435E-5 4.051700098425925E-5 1.0129250246064812E-5 4.051700098425925E-4 0.0 22-23 3.0387750738194435E-5 4.558162610729165E-5 1.0129250246064812E-5 0.0010534420255907404 0.0 24-25 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 0.0023905030580712956 0.0 26-27 3.0387750738194435E-5 8.10340019685185E-5 1.0129250246064812E-5 0.004750618365404397 5.064625123032406E-6 28-29 3.0387750738194435E-5 8.10340019685185E-5 2.0258500492129625E-5 0.011056076643579743 1.0129250246064812E-5 30-31 3.0387750738194435E-5 8.10340019685185E-5 2.0258500492129625E-5 0.02961286309437048 1.0129250246064812E-5 32-33 3.0387750738194435E-5 8.10340019685185E-5 2.0258500492129625E-5 0.07181638424459952 1.0129250246064812E-5 34-35 3.0387750738194435E-5 8.60986270915509E-5 2.0258500492129625E-5 0.1357066301716533 1.0129250246064812E-5 36-37 3.0387750738194435E-5 1.0635712758368053E-4 2.0258500492129625E-5 0.23139259262110456 1.0129250246064812E-5 38-39 3.0387750738194435E-5 1.2661562807581014E-4 2.0258500492129625E-5 0.3677221716828909 2.0258500492129625E-5 40-41 3.0387750738194435E-5 1.3168025319884255E-4 2.0258500492129625E-5 0.5335177397104797 2.0258500492129625E-5 42-43 3.0387750738194435E-5 1.4180950344490736E-4 2.0258500492129625E-5 0.7070672488014311 2.0258500492129625E-5 44-45 3.545237586122684E-5 1.4180950344490736E-4 2.0258500492129625E-5 0.9107107603734411 2.0258500492129625E-5 46-47 4.051700098425925E-5 1.4180950344490736E-4 2.0258500492129625E-5 1.1510778687125591 2.0258500492129625E-5 48-49 4.051700098425925E-5 1.4180950344490736E-4 2.0258500492129625E-5 1.4133393514585464 2.0258500492129625E-5 50-51 4.051700098425925E-5 1.4180950344490736E-4 2.0258500492129625E-5 1.6882674616372373 2.0258500492129625E-5 52-53 4.051700098425925E-5 1.4180950344490736E-4 2.0258500492129625E-5 1.966239411514871 2.0258500492129625E-5 54-55 4.051700098425925E-5 1.4180950344490736E-4 2.0258500492129625E-5 2.264976324396937 2.0258500492129625E-5 56-57 4.051700098425925E-5 1.4687412856793976E-4 2.0258500492129625E-5 2.602629816724385 2.0258500492129625E-5 58-59 4.051700098425925E-5 1.6206800393703697E-4 3.0387750738194435E-5 2.96449728176505 2.0258500492129625E-5 60-61 4.051700098425925E-5 1.721972541831018E-4 4.051700098425925E-5 3.337051105815314 2.0258500492129625E-5 62-63 4.051700098425925E-5 1.721972541831018E-4 4.051700098425925E-5 3.7134844327098206 2.0258500492129625E-5 64-65 4.051700098425925E-5 1.721972541831018E-4 4.051700098425925E-5 4.120346027343507 2.0258500492129625E-5 66-67 4.051700098425925E-5 1.9245575467523142E-4 4.051700098425925E-5 4.570606394656457 2.0258500492129625E-5 68-69 4.051700098425925E-5 2.0258500492129623E-4 4.051700098425925E-5 5.0387296947783415 2.0258500492129625E-5 70-71 4.051700098425925E-5 2.1271425516736106E-4 4.051700098425925E-5 5.523115506170283 2.0258500492129625E-5 72-73 4.051700098425925E-5 2.2284350541342587E-4 4.051700098425925E-5 6.039818690472295 2.0258500492129625E-5 74-75 4.051700098425925E-5 2.3803738078252308E-4 4.051700098425925E-5 6.540153006376666 2.0258500492129625E-5 76-77 4.051700098425925E-5 2.532312561516203E-4 4.051700098425925E-5 7.064331577360274 2.0258500492129625E-5 78-79 4.051700098425925E-5 2.532312561516203E-4 4.051700098425925E-5 7.623724486824327 2.0258500492129625E-5 80-81 4.051700098425925E-5 2.633605063976851E-4 4.051700098425925E-5 8.206242474600145 2.0258500492129625E-5 82-83 4.051700098425925E-5 2.7855438176678233E-4 4.051700098425925E-5 8.818008672461476 2.0258500492129625E-5 84-85 4.051700098425925E-5 2.836190068898147E-4 4.051700098425925E-5 9.412783053035032 3.0387750738194435E-5 86-87 4.051700098425925E-5 2.836190068898147E-4 4.051700098425925E-5 10.021368666319098 3.0387750738194435E-5 88 4.051700098425925E-5 2.836190068898147E-4 4.051700098425925E-5 10.503890695665765 3.0387750738194435E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 1230 0.0 45.88066 2 ATAACGC 1470 0.0 37.430195 3 TAACGCA 1690 0.0 33.113995 4 GGTATCA 8585 0.0 32.483883 1 GTATCAA 12490 0.0 30.460682 1 TACCTGG 8185 0.0 29.187613 2 TCAACGC 13025 0.0 27.151348 4 ATCAACG 13030 0.0 27.14107 3 CAACGCA 14095 0.0 25.356985 5 GTATAAC 2820 0.0 25.181488 1 GTACCTG 10470 0.0 23.446465 1 AACGCAG 15505 0.0 23.189188 6 TATCAAC 15645 0.0 23.175673 2 GTACATG 29360 0.0 22.392605 1 TACATGG 31485 0.0 20.313686 2 ACATGGG 30275 0.0 20.115885 3 ACGCAGA 18030 0.0 19.888226 7 TATCACG 1750 0.0 19.61726 2 ACCTGGG 11985 0.0 19.58015 3 CGGATAC 1805 0.0 18.758867 4 >>END_MODULE