FastQCFastQC Report
Wed 25 May 2016
SRR2049485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10021620
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA201410.20097549098848289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14700.055.022642
ATAACGC18900.043.541823
GTATAAC25750.037.072261
TAACGCA26300.034.3299874
GGTATCA95750.034.0840341
TGTCACG30500.030.0461987
GTCACGC30050.029.2447268
GTATCAA141300.029.2201651
GCTACGC29100.028.7645342
TCTGTCG46100.028.6454628
CGACTCG11300.026.2174385
TACCTGG67350.025.8341662
GTCTCGC47700.025.730751
GGCTACG39950.025.543081
TCAACGC153650.025.310524
ATCAACG154700.025.1084583
TCGCTCT48500.025.0153224
GTACATG344800.024.19451
CAACGCA165500.023.6119125
TCACGCA37500.023.4338389
Produced by FastQC (version 0.11.5)