##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9872399 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.216750153635402 31.0 31.0 34.0 28.0 34.0 2 31.30365993108666 31.0 31.0 34.0 28.0 34.0 3 31.493299855485986 31.0 31.0 34.0 28.0 34.0 4 35.1936564759994 37.0 35.0 37.0 32.0 37.0 5 35.277501750081214 37.0 35.0 37.0 33.0 37.0 6 35.34487301414783 37.0 35.0 37.0 33.0 37.0 7 35.29108740438874 37.0 35.0 37.0 32.0 37.0 8 35.27428905578067 37.0 35.0 37.0 32.0 37.0 9 36.88604279466419 39.0 37.0 39.0 33.0 39.0 10-11 36.764063324426004 39.0 37.0 39.0 32.0 39.0 12-13 36.70835609460274 39.0 37.0 39.0 32.0 39.0 14-15 37.848470468018974 40.0 37.0 41.0 33.0 41.0 16-17 37.84802574328692 40.0 37.0 41.0 33.0 41.0 18-19 37.88477967715851 40.0 37.0 41.0 33.0 41.0 20-21 37.93898630920407 40.0 37.0 41.0 33.0 41.0 22-23 37.8521066662723 40.0 37.0 41.0 32.5 41.0 24-25 37.69181680156971 40.0 37.0 41.0 32.0 41.0 26-27 37.653828871786885 39.5 37.0 41.0 32.0 41.0 28-29 37.60214194138628 40.0 37.0 41.0 32.0 41.0 30-31 37.49552651792133 39.0 36.5 41.0 32.0 41.0 32-33 37.37238142421107 39.0 36.0 41.0 31.5 41.0 34-35 37.25829056341827 39.0 36.0 41.0 31.0 41.0 36-37 37.12996233235711 39.0 36.0 41.0 31.0 41.0 38-39 37.03537964784446 39.0 36.0 41.0 31.0 41.0 40-41 36.93223946884643 39.0 35.5 41.0 31.0 41.0 42-43 36.754828233745414 39.0 35.0 41.0 30.0 41.0 44-45 36.62870817923789 39.0 35.0 41.0 30.0 41.0 46-47 36.431629282811606 39.0 35.0 40.5 30.0 41.0 48-49 36.18879180227623 39.0 35.0 40.0 30.0 41.0 50-51 36.01436499882146 39.0 35.0 40.0 29.0 41.0 52-53 35.878427573682956 38.5 35.0 40.0 29.0 41.0 54-55 35.704659981834205 38.0 35.0 40.0 28.5 41.0 56-57 35.52806161906544 38.0 34.0 40.0 28.0 41.0 58-59 35.297657489329595 38.0 34.0 40.0 28.0 41.0 60-61 35.069533909640406 37.0 34.0 40.0 28.0 41.0 62-63 34.80069616311091 37.0 34.0 40.0 28.0 41.0 64-65 34.53628191081013 36.0 34.0 39.0 28.0 41.0 66-67 34.19484727065833 36.0 33.0 39.0 27.0 41.0 68-69 33.90530452628586 35.0 33.0 39.0 26.5 40.5 70-71 33.62383940316837 35.0 33.0 38.5 26.0 40.0 72-73 33.304053047288704 35.0 33.0 37.0 26.0 40.0 74-75 33.01424537237605 35.0 33.0 37.0 26.0 39.0 76-77 32.727344285821516 35.0 33.0 36.5 26.0 39.0 78-79 32.46662128424914 35.0 32.0 36.0 26.0 39.0 80-81 32.1612762004453 35.0 32.0 35.5 25.0 37.5 82-83 31.963561946797327 35.0 32.0 35.0 25.0 37.0 84-85 31.686892365270083 35.0 32.0 35.0 25.0 36.5 86-87 31.526392318624886 35.0 32.0 35.0 25.0 36.0 88-89 31.37022328615365 34.0 32.0 35.0 24.0 36.0 90-91 31.208474758769373 34.0 32.0 35.0 24.0 36.0 92-93 30.958394357845545 34.0 32.0 35.0 23.0 35.0 94-95 30.816983085874064 34.0 32.0 35.0 23.0 35.0 96-97 30.657520932855327 34.0 32.0 35.0 21.5 35.0 98-99 30.451428826975082 34.0 31.0 35.0 19.5 35.0 100 30.319577946555846 34.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13423159195956558 1101 2 0.1705030375693113 1101 3 0.21852742691626048 1101 4 0.2933912467962543 1101 5 0.26095550562964576 1101 6 0.23999765993600874 1101 7 0.19555858449191277 1101 8 0.21798465023950087 1101 9 0.2806164681728873 1101 10-11 0.3450387387034297 1101 12-13 0.3618051582656605 1101 14-15 0.3437809342677056 1101 16-17 0.3707854083060269 1101 18-19 0.4252906243954868 1101 20-21 0.36241366620242843 1101 22-23 0.34395235210403285 1101 24-25 0.47103808340578013 1101 26-27 0.37308256613206225 1101 28-29 0.2890288306307056 1101 30-31 0.26070607866414974 1101 32-33 0.266171852286611 1101 34-35 0.31514550136677855 1101 36-37 0.3356978832481161 1101 38-39 0.35299862041699726 1101 40-41 0.34591462371626847 1101 42-43 0.37918928296301146 1101 44-45 0.29395543359247256 1101 46-47 0.3272883510987583 1101 48-49 0.38920226475740094 1101 50-51 0.39833803560186 1101 52-53 0.4054274137964171 1101 54-55 0.35196443182762494 1101 56-57 0.3760055605327466 1101 58-59 0.42661103276449097 1101 60-61 0.3727885003048499 1101 62-63 0.3665252134187327 1101 64-65 0.37175026454413995 1101 66-67 0.3754428690924385 1101 68-69 0.38459988726549454 1101 70-71 0.38370558893562645 1101 72-73 0.3350239080293491 1101 74-75 0.3389763089504143 1101 76-77 0.356160886075088 1101 78-79 0.35204700652102616 1101 80-81 0.3588732371783365 1101 82-83 0.34215211558086267 1101 84-85 0.4139946196028035 1101 86-87 0.3645761507406675 1101 88-89 0.3088030225560523 1101 90-91 0.3592272038255757 1101 92-93 0.4404617929377892 1101 94-95 0.33744023513855836 1101 96-97 0.3054896379665166 1101 98-99 0.24948312701610575 1101 100 0.2698284071134651 1102 1 0.10971650703895008 1102 2 0.16148313221617627 1102 3 0.19924594379457972 1102 4 0.23599297783034956 1102 5 0.19547597644291415 1102 6 0.2093547722793616 1102 7 0.19573755736302445 1102 8 0.23498679697451763 1102 9 0.2443944248573544 1102 10-11 0.2899750708199704 1102 12-13 0.2649539374899561 1102 14-15 0.38583932169844815 1102 16-17 0.3561137489746855 1102 18-19 0.3839703481255654 1102 20-21 0.24836448855030113 1102 22-23 0.221936399921141 1102 24-25 0.42655193786430345 1102 26-27 0.2632500641879787 1102 28-29 0.3403343466628215 1102 30-31 0.3218117382832659 1102 32-33 0.2873172168718199 1102 34-35 0.34897640291306686 1102 36-37 0.38327681791601265 1102 38-39 0.3025328372126097 1102 40-41 0.35105363511561904 1102 42-43 0.36960030014923717 1102 44-45 0.2848877039996438 1102 46-47 0.3814566025849899 1102 48-49 0.4206397945767293 1102 50-51 0.4474610404470738 1102 52-53 0.3981076223933968 1102 54-55 0.36689418515730665 1102 56-57 0.34752601998557964 1102 58-59 0.40771003708571385 1102 60-61 0.3819661951414446 1102 62-63 0.41259063874130675 1102 64-65 0.4057340767847393 1102 66-67 0.41513272182797323 1102 68-69 0.4300806472145524 1102 70-71 0.39315233870277666 1102 72-73 0.41102355691148773 1102 74-75 0.36774577063396663 1102 76-77 0.3445607211056725 1102 78-79 0.2836885559221898 1102 80-81 0.3132403268028483 1102 82-83 0.2684796394312663 1102 84-85 0.42651718563688945 1102 86-87 0.32040936129315867 1102 88-89 0.2602779611387547 1102 90-91 0.325197298528515 1102 92-93 0.4287070974676439 1102 94-95 0.31308945524559206 1102 96-97 0.31576933868130297 1102 98-99 0.2892783180147198 1102 100 0.2393600033576817 1103 1 0.018219165469883336 1103 2 0.046717055562087495 1103 3 0.05491330329623523 1103 4 0.19837630631514003 1103 5 0.15718302181122823 1103 6 0.11979457540526539 1103 7 0.12206540962225887 1103 8 0.14759674197364347 1103 9 0.12509346787680187 1103 10-11 0.21512349825329835 1103 12-13 0.237664711543772 1103 14-15 0.24937203921864182 1103 16-17 0.23332281101070151 1103 18-19 0.2041405763904649 1103 20-21 0.22298181649993154 1103 22-23 0.16841629677488612 1103 24-25 0.3532297358979335 1103 26-27 0.14350284057760376 1103 28-29 0.16458238845611106 1103 30-31 0.22133309872975104 1103 32-33 0.21948547060272716 1103 34-35 0.2430776950491662 1103 36-37 0.20244191197349437 1103 38-39 0.18016045657579838 1103 40-41 0.16845621623100016 1103 42-43 0.24993537701457313 1103 44-45 0.1661387943291288 1103 46-47 0.19461133526492347 1103 48-49 0.25742751882290804 1103 50-51 0.3652764415421714 1103 52-53 0.298569991048268 1103 54-55 0.23317365353373276 1103 56-57 0.16963759301087578 1103 58-59 0.14918002476088787 1103 60-61 0.1482956781680116 1103 62-63 0.21015262366261567 1103 64-65 0.1399461965506532 1103 66-67 0.1712290768839253 1103 68-69 0.16163936596561967 1103 70-71 0.14916243273744811 1103 72-73 0.13878187693989474 1103 74-75 0.1640226153727511 1103 76-77 0.22465968461516894 1103 78-79 0.14532632295314585 1103 80-81 0.13767617394131548 1103 82-83 0.13761881526239605 1103 84-85 0.061828925114458144 1103 86-87 0.08231396893284426 1103 88-89 0.08949193916748754 1103 90-91 0.04130062811248081 1103 92-93 0.1388506309779487 1103 94-95 0.03743965927562343 1103 96-97 0.07814801902916102 1103 98-99 0.04980655501833553 1103 100 0.01817273650637219 1104 1 -0.025072698002382765 1104 2 -0.029641470520946456 1104 3 0.058707698307589595 1104 4 0.16337002021532498 1104 5 0.10300418491907948 1104 6 0.12603621740255733 1104 7 0.108012576524402 1104 8 0.2040584686138942 1104 9 0.20283014908763164 1104 10-11 0.25339398637348864 1104 12-13 0.15551674981286112 1104 14-15 0.11891547153318527 1104 16-17 0.21523888431156735 1104 18-19 0.22075572706906854 1104 20-21 0.14274587232596758 1104 22-23 0.05212208313594857 1104 24-25 0.25128751604227517 1104 26-27 0.15203849027740546 1104 28-29 0.14553726957633728 1104 30-31 0.20371239602557267 1104 32-33 0.1815202830034579 1104 34-35 0.188070470245016 1104 36-37 0.18765007782408105 1104 38-39 0.22928665440941387 1104 40-41 0.19554455693998563 1104 42-43 0.19739316552887232 1104 44-45 0.12016123104228171 1104 46-47 0.1916487200047925 1104 48-49 0.2243633242767089 1104 50-51 0.26600114508166683 1104 52-53 0.2158350125220636 1104 54-55 0.18598239266579242 1104 56-57 0.16964033634162945 1104 58-59 0.13588511376541135 1104 60-61 0.19220389761152035 1104 62-63 0.2252049202713522 1104 64-65 0.19512114943698577 1104 66-67 0.16012225209752273 1104 68-69 0.1307564202964926 1104 70-71 0.1993975660425633 1104 72-73 0.20104795492262184 1104 74-75 0.12166787695236536 1104 76-77 0.12148790438153156 1104 78-79 0.06778716855498601 1104 80-81 0.1806446579697365 1104 82-83 0.16426651313433993 1104 84-85 0.10903781468181961 1104 86-87 0.0012695749828957048 1104 88-89 -0.058936192961585476 1104 90-91 -0.047474236296764616 1104 92-93 0.07585248725177252 1104 94-95 -0.02309080944809594 1104 96-97 -0.03194765976301284 1104 98-99 -0.036687976352421714 1104 100 -0.031113234683335378 1105 1 -0.14930544165902404 1105 2 -0.036770823346419235 1105 3 -0.048604835002972635 1105 4 0.06371367111179893 1105 5 0.003225046827111555 1105 6 0.048439944357539844 1105 7 0.04516949614031063 1105 8 0.08026433856062454 1105 9 0.07158870677342577 1105 10-11 0.08909141568148016 1105 12-13 0.027289963961862895 1105 14-15 0.05852241978514172 1105 16-17 0.025792723933541595 1105 18-19 0.054668156546377134 1105 20-21 0.11122504917597809 1105 22-23 0.0022596042265021765 1105 24-25 0.1824353023069918 1105 26-27 0.015464367467806994 1105 28-29 0.029391897867277805 1105 30-31 0.03187430449624884 1105 32-33 -0.007025415590796058 1105 34-35 -0.06460306582020081 1105 36-37 0.07984554659385878 1105 38-39 0.11641282156733723 1105 40-41 0.1185949918912712 1105 42-43 0.17306002549494792 1105 44-45 0.15931338890415958 1105 46-47 0.09194375312395664 1105 48-49 0.07960119389512954 1105 50-51 0.04139087865605262 1105 52-53 0.07541791827783584 1105 54-55 -0.06393713748229146 1105 56-57 0.031848316816123656 1105 58-59 0.018518679916873282 1105 60-61 0.06749449545696962 1105 62-63 0.04415119033498627 1105 64-65 -0.019520066365402045 1105 66-67 0.06391414426994402 1105 68-69 0.008278922409232337 1105 70-71 0.043631535380640685 1105 72-73 0.03681702954416721 1105 74-75 0.1009408352909773 1105 76-77 0.0353764165073116 1105 78-79 -0.006469421599220482 1105 80-81 0.041580368217168484 1105 82-83 -0.057287983128990305 1105 84-85 0.01412073957735771 1105 86-87 -0.01622387608701814 1105 88-89 0.011714266613747526 1105 90-91 -0.03907978145631574 1105 92-93 -0.01456953456315091 1105 94-95 -0.08190326123059677 1105 96-97 -0.0972988046157397 1105 98-99 -0.1537278194651961 1105 100 -0.2563190792330623 1106 1 -0.1734228147864414 1106 2 -0.09017877058910528 1106 3 -0.09023563163711046 1106 4 0.07368608419825051 1106 5 -0.004738515342644689 1106 6 -0.10247867149960399 1106 7 -0.04842152308057024 1106 8 -0.02564955370195321 1106 9 -0.07018911826549612 1106 10-11 0.04408539191349803 1106 12-13 0.11095854147772855 1106 14-15 0.044055164274546144 1106 16-17 -0.0116338582244353 1106 18-19 0.062157692547870624 1106 20-21 0.01721255937818711 1106 22-23 -0.008986550026513385 1106 24-25 0.22219323140995328 1106 26-27 0.03294084207470149 1106 28-29 0.043346253407648305 1106 30-31 -0.09607534637122939 1106 32-33 -0.04498678858204386 1106 34-35 -0.18055306352968614 1106 36-37 -0.10903507389905087 1106 38-39 -0.053805301057501254 1106 40-41 -0.05553677489353959 1106 42-43 0.05505133227730141 1106 44-45 0.031629983122584804 1106 46-47 -0.06414095195687253 1106 48-49 -0.016064208209968456 1106 50-51 -0.08404775928153896 1106 52-53 -0.0632497582455187 1106 54-55 -0.15836964092507344 1106 56-57 -0.14617429037792107 1106 58-59 -0.057457903388204556 1106 60-61 -0.06704667730977576 1106 62-63 -0.091168090115481 1106 64-65 -0.06195420880126079 1106 66-67 -0.07214076461685437 1106 68-69 -0.018784763551792594 1106 70-71 -0.07035917245515577 1106 72-73 -0.15201839148038943 1106 74-75 -0.08993932281970274 1106 76-77 -0.07502003338485252 1106 78-79 -0.09348710290647233 1106 80-81 -0.06394177073002538 1106 82-83 -0.09377270030051577 1106 84-85 -0.09266878720673333 1106 86-87 -0.1418070015585826 1106 88-89 -0.16705838388252303 1106 90-91 -0.07907900338090457 1106 92-93 -0.06277212457867165 1106 94-95 -0.08949445511732179 1106 96-97 -0.1953047978065925 1106 98-99 -0.17152444563874525 1106 100 -0.1638191268138769 1107 1 -0.17536649342727273 1107 2 -0.15628196738475708 1107 3 -0.12159292341939221 1107 4 -7.77100232618011E-4 1107 5 -0.03523520054175577 1107 6 -0.05414807148824252 1107 7 -0.10329179083883133 1107 8 0.001160503199564289 1107 9 -0.03826617740395477 1107 10-11 -0.07476453288018803 1107 12-13 -0.08202373252552064 1107 14-15 -0.0058402059336089 1107 16-17 -0.10682423166095134 1107 18-19 -0.11217404726723146 1107 20-21 -0.057064085938634435 1107 22-23 -0.05900101229756416 1107 24-25 0.022419839881749226 1107 26-27 -0.061856113393488954 1107 28-29 -0.12283820089345454 1107 30-31 -0.15342269846225776 1107 32-33 -0.12457763055945037 1107 34-35 -0.1118257205896569 1107 36-37 -0.17155434870413444 1107 38-39 -0.1737226279005739 1107 40-41 -0.20130448007802215 1107 42-43 -0.18453536698076078 1107 44-45 -0.21836696626382945 1107 46-47 -0.18777996483224513 1107 48-49 -0.10113602993828152 1107 50-51 -0.1410699117950358 1107 52-53 -0.1827118209678531 1107 54-55 -0.11272879262267566 1107 56-57 -0.33197792164487794 1107 58-59 -0.2875282517882951 1107 60-61 -0.17709834761591026 1107 62-63 -0.26310804464507953 1107 64-65 -0.21047393573645934 1107 66-67 -0.15277397137543858 1107 68-69 -0.13607729799294077 1107 70-71 -0.1454364405363151 1107 72-73 -0.09787996522619835 1107 74-75 -0.09432976033161111 1107 76-77 -0.09754010065456953 1107 78-79 -0.12850533946058107 1107 80-81 -0.11315415094549053 1107 82-83 -0.16287605394912674 1107 84-85 -0.13222350158049068 1107 86-87 -0.21350049291675788 1107 88-89 -0.19142287714197792 1107 90-91 -0.1778598100265114 1107 92-93 -0.21282218938649322 1107 94-95 -0.3872571615205196 1107 96-97 -0.3496881306548829 1107 98-99 -0.26828778214999716 1107 100 -0.26629486581911266 1108 1 -0.1657112142160173 1108 2 -0.11312641003710056 1108 3 -0.10757185666383506 1108 4 0.0017658885469060692 1108 5 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-0.33334947277516846 2215 26-27 -0.6191279467046229 2215 28-29 -0.414229907600685 2215 30-31 -0.3244685297162775 2215 32-33 -0.19180977832535717 2215 34-35 -0.23548412939950225 2215 36-37 -0.3122594939167058 2215 38-39 -0.3193170728010699 2215 40-41 -0.22141071688119496 2215 42-43 -0.13643291384231304 2215 44-45 -0.26725225310229206 2215 46-47 -0.12839825114415504 2215 48-49 -0.009429138779410096 2215 50-51 -0.017053968740889047 2215 52-53 -0.1069430580922699 2215 54-55 -0.16240637433865857 2215 56-57 -0.1679074726337717 2215 58-59 -0.20272708794503558 2215 60-61 -0.21697364551702236 2215 62-63 -0.23582645586468942 2215 64-65 -0.20777566082533383 2215 66-67 -0.07837119592200281 2215 68-69 -0.20706267345656215 2215 70-71 -0.18276459040419013 2215 72-73 -0.13268218227110395 2215 74-75 -0.11263852458693435 2215 76-77 -0.022009756932376945 2215 78-79 0.013329452708212841 2215 80-81 0.12777885368814168 2215 82-83 0.07243908993661208 2215 84-85 0.10138186517229286 2215 86-87 0.08228782675443114 2215 88-89 0.08583398155661115 2215 90-91 0.0025934300123040543 2215 92-93 0.03126977769334616 2215 94-95 -0.017956513116168082 2215 96-97 0.06573709346415768 2215 98-99 0.1980140106158963 2215 100 0.25094050202265095 2216 1 0.019909934128502726 2216 2 0.005232166544484329 2216 3 -0.05992716474935733 2216 4 -0.03660113754932581 2216 5 -0.05298830061662585 2216 6 -0.119397766928941 2216 7 -0.06993559915954961 2216 8 -0.02346744523138966 2216 9 -2.1229807534361314 2216 10-11 -0.6142382665901849 2216 12-13 -0.39437741923201486 2216 14-15 -0.345501298966191 2216 16-17 -0.5168770440064421 2216 18-19 -0.524232860100085 2216 20-21 -0.6761057541240092 2216 22-23 -0.16479596223786785 2216 24-25 0.05453405403115141 2216 26-27 -0.07609404346631976 2216 28-29 -0.11556304085980429 2216 30-31 -0.10104186832770523 2216 32-33 -0.09413960967484769 2216 34-35 -0.058701154632828434 2216 36-37 -0.28517438293231123 2216 38-39 -0.22287176906169748 2216 40-41 -0.1527477365854395 2216 42-43 -0.14196759287304417 2216 44-45 -0.18172404474844228 2216 46-47 -0.18596801809917451 2216 48-49 -0.14093793813185584 2216 50-51 -0.27915083841255495 2216 52-53 -0.283257638146857 2216 54-55 -0.26216980858433203 2216 56-57 -0.16516148296226163 2216 58-59 -0.20797800094589292 2216 60-61 -0.1457410603667455 2216 62-63 -0.11413622620452912 2216 64-65 -0.193060114384906 2216 66-67 -0.14522472713983348 2216 68-69 -0.21016724782445806 2216 70-71 -0.18864294052475827 2216 72-73 -0.1475381899662409 2216 74-75 -0.19385147880777254 2216 76-77 -0.22907152183861967 2216 78-79 -0.19117649399998982 2216 80-81 -0.14598515164173165 2216 82-83 -0.20981954232969713 2216 84-85 -0.0954363742965576 2216 86-87 -0.07730734356667313 2216 88-89 -0.0452872353215632 2216 90-91 -0.047862941494777544 2216 92-93 -0.08160212890531682 2216 94-95 -0.07557587669338162 2216 96-97 -0.06503562897784576 2216 98-99 -0.11313915466592306 2216 100 -0.13809426649693535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 241.0 3 46.0 4 113.0 5 333.0 6 1001.0 7 2504.0 8 4971.0 9 7340.0 10 10533.0 11 14731.0 12 19423.0 13 24062.0 14 26068.0 15 31432.0 16 39378.0 17 49340.0 18 46138.0 19 32145.0 20 29860.0 21 31790.0 22 35416.0 23 41673.0 24 51758.0 25 66483.0 26 87221.0 27 113911.0 28 151088.0 29 197454.0 30 253435.0 31 323242.0 32 408104.0 33 521734.0 34 688049.0 35 949881.0 36 1406401.0 37 2045756.0 38 1770105.0 39 388677.0 40 562.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.646498409390375 18.756375914818385 13.351120033169956 24.246005642621284 2 17.52603394575118 19.60661233404363 36.98910467455783 25.878249045647365 3 18.72279196925949 24.46032161656106 29.72818432150919 27.088702092670257 4 13.724536457653302 17.071402806957053 35.82195168570476 33.382109049684885 5 14.206273700707897 34.92885178667732 33.02644390212871 17.83843061048607 6 31.419797083279814 35.55012563267122 17.84513732070348 15.184939963345487 7 27.77621731050376 30.75134017577693 21.90196121530339 19.570481298415917 8 26.483350196846782 33.15988342853647 20.349410513088053 20.00735586152869 9 25.39164999307666 16.44121150289813 21.144870664161772 37.022267839863446 10-11 24.97715094375744 25.194210647280364 27.64681613861028 22.181822270351915 12-13 25.85982428782479 22.967365061872016 27.78190094703464 23.39090970326856 14-15 23.353749350904753 24.61192929314876 25.56319790875477 26.47112344719172 16-17 22.82365626577948 27.28598784793356 25.87532711160767 24.015028774679294 18-19 22.72352950888634 26.099770683903678 28.32181418113267 22.85488562607731 20-21 23.789015213019653 25.56914990976357 27.911777066546843 22.73005781066993 22-23 23.377509357148146 25.624825333741068 27.85722092472154 23.140444384389244 24-25 23.14970251911415 25.55538425867917 28.20110897057544 23.09380425163124 26-27 23.271785377269623 25.31635305696026 28.145409261983662 23.26645230378645 28-29 23.270565816248933 25.335371139097262 28.253501294798767 23.14056174985504 30-31 23.417590607818862 25.895979308742305 27.763132544911656 22.923297538527176 32-33 23.247120583355677 25.57655945631857 28.06099611654675 23.115323843779002 34-35 23.152072763671725 25.06282414233866 28.13800880616758 23.64709428782204 36-37 23.616377336450846 25.49711574663868 27.94456038496823 22.941946531942236 38-39 23.48725978356426 25.55220873872703 27.797833130528858 23.162698347179848 40-41 23.356075863627474 25.238622344984236 27.971397833495182 23.43390395789311 42-43 23.03400738938972 25.569642473407043 27.979416552117502 23.41693358508573 44-45 23.254266769404275 25.6526048025409 27.746229665150285 23.346898762904537 46-47 23.338618100828377 25.73590775656454 27.356380146304865 23.569093996302215 48-49 23.271357171783393 25.58545552964943 27.5452681303975 23.59791916816968 50-51 23.25789539684279 25.505130567061823 27.385823586123653 23.85115044997173 52-53 23.200819780480913 25.62539257175485 27.358314833101865 23.81547281466237 54-55 23.217441880134707 26.063340835393706 27.218384305577604 23.500832978893985 56-57 22.772423399824095 25.82931970233375 27.260643537604185 24.13761336023797 58-59 22.820476562991427 25.39530665241549 27.595111380729243 24.18910540386384 60-61 23.189642152834384 25.83202927677457 27.24192974777458 23.73639882261647 62-63 22.923450520992922 25.95178233780867 27.222729753933162 23.902037387265242 64-65 22.905587588183987 25.49151427125261 27.3984317287014 24.204466411862 66-67 22.60252769038541 25.716905465028013 27.32888368910984 24.351683155476742 68-69 23.118387941978437 25.728098104624824 27.05398657408397 24.099527379312768 70-71 23.011392671629256 25.423678682354716 27.21943774760319 24.345490898412837 72-73 22.721422624835157 25.785971575905712 27.271299508863045 24.221306290396083 74-75 22.778814956729363 25.585230094529205 27.12038887407205 24.51556607466939 76-77 22.906889196840606 25.70201528524121 26.763064377766742 24.628031140151446 78-79 22.84587753570663 26.01485495516995 26.966619856010638 24.17264765311278 80-81 22.72639608670598 25.759726688518157 26.97343371150214 24.540443513273726 82-83 22.72598654237601 25.727557640133274 26.988619227357812 24.557836590132904 84-85 22.78747226103977 25.87314670116555 26.674076890756815 24.665304147037862 86-87 22.980505032517947 25.874063575663612 26.655054488303442 24.490376903514992 88-89 22.721459455528215 25.699541570160182 26.80014087768377 24.77885809662783 90-91 22.405080632683514 25.71497739782935 26.8106489890875 25.06929298039963 92-93 22.719213062121817 25.834156926289296 26.58272416829435 24.86390584329453 94-95 22.697322123013457 25.540108493468882 26.74345927225435 25.019110111263316 96-97 22.387695426256286 25.628743139708565 26.835405880275847 25.1481555537593 98-99 22.446180609982715 25.68122657578159 26.86115308702463 25.011439727211066 100 22.518509694811613 25.489886242384575 26.79707677793248 25.194527284871327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1964.0 1 1912.5 2 9290.5 3 13758.5 4 7102.0 5 3013.0 6 3465.0 7 7516.0 8 12141.5 9 13361.5 10 10174.5 11 6238.0 12 5302.5 13 5580.5 14 6255.0 15 6953.5 16 7538.5 17 8414.0 18 9413.5 19 10418.0 20 11723.0 21 13381.0 22 15660.5 23 19048.5 24 23924.5 25 30222.5 26 38495.0 27 49158.5 28 62635.0 29 78744.5 30 94028.0 31 108675.0 32 122526.5 33 135319.5 34 149723.0 35 165903.5 36 184143.0 37 204393.5 38 223101.5 39 238210.0 40 254158.5 41 270557.0 42 282029.0 43 291953.0 44 302128.0 45 312480.5 46 324178.0 47 330511.5 48 333920.0 49 344997.0 50 358595.0 51 366969.0 52 370128.0 53 376827.0 54 382253.5 55 371754.5 56 350571.5 57 321640.5 58 291863.0 59 268756.0 60 241385.5 61 208179.0 62 177526.5 63 146853.0 64 115959.0 65 90801.0 66 69648.5 67 51801.0 68 39033.5 69 29483.0 70 21943.5 71 16124.5 72 12176.5 73 9110.0 74 6974.0 75 4754.5 76 2590.5 77 1564.5 78 1070.0 79 638.0 80 315.0 81 149.0 82 79.5 83 36.0 84 18.0 85 15.5 86 14.0 87 12.0 88 9.0 89 6.5 90 4.5 91 2.5 92 2.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032687090544051146 2 0.0 3 8.508570206694441E-4 4 0.0 5 0.0033527818314474525 6 0.007728617937747451 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.051700098425925E-5 14-15 3.545237586122684E-5 16-17 4.051700098425925E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.355577605807869E-4 28-29 1.570033788140046E-4 30-31 1.7219725418310178E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 5.064625123032406E-6 44-45 0.0 46-47 0.0 48-49 1.0129250246064812E-5 50-51 1.0129250246064812E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.02351505444623946 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.0387750738194435E-5 80-81 0.0 82-83 2.5323125615162028E-5 84-85 3.0387750738194435E-5 86-87 5.0646251230324056E-5 88-89 5.0646251230324056E-5 90-91 6.584012659942127E-5 92-93 6.077550147638887E-5 94-95 7.596937684548608E-5 96-97 5.0646251230324056E-5 98-99 4.558162610729165E-5 100 5.0646251230324056E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9872399.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.0226881765042 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59123814905017 34.134351558291414 2 14.869417712673801 13.983999849384968 3 4.847918278227879 6.838864485068538 4 2.2422638678057756 4.217490986610929 5 1.257442455940059 2.9564162252783506 6 0.8020962332570359 2.2630032638396504 7 0.5712337327297309 1.8802661983035471 8 0.4144955726202099 1.5592556849489343 9 0.30543928402984266 1.292631858881099 >10 1.856846555948755 16.365028763714545 >50 0.1535633632054182 4.968011573428243 >100 0.08409417329873994 7.01657511537502 >500 0.003087861421062501 0.9721284424649778 >1k 7.117770957147065E-4 0.5139518328682773 >5k 6.470686965838702E-5 0.22797869094178208 >10k+ 8.62758262111827E-5 0.8100454705996197 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23120 0.23418826568901846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21837 0.22119243762331728 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20682 0.20949315358911244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14229 0.1441291017512562 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9980 0.10108991745572682 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0129250246064812E-5 0.0 2 0.0 0.0 0.0 1.0129250246064812E-5 0.0 3 0.0 0.0 0.0 1.0129250246064812E-5 0.0 4 0.0 0.0 0.0 2.0258500492129625E-5 0.0 5 0.0 0.0 0.0 3.0387750738194435E-5 0.0 6 0.0 0.0 0.0 4.051700098425925E-5 0.0 7 0.0 0.0 0.0 4.051700098425925E-5 0.0 8 0.0 0.0 0.0 5.0646251230324056E-5 0.0 9 0.0 0.0 0.0 9.11632522145833E-5 0.0 10-11 0.0 0.0 0.0 9.11632522145833E-5 0.0 12-13 0.0 1.0129250246064812E-5 0.0 1.2155100295277774E-4 0.0 14-15 0.0 1.0129250246064812E-5 1.0129250246064812E-5 1.671326290600694E-4 0.0 16-17 0.0 1.0129250246064812E-5 1.0129250246064812E-5 1.9245575467523142E-4 0.0 18-19 5.064625123032406E-6 1.0129250246064812E-5 1.0129250246064812E-5 2.4310200590555548E-4 0.0 20-21 1.0129250246064812E-5 1.5193875369097218E-5 1.0129250246064812E-5 3.646530088583332E-4 0.0 22-23 1.0129250246064812E-5 2.0258500492129625E-5 1.0129250246064812E-5 0.0010179896497295136 0.0 24-25 1.0129250246064812E-5 2.532312561516203E-5 1.0129250246064812E-5 0.00242089080880949 0.0 26-27 1.0129250246064812E-5 3.0387750738194435E-5 1.0129250246064812E-5 0.004907621744218401 0.0 28-29 3.0387750738194435E-5 3.545237586122684E-5 1.0129250246064812E-5 0.011486569779037496 0.0 30-31 3.0387750738194435E-5 4.051700098425925E-5 1.0129250246064812E-5 0.030681498995330313 0.0 32-33 3.0387750738194435E-5 4.051700098425925E-5 1.0129250246064812E-5 0.0742068873026708 0.0 34-35 3.0387750738194435E-5 4.051700098425925E-5 1.0129250246064812E-5 0.13990520439864718 0.0 36-37 3.0387750738194435E-5 5.571087635335647E-5 1.0129250246064812E-5 0.23901485343126833 0.0 38-39 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 0.37953287746980247 0.0 40-41 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 0.5495726013504925 0.0 42-43 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 0.7277157254280342 0.0 44-45 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 0.9361149199905716 0.0 46-47 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 1.1812478405704632 0.0 48-49 3.0387750738194435E-5 7.090475172245368E-5 1.0129250246064812E-5 1.4476420574168447 0.0 50-51 3.0387750738194435E-5 8.10340019685185E-5 1.0129250246064812E-5 1.7254519392905412 1.0129250246064812E-5 52-53 3.0387750738194435E-5 8.10340019685185E-5 1.0129250246064812E-5 2.0066601846217926 1.0129250246064812E-5 54-55 3.0387750738194435E-5 9.622787733761571E-5 1.0129250246064812E-5 2.3079901855668514 1.0129250246064812E-5 56-57 3.0387750738194435E-5 1.0129250246064811E-4 1.0129250246064812E-5 2.6478164020720802 1.0129250246064812E-5 58-59 3.0387750738194435E-5 1.0129250246064811E-4 1.0129250246064812E-5 3.01263147893435 1.0129250246064812E-5 60-61 3.0387750738194435E-5 1.0129250246064811E-4 1.0129250246064812E-5 3.388153173306711 1.0129250246064812E-5 62-63 3.0387750738194435E-5 1.0129250246064811E-4 1.0129250246064812E-5 3.7677822786538506 1.0129250246064812E-5 64-65 3.0387750738194435E-5 1.0129250246064811E-4 1.0129250246064812E-5 4.178219498624397 1.0129250246064812E-5 66-67 3.0387750738194435E-5 1.1142175270671293E-4 1.0129250246064812E-5 4.632060555899331 1.0129250246064812E-5 68-69 3.0387750738194435E-5 1.1142175270671293E-4 1.0129250246064812E-5 5.103485991601433 1.0129250246064812E-5 70-71 3.0387750738194435E-5 1.2155100295277774E-4 1.0129250246064812E-5 5.591462622205606 1.5193875369097218E-5 72-73 3.0387750738194435E-5 1.4180950344490736E-4 1.0129250246064812E-5 6.112501125612933 2.0258500492129625E-5 74-75 3.0387750738194435E-5 1.4180950344490736E-4 1.0129250246064812E-5 6.616081866221169 2.0258500492129625E-5 76-77 3.0387750738194435E-5 1.4180950344490736E-4 1.0129250246064812E-5 7.142691457263832 2.0258500492129625E-5 78-79 3.0387750738194435E-5 1.5193875369097216E-4 1.0129250246064812E-5 7.705188981928304 2.0258500492129625E-5 80-81 3.0387750738194435E-5 1.5193875369097216E-4 1.0129250246064812E-5 8.291966319432593 2.0258500492129625E-5 82-83 3.0387750738194435E-5 1.570033788140046E-4 2.0258500492129625E-5 8.907080234500246 2.0258500492129625E-5 84-85 3.0387750738194435E-5 1.671326290600694E-4 2.0258500492129625E-5 9.504331216758967 2.0258500492129625E-5 86-87 3.0387750738194435E-5 1.823265044291666E-4 2.0258500492129625E-5 10.116745686636044 2.0258500492129625E-5 88 3.0387750738194435E-5 1.9245575467523142E-4 2.0258500492129625E-5 10.60259011006342 2.0258500492129625E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 1545 0.0 51.717983 2 GGTATCA 14915 0.0 49.02275 1 ATAACGC 1770 0.0 47.002514 3 TAACGCA 2020 0.0 42.116116 4 GTATCAA 24690 0.0 38.50797 1 ATCAACG 30210 0.0 30.89937 3 TCAACGC 30540 0.0 30.61166 4 TACCTGG 8300 0.0 29.730509 2 GTATAAC 3465 0.0 29.71881 1 CAACGCA 31935 0.0 29.200876 5 TATCAAC 32195 0.0 29.08185 2 AACGCAG 34100 0.0 27.773373 6 ACGCAGA 38665 0.0 24.480404 7 CGCAGAG 39905 0.0 23.684372 8 TATCACG 2030 0.0 23.385489 2 GTACCTG 10885 0.0 23.326836 1 GCAGAGT 43835 0.0 21.432306 9 GTGGTAT 7760 0.0 21.389643 1 AGAGTAC 40010 0.0 21.161325 10-11 ACCTGGG 11570 0.0 20.637232 3 >>END_MODULE