FastQCFastQC Report
Wed 25 May 2016
SRR2049485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10021620
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492700.49163708063167433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350450.34969396165490213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336080.3353549625709217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231590.23109038259283432No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217800.21733013225406672No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT115340.1150911728842243No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102260.10203939083701039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG20450.067.579442
ATAACGC26200.054.5422253
GGTATCA207000.052.0216261
GTATAAC29850.051.5136381
TAACGCA30000.048.8863224
GTATCAA344700.037.43361
TATCACG18600.033.3596532
GTCACGC29200.032.9950838
GCTACGC28400.031.1171682
TACCTGG71000.030.057862
TGTCACG33050.029.4358847
ATCAACG438900.029.067313
TCAACGC442550.028.8590054
TCTGTCG44100.028.5610688
TATCAAC450800.028.3417132
CAACGCA457550.027.789635
GTATACG4100.027.5261961
GTGGTAT96050.027.5142561
CGGATAC22050.026.8606194
AACGCAG486950.026.447566
Produced by FastQC (version 0.11.5)