FastQCFastQC Report
Wed 25 May 2016
SRR2049486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9872399
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231200.23418826568901846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218370.22119243762331728No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206820.20949315358911244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142290.1441291017512562No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99800.10108991745572682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG15450.051.7179832
GGTATCA149150.049.022751
ATAACGC17700.047.0025143
TAACGCA20200.042.1161164
GTATCAA246900.038.507971
ATCAACG302100.030.899373
TCAACGC305400.030.611664
TACCTGG83000.029.7305092
GTATAAC34650.029.718811
CAACGCA319350.029.2008765
TATCAAC321950.029.081852
AACGCAG341000.027.7733736
ACGCAGA386650.024.4804047
CGCAGAG399050.023.6843728
TATCACG20300.023.3854892
GTACCTG108850.023.3268361
GCAGAGT438350.021.4323069
GTGGTAT77600.021.3896431
AGAGTAC400100.021.16132510-11
ACCTGGG115700.020.6372323
Produced by FastQC (version 0.11.5)